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Citations for Agilent :
1251 - 1300 of 1341 citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The ligation product was enriched with 15 PCR cycles and the final library was validated on an Agilent 2100 Bioanalyzer (Agilent Technologies) using the DNA 7500 kit (Agilent Technologies).
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bioRxiv - Cell Biology 2022Quote: ... All cell lines tested negative for mycoplasma using the Mycoplasma Plus™ PCR kit (#302008, Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Immunology 2022Quote: ... The resulting cDNA (equivalent to 500ng of total RNA) was amplified using the SYBR Green real-time PCR kit and detected on a Stratagene Mx3005 P (Agilent Technologies). qPCR was conducted using forward and reverse primers (sequences available upon request) ...
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bioRxiv - Microbiology 2022Quote: ... the gentamicin resistance marker (PflaB-aaC1) was amplified from pMH105 (20) by PCR and then TA-cloned into pSC-A-amp/kan (Agilent Technologies). Following confirmation of the TA clones ...
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bioRxiv - Microbiology 2023Quote: ... BST2 M1A) and ATG5 (ATG5-K130R) were made by PCR mutagenesis using the QuikChange II XL site directed mutagenesis kit (Stratagene, France). The cDNA encoded ATG5-K130R was cloned in frame with GFP tag into the pEGFP-C1 vector ...
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bioRxiv - Microbiology 2022Quote: ... The Agilent High Sensitivity DNA Kit for naturally contaminated shellfish was used to visualise second-round PCR products for Bioanalyzer 2100 (Agilent Technologies).
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bioRxiv - Neuroscience 2023Quote: ... All point mutations of plasmids were generated by Quick Change PCR mutagenesis using Pfu Ultra polymerase (Agilent Technologies, Santa Clara, CA).
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bioRxiv - Molecular Biology 2023Quote: ... The T94D mutation was introduced in the plasmids by PCR using the QuickChange Multi-Site Directed Mutagenesis kit (#200514 -Agilent Technologies). The sequence of the primer used to introduce the mutation T94D in the Nme1 sequence of plasmids is reported in key resources table ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time quantitative PCR reactions using SYBRgreen fluorescent reagent and/or EvaGreen™ were achieved on the Stratagene Mx3000P system (Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Flag-tagged wt and Ex19Del human EGFR constructs in the lentiviral VIRSP vector were generated by PCR with Herculase II Fusion DNA Polymerase (Agilent technologies) from EGFR WT (a gift from Matthew Meyerson ...
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bioRxiv - Biochemistry 2023Quote: ... The mutants in the present study were generated through use of SOE-PCR (as described earlier)19 or through use of the QuikChange® Site-Directed Mutagenesis Kit from Stratagene. Sequences of DNA primers used for PfuAmyGT and the point mutants are mentioned in Supplementary Information Table 1 (ST1).
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... The putative ESRRA binding site 1 was mutated using PCR-based site-directed mutagenesis and QuikChange Site-Directed Mutagenesis Kit (Stratagene #200518) to obtain mutated constructs pGL3-Leptin mut 1-luc.
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bioRxiv - Molecular Biology 2023Quote: HEK293T cells were obtained from ATCC (CRL-3216) and verified to be free from mycoplasma contamination using the MycoSensor PCR Assay Kit (Agilent Technologies). Cells were cultured as in DMEM (Lonza ...
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bioRxiv - Genomics 2023Quote: ... PCR amplified libraries were purified from 8% PAGE gels and subsequently analysed with the Agilent High Sensitivity DNA assay (Agilent, Bioanalyzer).
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bioRxiv - Biochemistry 2023Quote: ... Mutations were introduced into the generated pE-SUMO-α-actinin-2 ABD through site-directed PCR mutagenesis (PfuUltra High-Fidelity DNA Polymerase, Agilent). The following primers were used to introduce cardiomyopathy mutations into the α-actinin-2 ABD sequence.
