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Citations for Agilent :
1301 - 1341 of 1341 citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... All UTX isoforms and mutants were generated by PCR cloning into the pDONR221 vector or by site-directed mutagenesis using the QuikChange strategy (Agilent, CA, USA). Inserts were transferred to the pCDNA5_FRT_TO_N-GFP destination vector using GATEWAY cloning according to instructions of the manufacturer (Life technologies ...
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bioRxiv - Systems Biology 2023Quote: ... 7 cycles were used for the Sample Index PCR reaction and final libraries were evaluated using D5000 ScreenTape (Agilent 4200 TapeStation System) and DNA 7500 (Agilent 2100 Bioanalyzer) ...
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bioRxiv - Systems Biology 2023Quote: The genomic integrated sgRNA libraries were amplified and indexed in two rounds of PCR using the Herculase II Fusion DNA Polymerase kit (Agilent Technologies 600679). The libraries were sequenced on an Illumina NextSeq with a custom primer oMCB1672 for read 1 and ≥21 cycles in read 1 ...
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bioRxiv - Physiology 2023Quote: ... Mutations were introduced by PCR-based site-directed mutagenesis method using the Quick Change II XL Site-Directed Mutagenesis Kit (Agilent Technologies, USA) and verified by sequencing (Supplementary Table 2).
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bioRxiv - Cancer Biology 2023Quote: Mitochondrial DNA copy number was quantified by qPCR on total DNA from gastrocnemius muscle using MxPro Mx3000P real-time PCR Analyzer (Agilent technologies / Stratagene) and determining the mtDNA/nDNA ratio ...
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bioRxiv - Plant Biology 2024Quote: Quantitative PCR (qPCR) was carried out in a 25 μL reaction volume using the Brilliant II SYBR Green PCR Master Mix (Stratagene, Agilent technologies). Primer concentrations were set at 0.2 μM ...
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bioRxiv - Molecular Biology 2024Quote: ... Amplification and melting curve analysis (see supplementary Figure S1) and monitoring were performed using Agilent AreaMx Real-Time PCR System (Agilent Technologies, USA).
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bioRxiv - Molecular Biology 2024Quote: ... Purified mRNA was transferred to a clean nuclease-free PCR tube before the yield and size distribution was assessed using an RNA Pico Chip (Agilent Technologies, Inc.).
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bioRxiv - Microbiology 2019Quote: ... Complementation of ΔfimB mutants were performed by cloning the promoter and encoding regions of the parental fimB genes into pSC-A-amp/kan (Table S4) by using the StrataClone PCR Cloning Kit (Agilent Technologies, Massy, France). The recombinant plasmid was then electroporated into competent strains S250ΔfimB: ...
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bioRxiv - Molecular Biology 2021Quote: ... IMAGE:6292760)) cloned in the pOTB7 vector (IRAUp969D10104D, RZPD Imagenes, Germany) served as template for site-directed PCR-mutagenesis (QuickChange, Stratagene, La Jolla, CA) according to the manufacturer’s instructions and as described in [52] and using the primer pairs (10 nmol ...
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bioRxiv - Physiology 2019Quote: ... amplified products were concentrated using a solid-phase reversible immobilization method for the purification of PCR products and quantified by electrophoresis using an (Agilent, Santa Clara, CA) 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2019Quote: ... Site-directed mutagenesis of the spaO gene was performed according to the QuikChange PCR site-directed mutagenesis protocol (Agilent, Santa Clara, CA, USA). All primers used in this study can be found in Table S5.
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bioRxiv - Cell Biology 2020Quote: pSecTagNC + ΔEGF_mN1 W1758A and pSecTagNC + ΔEGF_mN1 R1994A: these plasmids were generated by PCR-based mutagenesis using the QuikChange Lightning Multi Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pSecTagNC + ΔEGF_mN1 as a template and primers ...
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bioRxiv - Cell Biology 2020Quote: pcDNA3 + mNICD R1994A: this plasmid was generated by PCR-based mutagenesis using the QuikChange Lightning Multi Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3 + mNICD as a template and the primer A2026VmutF.
