Labshake search
Citations for Agilent :
5401 - 5450 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... RNA Integrity Number (RIN) and DV200 were determined by BioAnalyzer electrophoresis using an RNA 6000 Pico Kit (#5067-1513, Agilent). The DV200 metric refers to the percentage of total profiled RNA fragments greater than 200bp in length ...
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bioRxiv - Systems Biology 2022Quote: ... Adherent NSCs were infected with retro-Cre-Tomato retrovirus (5μl of virus was used to infect 3x105 cells) or nucleofected with expression constructs according to instructions of the Amaxa nucleofection kit (Agilent) [28] ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Amplified and purified sequencing libraries were analyzed on an Agilent 2100 Bioanalyzer (High sensitivity DNA analysis kit, Agilent, 5067-4626), pooled in equimolar amounts (2.47 ng/μL) ...
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bioRxiv - Molecular Biology 2022Quote: ... Point mutations in the repM gene were generated using specific primers and a QuikChange Site-Directed Mutagenesis kit according to the protocol supplied by the manufacturer (Stratagene). All oligonucleotide primers used in this study are listed in Supplementary Table S2.
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bioRxiv - Cancer Biology 2023Quote: ... The stranded mRNA libraries were analyzed on a 2100 Bioanalyzer instrument following the manufacturer’s protocol “Agilent DNA 7500 kit” (G2938-90024, Agilent Technologies), diluted to 10nM and pooled equimolar into multiplex sequencing pools for paired end sequencing on the NovaSeq 6000 Illumina sequencing instrument ...
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bioRxiv - Immunology 2023Quote: ... All mutations in this study were introduced using the QuikChange II XL site-directed mutagenesis kit (Agilent Technologies, Cat #200521), KLD Enzyme Mix ...
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bioRxiv - Immunology 2022Quote: ... SARS-COV-2-Strunc variants were generated in house by site-directed mutagenesis (QuikChange Multi Site-Directed Mutagenesis Kit, Agilent) starting from synthetic DNA (Genscript) ...
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bioRxiv - Molecular Biology 2023Quote: ... and C66A triple mutation), KR5>A (contains K286A, R287A, K288A, K300A, and R301A mutations) were done using QuikChange Mutagenesis Kit (Agilent). Deletion mutants (1-280 ...
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bioRxiv - Microbiology 2023Quote: ... The resulting pHD1344tub(PAC)-mt-LAF3-Cterm3V5 plasmid was then used as a template for site-directed mutagenesis of D211 (QuikChange II kit; Agilent) using forward and reverse primers listed in Supplemental Table 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged human SLFN14 D249A was generated by site directed mutagenesis with the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) using reverse primer SS1 (5’-atccaccccaatgaggacatatcc-3’ ...
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bioRxiv - Genomics 2023Quote: XF Cell Mito and XF Glycolysis Stress Test Kits were used to measure mitochondrial and glycolytic function on a Seahorse Extracellular Flux Analyzer (Agilent). The Seahorse microplate was coated with Cultrex (R&D Systems ...
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bioRxiv - Neuroscience 2023Quote: ... and their quality was assessed by capillary electrophoresis on the FragmentAnalyzer using the NGS High Sensitivity kit (DNF-474, Agilent). The libraries were pooled and sequenced on an Illumina NovaSeq 2000 ...
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bioRxiv - Microbiology 2023Quote: ... and a barcoding primer (unique for each sample to allow for demultiplexing of reads) with the Easy A cloning kit (Agilent). A 200–400 bp product was gel-purified and sequenced on an Illumina NextSeq Platform (in lab ...
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bioRxiv - Microbiology 2023Quote: ... This DNA was then subject to an initial round of PCR amplification with primers oTB535 and oTB536 with the Easy A cloning kit (Agilent), digested with NotI (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfer of the Gateway cassette from these two entry vectors to the pBIFCt-2in1-NN destination vector 57 was performed through LR clonase II kit (Agilent).
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bioRxiv - Biochemistry 2022Quote: ... The quality of total RNA was examined using a fragment analyzer system with an Agilent HS RNA kit (Agilent Technologies). Libraries were prepared using an MGIEasy RNA Directional Library Prep Set (MGI Tech Co. ...
