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Citations for Leica :
1601 - 1650 of 2407 citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Neurons were transduced with LV-Syn1-GFP at DIV 3 and were imaged at DIV 21 using an inverted spinning disk confocal microscope (Leica DMi8, 63x objective). During image recording the cells were maintained in a humidified atmosphere of 5% CO2 at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Subsequently the samples were immersed in 100% ethanol for 3×10 min and then critical point dried in an Automated Critical Point Dryer Leica EM CPD300 (Leica Microsystems, Vienna, Austria) with 18 steps of ethanol - CO2 exchange.
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2, Leica Microsystems, Buffalo Grove, IL) at −90°C for 20 hours ...
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bioRxiv - Neuroscience 2023Quote: Dual or triple channel STED imaging of Ib and Is AZs was carried out using a Leica SP8 confocal/STED 3× microscope (Leica Microsystems, Mannheim, Germany) with 100X ...
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bioRxiv - Cancer Biology 2023Quote: ... rehydrated and subsequently stained with hematoxylin and eosin (H&E) and appropriate antibodies (Supplementary Table 3) using the automated system BOND RX (Leica Biosystems, Newcastle, UK). Visualization was by the Bond Polymer Refine Detection kit (Leica Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... Larvae were placed into a petri dish filled with HL-3 on ice and imaged (camera: Leica DFC365 FX, microscope: Leica MZ10 F). Brains were stained against HRP ...
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bioRxiv - Genomics 2021Quote: ... AT2 cell purity was determined by NKX2-1 cytospin staining (1:100, Leica Biosystems, Cat # NCL-1-TTF1). Donor1 was 83% positive ...
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bioRxiv - Cancer Biology 2022Quote: ... Ly6G (clone 1A8, 2B Scientific BE0075-1, ER2 Leica, 1:60000), LTF (Thermofisher ...
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bioRxiv - Genetics 2021Quote: FFPE tissue sections were cut using a microtome and baked at 62°C for 20 minutes followed by haematoxylin and eosin (H&E) staining using a Leica Autostainer XL (Leica Microsystems, Wetzlar, Germany). Slides were mounted with DPX (distyrene ...
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bioRxiv - Molecular Biology 2020Quote: ... The nuclei were imaged using a Leica DM6 B microscope equipped with Leica DMC5400 camera or DM IL LED fluorescence microscope equipped with Leica DFC450 C (Leica Microsystems, Wetzlar, Germany) camera and the images were processed using ImageJ software version 1.53 (National Institutes of Health ...
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bioRxiv - Immunology 2021Quote: ... The organs were then embedded into paraffin wax using a HistoCore Arcadia C and H paraffin embedding station (Leica Biosystems, Buffalo Grove, IL). Each organ was sliced into 4 µm sheets using a RM2235 manual microtome (Leica Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were dissected and sectioned into 40 μm-thick coronal slices at −20°C in a cryostat (Leica CM1850, Leica Biosystems, IL, USA). Coronal slices were then thaw-mounted on glass slides ...
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bioRxiv - Neuroscience 2020Quote: ... Motoneurons were patched at room temperature (approx 21°C) using infrared-differential interference contrast (IR-DIC) optics on a DMLFSA microscope (Leica DMLFSA; Leica Microsystems). All lumbar motoneurons were selected from the lateral motor nuclei of segments L1-6 and all cervical cells were selected from the lateral motor nuclei of slices made from C4-8 ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were dissected and sectioned into 40 μm-thick coronal slices at −20°C in a cryostat (Leica CM1850, Leica Biosystems, IL, USA). Coronal slices were then thaw-mounted on glass slides ...
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bioRxiv - Microbiology 2021Quote: ... the excess of solution was manually blotted using a Whatman filter paper and then the grid was plunge-frozen into liquid ethane cooled down at - 174°C by liquid nitrogen in a Leica EM CPC cryo-plunger (Leica Microsystems, Wetzlar, Germany). Three grids were prepared per condition ...
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bioRxiv - Microbiology 2020Quote: ... While keeping the orientation succession the slices were further processed into paraffin blocks using the Leica ASP6025 tissue processor and Leica EG1150H+C tissue embedding centre (both Leica Biosystems, Nussloch, Germany). Three serial adjacent 2.5 μm thick sections were then cut with the Leica RM 2255 Microtome (Leica Biosystems ...
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bioRxiv - Pathology 2022Quote: ... Heat-induced epitope retrieval (HIER) was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems, Richmond, IL). Slides were treated in protease (Advanced Cell Diagnostics ...
