Labshake search
Citations for Agilent :
1251 - 1300 of 1403 citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: Genes of interest were PCR cloned from either cDNA or gDNA of N2 Bristol strain using PfuUltra II Fusion HS DNA polymerase (Agilent). Amplicon was ligated into pSM vector backbone for expression in C ...
-
bioRxiv - Microbiology 2023Quote: ... The transformation reactions were plated on LB-carbenicillin agar plates and colonies screened by PCR using Herculase II Fusion Pfu DNA Polymerase (Agilent) followed by Sanger sequencing ...
-
bioRxiv - Cancer Biology 2023Quote: Mitochondrial DNA copy number was quantified by qPCR on total DNA from gastrocnemius muscle using MxPro Mx3000P real-time PCR Analyzer (Agilent technologies / Stratagene) and determining the mtDNA/nDNA ratio ...
-
bioRxiv - Neuroscience 2024Quote: ... The concentration of the genomic DNA was adjusted to 200 ng/µL before it was PCR amplified using Herculase II (Agilent). Approximately 400 ng purified PCR product (PCR purification kit ...
-
bioRxiv - Molecular Biology 2023Quote: ... The DNA obtained from cell cultures transfected with the RNP complex was amplified by nested PCR using Herculase II Fusion DNA Polymerase (Agilent). The first amplification was performed with primers IG36 and IG37 as follows ...
-
bioRxiv - Bioengineering 2023Quote: ... The library of CjCas9 variants was produced by random mutagenesis of the wild-type sequence using error-prone PCR approach (GeneMorph II kit from Agilent) (for primers ...
-
bioRxiv - Biochemistry 2023Quote: ... Site-directed mutagenesis was performed based on PCR of the full-length plasmid by using Pfu Turbo DNA polymerase (Stratagene). The entire cDNA was sequenced ...
-
bioRxiv - Cancer Biology 2023Quote: ... Number of pre-capture PCR cycles was 8 - 14 based on input DNA quantity and quality (assessed by Agilent TapeStation) as advised by the manufacturers’ protocol and run on an AB Veriti 96-well Thermocycler (Applied Biosystems ...
-
bioRxiv - Plant Biology 2024Quote: ... The synthesized cDNA was subsequently used as template for quantitative real time PCR based analysis of the expression levels of the target genes using Brilliant III Ultra-Fast SYBR QPCR MM (Agilent) in conjunction with Rotor-Gene® Q (Qiagen ...
-
bioRxiv - Biochemistry 2024Quote: ... PCR mixes were made up as follows in a total volume of 50 µL: 1X Pfu reaction buffer (Agilent, #600250), 0.2 mM dNTP (NEB® ...
-
bioRxiv - Molecular Biology 2024Quote: ... The PCR-amplified library was purified using Ampure XP beads and its quality was assessed on a Bioanalyzer 2100 system (Agilent). Diluted libraries were clustered on a pair-read flowcell and sequenced using a NovaSeq 6000 system (Illumina ...
-
bioRxiv - Genomics 2024Quote: ... each library underwent a reconditioning PCR to reduce heteroduplexes: libraries were concentrated down to 5 µl and mixed with 10 µl of Herculase Buffer (Agilent), 5 µl of 2.5 nM dNTPs ...
-
bioRxiv - Microbiology 2024Quote: ... The PCR amplicons were subsequently separated by Fragment Analyzer capillary electrophoresis using the DNF905 1-500 base pair kit (Agilent). Bands were quantified and PSI was calculated as follows ...
-
bioRxiv - Biophysics 2021Quote: ... The thermal variations were in the range 25-95°C in a stepwise increment of 1 °C/minute step and using a 96-well PCR plate (Agilent Technologies) sealed with optical quality sealing tape (Microseal® ‘B’ seal from BIO-RAD) ...
-
bioRxiv - Biochemistry 2021Quote: Impβ charge reversal point mutations were generated by a PCR-based protocol adapted from the QuikChange site-directed mutagenesis method (Agilent Technologies). Impβ mutant proteins were expressed and purified as the WT and their molecular masses verified by LC/ESI mass spectrometry (Table S5).
-
bioRxiv - Biochemistry 2021Quote: ... and Rev charge-reversal point mutations were generated by a PCR-based protocol adapted from the QuikChange site-directed mutagenesis method (Agilent Technologies). Rev mutant proteins were expressed and purified as the WT and their molecular masses verified by LC/ESI mass spectrometry (Table S5).
-
bioRxiv - Molecular Biology 2021Quote: ... the immunoprecipitated DNA and total DNA were quantified by Power SYBR Green PCR Master Mix in a Mx3000P Real-Time qPCR System (Agilent Technologies). The primers used for qPCR are listed in Sup ...
