Labshake search
Citations for Agilent :
5001 - 5050 of 6604 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: AtCOSY mutants were generated according to the protocol described in the QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies) using plasmid pHis8-4b::AtCOSY as the template and the primer sequences in Supplementary Table 5 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The quantity and quality of extracted RNA samples were analyzed using RNA 6000 Nano kit on a bioanalyzer (Agilent). RNA samples with RNA integrity number (RIN)>7 were used for library construction using the 3′ mRNA-Seq Library Prep Kit FWD for Illumina (QuantSeq-LEXOGEN ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA) and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB ...
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bioRxiv - Physiology 2023Quote: ... followed by Vectastain Elite ABC HRP detection kit (Vector PK-6100) and detection with DAB (Agilent Technologies K346811-2). Cell death and DNA fragmentation were detected with a TUNEL In Situ Cell Death Detection Kit (Roche ...
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bioRxiv - Microbiology 2023Quote: ... The TEAD-binding defective HPV8 E6 K136N mutant was made using the QuikChange II site-directed mutagenesis kit (Agilent). Nuclear/cytoplasmic fractionation was performed using the previously published Rapid ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Amino acid deletions for ΔKPQ were generated by use of the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent, USA). PCR products were cloned into XL-10 Gold Ultracompetent cells ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were also assessed for size distribution and adapter dimer presence (<0.5%) by the High Sensitivity NGS Fragment 1-6000bp kit on a 96-channel Fragment Analyzer (Agilent). All sequencing libraries were then normalized on the MicroLab STAR (Hamilton) ...
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bioRxiv - Cell Biology 2024Quote: ... and qualitatively assessed with the High Sensitivity NGS Fragment 1-6000bp Kit on a 96-channel Fragment Analyzer (Agilent). Final libraries were normalized ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The final libraries were then quality-checked and quantified for multiplexing using the Bioanalyzer High-Sensitivity DNA kit (Agilent) and Qubit dsDNA HS Assay kit (Thermo Scientific) ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... The integrity of the RNA was determined using the TapeStation 4150 (High Sensitivity RNA kit, Agilent Technologies #5067-5579). Library preparation was performed by EMBL Genomics Core Facility using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760L) ...
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bioRxiv - Immunology 2024Quote: ... PCR fragments were purified with Qiagen MinElute PCR Purification Kit and library concentration and quality were determined by Agilent High Sensitive DNA Kit and TapeStation ...
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A humoral immune response to parasitoid wasps in Drosophila is regulated by JAK/STAT, NF-κB and GATAbioRxiv - Immunology 2024Quote: ... The library quality was assessed using a Bioanalyzer high sensitivity kit on an Agilent 2100 Bioanalyzer (Agilent #5067-4626). The libraries were sequenced at Source BioScience using Illumina NovaSeq 150bp paired-end reads.
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... The integrity of the RNA was determined using the TapeStation 4150 (High Sensitivity RNA kit, Agilent Technologies #5067-5579). Library preparation was performed by EMBL Genomics Core Facility using the TruSeq Stranded mRNA Library Preparation kit according to the manufacturer’s instructions (Illumina #20020594) ...
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bioRxiv - Molecular Biology 2024Quote: ... The Agilent QuikChange Primer Design Program and the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, USA) were utilized to incorporate point mutations into the coding sequence of human GAT1 ...
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bioRxiv - Genetics 2024Quote: ... These mutations were introduced into pJW1 by site-directed mutagenesis using the QuikChange II kit (Agilent Technologies, Cat #200523) to make pJW1-KR>DD and pJW1-KR>AA ...
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bioRxiv - Cell Biology 2024Quote: ... The Agilent SureSelect Strand Specific mRNA library kit was used as per the manufacturer’s instructions (Agilent, Santa Clara, CA). Library construction began with two rounds of polyA selection using oligo dT containing magnetic beads ...
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bioRxiv - Developmental Biology 2024Quote: ... Point mutations were introduced into sel-5 and dpy-23 sequences using the QuikChange Site-directed Mutagenesis kit (Agilent). Transcriptional reporter Ppgp-12::gfp was created by inserting 3410 bp of pgp-12 promoter upstream of GFP in pPD95.81 ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA samples were run on an Agilent 2100 bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent, Diegem, Belgium). The bioanalyzer electropherograms were analyzed by Agilent 2100 Expert Software to determine the RNA quantity and quality ...
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bioRxiv - Cell Biology 2024Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) and Rotenone/Antimycin A was measured using the mitochondrial stress test kit (Agilent, USA). The glycolytic activity or glycoPER in response to Rotenone/Antimycin A and 2-deoxy-D-glucose was measured using glycolytic rate assay (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... Blood T cell fractions were estimated using TcellExTRECT using exons from the corresponding capture kit (Agilent custom V2 Exome) with a median coverage threshold set to five.
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bioRxiv - Physiology 2024Quote: ... for 24h then incubated in the Seahorse XF media (+/- 0.25mM succinate) and processed using the Cell Mito Stress Test Kit (#103015 Agilent).
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bioRxiv - Cell Biology 2024Quote: ... Target enrichment was performed to construct the exome library using the Agilent SureSelect Human All Exon V6 kit (Agilent Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... MicroRNAs (miRs) were polyadenylated and then re-verse-transcribed using the miR 1st-Strand cDNA Synthesis Kit (Agilent Technologies) according to manufacturer’s protocol.
