Labshake search
Citations for Agilent :
5301 - 5350 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... The fragment size estimation of the resulting libraries was assessed with High SensitivityTM HS DNA kit runned on 2100 Bioanalyzer (Agilent) and quantified using the QubitTM dsDNA High Sensitivity HS assay (ThermoFisher Scientific) ...
-
bioRxiv - Cell Biology 2020Quote: ... The fragment size estimation of the resulting libraries was assessed with High SensitivityTM HS DNA kit runned on 2100 Bioanalyzer (Agilent) and quantified using the QubitTM dsDNA High Sensitivity HS assay (ThermoFisher Scientific) ...
-
bioRxiv - Immunology 2020Quote: ... the quality of RNA was first checked by assessing the RNA integrity number (RIN) using the Agilent RNA 6000 Nano kit (5067-1511, Agilent) and the Agilent 2100 Bioanalyzer Automated Analysis System (Agilent) ...
-
bioRxiv - Pathology 2021Quote: ... Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent) according to the manufacturer’s instructions ...
-
BCL-XL blockage in TNBC models confers vulnerability to inhibition of specific cell cycle regulatorsbioRxiv - Cancer Biology 2021Quote: ... RNA integrity was assessed using the Agilent RNA 6000 Pico kit and Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, California) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... Library quality and quantity was assessed on a Bioanalyzer using the DNA High Sensitivity Kit reagents and protocol (Agilent Technologies). The quantified libraries were pooled together at a final concentration of 100pM ...
-
bioRxiv - Genetics 2021Quote: ... The HiC library was then hybridized with the custom capture baits and captured by the SureSelect XT HS and XT low input library preparation kit for ILM (Agilent). 15 Capture C libraries were made from 5 human primary melanocyte cultures (C56 ...
-
bioRxiv - Pathology 2020Quote: ... RNA sample integrity was evaluated and concentration was measured using an Agilent 2100 Bioanalyzer with an RNA 6000 Pico Kit (Agilent). Libraries for 16 samples were prepared using the Ion AmpliSeq™ Transcriptome Mouse Gene Expression Kit ...
-
bioRxiv - Physiology 2021Quote: RNA was checked for quality using the Agilent RNA 600 nano kit and Bioanalyser instrument (Agilent Technologies, Santa Clara, CA). Aliquots of RNA (1 µg ...
-
bioRxiv - Cancer Biology 2020Quote: ... GST-CD1 DENN V226K/I228D/L235D/Y238K and GST-CD1 DENN M243R/P244R/Q362A were generated using the QuickChange lightning site-directed mutagenesis kit from Agilent Technologies ...
-
bioRxiv - Systems Biology 2020Quote: ... and 1 μL was used to check the expected size (~300 bp) and the purify was verified with the Agilent High Sensitivity DNA Kit as manufacturer’s recommendation (Agilent Technologies). One μL of the diluted libraries were used to quantify as recommended in the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... The final product was assessed for its size distribution and concentration using a BioAnalyzer High Sensitivity DNA Kit (Agilent Technologies). The libraries were pooled and diluted to 3 nM using 10 mM Tris-HCl (pH 8.5 ...
-
bioRxiv - Genomics 2020Quote: ... Quality of total RNA was assessed on an Agilent BioAnalyzer 2100 using the RNA 6000 Nano kit (Agilent, # 5067-1511). All samples had a RIN > 7.6 ...
-
bioRxiv - Cancer Biology 2021Quote: ... S200C and S200T) were introduced by site directed mutagenesis using QuikChange Lightning Site-Directed Mutagenesis Kit according to manufacturer’s protocol (Agilent Technologies). To express PRSS2 WT and Mutants protein ...
-
bioRxiv - Molecular Biology 2020Quote: ... and then analyzed by using the Agilent Tapestation 4200 System and High Sensitivity DNA D5000 kit (Agilent Technologies, CA, USA) to determine quality and quantity of the amplicons.
-
bioRxiv - Genetics 2021Quote: ... while the domain deletions were constructs from initial cDNA constructs using the site-directed mutagenesis kit QuikChange™ (Agilent Technologies) and adequate primers (Supplemental Table S4) ...