-
bioRxiv - Molecular Biology 2020Quote: ... Samples were purified with the QIAquick PCR Purification Kit and the final purified library was validated on a TapeStation (Agilent, G2991AA) and sequenced in 300 bp pair-end reads on an Illumina MiSeq sequencer at the Genomic Facility at the University of Guelph (Guelph ...
-
bioRxiv - Immunology 2021Quote: ... The amplified DNA purified and subsequently mutagenized by error-prone PCR (ePCR) via the GeneMorph II Random Mutagenesis Kit (Agilent Technologies) with a target nucleotide mutation frequency of 0-4.5 mutations/kb ...
-
bioRxiv - Genomics 2019Quote: ... fragments with UMIs were generated using 200 ng starting material (purified by ethanol precipitation) in 2 cycles of PCR with Herculase II Fusion DNA Polymerase (Agilent Technologies) using an equimolar mixture of P023poolseqNN-primers (1 mM final concentration ...
-
bioRxiv - Biochemistry 2019Quote: The genomic DNA template was amplified by the polymerase chain reaction (PCR) using Pfu Ultra High-Fidelity DNA polymerase (Agilent Technologies). Each 50 μL reaction consisted of genomic DNA (~12 ng) ...
-
bioRxiv - Genomics 2021Quote: ... All libraries were prepared in parallel with the pre-PCR purification steps carried out using a Bravo Workstation pipetting robot (Agilent Technologies).
-
bioRxiv - Systems Biology 2021Quote: ... 607 steady-state LC and 208 migrated LC from mastectomy skin were converted into ‘STAMPs’ for PCR library amplification (High Sensitivity DNA Assay, Agilent Bioanalyser) and tagmentation (Nextera XT ...
-
bioRxiv - Plant Biology 2021Quote: ... The L480P mutation in GL2 was generated by one-step PCR-based site-directed mutagenesis (Scott et al., 2002) using PfuUltra II Fusion HS DNA polymerase (Agilent Technologies) with primers listed in Supplemental Table 5 ...
-
bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR analysis of gene expression was undertaken using the KAPA SYBR FAST qPCR Kit and Stratagene Mx3005P qPCR System (Agilent Technologies). Thermal cycling conditions were as follows ...
-
bioRxiv - Systems Biology 2021Quote: ... 700 steady-state LC and ~300 migrated LC from mastectomy skin were converted into ‘STAMPs’ for PCR library amplification (High Sensitivity DNA Assay, Agilent Bioanalyser) and tagmentation (Nextera XT ...
-
bioRxiv - Microbiology 2019Quote: ... A site directed mutagenesis of the cloned UBP-1 PCR product in the TOPO 2.1 vector was carried out using a QuikChange® multi-site directed mutagenesis kit (Agilent technologies) using the following primer combinations ...
-
bioRxiv - Microbiology 2020Quote: ... we carried out site direct mutagenesis of pCR-ompT-GFP according to the Quikchange II site-directed mutagenesis kit (Agilent Technologies).
-
bioRxiv - Genomics 2021Quote: ... All libraries were prepared in parallel with the pre-PCR purification steps carried out using a Bravo Workstation pipetting robot (Agilent Technologies). 9-12 libraries were sequenced as a multiplex on one Illumina HiSeq 2000 lane using 125bp paired-end read length.
-
bioRxiv - Cancer Biology 2020Quote: ... QRT-PCR was performed in triplicate including a non-template control using the Mx3000P system (Agilent Technologies, Inc., Santa Clara, CA). Oligos (Invitrogen ...
-
bioRxiv - Cancer Biology 2022Quote: ... All cell lines were tested for mycoplasma at the beginning of the studies (tested negative with MycoSensor PCR Assay Kit, Agilent, #302109) and have never exhibited contamination symptoms after initial testing.
-
bioRxiv - Plant Biology 2022Quote: ... and gl2Amo mutants were generated by one-step PCR-based site-directed mutagenesis (Scott et al., 2002) using PfuUltra II Fusion HS DNA polymerase (Agilent Technologies). The Phusion® Site-Directed Mutagenesis Kit (Thermofisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... full-length cDNAs of the zebrafish banp gene were amplified with Polymerase Chain Reaction (PCR) and sub-cloned into pBluescript II SK(+) vectors (Stratagene/Agilent Technologies). cDNA fragments were amplified from mRNA with PCR using the primers for cenpt ...
-
bioRxiv - Cell Biology 2022Quote: ... 0.0025 μl of a 1:40,000 diluted ERCC spike-in concentration stock and all cDNA was amplified with 22 PCR cycles before QC control with a Bioanalyzer (Agilent Biosystems). The libraries were sequenced on a HiSeq2500 at the National Genomics Infrastructure (NGI) ...
