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Citations for Leica :
1451 - 1500 of 2407 citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and CD8 alpha antigen retrieval was performed with BOND Epitope Retrieval 2 – ER2 (AR9640, Leica) for 40 ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μm z-steps) were taken using a laser scanning confocal microscope (TCS SP8, Leica). Three sections of 20 μm depth each were averaged per animal for analysis ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides underwent heat-induced epitope retrieval with Epitope Retrieval Solution 2 (pH 9.0, Leica AR9640) at 95°C for 5 mins ...
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... aortas were placed between 2 clear sheets and imaged by M205 FA stereoscope system (Leica).
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bioRxiv - Cell Biology 2020Quote: ... L4 animals were fixed by high-pressure freezing with the EMPACT-2 system (Leica Microsystems). Freeze-substitution (FS ...
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bioRxiv - Cancer Biology 2019Quote: ... Slides for CD3 stain were retrieved for 30 minutes using Epitope Retrieval 2 (EDTA; Leica) and incubated in protein block (Rodent Block M ...
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bioRxiv - Immunology 2021Quote: ... samples underwent heat induced antigen retrieval (HIER) in BOND Epitope Retrieval 2 (AR9640, Leica Biosystems) or BOND Epitope Retrieval 1 (AR9961 ...
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bioRxiv - Plant Biology 2023Quote: ... and images were taken every 20 min for 2 days using microscope (LEICA M205 FA). Imaging data were analyzed with ImagJ (FIJI).
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bioRxiv - Bioengineering 2022Quote: ... and the appropriate visualization system was applied – DAKO DAB (Cat# K346811-2, Leica, Heidelberg, Germany) for all but CD206 which utilized AEC (Cat# K400511-2 ...
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bioRxiv - Cell Biology 2023Quote: Freeze substitution was performed in a Leica EM AFS-2 freeze substitution unit (Leica Microsystems) as described previously (Bragulat-Teixidor et al. ...
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bioRxiv - Immunology 2024Quote: ... Heating mediated-epitope/antigen retrieval was performed with Bond TM Epitope Retrieval 2 (Leica Biosystems). Blocking was performed with plant-based protein blocking buffer (Cell Signaling Technology [CST] ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by an initial antigen retrieval using Epitope retrieval solution 2 (Leica Bond ER2, #AR9640) at 95°C for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... brains were then transferred to a 30 % sucrose solution for at least 3 days and coronal sections (200 µm) containing hippocampus were obtained using a vibratome (Leica vt 1000S). Sections were collected into 24-wells plate in sucrose 6% for one day stored at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... containing dorsal hippocampus (from 3, 6, 9, 12, 15, 19, and 24 month-old) were cut from PFA-fixed hemispheres (Leica vibratome VT1000P) and stored in 0.1M phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: ... The embedded tissues were sectioned to 3 μm-thick sections and stained with Haematoxylin-Erythrosin-Saffron (HES) before visualizing under a Leica DMRBE light microscope (Leica Microsystems, Germany). The stained sections were scanned with a Hamamatzu NanoZomer S60 ...
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bioRxiv - Plant Biology 2022Quote: ... 1 cm segments from the root tip were embedded in 3% agarose and were sectioned at 150-200 μM using a vibratome (Leica VT1000 S). The sections were stained in Clearsee buffer (Ursache et al ...
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bioRxiv - Cancer Biology 2021Quote: ... endogenous peroxidase activity was blocked by incubation with 3% peroxide block for 20 minutes (BOND Polymer Refine Detection System, Leica, Germany; DS9800). The sections were blocked with 10% normal goat serum (Vector ...
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bioRxiv - Cancer Biology 2019Quote: ... Larvae were washed 3 times with PBS 1x and livers were carefully dissected with the use of forceps on a stereomicroscope (Leica MZ 9.5). After dissection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... At least 3 pictures/section were acquired at 40x and 63x magnification by DMI600 wide field fluorescence microscope (Leica microsystems, Wetzlar, Germany). Hoechst signal (cell nucleus ...
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bioRxiv - Plant Biology 2022Quote: ... The roots were washed in water and one cm segments from the root tip were embedded in 3% agarose and made sections of 150-200 μM using a vibratome (Leica VT1000 S). Confocal Laser Scanning microscopy was performed on a Zeiss LSM700 confocal with the 20X objective ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were rinsed in 0.1 M phosphate buffer (PB; 3 × 10 min) and 50-70 μm-thick coronal sections were obtained with a vibrating blade microtome (Leica VT1000 S).
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bioRxiv - Developmental Biology 2024Quote: ... Brains were embedded in 3% low-melting point (LMP) agarose and sectioned coronally at 100 μm using a vibratome (Leica, model VT1200). Free-floating sections were incubated in block solution (2% donkey serum ...
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bioRxiv - Neuroscience 2023Quote: ... Larvae were placed into a petri dish filled with HL-3 on ice and imaged (camera: Leica DFC365 FX, microscope: Leica MZ10 F). Brains were stained against HRP ...
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bioRxiv - Neuroscience 2024Quote: Paraffin-embedded fixed postmortem human brain tissues (from 34 AD patients and 30 NCs) were cut into 3 µm sections using a Leica microtome (Leica Microsystems, Germany) (46 ...
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bioRxiv - Cancer Biology 2024Quote: ... Permeabilized cells were probed for SMAD2/3 (#AF3797, R&D Biosystems, 10μg/mL) and imaged on an SP8 white laser confocal microscope (Leica Biosystems, Wetzlar, Germany). The following antibodies were used for additional immunofluorescence assays ...
