Labshake search
Citations for Agilent :
5101 - 5150 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... immunohistochemistry was performed by using the streptavidin-biotin-peroxidase method with diaminobenzidine as the chromogen (Kit LSAB, Dakocytomation, Glostrup, Denmark). Negative controls were obtained after omission of the primary antibody or incubation with an irrelevant antibody.
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bioRxiv - Biochemistry 2022Quote: ... The various mutant forms of this plasmid were made with the QuikChange II XL Site-Directed Mutagenesis Kit from Agilent using the appropriate primers each time (Table S4) ...
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bioRxiv - Developmental Biology 2022Quote: ... Specific point mutations in Hmga1 3’UTRs (shown in Fig 3A) cloned into pRL-CMV vector were generated using site-directed mutagenesis kit (Stratagene). In addition ...
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bioRxiv - Cell Biology 2022Quote: ... K22M mutation of the kinase dead PMVK mutant (PMVKmut) was generated using QuickChange Site-Directed Mutagenesis Kit (Agilent, Catalog # 200518). Lentiviral particles were used to constitutively express shPMVK (pLV[shRNA]-Hygro-U6> hPMVK[shRNA#4] ...
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bioRxiv - Biochemistry 2022Quote: ... P286R and V411L variants were obtained by carrying out point mutation to these clones using the QuikChange II site directed mutagenesis kit (Agilent). The oligonucleotides used to produce these mutations are shown in Table 2 ...
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bioRxiv - Biochemistry 2022Quote: The mutations S26C and K28C were introduced to SD-Aβ40 fusion protein19 via site-directed mutagenesis using a QuikChange Lightning Kit (Agilent, USA) following a modified protocol.22 The successful introduction of mutations was confirmed by sequencing (Genewiz) ...
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bioRxiv - Neuroscience 2021Quote: ... quality and quantity of both transcriptome and translatome samples were evaluated in an Agilent 2100 Bioanalyzer using Nano and Small RNA kits (Agilent Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... The D614G amino acid change was introduced into VRC7480 by site-directed mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit from Agilent Technologies (Catalog # 210518) ...
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bioRxiv - Molecular Biology 2020Quote: ... Site-directed mutagenesis to generate the miCLIP-mutants was performed using the QuikChange II Site-Directed Mutagenesis Kit from Agilent as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Size-selected libraries were verified using the Agilent 2100 Bioanalyzer with the High Sensitivity DNA kit (Agilent, cat# 5067-4626) and quantified using KAPA Library Quantification Kits (KAPA Bio ...
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bioRxiv - Molecular Biology 2021Quote: RNA purified from polysomal fractioning (the reconstituted total mRNA and pooled polysomal RNA) was tested for quality using a RNA 6000 nano kit (Agilent) on a Bioanalyzer 2100 (Agilent).
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bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated secondary antibodies were then incubated on slides for 1-hour and detected with the DAB+ chromogen kit (DAKO). After washing ...
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bioRxiv - Molecular Biology 2020Quote: ... 154 mutated to leucine) was generated using the QuickChange Site Directed Mutagenesis Kit according to the manufacturer’s protocol (Agilent #200519). The BirA Ligase containing plasmid was purchased (Addgene #64395) ...
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bioRxiv - Molecular Biology 2020Quote: The plasmid encoding NusG-F165A was generated by site-directed mutagenesis according to the QuikChange Site-Directed Mutagenesis Kit (Stratagene), using vector pET11A_nusG (Burmann et al. ...
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bioRxiv - Cell Biology 2022Quote: ... of hPASMC were measured using Seahorse XF96 metabolic analyzer and Seahorse XF Cell Mito Stress Test kit (103015-100, Seahorse XF Technology, Agilent), following manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... Other variants containing single or multiple amino-acid changes in the spike gene from wildtype or D614G were made by mutagenesis using QuickChange lightning Multi Site-Directed Mutagenesis Kit (cat # 210515, Agilent) or via synthesis and cloning (Genscript) ...
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bioRxiv - Genomics 2020Quote: ... the amplified libraries were purified using a Qiagen MinElute PCR Purification Kit and eluted in 20 μl Elution Buffer before quantitation on an Agilent 4200 TapeStation (Agilent Technologies LDA UK Limited ...
