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Citations for Agilent :
5651 - 5700 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Average fragment lengths were determined using the Femto Pulse system with the Genomic DNA 165kb Analysis Kit (FP-1002-0275, Agilent).
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bioRxiv - Zoology 2024Quote: ... The quality of extracted DNA was checked on the Agilent 2100 Bioanalyzer by the High Sensitivity DNA kit (Agilent, US). Because DNA was already fragmented to 50-200 bp fragments ...
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bioRxiv - Microbiology 2024Quote: ... the dual mutations C133S/T135S was introduced to the HP1α CSD (aa 112-176) using the QuikChange Mutagenesis Kit (Stratagene). HP1α was expressed and purified as described above ...
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bioRxiv - Immunology 2024Quote: ... The total RNA quality and quantity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... Key parameters of mitochondrial respiration were measured by applying Seahorse XF Cell Mito Stress Test Kit (#103015-100, Agilent Technologies), which is a set of oligomycin ...
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bioRxiv - Microbiology 2024Quote: SADS-CoV spike mutations were inserted into the SADS glycoprotein encoding plasmid template (pcDNA3.1-SADS-S-HisTag) by site-directed mutagenesis using the QuickChange II XL Site-Directed Mutagenesis Kit (Agilent, 200522) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Each sample was quantified with the Quant-iT Qubit dsDNA HS kit and the library size distribution was confirmed by Agilent TapeStation (HS DNA 1000).
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bioRxiv - Molecular Biology 2024Quote: ... Fragmentation of chromatin to an average size of 200-500bp was checked on Agilent 2100 Bioanalyzer using High Sensitivity DNA Kit (Agilent). Samples were then diluted by adding 1 volume of equilibration buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... the integrity and concentration of RNA were analyzed using an Agilent RNA 6000 Nano Kit (Agilent, cat. no. 5067-1511) and Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Immunology 2024Quote: ... Eluted cDNA was assessed for quality and quantified with the Agilent High Sensitivity DNA Kit and using a Bioananlyzer 2100 (Agilent) instrument ...
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bioRxiv - Immunology 2024Quote: ... The glycolytic function of infected macrophage was analyzed by the Agilent Seahorse XF Glycolytic Stress Test Kit (Agilent, 103020-100). Mφs and amastigotes were distributed in Seahorse XF96 tissue culture microplates for 3 days at 34°C ...
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bioRxiv - Biochemistry 2024Quote: The plasmid for expression of the rare codon reporter pCIneo-RLuc (30xRC) was generated by site-directed mutagenesis using the QuikChange Site-Directed Mutagenesis kit (Stratagene). Protein mutant plasmids used in this study are listed in Supplementary Table S1 ...
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bioRxiv - Genomics 2024Quote: ... RNA integrity (RIN) values for each sample were determined using the Agilent RNA 6000 Nano chip kit (Agilent, 5067-1511) and an Agilent Bioanalyzer 2100 instrument following the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2024Quote: ... and the mean fragment size was assessed using the DNA High Sensitivity KIT on a 2100 Bioanalyzer (Agilent Technologies, Netherlands). 4 nM pooled libraries was sequenced on NovaSeq S1 flow cell by Livestock Improvement Corporation Ltd ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: ... This construct was also used to generate the let-7 ʻseed’ deletion mutant derivative using the QuikChange Multi Site Mutagenesis Kit (catalog 200514-5, Stratagene). 3T3 mouse embryonic fibroblasts (MEFs ...
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bioRxiv - Neuroscience 2024Quote: ... The cDNA libraries were quantified using Fragment Analyzer 5300 (Advanced Analytical) kit FA-NGS-HS (Agilent, Cat#: DNF-474-1000) and Spectramax M2 (Molecular Devices ...
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bioRxiv - Molecular Biology 2024Quote: ... We generated additional LRRK2 variants using the QuikChange Lightning Site-Directed Mutagenesis Kit as per the manufacturer’s instructions (Agilent, #210518).
