Labshake search
Citations for Agilent :
5251 - 5300 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... All mutant constructs used in this study were generated from this construct either by a QuickChange II XL kit (Stratagene) or a Q5® Site-directed mutagenesis kit (New England Biolabs ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated and purified using the Absolutely RNA Miniprep Kit (Cat# 400800, Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s protocol for extremely small samples ...
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bioRxiv - Neuroscience 2021Quote: ... KCNQ2 mutants were generated by site-directed mutagenesis using a QuikChange kit according to manufacturer’s protocol (Stratagene, San Diego, CA). cRNA transcripts encoding human KCNQ1-5 were generated by in vitro transcription using the T7 polymerase mMessage mMachine kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... after quality control using the Agilent 2100 Bio analyzer (Agilent RNA 6000 Nano Kit, Agilent Technologies, Santa Clara, CA, USA), poly-T oligo-attached magnetic beads were used to purify poly-A containing mRNA ...
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bioRxiv - Neuroscience 2021Quote: ... TDP-43N-Del was generated by deletion of the first 81 amino acids from the N-terminus of TDP-43WT using the QuickChange Site-Directed Mutagenesis Kit (Agilent).
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bioRxiv - Neuroscience 2021Quote: ... The 481 bp ywhaz amplicon was generated by PCR reaction and cloned into a plasmid using the StrataClone PCR cloning Kit (Agilent). The plasmids were collected and purified using GeneJET Plasmid Maxiprep Kit (Thermo Scientific ...
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bioRxiv - Genomics 2020Quote: ... quality of libraries was assessed using a high sensitivity Small DNA Fragment Analysis Kit (Agilent Technologies, Santa Clara, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Kinase dead catalytically inactive versions of Drosophila and human RIOK2 (dRIOK2123A,246A, RIOK2123A,246A) were created using site directed mutagenesis (Lightning Quick Change Kit, Agilent) to make alanine substitutions at catalytic residues Lys-123 and Asp-246 in both human RIOK2 and dRIOK2 as described by other groups (22 ...
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bioRxiv - Molecular Biology 2020Quote: ... were introduced using RE specific primers (Table 1 and Table 2 in supplementary material) by QuickChange Multi site-directed mutagenesis kit (Agilent) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... cDNA library quality was quantified by 2100 Bioanalyzer using Agilent High Sensitivity DNA Kit (#5067-4626, Agilent, Santa Clara, CA). Library was sequenced as 101 bp paired-end on Illumina NovaSeq6000.
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bioRxiv - Biochemistry 2021Quote: ... Mutations of the two Walker-A motifs in human ABCF1 (K324M, K664M) were created by using Quikchange II Site Directed Mutagenesis Kit (Agilent). Human ABCF1 and yeast GCN20 domain fusion cDNAs were created by PCR-mediated ligation and cloned into modified pMtac-His6 vector ...
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bioRxiv - Neuroscience 2020Quote: ... An AgeI site was inserted into exon 1 of Cacna1b by mutagenesis using a QuickChange II XL site-directed mutagenesis kit (Stratagene) with primers 1-For and 1-Rev ...
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bioRxiv - Immunology 2020Quote: ... The libraries were analyzed for size and quantity of DNAs on a 2100 Bioanalyzer using a High Sensitivity DNA kit (Agilent), diluted and pooled in multiplex sequencing pools ...
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bioRxiv - Molecular Biology 2022Quote: ... Final size selected libraries were checked for quality on the Agilent BioAnalyzer with High Sensitivity DNA analysis kit (5067-4626, Agilent) to confirm proper size selection ...
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bioRxiv - Molecular Biology 2022Quote: ... The final libraries were assessed on a fragment analyzer using the High Sensitivity NGS Analysis Kit (Agilent, DNS-474-FR) and sequenced using the NextSeq550 Illumina platform (75-bp paired-end reads) ...
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bioRxiv - Neuroscience 2022Quote: ... following the manufacturer’s protocol and RNA quality was assessed using the RNA 6000 Pico Kit on a 2100 Bioanalyzer (Agilent Technologies). RNA sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... The cDNA libraries were quantified using Fragment Analyzer 5300 (Advanced Analytical) kit FA-NGS-HS (Agilent, Cat#: DNF-474-1000) and Spectramax M2 (Molecular Devices ...