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bioRxiv - Physiology 2024Quote: ... and the quality of the libraries was checked with a 2100 Bioanalyzer with a high-sensitivity DNA kit (Agilent). Libraries were sequenced using a P3 flow cell on a NextSeq2000 in 100 bp paired-end mode (Illumina ...
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bioRxiv - Biophysics 2024Quote: ... The resulting WGBS libraries were evaluated on a 2100 Bioanalyzer using Agilent High-Sensitivity DNA Kit (Agilent #5067-4626) and quantified via qPCR using the KAPA Library Quantification Kit (KAPA #KK4824) ...
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bioRxiv - Neuroscience 2024Quote: ... Quality and concentration of extracted RNA was assessed using a Tapestation 2100 with the High Sensitivity RNA kit (Agilent) for samples extracted form sorted somata and using a 2100 Bioanalyzer with the RNA 6000 Pico kit (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... The assessment of RNA integrity number (RIN) was carried out using the Agilent bioanalyzer (Agilent RNA 6000 Nano Kit). RNA from synaptoneurosomes was isolated using the same method ...
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bioRxiv - Physiology 2024Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: Site-directed mutagenesis was performed with a QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent technologies, Inc., Santa Clara, CA) to generate AAV2 and AAVDJ using keratinocyte-targeting variants [10][12] in pXR2 (encoding AAV2 Rep-Cap ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcoded libraries were made using NEB Ultra II DNA Library Prep Kit for Illumina following manufacturer’s instructions and quantified by Agilent 2100 Bioanalyzer System ...
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bioRxiv - Molecular Biology 2024Quote: ... ECAR of cells was measured using the Seahorse XF Glycolysis Stress Test Kit (Agilent Technologies, Palo Alto, CA, USA) on an XFe96 extracellular flux analyzer (Seahorse Bioscience ...
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bioRxiv - Neuroscience 2024Quote: ... The RNA integrity number (RIN) was determined using the Agilent RNA 6000 Pico Kit (Agilent Technologies, Cat#5067-1513).
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bioRxiv - Bioengineering 2024Quote: ... samples were transported on dry ice and analyzed using the RNA Pico Kit on the 2100 Bioanalyzer system (Agilent), performed by Cambridge Genomic Services ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries were validated using the NGS Kit on an Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), quantified (Qubit 4.0 Fluorometer) ...
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bioRxiv - Genomics 2024Quote: ... and the fragment size was assessed using gDNA 165 Kb kit on a Femto Pulse System (Agilent Technologies, USA). All DNA samples achieved the recommended Genome Quality Number (GQN ...
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bioRxiv - Genomics 2024Quote: cDNA was made using total RNA extractions (details above) and Accuscript High Fidelity First Strand cDNA Synthesis Kit (Agilent). Two µL of cDNA were used for TaqMan SNP Genotyping (Thermo Fisher) ...
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bioRxiv - Immunology 2024Quote: The E91G mutation was introduced into the A2 MuPyV genome using the Quikchange II Site- directed mutagenesis kit (Agilent) as described 29.
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bioRxiv - Microbiology 2024Quote: ... automated electrophoresis was performed using the Agilent 4150 TapeStation system and RNA ScreenTape kit (Catalog number: 5067-5576, Agilent). One of the water triplicates had a low RNA Integrity Number equivalent (RINe ...
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bioRxiv - Immunology 2024Quote: ... Site-directed mutagenesis was done following the protocol and reagents from QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent 210519), and the following primers (IDT ...
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bioRxiv - Neuroscience 2024Quote: ... the library preparations were sequenced on an Illumina Hiseq Ten platform with 150 bp paired end module.The panel kit was customized from Agilent Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... The fragment size of the final libraries was determined using the Bioanalyzer High-Sensitivity DNA Kit (Agilent, CA, USA). Library concentration of the final libraries was determined using the Tape Station High Sensitivity D1000 ScreenTape (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... The quality and concentration of the RNA was assessed with Agilent RNA 6000 Pico Kit (Agilent, Cat#5067-1513) on Bioanalyser 2100 (Agilent) ...
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bioRxiv - Neuroscience 2024Quote: ... RNA integrity was assessed with 1µl of each mRNA-purified sample using RNA 6000 Pico kit (Agilent, 5067-1513) on an Agilent Bionalyzer with RNA Integrity Number (RIN ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... The individual libraries were assessed using a Qubit™ and the Agilent Bioanalyzer dsDNA High Sensitivity Kit (Agilent Technologies). We sequenced at 2*76 base-pairs on a NovaSeq 6000 (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... of SspH1 was generated in the pcDNA3::2xHA-SspH1 backbone using the Quikchange II site-directed mutagenesis kit according to the manufacturer’s protocol (Agilent). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... Two µL of each diluted sample were run using the HS Large Fragment 50kb Kit #DNF-464-0500 (Agilent) according to the manufacturer’s instructions on the 5200 Fragment Analyzer System # M5310AA ...
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bioRxiv - Physiology 2024Quote: ... Proline point mutations were made in the Nav1.5 alpha subunit with the QuikChange II site-directed mutagenesis kit (Agilent) and primers from Sigma-Aldrich ...