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bioRxiv - Molecular Biology 2024Quote: ... sequence was introduced between sequences for residues 148 and 149 in KCNQ1 extracellular S1–S2 loop using QuikChange Lightning Site-Directed Mutagenesis kit (Stratagene) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: pFlagN1L11-hSrc and pMycN1L11-Src vectors were described in Aponte et al.18 pFlagN1L11-hSrc -Src-K5R/K7R/K9R (Src 3R) was obtained by mutagenesis using the QuickChange Site-Directed Mutagenesis Kit (Stratagene) using specific oligonucleotides as follows ...
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bioRxiv - Microbiology 2024Quote: ... Quality and quantity of the finished libraries were assessed using an Agilent DNA 1000 kit (Agilent Technologies, Santa Clara, CA) and Qubit® dsDNA HS Assay Kit (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... The deletion constructs of MAD2 as well as BUB1B promoters were generated by omitting one of the estrogen response element (ERE) using Quikchange II site-directed mutagenesis kit (Agilent).
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bioRxiv - Immunology 2024Quote: ... A mitochondrial stress test assay on the XF-HS Mini was optimized within the ranges provided by the manufacturer and performed using the Mitochondrial Stress Test kit (Agilent). In-well concentrations for each drug at the time of injection were Oligomycin (1.5 μM) ...
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bioRxiv - Immunology 2024Quote: ... cDNA and libraries were made using the Lexogen QuantSeq 3’ mRNA-seq FWD library prep kit and quality was assessed by Agilent High Sensitivity DNA kit on a Bioanalyzer (Agilent) ...
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bioRxiv - Genetics 2024Quote: ... RNA concentration and purity was measured on a Nanodrop and with Agilent RNA 6000 Nano Kit (Agilent Technologies, 5067-1511) on Agilent 2100 Bioanalyzer ...
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bioRxiv - Immunology 2024Quote: ... cDNA was stored for a maximum of 4 weeks at –20°C before making the libraries and concentration was assessed using a Bioanalyzer High Sensitivity DNA Kit on a 2100 Bioanalyzer (Agilent). To generate libraries ...
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bioRxiv - Cancer Biology 2024Quote: ... Pre-hybridization library was produced using SureSelect XT HS and XT low input library preparation kit (Agilent Technologies, CA, USA) and barcoding was performed using Illumina 8-nt dual-indices ...
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bioRxiv - Immunology 2024Quote: ... OCR and ECAR were analyzed using a Seahorse XFp Cell Mito Stress Test kit (Agilent Technologies, Santa Clara, CA, USA). All assays were performed according to manufacturer instructions ...
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bioRxiv - Genomics 2024Quote: ... The final product was assessed for size distribution and concentration using the BioAnalyzer High Sensitivity DNA Kit (Agilent, 5067-4626). Pooled libraries were diluted to 2 nM in EB buffer (Qiagen ...
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bioRxiv - Biochemistry 2024Quote: ... The BTSL2 E98A and E664A variants were made by site-direct mutagenesis using the listed primers and QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent) according to the supplier’s manual.
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bioRxiv - Genomics 2024Quote: ... The high-SBP rs9833313-T allele was introduced by site-directed mutagenesis using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and confirmed by sequencing ...
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bioRxiv - Genetics 2024Quote: ... and RNA integrity Number (RIN) was measured by using a TapeStation system with an RNA ScreenTape Analysis kit (Agilent Technologies).
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bioRxiv - Biochemistry 2024Quote: ... OXA-163 and OXA-405 vectors were then generated from pUBYT OXA-48 using QuikChange Lightning site-directed mutagenesis kit (Agilent) (primers listed in Table S5) ...
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bioRxiv - Cancer Biology 2024Quote: ... according to manufacturer’s instructions and the quality was assessed with the Agilent 2100 Bioanalyzer with an RNA 6000 Pico Kit (Agilent Technologies). PolyA- enriched RNA libraries were prepared with the KAPA Stranded mRNA Sample Preparation Kit (Kapa Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... The construct expressing the mutant FBXW11F517S protein was generated by PCR-based site directed mutagenesis using the QuiKChange II Site-Directed Mutagenesis Kit (Agilent), as previously described101 ...
