Labshake search
Citations for Agilent :
4901 - 4950 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... the QuantSeq 3ʹ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) was automated on the NGS WorkStation (Agilent) at The Centre for Applied Genomics (TCAG) ...
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bioRxiv - Plant Biology 2022Quote: ... were introduced in the coding sequence of C4 with the Quick-Change Lightning Site-Directed Mutagenesis Kit (Agilent Technologies).
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bioRxiv - Evolutionary Biology 2022Quote: ... The resulting two vectors were then subjected to site-directed mutagenesis (QuickChange II XL Site-Directed Mutagenesis Kit; Agilent Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μM fluoro-carbonyl cyanide phenylhydrazone (FCCP) and 0.5μM/0.5μM rotenone/antimycin A (all XFp Cell Mito Stress Test Kit, Agilent Technologies, 103010-100) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: ... The resulting fragments were cloned into the StrataClone TA-cloning vector and transformed into StrataClone SoloPack competent cells according to the manufacturer’s protocol (StrataClone PCR Cloning Kit, Agilent), resulting in pFF-162 (SpoY) ...
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bioRxiv - Molecular Biology 2022Quote: ... Quality of the library was assessed by using a BioAnalyzer via the High Sensitivity DNA Kit (Agilent, #5067-4626) and TapeStation via High Sensitivity D100 Screen Tape System (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... and the size was determined by Bioanalyzer 2100 using Agilent High Sensitivity DNA Kit (Agilent Technologies GmbH, Waldbronn, Germany). The final library was sequenced on the Illumina MiSeq platform using v3 chemistry ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA quantity and purity were analyzed of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN > 7.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... the cdc14hm mutation (encoding 414QPRK➔414AARA substitutions) was generated with the QuikChange Lightning site-directed mutagenesis kit (210518, Agilent) using pJC347 as the template and confirmed by DNA sequencing ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 Mpro mutants were generated with QuikChange® II Site-Directed Mutagenesis Kit from Agilent (Catalog #200524), using plasmid pE-SUMO-Mpro as the template ...
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bioRxiv - Cell Biology 2022Quote: ... To investigate mitochondrial respiration and energetic phenotypes a Seahorse XFp Cell Mito Stress Test kit (103010-100, Agilent Technologies) was used ...
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The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germinationbioRxiv - Plant Biology 2024Quote: ... Cyanine 3-labelled cRNA was synthesized from 150 ng RNA using the Low Input Quick Amp Labeling Kit (Agilent) and predicated using RNeasy Mini Kit (QIAGEN ...
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bioRxiv - Immunology 2024Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... and the quality was assessed using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer (Agilent Technologies) or Qubit (Thermo Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... or 27 into methionine on histone H3.3A using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies #200521) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... then washed in PBS and exposed to the detection kit (EnVision + System-HRP Anti-Rabbit, Dako Denmark A/S) which included 3,3′-diaminobenzidine tetrahydrochloride as chromogen and counterstained with Harris hematoxylin ...
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bioRxiv - Systems Biology 2024Quote: ... targeted DNAs were captured and enriched by SureSelect XT HS Target Enrichment Kit ILM Hyb Module according to the manufacturer’s instructions (Agilent). Indexed libraries were quantitated by high sensitivity DNA ScreenTape assay for TapeStation (Agilent) ...
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bioRxiv - Neuroscience 2024Quote: ... libraries were visualized on an Agilent 2100 Bioanalyzer using Agilent High Sensitivity DNA kit (Agilent Technologies, Cat. # 5067-4626) and quantified using Qubit dsDNA HS DNA Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEB Next Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Fragment traces were determined with a 2100 Bioanalyzer using the Bioanalyzer High Sensitivity DNA kit (Agilent, cat.no.:5067-4626), concentrations were measured using a Qubit dsDNA HS Assay Kit (Thermofisher ...
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bioRxiv - Neuroscience 2024Quote: ... Point mutations of Tmem120a were generated using the QuikChange II XL Site Directed Mutagenesis Kit (Agilent, Cat. Nu. 200522) per the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Telomeric FISH using a Cy3-labelled PNA probe was performed according to the manufacturer’s instructions (Telomere PNA FISH Kit/Cy3, Dako), all other methods including the cell cultivation followed the detailed protocols available in Supplementary Data of Altmanová et al ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were assessed for purity and concentration on the Bioanalyzer using the High Sensitivity DNA Kit (Agilent Technologies). Samples were multiplexed and sequenced on the Illumina HiSeq 2500 system (Genome Technology Core ...
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bioRxiv - Microbiology 2024Quote: ... R80A and H71R) or pLRGatewayIRESeYFP-Vpr plasmids were synthesised using the QuickChange II-XL site-directed mutagenesis kit (Agilent) according to manufacturer’s instructions and primers listed in Table 2 ...
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bioRxiv - Cell Biology 2024Quote: ... To investigate mitochondrial respiration and energetic phenotypes a Seahorse XFp Cell Mito Stress Test kit (103010-100, Agilent Technologies) was used.