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bioRxiv - Microbiology 2023Quote: ... and pKTP109 (catR, Para::ftsI) by using the QuickChange site-directed mutagenesis kit according to the manufacturer’s instruction (Agilent Technologies). The list of primers used in this study was previously published.
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bioRxiv - Developmental Biology 2023Quote: ... RNA concentration and quality of the RNAseq libraries were assessed using a 2100 Bioanalyzer with the High Sensitivity DNA Kit (Agilent), and libraries were sequenced as single-end 50 bp reads on an Illumina HiSeq 2500.
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bioRxiv - Neuroscience 2023Quote: ... RNA quality of the tissue sections (RIN > 7) was confirmed using the Agilent RNA 6000 Nano Kit on the Bioanalyzer 2100 system (Agilent). Visium spatial gene expression slides were processed according to manufacturer instructions (10X Genomics ...
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bioRxiv - Cell Biology 2023Quote: ... vector containing the SARS-CoV-2 HA-ORF3a gene was used in site-directed mutagenesis using a QuikChange II site-directed mutagenesis kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA integrity was validated on a 2100 Bioanalyser with the Agilent RNA 6000 Nano kit (Agilent Technologies, Santa Clara, USA). Sequencing libraries were generated with the Illumina TruSeq RNA library Prep kit v2 (StarSEQ ...
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bioRxiv - Cell Biology 2023Quote: ... Site-directed mutagenesis for Drp1 R456E and Y493A was completed to introduce each mutation into this construct individually using the QuikChange Lightning kit (Agilent) with primers from Integrated DNA Technologies (Coralville ...
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bioRxiv - Biophysics 2023Quote: ... derivative encoding two cysteine residues at positions 366 and 511 [constructed from plasmid pGEMD (-Cys)46 by use of site-directed mutagenesis (QuikChange Site-Directed Mutagenesis Kit; Agilent) to replace codons 366 and 511 by a codon encoding cysteine residue] ...
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bioRxiv - Cell Biology 2023Quote: ... the pK2:Nβ-GFP construct was used as template for site-directed mutagenesis by PCR using the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) following the manufacturer’s instructions with the concomitant mutagenic primers for each target base substitution (S2 Table) ...
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bioRxiv - Cell Biology 2023Quote: ... The total RNA quality and quantity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0 ...
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bioRxiv - Microbiology 2023Quote: ... The mpox sA28 and sH2 expression plasmids were generated from the above vaccinia sA28 and sH2 constructs by site-directed mutagenesis using the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent). All the vaccinia A28 mutant constructs ...
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bioRxiv - Molecular Biology 2023Quote: ... The small amplicon manufacturer’s instructions were followed for the library kit and completed libraries were quantified and verified on the Tapestation 4200 instrument (Agilent). The completed libraries were diluted to 4 nM ...
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bioRxiv - Microbiology 2023Quote: The substitution of amino acids via SDM was carried out using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The quality of the amplified and bead-cleaned-up libraries was determined using a 5300 fragment analyzer with the standard sensitivity NGS kit (Agilent). Libraries were then pooled ...
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bioRxiv - Molecular Biology 2023Quote: ... The quantification and quality check of libraries was done using the Bioanalyzer 2100 instrument and the D5000 kit (Agilent Technologies). Libraries were pooled and sequenced on a NovaSeq6000 ...
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bioRxiv - Neuroscience 2023Quote: ... RNA integrity was calculated based on the 28S to 18S rRNA ratio using the RNA 6000 Nano Kit and the Agilent 2100 Bioanalyzer (Agilent Technologies Inc. ...
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bioRxiv - Microbiology 2023Quote: ... The isolation of RNA fragments corresponding to ribosomal footprints of 15 – 45 nt size was verified using the RNA 6000 Nano Kit (Agilent) and the Agilent 2100 Bioanalyzer ...
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bioRxiv - Neuroscience 2023Quote: ... pooled libraries were additionally purified with AMPure beads (ratio 1:1) to remove contaminating primer dimers and quantified using Qubit and the Bioanalyzer High-Sensitivity DNA kit (Agilent). 50-bp paired-end deep sequencing was carried out on HiSeq 4000 (Illumina).