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bioRxiv - Plant Biology 2019Quote: ... DNA fragments carrying both adapters after sodium bisulfite conversion were enriched by 8 cycles of PCR using the Pfu Turbo Cx Hotstart DNA Polymerase (Agilent, Santa Clara, CA). Primer dimers were subsequently removed using two rounds of purification using AMPure beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2019Quote: ... The anc-1 3’UTR RNAi construct was generated by PCR amplifying part of the 3’UTR-region of the anc-1 transcript using Paq5000™ DNA Polymerase (Agilent Technologies, 600682). The primer sequences are detailed in Table S2 ...
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bioRxiv - Neuroscience 2020Quote: ... Fragment size was determined by loading 1 ng/μl cleaned up PCR product on the Fragment Analyzer System (Agilent Technologies, Santa Clara, USA) using the qualitative DNA Kit dsDNA Reagent 35-5000bp (Cat No ...
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bioRxiv - Microbiology 2020Quote: ... Full-length Vero-PVR1 and Vero-PVR2 cDNA was amplified with the Easy-A High Fidelity PCR Cloning Enzyme (Agilent Technologies, CA, USA), and cloned into T-vector pMD20 (Takara Bio Inc.) ...
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bioRxiv - Cell Biology 2021Quote: Both PCRs were performed in 100 µl with the 2 µl of the Herculase II Fusion DNA Polymerase from Agilent (ref. no. 600675) according the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... from the pIdsBB expression system containing a C-terminal GFPmut2 fusion (Gibbs et al., 2008) using error-prone PCR with the GeneMorph II Random Mutagenesis Kit (Agilent, Santa Clara, CA) and ligated back into the same pIdsBB expression vector using the restriction enzymes SacI and BamHI ...
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bioRxiv - Plant Biology 2021Quote: ... The genomic SPL7 (AT5G18830.1) coding region (translational start to stop codon) was PCR-amplified and cloned into the pBluescript SK+ vector (Stratagene/Agilent Technologies, Waldbronn, Germany) which was used a PCR template for site-directed mutagenesis (A279T ...
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bioRxiv - Cancer Biology 2022Quote: ... The locus-specific HDR donors were generated by PCR amplification of the MACHETE bicistronic cassette using a high-fidelity DNA polymerase (Herculase II, Agilent or Q5, NEB). PCR fragments were column purified (Qiagen ...
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bioRxiv - Systems Biology 2023Quote: ... A total of 13 cycles was then used for the Sample Index PCR reaction and final libraries were evaluated using D5000 ScreenTape (Agilent 4200 TapeStation System). Each type of library was pooled and sequenced using Illumina NovaSeq6000 System on SP flowcells [61] ...
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bioRxiv - Biochemistry 2023Quote: ... was performed in duplicate using TaqManTM Fast Advanced Master Mix (Cat # 44-445-57) for mRNA in a Mx3000p Real-Time PCR System (Stratagene, Santa Clara, CA). The primers for the qPCR were purchased from Thermo Fisher Scientific/TaqMan ...
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bioRxiv - Genomics 2023Quote: ... The PCR products obtained were purified (AMPure XP system) and library quality was assessed on an Agilent Bioanalyzer 2100 system (Agilent Technologies, United States). Library preparations of RNA were sequenced on the Illumina NovaSeq 6000 platform (Illumina Inc ...
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bioRxiv - Microbiology 2023Quote: ... Sequence encoding the 96-120 NS2B residues was removed from recombinant pTrcHisA plasmid by PCR based site directed mutagenesis [40] using Pfu ultra II fusion HS DNA polymerase (Agilent, Santa Clara, USA) to generate recombinant plasmids expressing N-terminal hexahistidine tag fused active NS2B(H)-NS3(pro ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... and retrotranscribed as previously described.16 cDNAs were quantified by quantitative real-time PCR on a MX3000 apparatus (Stratagene, La Jolla, CA, USA) by using specific primers ...
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bioRxiv - Microbiology 2024Quote: ... Quality assessment and quantification of the PCR products were respectively performed using the Agilent 2100 Bioanalyzer system (Agilent Technologies, Palo Alto, CA, USA) and a Qubit 2.0 Fluorometer (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... analysis was performed using TransStart Tip Green qPCR SuperMix kit (TransGen Co.) on a Stratagene Mx3000P real-time PCR system (Stratagene, La Jolla, CA, USA) under the conditions of 94 °C for 30 s ...
