Labshake search
Citations for Agilent :
1001 - 1050 of 7234 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... pcDNA3.1zeo+/ nFlag-cmScarlet-hRobo1 was generated by inserting the Flag tag sequence at the N-terminus after the signal peptide sequence of Robo1 with QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies ...
-
bioRxiv - Immunology 2024Quote: ... residues D141 of Regnase-1 and D252 of Regnase-3 were mutated to asparigines using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) following manufacturer’s protocol ...
-
bioRxiv - Genetics 2024Quote: Membranes were hybridized with “probe 4” for the HIS4::LEU2 hotspot 100 which was 32P-dCTP labelled using the Prime-It II Random Primer Labeling Kit (Stratagene). Following hybridization ...
-
bioRxiv - Microbiology 2024Quote: ... The plasmids expressing the BA.5 spike with the A570S mutation or the A570S/K147E double mutation were obtained by site-directed mutagenesis using a QuikChange II Site-Directed Mutagenesis Kit (Agilent). The dual split protein (DSP ...
-
bioRxiv - Cancer Biology 2024Quote: ... four sequential site-directed mutagenesis reactions were performed following the QuikChange II XL Site-Directed Mutagenesis Kit protocol (Agilent, 200521). The following primers were generated using Agilent’s QuikChange Primer Design tool ...
-
bioRxiv - Neuroscience 2022Quote: ... Heat retrieval was performed with the Envision Flex Tris low pH buffer (Agilent) 30 min at 97°C on board ...
-
bioRxiv - Plant Biology 2024Quote: ... The analysis was performed by a low-resolution mass spectrometer (Agilent model 5977B) with quad-coupled analyser coupled to a gas chromatograph (Agilent model 7890B) ...
-
bioRxiv - Microbiology 2022Quote: ... The MHC-II binding site mutations were introduced into pKK33 by site-directed mutagenesis (QuickChange II, Agilent Technologies) using the primer set SECF44A/L45Afor/ SECF44A/L45Arev ...
-
bioRxiv - Immunology 2021Quote: ... mouse anti-human CD3 (Dako) and mouse anti-HIV-1 P24 (Dako) ...
-
bioRxiv - Immunology 2021Quote: ... FITC anti-human CD44 (DAKO), Purified NA/LE mouse anti-human CD253 (BD Biosciences) ...
-
bioRxiv - Microbiology 2020Quote: Universal Human Reference RNA (Stratagene) was treated with RNase-Free DNase Set (Qiagen ...
-
bioRxiv - Cancer Biology 2023Quote: ... mouse anti-human CD8 (DAKO), and mouse anti-human TAG72 (AB16838 ...
-
bioRxiv - Cancer Biology 2023Quote: ... mouse anti-human CD3 (DAKO), mouse anti-human CD4 (DAKO) ...
-
bioRxiv - Cancer Biology 2023Quote: ... mouse anti-human CD4 (DAKO), mouse anti-human CD8 (DAKO) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Human universal total RNA (Agilent) was used as a positive control ...
-
bioRxiv - Immunology 2023Quote: ... For human CD45 (Dako, M0701), CD19 (Abcam ...
-
bioRxiv - Genetics 2019Quote: ... DNA libraries were prepared by using the SureSelect Human All Exon V6 kit (Agilent Technologies, Santa Clara, CA, USA) and were paired-end sequenced on the Illumina HiSeq 4000 platform at 100X coverage ...
-
bioRxiv - Neuroscience 2021Quote: ... The mutations were introduced into human APP695 using the QuickChange Site-Directed Mutagenesis Kit (Stratagene, San Diego, CA, U.S.) and confirmed by sequencing ...
-
bioRxiv - Pathology 2022Quote: ... DNA microarray analysis was performed using a Quick Amp labeling kit and a Whole Human Genome DNA Microarray 4×44K according to the manufacturer’s protocol (Agilent). Signal intensity was normalized by adjusting the data to a 75th percentile value ...
