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Citations for Agilent :
1051 - 1100 of 1403 citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... The assembly products were then re-purified using the Monarch PCR & DNA Cleanup Kit and quantified by Agilent Bioanalyzer (DNA 1000).
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bioRxiv - Cell Biology 2020Quote: ... All cell lines were tested regularly for mycoplasma using the PCR-based assay (Stratagene, San Diego, CA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... The qPCR reactions were performed using the Agilent MXPro Real-Time PCR detection system (Agilent Technologies, Stratagene, USA). A total volume of 20µl contained10μl Brilliant III SYBR Green ...
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bioRxiv - Molecular Biology 2021Quote: ... The qPCR reactions were performed using the Agilent MXPro Real-Time PCR detection system (Agilent Technologies, Stratagene, USA). A total volume of 20µl contained10μl Brilliant III SYBR Green ...
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bioRxiv - Immunology 2021Quote: ... The gRNAs were amplified by a two-step PCR method using the Herculase II Fusion DNA Polymerase (Agilent). For the first step PCR ...
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bioRxiv - Plant Biology 2019Quote: ... Gene transcript levels were measured by qRT-PCR using a Mx3000P QPCR System (Agilent Technologies, Santa Clara, CA). All reactions were made with the Brilliant SYBR Green Master Mix (Stratagene Inc. ...
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bioRxiv - Genomics 2021Quote: ... PCR products were analysed by capillary gel electrophoresis on the TapeStation 2200 system using D1000 Screen Tapes (Agilent) according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2020Quote: ... CiF1 was amplified from BSK-CiF1 by long-range PCR using PfuUltraII Fusion HS DNA polymerase (Agilent Technologies), adding RsrII and PmeI at either end and cloning the product into a Zero Blunt Topo cloning vector (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... Real-time PCR reactions were performed with the Brilliant II SYBR® Green QPCR Master Mix (Agilent, 600828). The relative quantification in gene expression was carried out using the 2- ΔΔCt method (51) ...
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bioRxiv - Immunology 2020Quote: ... The PCR product was digested in a 50ul reaction as previously described and full digestion confirmed by Agilent Bioanalyzer ...
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bioRxiv - Immunology 2021Quote: ... RACE products were subjected to gel-purification and sub-cloned using the Strataclone UA PCR cloning kit (Agilent). Insert sequences were determined by Sanger sequencing (Seqlab GmbH).
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bioRxiv - Genetics 2020Quote: ... The DNA samples were then enriched by PCR with Herculase II polymerase (Agilent Technologies, Santa Clara, CA, USA). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... While the first polymerase chain reaction (PCR) was performed on a SureCycler 8800 (Agilent Technologies, Santa Clara, USA) using the following temperature profile ...
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bioRxiv - Plant Biology 2022Quote: Quantitative real-time PCR (qPCR) was performed using an Mx3000P Multiplex qPCR system (Stratagene, La Jolla, CA, USA) as described before (Cabello et al. ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... The sgRNA sequences found in the genomic DNA and viral RNA samples were amplified by PCR (Agilent; 600677) and RT-PCR (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... a full-length cDNA for SBT3.3 was amplified by PCR using Pfu DNA polymerase (Stratagene, San Diego, CA) and specific primers ...
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bioRxiv - Plant Biology 2022Quote: ... was used for a qRT-PCR reaction carried out in a Stratagene Mx3005P qPCR machine (Agilent Technologies, USA). Five independent RNA isolations and three technical replicates were used for mRNA quantification ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative-PCR assays were performed on cDNAs samples (diluted 1/50e) in an Mx30005P thermal cycler (Stratagene, USA) using the Brilliant III Ultra-fast SYBR® Green QPCR Master mix with low ROX (Agilent ...
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bioRxiv - Plant Biology 2022Quote: ... was used for a qRT-PCR reaction carried out in a Stratagene Mx3005P qPCR machine (Agilent Technologies, USA). Three independent RNA isolations along with three technical replicates were used for mRNA quantification ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were then purified and checked for fragment size distribution using D1000 DNA ScreenTapes (Agilent Technologies, US). Sample libraries were multiplexed and sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... 10-µl reactions were prepared with the Brilliant III Ultra-Fast SYBR Green qRT-PCR Master Mix (Agilent) in technical duplicates for each sample ...
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bioRxiv - Molecular Biology 2024Quote: ... Expression plasmids were constructed with the obtained PCR product using the SureVector system (Agilent, Santa Clara, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... cDNA samples were then subjected to two rounds of PCR using Herculase II fusion DNA polymerase (Agilent Technologies).
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bioRxiv - Plant Biology 2022Quote: Quantitative real-time PCR (qPCR) was performed using an Mx3000P Multiplex qPCR system (Stratagene, La Jolla, CA, USA) as described before (Mora et al. ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR products were purified (AMPure XP system) and resulting libraries were analyzed for size distribution by Agilent 2100 Bioanalyzer and quantified using real-time PCR.
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bioRxiv - Cell Biology 2023Quote: ... Von Zastrow lab).The DNA fragments were amplified by Pfu Ultra II Hotstart PCR master mix (Agilent Technologies) and ligated with each respective vector by NEBbuilder HiFi DNA assembly master mix (New England BioLabs).