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bioRxiv - Cancer Biology 2022Quote: ... WES libraries were generated by exome capture of approximately 20,000 coding genes using SureSelect human All exon V6 kit (Agilent Technologies) and paired-end sequencing was performed on a HiSeq sequencer (Illumina ...
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bioRxiv - Genetics 2022Quote: ... This variant was deemed a SNP without functional consequences but subsequently corrected and other missense variants were generated by QuickChange Multi Site-Directed Mutagenesis Kit (Agilent). WT and missense variant cDNAs were transferred from pDONR223 to MSCV_IP_N terminal-HA-FLAG vector using Gateway recombination (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... Library size and quality was checked using an Agilent 2100 Bioanalyzer with the High Sensitivity DNA kit (Agilent, #5067–4626) or the Fragment Analyzer CRISPR discovery kit ...
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bioRxiv - Developmental Biology 2022Quote: ... The coding exons and flanking intronic regions were enriched with the SureSelectXT Low Input All Exon v7 kit (Agilent Technologies) followed by dual index ...
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bioRxiv - Microbiology 2022Quote: ... pcDNA-VP4-KpnI/BamHI was built by insertion of point mutations in pcDNA-VP4-SA11 using the QuikChange site-directed mutagenesis kit and protocol (Agilent) to insert KpnI and BamHI restriction sites in VP4 ...
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bioRxiv - Genomics 2022Quote: ... The quantity of extracted HMW DNA was evaluated with the HS Qubit DNA kit and the fragment profile and overall quality was assessed with the Femto Pulse instrument (Agilent). Pacific Biosciences (PacBio ...
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bioRxiv - Pathology 2022Quote: Exonic sequences were enriched in the DNA sample of patient II-6 using SureSelect Human All Exon 50 Mb Kit (Agilent Technologies ...
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bioRxiv - Immunology 2022Quote: ... and quality assessed using the RNA 6000 Pico Kit on the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA).
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bioRxiv - Evolutionary Biology 2022Quote: We isolated the plasmids containing the three segregating lacZ promoter variants from the MG1655 strain using StrataPrep Plasmid Miniprep Kit (Agilent). We also isolated the promoter- less vector pMV001 the same way ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We then ran the digested products on 1% agarose gel and extracted the desired fragments using StrataPrep DNA Gel Extraction Kit (Agilent). We then assembled these two fragments using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Genomics 2022Quote: ... Mutagenesis was carried out to introduce the H840A mutation into eSpCas9(1.1) variant using pJSC114 plasmid (addgene, 101215) and QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, 210518). eCas9 nickase was expressed using the mutagenesis-modified pJSC114 plasmid ...
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bioRxiv - Genetics 2022Quote: ... An AgeI site was introduced upstream of the SV40 polyA signal and the BamHI site downstream of the SV40 polyA signal was deleted using the QuikChange Lightning site-directed mutagenesis kit (Agilent). Synthesized oligos were amplified with 0.05pmol of template per 50μL PCR reaction for seven cycles using MPRA library amplification primers ...
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bioRxiv - Immunology 2022Quote: Seahorse assays were performed according to the manufacturer’s instructions to analyze glycolysis metabolism using the Seahorse XF Glycolysis Stress Test Kit (Agilent Technologies). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... Quality control of cDNA and scRNA-seq libraries were performed using the Bioanalyzer High Sensitivity kit (Agilent Technologies Inc., CA). Libraries were sequenced using Novaseq 6000 (Illumina Inc. ...
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bioRxiv - Plant Biology 2022Quote: ... Mutations were inserted into MP and GFP:MP plasmid with the QuickChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) using primers in Table S5.
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bioRxiv - Immunology 2022Quote: ... Slides were washed in PBS and incubated with horseradish peroxidase (HRP) conjugated secondary antibody included in the peroxidase substrate kit (Dako EnVision + System HRP labelled polymer anti-mouse or anti-rabbit IgG ...
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bioRxiv - Cancer Biology 2022Quote: ... V37W) from pLOC-NT5C2 plasmid by site directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent Technologies) according to manufacturer’s guidelines.