-
bioRxiv - Genetics 2020Quote: ... enrichment and elution were performed according to manufacturer’s instructions and protocols (SureSelect Human All Exon Kits Version CRE, Agilent). The library was sequenced on an Illumina HiSEQ 2500 as paired-end 75bp reads ...
-
bioRxiv - Cell Biology 2023Quote: Single point mutations were inserted in the human RAB6A sequence at position 156 using the QuickChange mutagenesis kit (Agilent).
-
bioRxiv - Developmental Biology 2019Quote: ... RT-PCR was performed to amplify the dazl coding region using Easy-A High Fidelity Taq polymerase (600400, Agilent) (primers in Supplemental Table 1) ...
-
bioRxiv - Plant Biology 2021Quote: ... The genomic SPL7 (AT5G18830.1) coding region (translational start to stop codon) was PCR-amplified and cloned into the pBluescript SK+ vector (Stratagene/Agilent Technologies ...
-
bioRxiv - Genetics 2022Quote: ... for fragments corresponding to a 10.3-kb genomic region (coordinates 66,234 – 76,516) of the BL21AI genome [14] using custom-designed biotinylated ssDNA probes (Agilent SureSelect) and streptavidin-bead purification ...
-
bioRxiv - Neuroscience 2021Quote: ... A negative control construct in which the start codon was mutated to an ATT was generated using a Quikchange II Site-directed mutagenesis kit (Agilent 200521). The wild-type and mutant plasmids were verified by Sanger sequencing ...
-
bioRxiv - Microbiology 2020Quote: ... The SARS-CoV-2 RBD constructs carrying point mutation were generated by following the standard protocol from QuikChange® II XL Kit (Agilent). The cloned genes were sequenced to confirm that no errors had accumulated during the PCR process ...
-
bioRxiv - Cell Biology 2019Quote: The PCR product was inserted in pEGFP-N1 vector using Gibson cloning (Gibson et al., 2009) and mutagenized using the Quickchange II site directed mutagenesis kit (Agilent #200523) and the following primer set:
-
bioRxiv - Evolutionary Biology 2020Quote: ... Point mutations were inserted by using the Quick-change II kit for site directed mutagenesis (Agilent Biosciences, Santa Clara, CA, USA), TOPO-blaOXA-48 as a template and the respective primers (Table S3) ...
-
bioRxiv - Neuroscience 2021Quote: The GFP tagged FUS P525L variant was created by site directed mutagenesis on the GFP tagged FUS WT construct using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent; 200521). The primers used to generate the construct were ...
-
bioRxiv - Molecular Biology 2021Quote: ... To introduce the g.26652G>A mutation we used the Quick-change II XL Site-directed Mutagenesis Kit (Agilent Technologies, USA), according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... The amplified DNA purified and subsequently mutagenized by error-prone PCR (ePCR) via the GeneMorph II Random Mutagenesis Kit (Agilent Technologies) with a target nucleotide mutation frequency of 0-4.5 mutations/kb ...
-
bioRxiv - Developmental Biology 2019Quote: ... truncated and deletion versions of RAB13 3’UTR were generated by PCR using 0.3 μM sequence-specific primers (Extended Data Table 2) and Platinum Pfx DNA polymerase or using QuikChange II Site-Directed Mutagenesis kit (Agilent Technologies) following the manufacturer’s instructions and introduced into the pcDNA3-HBB-24XMS2SL-MCS using the NheI and XhoI sites.
-
bioRxiv - Biochemistry 2021Quote: ... and PcrA V448C were generated by site-directed mutagenesis of the wild type construct using the Quikchange II XL kit (Agilent Technologies). In the last gel filtration chromatography of PcrA V448C ...
-
bioRxiv - Genomics 2021Quote: ... Gene-specific probes were generated by random priming in the presence of ATP [α32P] using the Prime-It II Random Primer Labeling Kit (Agilent, 300385) using PCR generated DNA template produced from gDNA isolated from a wild type S.pombe strain (YP71 ...
-
bioRxiv - Cell Biology 2020Quote: ... To construct a plasmid encoding GAS6 tagged with Myc-His (pSecTag2-hGAS6-MycHis) the stop codon from pSecTag2-hGAS6 was removed using the QuickChange II XL Site-Directed Mutagenesis kit (Agilent Technologies). Next ...
