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Citations for Leica :
1551 - 1600 of 2407 citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... then transcardially perfused with 10% sucrose in phosphate buffered saline (PBS) followed by 4% paraformaldehyde in PBS for approximately 90 s each using a Perfusion Two system (Leica). The brain was then removed and postfixed in 4% paraformaldehyde in PBS overnight at 4°C before being moved to a 30% sucrose solution in PBS for cryoprotection ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples were embedded in gelatin/ albumin with 4% of glutaraldehyde and sectioned (20 μm) on a VT1000 S vibrating blade microtome (Leica). Sections were mounted in Fluoromount Aqueous Monting Medium (Sigma).
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bioRxiv - Neuroscience 2019Quote: ... The OE were embedded in 4% low melting point agarose blocks and sectioned (30 µm) using a Vibratome (VT 1200; Leica). The sections were blocked in 1% blocking solution (10 min at RT ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were cut coronally into 15-μm sections and mounted in 4 series using a cryostat (Leica CM1950, Nussloch, Germany) to obtain 16 matching sections containing the DR (−7.3 to −8.7 mm from bregma ...
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bioRxiv - Plant Biology 2019Quote: ... Nodules were embedded in 4% agarose and 50-100 μM sections were made with a vibratome® 1000 plus (Leica). Sections were immersed in a staining solution (2.5% 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic (X-Gluc) ...
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bioRxiv - Neuroscience 2019Quote: ... 2.5 mM KCl, 25 mM glucose, 1.25 mM NaH2PO4, 4 mM MgCl2, 0.5 mM CaCl2, and 24 mM NaHCO3, Leica VT1200 vibratome). The slices were incubated at 32 °C for 60 minutes after sectioning and were then stored at room temperature until they were used ...
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bioRxiv - Neuroscience 2021Quote: ... Striatum containing hNSCs (equivalent to +1.4 to +0.14 mm from Bregma) (Franklin and Paxinos, 2007) was cut at 60 μm using a vibratome (Leica Microsystems). After pre-embed IHC of the striatum using diaminobenzidine (DAB ...
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bioRxiv - Developmental Biology 2021Quote: ... Fixed heads were embedded in 4% low gelling agarose and 200 µm sections were obtained using a vibratome (Leica, VT1200S). Sections were post-fixed with 1% osmium tetroxide in water ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... with an Apochromat 63 x 1.4 NA oil-immersion objective and a charge-coupled device camera (ORCA-R2; Hamamatsu Photonics) using LAS-AF software (Leica). Puncta were counted manually using ImageJ.
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bioRxiv - Neuroscience 2022Quote: ... Frozen brains were cut to either 10 μm slices for immunofluorescence staining or 4-6 μm for STORM (CM1950, Leica) to produce coronal brain sections.
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bioRxiv - Neuroscience 2022Quote: ... The paraffin embedded sections were then sliced into 4 μm-thick slices on a Leica RM2016 Microtome (Leica, Shanghai, China) for immunohistochemistry staining and apoptotic cells labeling ...
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bioRxiv - Neuroscience 2023Quote: ... and brains were postfixed in 4% paraformaldehyde and preserved with 30% sucrose before sectioning at 40-µm on a freezing-sliding microtome (SM2010R, Leica). Sections were incubated overnight with indicated primary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were embedded in 4% agarose before being cut into 40 um coronal slices with a vibratome (Leica VT S10000) and stored in 0.1% PBS Sodium Azide ...
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bioRxiv - Neuroscience 2022Quote: ... Fixed brain slices were washed once with 1x PBS pH 7.4 and subsequently submerged in 30% (w/v) sucrose for two days and then embedded in tissue freezing medium (Leica Microsystems ...
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bioRxiv - Neuroscience 2022Quote: ... larvae were embedded in 4% agarose in PBS and 50-100 μm sections were obtained using a vibratome (Leica, VT1200S). Sections were counterstained with DAPI (Thermo Fisher Scientific Cat#62248 ...