-
bioRxiv - Plant Biology 2021Quote: ... Quantitative real-time PCR was performed with Thunderbird SYBR qPCR mix (Toyobo) in Stratagene MX3000P real-time qPCR system (Agilent Technologies). The transcript levels were calculated with standard curve method and normalized by that of internal control PROTEIN PHOSPHATASE 2A-3 (PP2A3)69 ...
-
bioRxiv - Molecular Biology 2020Quote: ... The qPCR assays were carried out in a MX 3005 real-time PCR system (Agilent, Santa Clara, CA 95051, United States) using Brilliant III Ultra-Fast SYBR Green qPCR Master Mix (Agilent) ...
-
bioRxiv - Immunology 2020Quote: ... The recombinant Ad5 genomes with HA inserts were linearized with PacI and buffer exchanged using a Strataprep PCR purification kit (Agilent Technologies). The linearized recombinant gDNA was transfected into 293 cells using the PolyFect Transfection Reagent (Qiagen) ...
-
bioRxiv - Bioengineering 2021Quote: The integrated sgRNA library was PCR amplified and separately barcoded for each collected population with Herculase II Fusion DNA Polymerase (Agilent 600677). Samples were then pooled and sequenced on an Illumina NextSeq flow cell with aligned read counts as follows for each screen:
-
bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed with 4 μl Brilliant III Ultra-Fast SYBR QPCR as instructed by the manufacturer (Agilent Santa Clara). All primers for quantification of either expression of viral m/cRNAs or antiviral responses are shown below.
-
bioRxiv - Pathology 2019Quote: ... Pathogenic mutations and variations in the INF2 sequence were introduced by a PCR-based mutagenesis (Cat No: 200523, QuickChange II Site-Directed Mutagenesis kit, Agilent Technologies). To achieve stable expression of fragments in human podocytes ...
-
bioRxiv - Cell Biology 2019Quote: ... hFN10 containing a TAG stop codon at position 1493 was generated by PCR-based mutagenesis with the Quick-change kit (Agilent Technologies), cloned into bacterial expression plasmid pET11a and verified by DNA sequencing ...
-
bioRxiv - Molecular Biology 2019Quote: ... DNA obtained from the three PCR reactions were then combined and analyzed for size on a High Sensitivity DNA chip (Agilent Technologies) and sequenced as SE100 ...
-
bioRxiv - Biochemistry 2019Quote: ... carrying the human WT RAD51 (WT-pET11d) was used as a template for the PCR-mediated QuikChange site-directed mutagenesis (Agilent Technologies) to introduce F86E substitution (F86E-pET11d ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The error-prone library of the initial hit of the ACE2 helix scaffolded design was constructed by error-prone PCR with a GeneMorph II Random Mutagenesis Kit (Agilent Technologies) with manufacturer’s instructions ...
-
bioRxiv - Biophysics 2020Quote: ... The VDAC1 mutations at serine 215 to glutamate (S215E) was generated by PCR with QuikChange site-directed mutagenesis kit (Agilent Technology) using pET-VDAC1 as cDNA templates and the following primers ...
-
bioRxiv - Microbiology 2020Quote: ... Abundances of bacterial and archaeal amoA genes and bacterial 16S rRNA genes were determined by quantitative PCR using a Mx3000P instrument (Agilent, CA). Primer combinations were AmoA-1F/ AmoA2R (Rotthauwe et al. ...
-
bioRxiv - Immunology 2021Quote: ... PCR amplification verification and library validation was done using a TapeStation electrophoresis system and D1000 ScreenTape reagents (Agilent, Santa Clara, California). Libraries were quantified using a Qubit 4 Fluorometer and the Qubit dsDNA HS assay kit (ThermoFisher Scientific ...
-
bioRxiv - Genomics 2021Quote: ... RT qPCR analysis was run for the samples using a miScript SYBR Green PCR Kit (Cat. No. 218073) on a Stratagene Mx3005P (Agilent Technologies). Housekeeping gene GAPDH primers were purchased from Qiagen (Cat ...
-
bioRxiv - Genomics 2021Quote: ... cDNA were amplified by 10 PCR cycles and purified before assessing their quality on the bioanalyzer (2100 high sensitivity DNA assay, Agilent Technologies). 150 pg of cDNA were used as input to prepare the libraries using the Nextera XT DNA kit (Illumina) ...
-
bioRxiv - Neuroscience 2020Quote: ... Human α7-nAChR R208I-GFP and E211N-GFP were generated from pcDNA3.1-CHRNA7-mGFP and human α3-nAChR I284R-GFP and N287E-GFP were generated from human α3-nAChR-GFP by PCR based QuikChange® Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s protocol ...