-
bioRxiv - Microbiology 2021Quote: Mutations in AdMarRars were introduced by site-directed mutagenesis using QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). The mutagenic oligonucleotides used for both strands and the respective changes introduced (underlined ...
-
bioRxiv - Bioengineering 2020Quote: ... respectively in ThuE were designed by site-directed mutations in optical sucrose sensors by using the QuickChange II Site-Directed Mutagenesis (SDM) Kit (Agilent Technologies) following the manufacturer’s instructions ...
-
bioRxiv - Physiology 2021Quote: Two truncated recombinant fibrinogen α-chain variants (α220 and α390) were produced using QuikChange II Site-Directed Mutagenesis Kits from Agilent Technologies (Stockport ...
-
bioRxiv - Physiology 2020Quote: ... by site-directed mutagenesis of a pCI-NaV1.5 plasmid containing the human NaV1.5 hH1C cDNA (30) (NCBI Reference Sequence NM_000335) using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent, Sant Clara, CA) or the Q5 Site-Directed Mutagenesis kit (New England Biolabs ...
-
bioRxiv - Plant Biology 2021Quote: ... CA- and DN-RABG3 variants were generated by site directed mutagenesis with QuickChange Lightning II Site-Directed Mutagenesis Kit (Agilent Technologies) according to manufacturers’ instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... two arginines at position 682 and 683 were changed to alanines using the QuickChange II XL site-directed mutagenesis kit (Agilent Technologies) and the plasmid pIB-spike-V5 as a template ...
-
bioRxiv - Cell Biology 2021Quote: ... A gene fragment encoding three copies of the 9-amino-acid HA epitope followed by EcoR1 and XbaI restriction sites was inserted using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent technologies). The resulting GSPK-HA transgene plasmid (13,468 bp ...
-
bioRxiv - Cell Biology 2021Quote: ... We performed mutagenesis with the primers (Table S1) designed for the R397W mutation according to the manufacturer’s instruction (QuikChange II Site-Directed Mutagenesis Kit, Agilent Technologies, USA). Upon reaching 50-60% density ...
-
bioRxiv - Cell Biology 2021Quote: Site-directed mutations of the phosphorylation sites were created using similar methods as the Quickchange® II XL kit from Agilent technologies by using the primers listed below to recreate the whole vector via PCR with the new mutation in place ...
-
bioRxiv - Cancer Biology 2019Quote: ... The SfiI site in mouse Tns4 cDNA was mutated using the QuikChange II XL mutagenesis kit (Agilent, Santa Clara, CA, USA) and the primers listed in Supplementary Table 1 ...
-
bioRxiv - Cell Biology 2019Quote: ... or turboGFP-MCMBPΔNLS (bipartite NLS was identified using cNLS Mapper35 generated by site directed QuickChange II XL Site-Directed Mutagenesis kit (Agilent, 200522) with primers (forward ...
-
bioRxiv - Microbiology 2020Quote: ... we carried out site direct mutagenesis of pCR-ompT-GFP according to the Quikchange II site-directed mutagenesis kit (Agilent Technologies).
-
bioRxiv - Biophysics 2021Quote: The mutant A350P mutant was generated using pET-LA as template by site directed mutagenesis (QuickChange II XL Site Directed Mutagenesis Kit, Agilent-USA). The primers used for the same were a ...
-
bioRxiv - Biophysics 2020Quote: ... The desired base pairs coding for Cys were introduced using overlapping primers with the QuikChange II site-directed mutagenesis kit (Agilent Technologies) and were confirmed by DNA sequencing (GENEWIZ ...
-
bioRxiv - Molecular Biology 2022Quote: ... we created pcDNA3-AhR-1(LBD)-VP16 by site-directed mutagenesis of the pcDNA3-AhR-1-VP16 plasmid with the QuikChange II Site-Directed Mutagenesis Kit (Agilent, 200523). The following primer pair was used to create an L to A substitution at L363 ...