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR was performed using the 1-Step Brilliant II SYBR Green QRT-PCR Master Mix kit (Agilent Technologies) and the TaqPath 1-Step Multiplex Master Mix kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... gRNA-target-resistant constructs were generated by site directed mutagenesis using QuikChange II Site-directed mutagenesis kit (200521, Agilent) or Q5 Site-directed mutagenesis (E0554 ...
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bioRxiv - Biophysics 2024Quote: ... Mutagenesis of nCPEB4 phosphorylation sites was performed using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies, 210513), with oligonucleotides purchased from Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The concentration and quality of the isolated RNA was checked on an Agilent Bioanalyzer (Agilent RNA 6000 Nano Kit) at the Beijing Genomics Institute (BGI) ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA (260/280>1.8) was extracted using TRIZOL and converted to cDNA using the SensiFast cDNA synthesis kit (Agilent). qRT-PCR was performed against target genes and quantified using a CFX96 Real-Time System (Bio-Rad) ...
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bioRxiv - Genetics 2024Quote: ... the E171Q variant was introduced into an SCN5A expression plasmid (pCDNA3.1) using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent). HEK293T cells were grown at pH 7.4 in filtered sterile DMEM nutrient medium (Life Technologies) ...
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bioRxiv - Genomics 2024Quote: ... and the cfDNA quality was assessed by the Fragment Analyzer High Sensitivity DNA Kit (Agilent, Santa Clara, California, USA).
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bioRxiv - Immunology 2024Quote: ... Library concentration and fragment sizes were evaluated with the Agilent High Sensitivity DNA kit (Cat. No. 5067-4626; Agilent). Libraries were sequenced on an Illumina NovaSeq 6000 sequencer (2x150 pb ...
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bioRxiv - Biochemistry 2023Quote: The F32A mutants of WT and Δ20-ACA8 were created using a QuikChange site directed mutagenesis kit (Agilent technologies) and the following primers:
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... was used for the RNA quality control with a microfluidic chip specific for RNA (Agilent RNA 6000 Nano Kit). Then 1 μg of qualified RNA samples in RNase-free ddH2O was used for library preparation ...
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bioRxiv - Developmental Biology 2023Quote: ... was used to remove genomic DNA contamination before cDNA was synthesised using AffinityScript Multiple Temperature cDNA Synthesis Kit (Agilent) using Oligo DT ...
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bioRxiv - Genomics 2023Quote: ... and its quality was assessed using the Agilent Fragment analyzer 5200 with the DNA (50kb) kit (Agilent, DNF-467). Next ...
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bioRxiv - Biophysics 2024Quote: ... where residues I3 and I5 were replaced with alanine using the Quick-Change Lightning kit following the manufacturer’s instructions (Agilent). Following mutagenesis ...
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bioRxiv - Bioengineering 2024Quote: ... Error-prone PCR reactions were carried out following the protocol provided by GeneMorph II Random Mutagenesis Kit (200550, Agilent). Primers were designed to flank residue 1 to 119 of the binder ...
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bioRxiv - Immunology 2024Quote: ... RNA quantity and purity analysis was done using Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0 ...
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bioRxiv - Cancer Biology 2024Quote: Paraffin-embedded human HCC samples were cut into 3.5-µm thick tissue sections and processed for immunohistochemistry with the EnVision FLEX kit material (K800021-2, Agilent Dako) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Alanine mutagenesis of the Survivin sequence (pGST-Survivin) was carried out using the QuikChangeR Site-Directed Mutagenesis Kit (Stratagene). Plasmid encoding GFP-Survivin D70A/D71A (pGFP-Survivin D70A/D71A ...
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bioRxiv - Cell Biology 2024Quote: Oxygen Consumption Rates (OCR) were measured using the Seahorse XF Cell Mito Stress Test Kit (Agilent, Cat. 103708-100) with a Seahorse XFe96 analyzer (Agilent) ...
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bioRxiv - Cancer Biology 2024Quote: ... The CDKN1B Serine 10 to alanine mutant reporter was cloned using the Quikchange II site-directed mutagenesis kit (Agilent).
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bioRxiv - Genetics 2024Quote: ... Quality control for the libraries was performed using the Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626). Libraries were sequenced using the Illumina Novaseq 6000 system with 2 × 150 paired-end kits ...
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bioRxiv - Cancer Biology 2023Quote: ... 154 and 175 of Twist1 in the pMPB3 vector were performed using the QuikChange XL site-directed mutagenesis kit accorinding to the manufacturer’s instructions (Stratagene), using the following primers ...
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bioRxiv - Immunology 2022Quote: ... The dpy-7p::old-1(D321A, N326S)::GFP plasmid (pFD23) was created using a Site Directed Mutagenesis Kit (Agilent) to introduce mutations into pFD21 using primers old-1kinasemutF and old-1kinasemutR ...
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bioRxiv - Molecular Biology 2023Quote: ... megaprimers were synthesized and cloned into the TFAM-mScarlet_pWPXL plasmid following the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) large insertion protocol ...
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bioRxiv - Biochemistry 2023Quote: ... A plasmid containing an IDE E111Q mutant was constructed using a QuikChange site-directed mutagenesis kit (Agilent Technologies, USA) and the pProEx-IDE-wt plasmid ...