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bioRxiv - Molecular Biology 2023Quote: ... Real-time PCR analysis was performed using Perfect Start Green qPCR Super Mix (+DyeII) (AQ602-24, TransGen, Beijing, China) and the AriaMx Real-Time PCR System (Agilent Technologies). See Table S2 for primer information.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was used to synthesize cDNA and quantification of viral gene expression was performed on the Mx3005P Real-Time PCR System (Stratagene, USA), using Ultra SYBR Mixture (Low ROX ...
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bioRxiv - Immunology 2024Quote: ... 11 cycles of TCR target enrichment PCR 1 and 2 were performed and the resulting cDNA was quantified on an Agilent Bioanalyzer High Sensitivity chip (Agilent Technologies). TCR libraries were prepared and indexed with 9 cycles of amplification using the PN-220103 Chromium i7 Sample Index Plate ...
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bioRxiv - Cancer Biology 2023Quote: ... 5ng of cDNA was used for the qRT-PCR reaction using Brilliant III Ultrafast SYBR Green QPCR master mix (Agilent Technologies) on AriaMx qRT-PCR system (Agilent Technologies) ...
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bioRxiv - Plant Biology 2024Quote: ... was carried out in a 25 μL reaction volume using the Brilliant II SYBR Green PCR Master Mix (Stratagene, Agilent technologies). Primer concentrations were set at 0.2 μM ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR amplification of the eluted DNA was performed as described in the manufacturer’s protocol but with the addition of 2.5 µl Evagreen (20X) for visualization of the amplification curves on an AriaMx Real-time PCR system (Agilent Aria 1.7). The denaturation and annealing/extension steps of the PCR amplification were performed for around 12 cycles and stopped before the curves plateaued ...
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bioRxiv - Microbiology 2024Quote: ... 40x 10 sec 95°C and 30 sec 60°C with the Brilliant III Ultra-Fast qRT-PCR master mix (Agilent, 600884) were used according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR reactions employing SYBRgreen fluorescent reagent and/or EvaGreen™ were performed in the Stratagene Mx3000P system (Agilent Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... 1 µL of eluted RNA and 5 µL RNA ScreenTape sample buffer was mixed in an 8-well PCR tube strip (Agilent Technologies) via vortexing for one minute (IKA MS3 Vortexer ...
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bioRxiv - Cell Biology 2024Quote: ... All the mutants described in this work were generated via PCR mutagenesis using cloned PFU polymerase (Agilent Technologies, Santa Clara, CA).
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bioRxiv - Cancer Biology 2024Quote: ... using miR30-adapted sequences (5-6 shRNAs per target) by PCR-cloning a pool of oligonucleotides synthesized on 55k customized arrays (Agilent Technologies) using a well-established system 84–87 ...
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bioRxiv - Cell Biology 2024Quote: HEK293T cells were obtained from ATCC (CRL-3216) and verified to be free from mycoplasma contamination using the MycoSensor PCR Assay Kit (Agilent Technologies). Cells were cultured as in DMEM (Lonza ...
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bioRxiv - Molecular Biology 2020Quote: ... the purified products were cloned using the dual-colour selection Strataclone PCR cloning kit according to the manufacturer’s protocol (Stratagene, La Jolla, USA). Up to eight colonies containing the PCR product were selected and inoculated on Luria Bertani agar slants ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR reaction was performed according to the manufacturer’s protocol of Cloned Pfu DNA polymerase Taq (Agilent Technology, Santa Clara, CA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... The cDNA was diluted (1:10-20) and used for qRT-PCR in technical triplicate using Brilliant III SYBR master mix (Agilent Technologies, 600882) and CFX384 Touch Real-Time PCR Detection Systems (BioRad) ...
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bioRxiv - Cell Biology 2020Quote: ... These DN proteins were generated by PCR-based mutagenesis using the QuikChange Lightning Multi Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3.1(+ ...
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bioRxiv - Cell Biology 2020Quote: pcDNA3 + mNICD W1758A: this plasmid was generated by PCR-based mutagenesis using the QuikChange Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3 + mNICD as a template and the primers W1758A F and W1758A R.
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bioRxiv - Biochemistry 2019Quote: ... 1 μl of each PCR product was subjected to Chip-based capillary electrophoresis with the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA) and the proportional amount of each fragment to the whole yield of splicing products was calculated ...