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bioRxiv - Neuroscience 2022Quote: ... Cryoprotected spinal cords were embedded in optimal cutting temperature (OCT) compound and frozen at −80 °C until cryostat sectioning (Leica Lm 1850, Germany). Transverse lumbar spinal cord cryosections were collected in serial at 30-μm or 40-μm thickness ...
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bioRxiv - Pathology 2021Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 20 minutes in sodium citrate-based ER1 buffer (Leica Biosystems, Richmond, IL) before immunostaining ...
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bioRxiv - Pathology 2021Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems, Richmond, IL). The SARS-CoV-2 probe (cat ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were extracted and snap-frozen on dry ice and sectioned at -20°C in 15 µm-thick coronal slices using a cryostat (Leica CM 1860 UV) and mounted onto Colorfrost Plus glass slides (Fisher 1255017) ...
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bioRxiv - Microbiology 2021Quote: ... Polymerization of these epoxy blocks was performed at 70 °C and then they were sectioned with an untramicrotome (Leica microsistems Gmbh, Wein Austria). Very small small sections of 80 nm thickness were collected on a cupper grid of 200 mesh (pore size ...
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bioRxiv - Neuroscience 2019Quote: ... the neurons at the end of treatment were stained with JC-1probe solution at 37 °C for 20 min and observed under fluorescence microscope (DMi8, Leica Biosystems, Wetzlar, Germany). The mitochondria were also extracted by a mitochondrial extraction kit ...
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bioRxiv - Plant Biology 2019Quote: ... The abaxial side of the leaf samples was imaged using a 63×/1.2 water immersion objective lens (Leica C-APOCHROMAT 63×/1.2 water). FLIM experiments were performed using a Leica TCS SP8X confocal microscope equipped with TCSPC (time correlated single photon counting ...
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bioRxiv - Physiology 2019Quote: ... 5 mm of the mid-belly of lateral gastrocnemius muscle was cut into 10-μm thick frozen muscle cross-sections at −20°C with a cryostat (Leica, Wetzlar, CM1850, Germany). Because of the uneven thickness of the diaphragm ...
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bioRxiv - Cell Biology 2022Quote: ... Vials were loosely capped and transferred to the chamber of the freeze substitution machine that contained about ½ inch-deep bath of ethanol to improve thermal conductivity and was pre-cooled to -112°C (EM AFSII, Leica Microsystems, Wetzlar, Germany). Within about 10 minutes in the freeze substitution machine ...
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bioRxiv - Immunology 2022Quote: Cryo-fluorescence microscopy samples were transferred to the cryo-FIB-SEM microscope in liquid nitrogen using a Leica EM-VCM500 machine and a cryo-holder suitable for c-clipped grids (Leica Microsystems, Vienna, Austria). Prior to sample loading into the FIB-SEM microscope ...
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bioRxiv - Neuroscience 2023Quote: ... snap-frozen in powdered dry ice and stored at -80 °C until sectioned at 12 µm in the coronal plane with a Leica CM1860 UV cryostat (Leica Biosystems, Wetzlar, Germany). Sections corresponding to Atlas plates 20-26 of Paxinos (Bregma +1.34 to +0.62 mm ...
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bioRxiv - Genomics 2023Quote: ... and sliced into 13 µm slices using PTFE-coated microtome blades (Duraedge, 7223) at -10°C on a Cryostat CM1860 (Leica, 14-0491-46884). Liver sections were mounted on VWR Superfrost Plus slides (VWR ...
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bioRxiv - Systems Biology 2023Quote: ... the mouse brains were sectioned coronally into 40 μm-thick slices at −18 to −20 °C using a microtome (Leica Microsystems, Wetzlar, Germany). Floating brain sections (fixed in 4% PFA ...
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bioRxiv - Developmental Biology 2021Quote: ... when embryos were fixed by 4% PFA for ensuing WISH analysis or imaged using a Fluorescent stereoscopic microscope (LEICA M205 FCA). 10 pg of gsc mRNA and 10 pg of ndr2 mRNA were injected ...
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bioRxiv - Biochemistry 2020Quote: ... but the purified V2R-Gs-Nb35 complex was at a concentration of 4 mg/ml and the cryo-EM grids were prepared using a EM GP2 (Leica microsystems) plunge freezer with a 4s blotting time (100% humidity and 4°C).
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bioRxiv - Molecular Biology 2021Quote: ... The paraffin blocks transferred to room temperature and the blocks sections cut 4 mm thick in a rotatory microtome (Leica RM2135) and mounted in glass slides ...