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bioRxiv - Cancer Biology 2024Quote: ... The DIRT domain was deleted from pLenti6-3xHA-MARCHF8 using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, 200522) and the primers listed in table S2 ...
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bioRxiv - Cell Biology 2024Quote: ... 1247-1249GGG>GG) were introduced into the GFP-GFAP plasmid by using the Quickchange XL Site-directed Mutagenesis kit (Agilent) or the NZYMutagenesis kit (NZYtech) ...
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bioRxiv - Bioengineering 2024Quote: ... sections were washed in PBS three times for 3 minutes each before being incubated with DAKO Rabbit/Mouse HRP Kit-provided HRP (Mouse-K4001, Rabbit-K4003; Dako) for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... The LZK Q240S drug-resistant construct and K195M kinase-dead mutant (1) was introduced using a Site-Directed Mutagenesis Kit (Stratagene) (Primers listed in Supplementary table S2) ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were generated following manufacturer’s instructions and amplified cDNA and final libraries quality was probed using high sensitivity DNA D5000 and D1000 tape station kits (Agilent). Libraries were sequenced on NovaSeq platform (SP flow cell ...
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bioRxiv - Cancer Biology 2024Quote: ... The assay was performed according to the manufacturer’s instructions in the Seahorse XF Cell Mito Stress Test Kit User Guide (103016-400, Agilent Technologies). 8.0×105 B-cells or 6.0×105 myeloid cells per well were plated on a Seahorse Plate for this assay ...
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bioRxiv - Cell Biology 2024Quote: ... and the quality of DNA samples was assessed by an Agilent 2100 Bioanalyzer using an Agilent High Sensitivity DNA kit (5067-4626; Agilent). cDNA libraries were synthesized by the ThruPLEX DNA-seq Kit (R400675 ...
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bioRxiv - Biochemistry 2024Quote: The A30P αSyn plasmid was prepared by site-directed mutagenesis of the pT7-7 WT αSyn plasmid using a QuikChange II Site-Directed Mutagenesis Kit (Agilent, Agilent Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... between the TEV (Tobacco Etch Virus) protease cleavage site and the open reading Frame (ORF) using a QuickChange mutagenesis kit (Agilent) and then transformed into BL21(DE3 ...
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bioRxiv - Biochemistry 2024Quote: ... GFP-GR variants for RNA-seq and N&B were generated with the QuikChange II XL Site-Directed Mutagenesis Kit (Stratagene) or by Gene Universal.
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bioRxiv - Cancer Biology 2024Quote: ... An aliquot of this chromatin was used to assess the size of the DNA fragments obtained by High Sensitivity NGS Fragment Analysis Kit on a Fragment Analyzer (Agilent). For each sample ...
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bioRxiv - Biochemistry 2024Quote: ... and P221 was mutated to a leucine residue in the GST-PHRF1RP construct to create GST-PHRF1RP(P221L) using a QuikChange II Site-Directed Mutagenesis Kit from Agilent Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Amplified cDNA’s quality and quantity was assessed on an Agilent BioAnalyzer 210 using a High Sensitivity DNA Kit (Agilent Technologies) and the final libraries on an Agilent TapeStation 420 using High Sensitivity D1000 ScreenTape (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... T140A/D SETD8 cDNAs were generated using the QuikChange Lightning Site-Directed Mutagenesis Kit according to the manufacturer’s instructions (Agilent #210518).
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bioRxiv - Cancer Biology 2024Quote: ... Optimal library amplification was assessed by qPCR and by using High Sensitivity NGS Fragment Analysis Kit on a Fragment AnalyzerTM (Agilent). Libraries were then purified using AMPure XP Reagent (Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: The mitochondrial activity of Caco-2 and CMT-93 cells as well as intestinal organoids were measured in real-time using the Seahorse XF Cell Mito Stress Test Kit according to the manufacturer’s instructions on a Seahorse XFe24 Analyzer (Agilent Technologies). Three independent experiments were performed with n=9 replicates ...