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bioRxiv - Immunology 2021Quote: ... Extracted RNA samples were then quantified and qualified using a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, USA) with RIN number >7.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Quality of cDNA libraries were studied with BioAnalyzer DNA analysis (5067-1504, Agilent, Agilent High Sensitivity DNA kit, 5067-4626). Final DNA concentration was determined with Quantus Fluorometer (E6150 ...
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bioRxiv - Molecular Biology 2021Quote: ... Mutant Mul DNA gyrases (Ala91Val or Asp95Gly) were generated from WT gyrA and gyrB using a QuickChange site-directed mutagenesis kit (Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Final and cDNA libraries were then QCed on a bioanalyzer (Bioanalyzer High Sensitivity DNA kit: Agilent Technologies, Inc., 5067-4626) and qubit (dsDNA high sensitivity kit ...
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bioRxiv - Cell Biology 2020Quote: ... substitution of Cysteine to Alanine at 548 in Py-S2 was introduced using a site-directed mutagenesis QuikChangeII kit (Agilent) by following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Library validation included size and purity assessment with the Agilent 2100 Bioanalyzer and the Agilent DNA 1000 kit (Agilent Technologies). Samples were diluted to 10nM and pooled to create the 12X multiplexed DNA libraries ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Deletion and site-directed mutants were generated in human ENaC cDNAs cloned into a pGEM HE vector using the QuikChange II Site-Directed Mutagenesis kit (Stratagene) (Ji & Benos ...
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bioRxiv - Cancer Biology 2020Quote: ... The DNAJB1-PRKACA K128H kinase-dead mutant was cloned using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) using the following primers (5’-CCTTCTGTTTGTCGAGGATATGCATGGCATAGTGGTTCCCG-3’ and 5’-CGGGAACCACTATGCCATGCATATCCTCGACAAACAGAAGG-3’) ...
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bioRxiv - Molecular Biology 2020Quote: ... the gene of FASTD71V,P73T was randomly mutagenized by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent). The PCR product was digested with NheI and BamHI ...
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bioRxiv - Biochemistry 2020Quote: Desired mutations were introduced into mhsT wild-type gene via site-directed mutagenesis (Mutagenesis QuikChange Lightning Kit, Agilent Technolgies, Inc) using designed primers ...
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bioRxiv - Biochemistry 2021Quote: ... RpoB E310A and RpoB E310K were made by site-directed mutagenesis of the wild type constructs using the Quikchcange II XL kit (Agilent). B ...
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bioRxiv - Biochemistry 2020Quote: ... The pcDNA3.1 Hygro-DEST_VGLL2_Ω_loop mutant was generated by use of the QuikChange Lightning Site-Directed Mutagenesis kit (Stratagene, San Diego, CA), with oligo 5’CCC TTG GAG AGA CTG CAG CTT CTA TCA GGC TCC TGT GCC 3’ (removing amino acid positions 134-149 ...
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bioRxiv - Microbiology 2021Quote: ... Quantity and quality of RNA was assessed on an Agilent 2100 Bioanalyzer using Agilent RNA 6000 Nano kit (Agilent, USA). Extracted RNA was reverse-transcribed to cDNA using SuperScript™ IV First-Strand Synthesis System (Invitrogen™ ...
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bioRxiv - Genomics 2020Quote: ... Total RNA integrity and quantity of the samples were assessed using the Agilent 2100 Bioanalyzer with the RNA 6000 Nano LabChip Kit (Agilent) and NanoDrop 1000 Spectrophotometer (Thermo Scientific).
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bioRxiv - Genomics 2021Quote: ... and the quality was assessed using an Agilent 2100 Bioanalyzer with a High Sensitivity DNA analysis kit (Agilent, 5067-4626).
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bioRxiv - Genomics 2021Quote: ... The concentration and size distribution of the immunoprecipitated DNA was measured using the Bioanalyzer High Sensitivity DNA kit (#5067-4626, Agilent). Dana-Farber Cancer Institute Molecular Biology Core Facilities prepared libraries from 2 ng of DNA ...
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bioRxiv - Immunology 2021Quote: ... RNA integrity was assessed using Eukaryote Total RNA Nano Kit on the Bioanalyzer 2100 system (Agilent Technologies, Santa Clara CA). 500 ng of RNA ...