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bioRxiv - Bioengineering 2020Quote: ... LEU4 and Ll_ilvD were generated by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA). The CEN plasmids harboring wild-type ILV6 (pYZ127) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 μL of the cDNA was used as template with the Thermo Scientific DyNAmo Flash SYBR Green PCR Master Mix in a Mx3005P QPCR System (Agilent Technologies, USA). A 2 step qPCR protocol was used ...
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bioRxiv - Microbiology 2020Quote: ... Lens was introduced to the leader region of the MC-II through MC-VI and MC-V-dotO arrays in our donor plasmids by quickchange PCR with Pfu Turbo Polymerase (Agilent #600250-52). boxA(−58 ...
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed using SYBR® Green Premix Pro Taq HS qPCR Kit (Accurate biology, China) on an AriaMx Real-time quantitative PCR machine (Agilent, USA). The PCR reaction conditions were 95°C for 30 s ...
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bioRxiv - Cell Biology 2022Quote: ... All other constructs were made by PCR amplification followed by standard restriction enzyme cloning or by site-directed mutagenesis kit (QuickChange II, Agilent Technologies, 200523). All oligonucleotides were obtained from Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... full-length cDNAs of the zebrafish banp gene were amplified with Polymerase Chain Reaction (PCR) and sub-cloned into pBluescript II SK(+) vectors (Stratagene/Agilent Technologies). cDNA fragments were amplified from mRNA with PCR using the primers for cenpt ...
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bioRxiv - Cancer Biology 2022Quote: ... by polymerase chain reaction (PCR). Ki-67 (Catalog no. M7240) and CD4 (Catalog no. M731029-2) antibodies were purchased from Dako (CA, USA). FOXO3a (Catalog no ...
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bioRxiv - Molecular Biology 2019Quote: ... The original eGFP-C2-DEF6 plasmid (5) was used to generate the mutant DEF6 proteins by mutagenesis PCR (QuikChange Lightning Multi Site-Directed Mutagenesis Kit, Agilent Technologies, #210516). All mutations were verified by sequence analysis (see Suppl ...
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bioRxiv - Immunology 2019Quote: ... qRT-PCR reactions were made for selected genes using the Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent Technologies) as previously described.
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bioRxiv - Immunology 2019Quote: ... qRT-PCR reactions were made for selected genes using the Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent Technologies) as previously described.
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bioRxiv - Genomics 2019Quote: ... Gene expression was performed on 7900 HT fast real time PCR system (Applied Biosystem, USA) using 2× Brilliant III SYBR@ Green qPCR Master Mix (Agilent Technologies, USA) and gene specific primers (Supplementary Table 1) ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Finally ...
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bioRxiv - Biochemistry 2022Quote: ... The phPAI-1 library was generated by error prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) with primers that maintain the AscI and NotI restriction sites (SI Table 1) ...
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bioRxiv - Molecular Biology 2022Quote: ... Adapter dilution (1:4) and PCR amplification (26 cycles) were optimized in a pilot experiment using Bioanalyzer DNA1000 (Agilent, Sant Clara, USA) chips as a read out of library size and quantity ...
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bioRxiv - Molecular Biology 2022Quote: ... The purity and integrity of the PCR products were analysed on an Agilent 2100 Bioanalyzer following manufacturer recommendations for high sensitivity DNA chips (Agilent 5067-4626). Sequencing libraries were prepared using Nextera DNA Library Prep Kit (Illumina FC-131-1024 ...
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bioRxiv - Physiology 2023Quote: ... Mutations were introduced by PCR-based site-directed mutagenesis method using the Quick Change II XL Site-Directed Mutagenesis Kit (Agilent Technologies, USA) and verified by sequencing (Supplementary Table 2).
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bioRxiv - Immunology 2023Quote: ... The analysis of the PCR products was performed using the LabChip electrophoretic Agilent 2100 Bioanalyzer microchip electrophoresis system (Agilent Technologies, Wokingham, UK). Briefly ...