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bioRxiv - Systems Biology 2023Quote: ... The wPCR reaction was carried out on a AriaMx Real-Time PCR System (Agilent, Santa Clara, CA, USA) with 40 cycles of 15 s at 95 °C and 30 s at 60 °C ...
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bioRxiv - Biophysics 2023Quote: ... Site-directed mutagenesis was performed using standard two-step PCR mutagenesis using the Herculase II DNA Polymerase (Agilent), with sequences verified by Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: ... Gene expression was quantified by performing quantitative real-time PCR (qPCR) in a Mx3005P qPCR system (Agilent Technologies) using GoTaq® qPCR Master Mix (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... The qPCR was performed in 20 μl SYBR Green reaction mixture using a Real-time PCR system (Agilent AriaMx Real-time PCR System ...
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bioRxiv - Microbiology 2023Quote: ... The amplification of the fragments was made by PCR and the template size distribution was checked by Agilent Technologies 2100 Bioanalyzer using a DNA 1000 chip ...
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bioRxiv - Microbiology 2023Quote: ... DNA molarity and fragment sizes of the resulted PCR products were measured on a fragment analyzer 5300 (Agilent) and subsequently ...
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bioRxiv - Genetics 2022Quote: ... The PCR products were purified (AMPure XP system) and resulting libraries were analyzed for size distribution by Agilent 2100 Bioanalyzer (Panaro et al ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA levels were measured by quantitative realtime PCR using Brilliant III Ultra-Fast SYBR Green qPCR mix (Agilent) and 24 ng of cDNA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PCR products were purified (AMPure XP system) and resulting libraries were analyzed for size distribution by Agilent 2100 Bioanalyzer and quantified using real-time PCR.
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bioRxiv - Molecular Biology 2023Quote: ... The 2nd PCRs were quantified with Qubit and their quality and size were checked with a Bioanalyzer (Agilent). The 20 PCRs corresponding to the 20 different samples were pooled in an equimolar manner and purified twice with the SPRI based size selection following the kit instructions for selection of the fragment between 400 to 1000bp ...
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bioRxiv - Cell Biology 2024Quote: ... library preparation and post-capture PCR were performed according to manufacturer’s recommendations using the Agilent capture kit (Agilent #5190-4816 with the SureSelect design #3132861) ...
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bioRxiv - Molecular Biology 2024Quote: ... The cDNA content and size of post-sample index PCR samples was analyzed using a 2100 BioAnalyzer (Agilent). Library quantification was done using NEB Next® Library Quant Kit for Illumina® (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... A total of 10 ng of the reconstituted oligos was utilized for PCR amplification (Agilent Cat No. 600679). Two PCR reactions were carried out ...
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bioRxiv - Genetics 2024Quote: ... PCR-2 reactions amplified vector fragments from MSp509_PKR-WT plasmid using Herculase II polymerase (Agilent Technologies Cat#600677) using cycling conditions for vector targets >10 kb ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were purified via AMPure XP bead cleanup and quantified using a Bioanalyzer (Agilent Technologies, Santa Clara, CA). A pool consisting of an equimolar quantity of each library was sequenced as 100 bp SE reads on the Illumina HiSeq2500 platform at the University of California ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We produced the promoter inserts by performing error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies). We used the plasmid constructs with MG1655 promoter variants cloned into them as template DNA for the error-prone PCR aiming to achieve approximately 1.5 SNPs per promoter sequence ...
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bioRxiv - Genomics 2020Quote: ... and quantitative PCR was performed in triplicate using the PowerUp SYBR Green Master Mix on either an AriaMx (Agilent) or a StepOnePlus (Applied Biosystems ...
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bioRxiv - Physiology 2019Quote: ... Final libraries were amplified for 14 cycles by PCR and the final size assessed using a 2100 bioanalyzer (Agilent) with a HS DNA chip (Agilent) ...
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bioRxiv - Cancer Biology 2019Quote: ... The concentration and size distribution of Hi-C library DNA after PCR amplification was determined by tapestation (Agilent Technologies), and the Hi-C libraries were sequenced on Illumina Hi-Seq 2500 or Illumina Hi-seq 4000 at 50 cycles.
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bioRxiv - Genetics 2020Quote: ... Sequencing libraries were prepared by PCR amplification (30 cycles, annealing temperature 55C) using PfuUltra II Fusion DNA polymerase (Agilent) and custom primers that were designed to anneal to a 3’ site within the EGFP gene (F ...
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bioRxiv - Cell Biology 2019Quote: ... The relative gene abundance in the cDNA sample was examined by the quantitative PCR (qPCR) experiment on MX3000 (Agilent) using PerfeCTa SYBR green PCR mix (Quanta Bioscience) ...
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bioRxiv - Molecular Biology 2019Quote: ... Each PCR fragment was subcloned into the EcoRV recognition site of pBluescript KS (+) vector (Stratagene, La Jolla, CA, USA) using DNA Ligation kit Ver ...
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bioRxiv - Developmental Biology 2019Quote: ... Arg-177-Ala mutants were generated in pCR II-TOPO TA vector using QuikChange site directed mutagenesis kit (Agilent). The wild type and the respective modifications were confirmed by DNA sequencing ...
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bioRxiv - Biochemistry 2021Quote: ... Selected residues were mutated to alanine or oppositely-charged amino acids by PfuUltra II Hotstart PCR Master Mix (Agilent). All mutated sequences were confirmed by Sanger sequencing ...