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bioRxiv - Developmental Biology 2021Quote: ... The quality of the extracted RNA was analysed using the RNA 6000 Nano kit on a 2100 Bioanalyzer (Agilent, USA). An aliquot of 500 ng of RNA was used to prepare a polyadenylated RNA library with the TruSeq Stranded mRNA Library Prep Kit (Illumina ...
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bioRxiv - Immunology 2021Quote: ... Total RNA was amplified and Cy3 labeled by using the one-color Low Input Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... with an average fragment length of 625 bp (ranging from 300 to 2000 bp, measured with the Bioanalyzer High Sensitivity DNA Kit, Agilent). The prepared library was sequenced on an Illumina NovaSeq 6000 Sequencing System with S4 flow cells ...
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bioRxiv - Microbiology 2021Quote: ... and Spir-1 and K7 plasmids were used as templates for site-directed mutagenesis according to the instructions of the QuikChange Site-Directed Mutagenesis Kit (Agilent). All mutations were confirmed by DNA sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were reselected for two rounds and finally transferred using a new capillary with 30-μm tip diameter to 100-μl lysis buffer (Absolutely RNA Nanoprep Kit; Agilent). Cells of multiple collections were pooled if needed to obtain 200 cells per sample ...
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bioRxiv - Microbiology 2021Quote: ... Mutant SARS-CoV-2 expression plasmids were generated by site-directed mutagenesis using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent). Unless otherwise stated all SARS-CoV-2 spike expression plasmids were based on the Wuhan-hu-1 reference sequence 41 ...
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bioRxiv - Neuroscience 2020Quote: ... The final product was assessed for its size distribution and concentration using a BioAnalyzer High Sensitivity DNA Kit (Agilent Technologies) and loaded onto an S1 flow cell on an Illumina NovaSeq 6000 (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... The average length of the final libraries was quantified using a Fragment Analyzer (HS NGS Fragment kit, Agilent, DNF-474) and the concentration of libraries was determined using a Qubit 1X dsDNA HS kit (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: Total extracted RNA quality was confirmed for sequencing using the Agilent 2100 Bioanalyzer system using the RNA 6000 Pico Kit (Agilent). Bacterial and mammalian rRNA depletion ...
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bioRxiv - Biochemistry 2020Quote: ... an XhoI restriction site was introduced after DrBphP residue 513 with QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies). Then ...
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bioRxiv - Systems Biology 2021Quote: ... The staining was performed according to the manufacturer’s procedure with EnVision G|2 Doublestain System Rabbit/Mouse (DAB+/Permanent Red) kit (Dako/Agilent K5361) on the Dako Autostainer ...
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bioRxiv - Biochemistry 2021Quote: ... Library size and quality was assessed using the Fragment Analyzer DNF-474 High Sensitivity NGS Fragment Analysis kit (Agilent Technologies). Concentrations of each library were quantified using the KAPA Library Quantification Kit for Illumina (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... The catalytically dead mutants MMP28EA-pCS2 and MMP28EA-ΔSP/NLS-GFP-pCS2 were produced by point mutation of Glutamic acid226 to Alanine from MMP28wtpCS2 and Glutamic acid209 to Alanine from MMP28ΔSP/NLS-GFP-pCS2 using QuikChange II site-Directed Mutagenesis Kit (Agilent #200523) with the primers MMP28_E-A_fdw ...
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bioRxiv - Biochemistry 2020Quote: ... fulgidus Csx3 (Supplementary figure 1) were generated using the QuikChange Site-Directed Mutagenesis kit as per manufacturer’s instructions (Agilent Technologies) and purified as for the wild-type protein.
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bioRxiv - Biochemistry 2020Quote: Site-directed mutagenesis of BAK1 scFv coding sequence into pCANTAB6 was performed following the manufacturer’s instructions in the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies). The mutations introduced in the BAK1 or the BAK1-INS1 template sequences were ...
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bioRxiv - Biochemistry 2020Quote: ... The error-prone PCR (epPCR) library of the gene encoding qmLC/A was created with the GeneMorph II kit (Agilent) according to the manufacturer’s instructions ...