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bioRxiv - Neuroscience 2021Quote: ... mouse brain hemispheres prefixed with 4% PFA for 48 hours were sectioned at 50 μm using a Vibratome (Leica Microsystems Inc). Immunostaining of free-floating sections was performed using the ABC kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... The brains were removed and post-fixed in PBS containing 4% paraformaldehyde over night before cutting 50μm thick coronal sections on a vibratome (VT 1000 S from Leica, Wetzlar, Germany). Floating sections were permeabilized with 2% Triton X-100 in PBS overnight and blocked with 2% normal goat serum (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were embedded in 4% low-melting agarose and coronal cortical slices were generated (300 µm) with a vibrating microtome (VTS1000 Leica Microsystems). Slices were next incubated for 60 minutes at 37°C in DMEM/F-12 imaging media without phenol red supplemented with 10% fetal bovine serum (FBS).
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bioRxiv - Neuroscience 2020Quote: Brains were embedded in agar (3-4% w/v dissolved in dH2O) before being cut into 50 μm coronal sections on a vibrating microtome (VT1000S; Leica Microsystems). Free-floating tissue sections were collected in series ...
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bioRxiv - Cell Biology 2021Quote: ... The livers were mounted in 4% low-melting agarose in PBS and cut into 100 μm – thick sections at a vibratome (Leica VT1200S). For deep tissue imaging ...
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bioRxiv - Neuroscience 2020Quote: ... Sections of 300μm thickness were embedded into 4% low melting agarose and further cut into 50 μm slices with vibrating-blade microtome (Leica, Germany). Nuclei were counterstained by adding a small drop of ProLong™ Gold Antifade Mountant with DAPI (Invitrogen™ ...
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bioRxiv - Neuroscience 2020Quote: Brain organoids (4.5 months old) were embedded into 4% low melting agarose and 300 μm slices were cut with vibrating-blade microtome (Leica, Germany). Slices were prepared using ice-cold artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Cell Biology 2022Quote: ... Cell layers were embedded in paraffin and 4 μm thick sections were made using a Leica RM2165 microtome (Leica, Wetzlar, Germany). Alcian Blue/Periodic Acid-Schiff (AB/PAS ...
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bioRxiv - Cell Biology 2022Quote: ... The imaged area was marked with a circle (4 mm in diameter) around the center of the imaged area using an objective diamond scriber (Leica, 11505059)39 ...
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bioRxiv - Neuroscience 2021Quote: Mouse brains were fixed in 4% paraformaldehyde and cut into 40-μm-thick sections using a vibratome (Leica Biosystems, Nussloch, Germany). The sections were blocked using blocking solution (X0909 ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were extracted from the skull and stored in 4% formaldehyde before being cut in sagittal sections (50 μm) using a vibratome (Leica VT1000) and stained with cresyl violet ...
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bioRxiv - Plant Biology 2020Quote: ... by infiltration for 10 min in a vacuum and after further incubation for 4 h processed using the Leica Semi-Enclosed Benchtop Tissue Processor TP1020 (Leica Biosystems) for an ethanol series (75 % ...
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bioRxiv - Bioengineering 2021Quote: ... according to standard histological procedures and 4 μm thick sections of the paraffin blocks were cut on the microtome (Leica, HM325). The paraffin sections were stained with Haematoxylin-Eosin (HE ...
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bioRxiv - Neuroscience 2021Quote: ... brains of perfused mice (n=4 per group) were serially cut into 35 μm-thick coronal sections using cryostat (Leica, Germany). Sections were permeabilized in PBS containing 0.3% Triton X-100 for 30 min ...
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bioRxiv - Neuroscience 2020Quote: Brains electroporated with the pCAG-mRFP;Hes5p-GFP plasmid were dissected 11 h after electroporation and embedded in 4% Agarose in complete HBSS medium (Polleux and Ghosh, 2002) and sectioned using a vibratome (Leica VT1200S). Electroporated areas of the lateral cortex were microdissected under the fluorescent dissecting scope (Zeiss Axio Zoom) ...
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bioRxiv - Immunology 2022Quote: ... grids were metalized by platinum sputtering (4 nm) using a Leica ACE600 cryo-sputter coater equipped with a cryo-stage (Leica Microsystems). Metallized samples were then transferred to a pre-cooled Crossbeam 550 FIB-SEM microscope (Carl Zeiss NTS GmbH ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post fixed in 4% paraformaldehyde overnight and cryoprotected in 20% sucrose azide prior to freezing and sectioning at 40μm (CM3050S; Leica, Wetzlar, Germany). Tissue slices containing M2 were stained with neutral red and electrode placement was validated ...