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bioRxiv - Cell Biology 2022Quote: ... Site directed mutagenesis was done using a QuikChange® Lightning Site Directed Mutagenesis kit (Agilent Technologies, Inc., Santa Clara, CA).
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bioRxiv - Cell Biology 2022Quote: ... Quantitation and quality assessment of each of the 12 libraries were performed on 2200 Tapestation analyzer using the High Sensitivity D1000 ScreenTape kit (Agilent).
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bioRxiv - Genetics 2020Quote: ... genomic DNA from various tissues (Supplemental Table 6) was purified and used for library construction with target enrichment using the SureSelectQXT Target Enrichment kit (Agilent). Custom RNA capture probes were designed to hybridize with the 120 bp 5’ ends of the 5’LTRs and the 120 bp 3’ ends of the 3’LTR of about 600 intact (internal region flanked by two LTRs ...
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bioRxiv - Developmental Biology 2020Quote: ... Single positions within gene sequences were modified by site-directed mutagenesis applying the QuikChange II kit according to manufacturer’s protocol (Agilent Technologies).
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bioRxiv - Developmental Biology 2020Quote: ... To generate the Sp ΔBTD-box and the btd ΔBTD-box we used the QuickChange Site-Directed Mutagenesis Kit (Stratagene) to delete the 30 bp encoding for the BTD-box using the following primers:
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bioRxiv - Cell Biology 2020Quote: ... 2xmyc-RAB10-T73A and 2xmyc-RAB35-T72A were made by directed mutagenesis from the WT vectors using the QuickChange Lightning kit (Agilent). EGFP-RAB10 (Addgene#49472)(52) ...
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bioRxiv - Cell Biology 2020Quote: ... A wobble mutation was made at an internal KpnI site in mEos3.2 to prevent its digestion using the QuikChange II XL Site-Directed Mutagenesis kit (200521; Agilent Technologies) and following primer pair ...
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bioRxiv - Cell Biology 2020Quote: ... First-strand cDNA was synthesized by reverse transcribing 500 ng of total RNA per sample using either the AffinityScript qPCR DNA Synthesis kit (Agilent), or the LunaScript RT Super Mix Kit (NEB # E3010(S/L) ...
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bioRxiv - Cell Biology 2020Quote: ... Different point mutations were introduced into SHANK3 constructs by site-directed mutagenesis (Gene Universal) or by using mutagenic oligonucleotides and the Quick-Change II site-directed mutagenesis kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was reversed transcribed with MARS-seq barcoded RT primers in a 10 μl volume with the Affinity Script kit (Agilent). Reverse transcription was analyzed by qRT-PCR and samples with a similar CT were pooled (up to eight samples per pool) ...
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bioRxiv - Plant Biology 2020Quote: ... To obtain the inward open state the mutations E162Q and D344N were introduced using the quickchange site-directed mutagenesis kit (Agilent). Transformed Saccharomyces cerevisiae (strain DSY-5 ...
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bioRxiv - Bioengineering 2020Quote: ... Site-directed mutagenesis to change SB sequences was carried out using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent technologies) and specifically designed primers.
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bioRxiv - Molecular Biology 2021Quote: ... gambiae, a 15k-probe microarray (Agilent; A-MEXP-2196 [14], using the Agilent gene expression hybridization kit (Agilent Technologies, USA). Slides were washed according to the manufacturer’s instructions and scanned on an Agilent G2565CA microarray scanner ...
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bioRxiv - Genetics 2021Quote: ... Partition barcoded libraries were then prepared based on the Chromium Exome Solution (10X Genomics) and the exome target regions was enriched by SureSelect Human All Exon V5 Kit (Agilent) according to the protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... In order to generate the mutant expression plasmids for BMAL2 we used the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (210515, Agilent). The bHLH mutant was generated by converting an arginine to alanine (OaBMAL2_R88A ...
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bioRxiv - Microbiology 2021Quote: Site-directed mutagenesis of GFP-ORF57 for W292A mutant was carried out using QuikChange site-directed mutagenesis kit (Agilent technologies) as per manufacturer’s instructions.