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bioRxiv - Neuroscience 2023Quote: ... Cell aggregates and transplanted retinas harvested from RD-nude rats were fixed with 4% paraformaldehyde (Wako) and sectioned with a cryostat (Leica) to prepare frozen sections ...
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bioRxiv - Neuroscience 2023Quote: ... for 50-μm sections, the brains were embedded in 4% agar (01059-85, Nacalai Tesque) and sliced using a vibratome (VT1200S, Leica). For immunohistochemistry ...
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bioRxiv - Genomics 2023Quote: ... each liver sample was exhaustively sectioned into 4 μm-thick sections by a fully automated rotary microtome (Leica RM2255, Germany). Each of these sections was stained with hematoxylin and eosin and mounted ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were then coated with a 4 nm layer of platinum using a ACE600 high resolution sputter coater (Leica Microsystems) before being imaged using an FEI Quanta 450 ESEM (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... with an Apochromat 63 x 1.4 NA oil-immersion objective and a charge-coupled device camera (ORCA-R2; Hamamatsu Photonics) using LAS-AF software (Leica). Puncta were counted manually using ImageJ.
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bioRxiv - Molecular Biology 2023Quote: ... Immunohistochemistry or immunofluorescence experiments were performed on 4 µm-sections using Novolink polymer detection system (7140-K, Leica Micro-systems) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Processed tissues were embedded in paraffin and sectioned at 4 microns on a rotary microtome (Leica Biosystems, Buffalo Grove, IL). Tissues were mounted on glass slides and stained with hematoxylin and eosin using routine protocols on an automated histostainer (Leica ST5010 Autostainer ...
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bioRxiv - Genomics 2023Quote: ... each liver sample was exhaustively sectioned into 4 μm-thick sections by a fully automated rotary microtome (Leica RM2255, Germany). Each of these sections was stained with hematoxylin and eosin and mounted ...
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bioRxiv - Developmental Biology 2023Quote: ... Undecalcified frozen sections at a thickness of 4 µm were obtained according to Kawamoto’s film method using TC-65 (Leica Microsystems) or SL-T35 (UF ...
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bioRxiv - Genomics 2024Quote: ... Sections of 4 μm without wax were stained with safranin and fast green and photographed with electric inverted fluorescence microscope (MDi8, Leica).
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bioRxiv - Neuroscience 2024Quote: ... The brains were kept in 4% PFA at least overnight and stored in PBS until histological slicing (Leica VT1200 S). DAPI-Fluoromount-G (70-100 µm slices ...
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bioRxiv - Neuroscience 2024Quote: ... Cortices were included in 4% agar and tangential sections were cut at 100 µm on a vibrating microtome (Leica, VT1000S).
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bioRxiv - Cancer Biology 2024Quote: ... Cryo-embedded mouse tumors (OCT compound, Tissue-Tek, #14291) were cut to 4-5µm with a cryostat (Leica CM 1950, Leica Biosystems) and positioned onto SuperFrost plus slides (R.Langenbrinck ...
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bioRxiv - Neuroscience 2024Quote: ... electroporated cerebella were embedded in 4% LMP agarose dissolved in mHBSS and then cut into 300 mm sagittal slices using a VT1200 vibratome (Leica). The slices were finally placed on 0.4 mm PTFE cell culture inserts (Millicell ...
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bioRxiv - Microbiology 2023Quote: ... TTF-1 (Leica), IBA1 (Fujifilm) ...
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bioRxiv - Molecular Biology 2019Quote: ... To increase contrast the grids were stained with 2% aqueous uranyl acetate and lead citrate (Leica). The grids were examined using a Philips CM 100 Compustage (FEI ...