-
bioRxiv - Plant Biology 2021Quote: ... and quality (RNA integrity > 8.5) of RNA was determined using a Bioanalyzer 2100 RNA 6000 Pico Total RNA Kit (Agilent Technologies). Samples containing >5 μg of RNA in total were used for RNAseq ...
-
bioRxiv - Genomics 2021Quote: ... Final quality control of the purified RNAs was performed using the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip Kit (Agilent) and the Qubit Fluorometer with the Qubit RNA BR (Broad-Range ...
-
bioRxiv - Microbiology 2021Quote: ... The specific variants were generated by site-directed mutagenesis in pCMV6-Entry-YTHDF2 using the QuikChange II site-Directed Mutagenesis Kit (Cat# 200521, Stratagene) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... and Halo-V5-WTAP mutants (D301A and D301A/D302A) were generated using the QuikChange II site-directed Mutagenesis Kit (Stratagene) following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... PartB RNA was quantified and purity/quality was assessed on the 2100 Agilent BioAnlayzer using the Agilent small RNA kit (5067-1548, Agilent).
-
bioRxiv - Genetics 2020Quote: ... The A>T variant was introduced to PIK3C2B-pNL construct by site-directed mutagenesis using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) to generate PIK3C2B-A>T-pNL construct ...
-
bioRxiv - Genetics 2020Quote: ... WGS library-size selection was then verified using the 2100 Bioanalyzer DNA 1000 Kit (Agilent Technologies, Santa Clara, CA, USA). The WGS library was then sequenced (2×150 bp ...
-
bioRxiv - Genetics 2020Quote: ... in the nmt1-GFP-swi6+ expression construct (kindly gifted by Dr. Alison Pidoux) using the QuickChange site-directed mutagenesis kit (Stratagene), as per the manufacturer’s instructions ...
-
bioRxiv - Genetics 2020Quote: ... plasmid using oligonucleotides containing the SHRF syndrome-linked G58V and G226D missense mutations and the QuikChange Site-Directed Mutagenesis Kit (Stratagene). The untagged RRP4/rrp4-G226D (pAC3656 ...
-
bioRxiv - Genetics 2020Quote: The BRCA2 and PALB2 constructs were made siRNA resistant by introducing three silent mutations with the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent), according to the manufacturer’s protocol ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Site-directed mutagenesis of Aγ was performed using the QuikChange II XL Site-directed Mutagenesis Kit (Agilent, Santa Clara, CA). cRNAs were transcribed using mMessage mMachine Transcription Kits (Invitrogen) ...
-
bioRxiv - Cell Biology 2021Quote: ... W224A mutants were obtained in the pcDNA5 His PC TEV Arpin plasmid using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent). ORFs encoding Arpin WT and mutant forms were subcloned between FseI and AscI sites into custom-made pcDNAm FRT PC GFP ...
-
bioRxiv - Genomics 2021Quote: ... Concentration and quality of the libraries were measured using Qubit® 2.0 Fluorometer and Bioanalyzer High Sensitivity DNA Analysis kit (Agilent), respectively ...
-
bioRxiv - Plant Biology 2021Quote: ... The total RNA quality was assayed using an Agilent RNA 6000 Nano Kit Bioanalyzer chip (Agilent Technologies, St. Clara, USA). Labelling was done following the Low Input Quick Amp Labelling protocol of the Two-Color Microarray-Based Gene Expression Analysis system (Agilent Technologies ...
-
Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imagingbioRxiv - Molecular Biology 2021Quote: The first yeast library (called library A in this study) of FAST constructed by error-prone PCR using Genemorph II kit (Agilent) was previously described23 ...
-
bioRxiv - Neuroscience 2021Quote: ... Both Southern blot probes were generated by standard PCR and subjected to random labelling using a Prime-It II Random Primer Labelling Kit (#300385, Agilent) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... The RNA integrity was determined by microfluidic capillary electrophoresis using the Agilent RNA 6000 Nano Kit and the 2100 Bioanalyzer Instrument (Agilent).
-
bioRxiv - Molecular Biology 2020Quote: ... Mutation of the near-cognate codon AAG to CAG was performed using a QuikChange II XL Site-Directed Mutagenesis Kit (Agilent). All generated constructs were verified by Sanger sequencing.
-
bioRxiv - Evolutionary Biology 2021Quote: ... We extracted RNA using the Qiagen RNeasy Blood and Tissue Kit and checked RNA integrity with a Bioanalyzer 2000 (Agilent) or TapeStation 2200 (Agilent) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Libraries were constructed using Illumina Nexterna XT kit and were analyzed for concentration by Qubit and for size distribution by Agilent Bioanalyzer ...
-
bioRxiv - Microbiology 2021Quote: ... Additional RBD mutations were introduced into the Delta spike also using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent). The primers for mutagenesis were designed on Agilent’s website (https://www.agilent.com/store/primerDesignProgram.jsp) ...
-
bioRxiv - Microbiology 2021Quote: ... D950N) were prepared from wild-type SARS-CoV-2 spike using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent). Additional RBD mutations were introduced into the Delta spike also using the QuickChange Lighting Multi Site-directed Mutagenesis kit (Agilent) ...
-
bioRxiv - Cell Biology 2021Quote: ... The three missense mutations identified were introduced into a previously described pcDNA3.1-CD33-V5/HIS vector using a QuikChange XL kit (Agilent 200517) with forward primer 5’-GCACTTGCAGCCGGATTTTTGGATCCATAGCCAGGGCC and reverse primer 5’-GGCCCTGGCTATGGATCCAAAAATCCGGCTGCAAGTGC (Invitrogen ...
-
bioRxiv - Developmental Biology 2021Quote: ... The D238A catalytically deficient fbl mutant was generated by site-directed mutagenesis with the help of the QuikChange II site-directed mutagenesis kit (Stratagene) and primers LD3881 and LD3882 ...
-
bioRxiv - Developmental Biology 2021Quote: ... or the Illumina TruSeq Stranded Total RNA Library Prep kit (medaka hybrid and mCherry or KDM4E-injected zebrafish samples) and checked using a Fragment Analyzer System (Agilent) before multiplexing ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: The pGEX6P1-Hecw C1394W construct was generated by site-directed mutagenesis according to the QuikChange Site-Directed Mutagenesis Kit protocol (Agilent) using the following primers ...
-
bioRxiv - Biochemistry 2021Quote: ... 67)) plasmid as PCR template Bri2 BRICHOS D148N was obtained with QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, US), and the DNA sequence was confirmed (GATC Bioteq ...
-
bioRxiv - Cancer Biology 2021Quote: ... The amount and average fragment length of amplified RNA was determined using the RNA 6000 Pico Kit (Agilent, 5067-1513) with a 2100 Bioanalyzer according to the manufacturer’s protocol.
-
bioRxiv - Biochemistry 2021Quote: Mutations were introduced to LRH-1 in the pMSC7 and pCI vectors using the Quikchange Lightning Site-Directed Mutagenesis Kit as directed by the manufacturer (Agilent). Constructs were sequenced prior to use.
-
bioRxiv - Biochemistry 2021Quote: ... Parkin mutant plasmids were cloned beginning with a Homo sapiens wild type eGFP-parkin vector [36] and performing PCR point mutagenesis (Quikchange Mutagenesis kit: Stratagene) utilizing primers ordered from Invitrogen ...
-
bioRxiv - Biochemistry 2021Quote: ... MYC-binding sites (E-boxes with the sequence CACGTG at positions -1149 bp and -1353 bp upstream of the transcription start site of the PTEN gene) were mutated to CAAGAA using Quikchange Lightening kit (Agilent). The TATA synthetic promoter sequence was derived from pGL firefly reporter with a minimal promoter.
-
bioRxiv - Biochemistry 2020Quote: ... This construct was expressed in the HEK cell line and regenerated with 11-cis-retinal.22 MRh was prepared similarly.22 Site-directed mutagenesis was performed using the QuikChange Multisite-Directed Mutagenesis Kit (Agilent Technologies ...
-
bioRxiv - Cancer Biology 2021Quote: ... pCMVHA hEZH2 and V5-EZH2 vector was used to generate EZH2-H689A mutant vector using the mutagenesis primers listed in Table S1 with QuikChange II site-directed mutagenesis kit (Agilent) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... DNA quality of the samples was assessed with the Fragment AnalyzerTM and the DNF-488 High Sensitivity genomic DNA Analysis Kit (Agilent). DNA was slightly more fragmented than defined by the quality control standards but this fragmentation was minor ...