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bioRxiv - Microbiology 2023Quote: ... A R43A PEXEL mutation in UIS21-70-mNG was generated in this plasmid using a QuikChange Lightning Multi Site Directed Mutagenesis kit (Agilent) and the primer P9 ...
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bioRxiv - Immunology 2023Quote: ... and Seahorse XF Cell Mito Stress Test Kit (catalog number 103015-100, contains 1 each of oligomycin, FCCP, and rotenone/antimycin A.) were all purchased from Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... The quality and size distribution of the libraries were assessed on a Fragment Analyzer using the HS NGS Fragment Kit (Agilent). The pooled samples were then sequenced on a NovaSeq6000 (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... All the H2 mutant constructs were subsequently derived from the pEF6-H2R plasmid using a QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent), with mutation accuracy confirmed by sequencing (Genomics Inc. ...
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bioRxiv - Microbiology 2023Quote: ... All subsequent tH2 mutants expressed in bacteria were derived from pET28a-Smt3-tH2 using a QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Quality of the final library preparation was analysed on the Bioanalyzer using the High Sensitivity DNA reagents kit and cassettes (5067-4626, Agilent). For all the steps involving purification of library intermediates ...
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bioRxiv - Developmental Biology 2023Quote: ... all libraries were ligated to NEBNext® Multiplex Oligos for Illumina® (Dual Index Primer Set I) and quality control was performed using a 2100 Bioanalyser High Sensitivity DNA Kit 5067-4626 (Agilent) and libraries quantified using a QubitTM Fluorometer following the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... and oxygen consumption rates (OCR, XF Cell Mito Stress Test) were performed with a Seahorse XF24 Extracellular Flux Analyzer and test kits (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... PCR products are then purified and checked for fragment size distribution on Fragment Analyzer using HS NGS Fragment Kit (1-6000bp) (Agilent).
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bioRxiv - Evolutionary Biology 2023Quote: ... Size distribution and degradation were assessed using the Fragment analyzer (AATI) and High Sensitivity Large Fragment 50 kb Analysis Kit (Agilent). Purification steps were performed using AMPure PB beads (PacBio) ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was extracted using the ZYMO RNA purification kit and RNA integrity was confirmed by the TapeStation system (Agilent). Total RNA was used to generate the sequencing libraries using the KAPA Stranded mRNA Seq kit for the Illumina sequencing platform according to the manufacturer’s protocol (KAPA biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... Extracted DNA was assessed for quantity using the Qubit High Sensitivity dsDNA kit and quality using the Genomic DNA ScreenTape on a TapeStation 4200 (Agilent). Libraries were then prepared for Nanopore sequencing using the PCR-free Rapid DNA sequencing Kit (SQK-RAD004 ...
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bioRxiv - Genetics 2023Quote: ... the Prab-3p::hpk-1 plasmid was used and mutations were performed with a QuikChange II XL Site-Directed Mutagenesis Kit (Agilent). All the assembled plasmids were validated by sequencing prior to microinjection ...
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bioRxiv - Immunology 2023Quote: ... The total RNA quality and quantity were analyzed by Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0 ...
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bioRxiv - Cell Biology 2023Quote: ... we created all other Gαi3 mutants featured in this work using a QuikChange® Site-Directed Mutagenesis Kit as per manufacturer’s protocol (Stratagene, USA). A variant that is insensitive to ADP-ribosylation by Pertussis Bordetella toxin (PTX ...
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bioRxiv - Genetics 2023Quote: ... after which DNA fragments in a range of 400-700 bp were excised and extracted using Gel Extraction Kit (A&A Biotechnology) The quality and quantity of the libraries were verified with TapeStation system (Agilent) and Qubit 2.0 fluorometer ...
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bioRxiv - Immunology 2023Quote: ... The variable heavy chain sequence of CH42 was subcloned into a plasmid coded with an IgG1 heavy chain backbone containing the N297A 29 mutation via QuikChange Site Directed Mutagenesis kit (Agilent). Antibodies were expressed through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher ...