Labshake search
Citations for Agilent :
5051 - 5100 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... We characterized a 1/10 dilution of both spikes using the TapeStation RNA HS kit (Agilent Technologies, CA, USA) and compared the true size distribution to the theoretical one (as calculated using the manufacturer’s molarity and sequence files).
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bioRxiv - Physiology 2024Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA).
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bioRxiv - Neuroscience 2024Quote: ... The resulting sequencing libraries were assessed for quality using the BioAnalyzer 2100 with a High Sensitivity DNA kit (Agilent), and quantified using the KAPA Library Quantification Kit ...
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bioRxiv - Genomics 2024Quote: ... The libraries were quality controlled using a 5200 Fragment Analyzer System (Agilent, HS NGS Fragment Kit, DNF-474-1000) and sequenced on a NovaSeq 6000/X instrument following 10X Genomics’ user guide recommendations.
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bioRxiv - Physiology 2024Quote: ... RNA quality was determined using the Agilent RNA 6000 Nano Kit (5067-1511, Agilent Technologies, Santa Clara, CA, USA) and chips were read using the 2100 software and a Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: Quality control on intermediate products and final libraries was performed using the Agilent Bioanalyzer High Sensitivity Kit (Agilent Technologies). The final libraries were quantified via qPCR using KAPA Library Quantification Kits (KAPA Biosystems ...
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bioRxiv - Biophysics 2024Quote: ... Site directed mutagenesis was performed with the help of Quick-change Site-Directed Mutagenesis kit (Stratagene, Agilent Technologies,USA) to generate several single tryptophan (W ...
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bioRxiv - Genomics 2024Quote: ... and RNA quality was checked using Agilent 2100 Bioanalyzer with an RNA 6000 nano kit (Cat# 5067-1511, Agilent).
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bioRxiv - Biophysics 2024Quote: ... G300D) were made using the QuikChange II XL kit using primers designed by the Agilent Primer Design Program (Agilent). The template for mutagenesis was pcDNA3 MYC-tagged WT Kir2.1 previously described34 ...
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bioRxiv - Systems Biology 2024Quote: ... mRNA libraries were also assessed for size distribution and adapter dimer presence (<0.5%) by the High Sensitivity Small Fragment DNF-477 Kit on a 96-channel Fragment Analyzer (Agilent).
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bioRxiv - Biophysics 2024Quote: ... Site directed mutagenesis was performed with the help of Quick-change Site-Directed Mutagenesis kit (Stratagene, Agilent Technologies,USA) to generate several single tryptophan (W ...
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bioRxiv - Systems Biology 2024Quote: ... and removal of rRNA was checked using Agilent RNA 6000 Pico Kit and an Agilent 2100 Bioanalyzer (Agilent Technologies). cDNA libraries were prepared with TruSeq Stranded mRNA Library Prep Kit (Illumina) ...
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bioRxiv - Cancer Biology 2024Quote: ... Quality controls for amplified cDNA libraries and final sequencing libraries were performed using Bioanalyzer High Sensitivity DNA Kit (Agilent). The sequencing libraries for scRNAseq were normalized to 4nM concentration and pooled ...
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bioRxiv - Cell Biology 2024Quote: ... Point mutations and deletions in the pDONR of interest were generated using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent), as described by the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... metabolic capacity and dependency analysis were performed in treated cells using the XF Mito Fuel Flex Test Kit (Agilent). All results were normalized to the number of cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Each sample’s quality and concentration were assessed with a Nanodrop and the Agilent RNA 6000 Nano Kit (Agilent Technologies). Only samples with a concentration of at least 50 ng/µl and an RNA Integrity Number (RIN ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyser (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyser (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB #E7770 ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse genomic libraries were generated using the SureSelectXT kit specific for the Illumina HiSeq instrument (Catalog No. G9611B; Agilent), followed by exome enrichment using the SureSelectXT Mouse All Exon bait set (Catalog No ...
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bioRxiv - Genetics 2024Quote: ... The H3.1K27M and H3.3K27M mutations were introduced by site-directed mutagenesis using the QuikChange II XL kit (Agilent Technologies). Briefly ...
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bioRxiv - Genetics 2024Quote: ... and the integrity of RNA was assessed on a Agilent 2100 Bioanalyzer using the RNA 6000 Nano Kit (Agilent). RNA samples were sent to BGI Genomics for sequencing and trimming.
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bioRxiv - Cell Biology 2024Quote: ... All point mutations and amino acid deletions were incorporated into constructs using the QuikChange site-directed mutagenesis kit (Agilent). All constructs were expressed using either the pINDUCER20-C1-EGFP lentiviral vector (Crawley et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... Final libraries were quality-controlled using an Agilent Bioanlyzer instrument with Agilent High Sensitivity DNA Kit (Agilent, 5067-4626). The DNA concentration of each library was measured using KAPA Library Quantification qPCR Kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Exomic sequences from DNA samples were enriched using SureSelect Human All Exon V.6 Kit (Agilent Technologies #5190-8892) according to the manufacturerer protocol followed by sequencing on a Hiseq PE150 (Illumina ...
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bioRxiv - Plant Biology 2020Quote: ... RNA quality and concentration of each sample were checked using an Agilent RNA 6000 Nano Kit Bioanalyzer chip (Agilent Technologies). Five micrograms of total purified RNA was enough for the reverse transcription reactions using the SuperScript III First-Strand Synthesis System for RT-PCR (Life Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... Site-directed mutagenesis of the pCMV-S4 vector was performed using protocols and materials from QuickChange Lightning mutagenesis kit (Agilent). Nearly confluent monolayers of HEK293 cells in 12 well plates were transfected with either 0.5 μg of empty vector or 0.25 μg each of WT σ3 or σ3 K293T expression vector using 1.5 µl Lipofectamine 2000 according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: Genes encoding EnHD and CHD in pET24 expression plasmids were described previously.(18) Single residue substitutions were introduced using a Quikchange site-directed mutagenesis kit (Agilent). Genes encoding sequences with multiple substitutions were synthesized by GeneArt (ThermoFisher Scientific ...
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bioRxiv - Genetics 2020Quote: Plamids for overexpression of the Nrd1 CID variants R133G and R133D were obtained using QuikChange site-directed mutagenesis kit (Stratagene).
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bioRxiv - Cell Biology 2021Quote: ... Hsc70D10N was generated by PCR-based site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). Cyclin D1 KO was conducted by CRISPR/Cas9 genome editing (Xie et al. ...
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bioRxiv - Systems Biology 2020Quote: ... The resulting pure DNA and RNA fractions were quality assessed using agarose gel electrophoresis and Bioanalyser RNA 6000 Pico Kit (Agilent). Nucleic acid concentration was quantified using Qubit dsDNA HS Assay and Qubit RNA HS Assay Kit (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: The lyase deficient mutant of HMGA2 was generated by sequential site-directed mutagenesis (QuikChange II Site-Directed Mutagenesis Kit, Agilent) of the first three arginines in the third AT-Hook domain ...
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bioRxiv - Molecular Biology 2020Quote: ... plasmid by digestion with AscI and MIuI and mutagenesis for RTEL1K48R was performed using QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent). Cells were infected with lentiviruses expressing the indicated RTEL1 proteins and infected cells were selected with puromycin as previously described (Garzia et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples quantity (RNA Integrity Number = RIN) and quality were assessed using the Agilent RNA 6000 Pico Kit (Agilent #5067-1513) on the Bioanalyzer 2100 ...
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bioRxiv - Cell Biology 2020Quote: ... The pcDNA3-HA-Bbs5-S19A construct was prepared by mutating Ser19 of Bbs5 to alanine with pcDNA-HA-Bbs5-WT as template and site-directed mutagenesis kit (Stratagene). The primers were FWD1 (5′-CTTCGACGTGTCCGCACAACAGATGAAAAC-3′ ...
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bioRxiv - Cell Biology 2020Quote: ... Site directed mutagenesis was done using a QuikChange® Lightning Site Directed Mutagenesis kit (Agilent Technologies, Inc., Santa Clara, CA).
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bioRxiv - Cell Biology 2020Quote: Mito fuel flex test: Agilent Seahorse XF Mito Fuel Flex Test Kit was used as per the standard protocol by Agilent Seahorse (https://www.agilent.com/cs/library/usermanuals/public/XF_Mito_Fuel_Flex_Test_Kit_User_Guide%20old.pdf) ...
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bioRxiv - Cell Biology 2020Quote: ... mRNA were extracted and the quality of these samples was assessed (Agilent RNA 6000 Nano Kit Quick, Agilent Bioanalyzer 2100); RNA Integrity Number (RIN ...
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bioRxiv - Cell Biology 2020Quote: A library encoding ARHGAP36 isoform 2 mutants was created via error-prone PCR (epPCR) using the GeneMorph II Random Mutagenesis Kit (Agilent). To determine the optimal epPCR conditions for library generation ...
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bioRxiv - Cell Biology 2020Quote: ... pWZL-Hygro-HDAC4PAM-ER pCW-Hygro-HDAC4PAM were obtained by sub-cloning a mutagenized HDAC4 (QuikChange Site-Directed Mutagenesis Kit, Agilent) into the linearized empty backbones by a restriction-based approach ...
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bioRxiv - Cell Biology 2020Quote: ... pEGFP-C1-tubbyCT R332H and pEGFP-C1-tubbyCT Y343F were generated using QuikChange II XL Site-Directed mutagenesis kit (Stratagene, Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... RNA integrity was determined by bleach agarose gel electrophoresis (Aranda et al., 2012) and further analyzed on a Bioanalyzer 2100 using the RNA 6000 Nano LabChip Kit (Agilent Technologies Inc. ...
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bioRxiv - Immunology 2021Quote: ... and extracellular acidification rate (ECAR) were measured in MSCs using a Seahorse XF24 Extracellular Flux Analyzer and kits from Agilent Technologies (Santa Clara ...
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bioRxiv - Immunology 2021Quote: ... PCR products were ligated into pSCB-Amp/Kan using the StrataClone Blunt PCR Cloning Kit (Agilent Technologies, La Jolla, CA). The Spic promoter was cloned into pGL3-Basic (Promega ...
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bioRxiv - Genetics 2021Quote: ... Quality assessment and library size estimation of the individual libraries was done using an HS DNA kit (Agilent, #5067-4626) in a Bioanalyzer 2100 ...
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bioRxiv - Developmental Biology 2021Quote: ... or the Illumina TruSeq Stranded Total RNA Library Prep kit (reripes samples) and checked using a Fragment Analyzer System (Agilent) before multiplexing ...
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bioRxiv - Molecular Biology 2021Quote: ... after which quantification and qualification of purified band(s) were undertaken using the Agilent DNA 1000 kit and BioAnalyzer (Agilent). Illumina NGS was performed with 2×250 bp sequencing without fragmenting the amplicons ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA probes were synthetized by cloning the DNA amplified region in the Strataclone PCR Cloning Kit (240205-5, Agilent Technologies) (hoxd12a ...
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bioRxiv - Biophysics 2020Quote: ... by introducing a stop codon at position 475 (aa 233–474) using the QuikChange XL site-directed mutagenesis kit (Stratagene). STIM1 fragment 344-449 (CAD ...
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bioRxiv - Developmental Biology 2020Quote: ... Size distribution and concentration of the final products were then evaluated using the Bioanalyzer High Sensitivity DNA kit (Agilent Technologies). The libraries were pooled and diluted to 3 nM using 10 mM Tris-HCl ...
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bioRxiv - Genomics 2020Quote: ... and RNA 6000 Pico and RNA 6000 Nano kits –depending on the experiment– with a BioAnalyzer 2100 (Agilent Technologies Inc.). Libraries for each sample type were prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina) ...