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bioRxiv - Molecular Biology 2022Quote: ... All genes were analyzed with the SYBR green detection method (PCR Biosystems, UK) using the Stratagene Mx3000P real[time QPCR system (Agilent Technologies, Santa Clara, USA). Gene expression data were normalized against housekeeping genes (Gapdh in primary osteoblasts and Atp5b in bone tissue ...
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bioRxiv - Immunology 2022Quote: ... PCR amplification was performed on diluted lysis product (1:20 dilution) and 25 µl of PCR reaction mix according to the manufacturer instruction (Herculase II Fusion DNA Polymerase, Agilent Technologies, Les Ulis, France) using the following PCR primers ...
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bioRxiv - Developmental Biology 2019Quote: ... quantitative real time PCR was performed (PerfeCTa® SYBR® Green SuperMix for iQ™, Quanta Biosciences or Brilliant III Ultra Fast QPCR Master Mix, Agilent Technologies). Each sample was analysed in 3 biological replicates and each biological replicate was analysed in technical triplicates using primer pairs in Table S5.
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bioRxiv - Evolutionary Biology 2021Quote: Mutants in the VPg cistron were synthesized by long inverse site-directed PCR mutagenesis using the QuickChange II XL Kit (Stratagene, San Diego CA, USA) following the manufacturer’s instructions and using p35STunos as template ...
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bioRxiv - Biochemistry 2021Quote: ... were obtained by performing a directed evolution starting from the library described above and a random mutagenesis library constructed by error-prone PCR (Agilent, Santa Clara, CA, USA). We used a FRAD to perform directed evolution under identical conditions to our previous study36 ...
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bioRxiv - Immunology 2021Quote: ... The size of the amplicon was confirmed by analyzing 2 μl of PCR products using the Agilent D5000 ScreenTape System (Agilent D5000 ScreenTape, 5067-5588, Agilent D5000, Reagents 5067-5589). Products were quantified by analyzing 2 μl with the Quant-iT dsDNA High-Sensitivity Assay Kit (Thermo Fisher ...
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bioRxiv - Physiology 2023Quote: ... Standard curves for each gene were generated by cloning qPCR products into the pSCA vector with the Strataclone PCR cloning kit (Agilent, Santa Clara, CA, USA), isolating plasmid DNA using the GeneJET Plasmid Miniprep Kit (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... A mutant Acr library was made using ACX175 as a backbone and error-prone PCR with low mutation rate using GeneMorph II random mutagenesis kit (Agilent, Santa Clara, CA, USA). The resulting library was subcloned into a second plasmid containing AiEvo2 ...
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bioRxiv - Biochemistry 2023Quote: ... The cDNA of Affilin variant Af2p was used as template for error-prone PCR employing the GeneMorph II Random Mutagenesis Kit (Agilent, Santa Clara, CA, USA). The error rate was set to 10-14 mutations per kbp and the resulting theoretical library size was calculated to contain 6·1010 independent variants ...
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bioRxiv - Cell Biology 2020Quote: ... and pom1521-200-GFP (pKBB520) fusions were generated by amplifying sGFP from pJK19-1 using PCR with PfuUltra™ HF (Agilent, Inc. Santa Clara, CA) and primers that generated chimeric Pom152-sGFP PCR products for homologous recombination in yeast ...
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bioRxiv - Immunology 2020Quote: ... and Ii L17A was subsequently used as template for PCR quick change mutagenesis (all reagents used were included in the kit; QuickChange® Site-Directed Mutagenesis (Stratagene, La Jolla, CA, USA)) in order to generate the AP-3 binding motif RRP 21 ...
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bioRxiv - Microbiology 2024Quote: Quantification of SBW25 WT and ΔviscA was performed by qPCR targeting the mCherry gene using the AriaMx Real-Time PCR (Agilent Technologies, Santa Clara, CA, USA). The primers used are presented in Table 1 ...