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bioRxiv - Cancer Biology 2020Quote: ... shRalA and shRalB cells with a Nanoject microinjector 2 (Drummond) under a M205 FA stereomicroscope (Leica), using micro-forged glass capillaries (25 to 30 mm inner diameter ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were first permeabilized with heat in Bond Epitope Retrieval solution 2 (pH 9.0, Leica - AR9640) at 95°C for 2 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Heat induced antigen retrieval was performed at pH 9 (Bond Epitope Retrieval solution 2, Leica Biosystems), 100°C for 30mins ...
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bioRxiv - Microbiology 2021Quote: ... They were then freeze substituted in a EM AFS 2 system (Leica Microsystems GmbH, Wetzlar, Germany) and embedded into Epon resin ...
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bioRxiv - Neuroscience 2020Quote: ... (2) The SHG signal was collected from above the retina through a x40 0.8 NA (Leica) objective ...
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bioRxiv - Cancer Biology 2020Quote: ... At least 2× step sections per lung were stained on a Bond Automated Stainer (Leica Biosystems) using standard protocol F and Target Retrieval Solution pH 6.0 (Dako ...
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bioRxiv - Plant Biology 2020Quote: ... leaf discs were collected 2 dpi and observed using a Leica TCS SP8 (Leica, Mannheim, Germany) confocal microscope with the following excitation wavelengths ...
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bioRxiv - Microbiology 2019Quote: ... 2) the original bright-field condenser was replaced with an air objective (40× NA = 0.6, Leica); and 3 ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were mounted in 2% agarose and sectioned at 50μm using a vibratome (Leica VT1000s, Germany). Coronal sections were used for all brains ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were mounted in 2% agarose and sectioned at 50 μm using a vibratome (Leica VT1000s). Coronal sections were used for all brains ...
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bioRxiv - Neuroscience 2023Quote: ... embedded in 2% agarose and sectioned to 50-μm coronal/sagittal sections by a vibratome (Leica). For OE sections ...
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bioRxiv - Cancer Biology 2023Quote: ... FFPE tissue sections (2 μm thickness) obtained with a Leica RM2245 rotary micro-tome (Leica Biosystems) were mounted on glass slides (Superfrost Plus ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen was unmasked with Tris-EDTA pH 9 (Bond Epitop Retrival Solution 2 Leica, cat# AR9640). Both mouse monoclonal anti-Ki67 CloneMIB-1 (Dako ...
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bioRxiv - Cancer Biology 2024Quote: ... unstained sections were heated for 30 minutes in BondTM Epitope Retrieval Solution 2 (No. AR9640; Leica Biosystems Newcastle LTD ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue blocks were then embedded in 3% agarose gel and cut in the sagittal plane at 30 μm-thickness by the vibratome (Leica, Buffalo Grove, IL), counter-stained with Dapi (1:2000 dilution ...
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bioRxiv - Cancer Biology 2020Quote: Super-Resolution Stimulated Emission Detection (STED) microscopy (Figure 1A, Figure S1, Figure 4A) was performed using a commercial Leica SP8 STED 3× system (Leica Microsystems, Mannheim, Germany), equipped with a white light laser with continuous spectral output between the wavelengths of 470 nm to 670 nm ...
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bioRxiv - Neuroscience 2020Quote: ... whereas slices processed for immunofluorescence were embedded in 3% agarose solution and re-sliced at 40-µm thickness using a vibratome (Leica VT1200S; Leica Microsystems; Bannockburn, IL).
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bioRxiv - Neuroscience 2020Quote: ... the brains were embedded in 3% agar and cut into 40 μm coronal visual cortical sections using a vibratome (Leica Biosystems, Wetzlar, Germany). After washing with PBST (1x PBS and 0.2% TritonX100) ...
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bioRxiv - Plant Biology 2021Quote: ... Cells cultured in M9 or LS medium containing 3% sucrose were added to flat specimen carriers and frozen using a high-pressure freezing machine (Leica EM PACT, Leica Microsystems, Wetzlar, Germany). The frozen samples were transferred to 2% osmium tetroxide in anhydrous acetone at -80 °C and incubated at -80 °C for 6 days (120 h) ...