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bioRxiv - Neuroscience 2020Quote: ... Coronal sections of the whole SN of Young 1 and Old 1 were obtained by sectioning the tissue (20 μm) on a freezing microtome (Leica, CM1850UV-1-1, Germany) at −24 °C.
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bioRxiv - Cancer Biology 2020Quote: ... CD21 (DAKO, 1:25, CD23 (Leica, CD23-1B12, 1:50), CD4 (DAKO ...
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bioRxiv - Neuroscience 2023Quote: ... PV Poly-HRP Anti-Mouse IgG (Leica, PV6119, 1:1), ImmPRESS®-AP Horse Anti-Rabbit IgG Polymer (Vector ...
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bioRxiv - Cancer Biology 2023Quote: ... and Ly6G (2B Scientific BE0075-1, 1:60000, ER2 Leica). Nkp46 and FoxP3 were stained on a Dako AutostainerLink48 ...
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bioRxiv - Biophysics 2020Quote: ... the hind wings are dehydrated and cut into six pieces at the nanoindentation test points at the C+ScA after being embedded in paraffin using a paraffin embedding machine (Leica EG1150C, Leica Biosystems, Germany) to support the specimens in the slicing test ...
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bioRxiv - Cell Biology 2020Quote: Frozen 10-μm thick tissue cross-sections were cut from the mid-belly of the two lobes each sample at −20 °C with a cryostat (Leica, CM1950, Germany) and then stored at −80 °C for further staining ...
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bioRxiv - Developmental Biology 2021Quote: ... Gelatine blocks were flash frozen at −80 °C in pre-cooled heptane and sectioned in 25 μm slices in a cryostat (Leica, CM3050 S). The slides dried overnight at room temperature and were mounted with mowiol and sealed with nail polish prior to confocal microscopy.
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bioRxiv - Biophysics 2020Quote: ... The blotted samples were immediately plunged into liquid ethane at its freezing point (−183 °C) using an automatic plunge freezer (Leica, EM GP). The vitrified specimens were then transferred into liquid nitrogen for storage ...
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bioRxiv - Microbiology 2021Quote: ... pallidum strain Chicago C was thawed at room temperature and bacteria were enumerated on a Leica DM2500 darkfield microscope (Leica, Wetzlar, Germany) to inoculate 106 bacteria into each well of the 6-well plate ...
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bioRxiv - Neuroscience 2019Quote: ... The slides were kept in a moist chamber (37°C) for subsequent incubation periods and further visualized with 63X oil immersion objective under a DMi8 microscope (Leica, Wetzlar, Germany) in the DIC mode.
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bioRxiv - Cell Biology 2021Quote: ... 10 µm thick sections covering the injured area were produced using a cryostat maintained at -20°C (Leica CM 3050s, Techno-med). To assess the integrity of mitochondrial function the sections were sequentially stained for COX and SDH activities ...
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bioRxiv - Plant Biology 2020Quote: ... The number of protoplasts was counted with the C-Chip Neubauer improved hemocytometer (NanoEnTek, Seoul) under a light microscope (Leica VT1000, Wetzlar). The viability of protoplasts was determined using trypan blue solution (Gibco ...
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bioRxiv - Physiology 2022Quote: ... embedded in Tissue Tek® and after freezing at −80°C cut in 10 μm cross-sections using a Cryostat Microm HM560 (Leica Biosystems).
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bioRxiv - Cell Biology 2022Quote: ... The frozen sample were transferred to cryovials in liquid nitrogen vapor and transferred to a pre-cooled (−90°C) freeze substitution unit (Leica EM AFS). Freeze substitution was performed using a mixture of 0.12% aqueous Uranyl acetate in anhydrous acetone using the following program ...
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bioRxiv - Bioengineering 2022Quote: ... frozen human brains were sliced at a temperature of −30 °C into 60 μm-deep coronal sections using a cryostat microtome (Leica Microsystems, Germany). Finally ...
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bioRxiv - Biochemistry 2021Quote: ... Mouse brain tissue sections were sectioned at −21°C to a 12 μm thickness using a CM1850 Cryostat (Leica Microsystems, Wetzlar, Germany). Tissue sections were thaw mounted onto glass microscope slides (IMEB ...
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bioRxiv - Immunology 2021Quote: ... the chambered slide was imaged using Leica SP5 inverted confocal microscope with environmental chamber to maintain the sample humidified at 37°C (Leica Microsystems, USA). Sections were serially imaged over the course of 1-2 hours and compiled using LAS X software (Leica Microsystems ...
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bioRxiv - Cell Biology 2020Quote: ... The grids were manually blotted using Whatman filter paper and plunge-frozen into liquid ethane at −174°C using a Leica EM-CPC equipment (Leica, Wetzlar, Germany). After freezing ...
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bioRxiv - Biochemistry 2022Quote: ... 1 at 10 °C and maintained at 90% humidity and then plunge frozen into liquid ethane (−180 °C) using a Leica GP plunger (Leica Microsystems, Austria).
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bioRxiv - Physiology 2022Quote: ... Samples were embedded in the Optimal Cutting Temperature compound and sectioned to 20–30 µm at 50 °C using a cryostat (Leica CM 3050S) and stained with haematoxylin & eosin using Sakuras Tissue-Tek DRS 2000 at the Department of Anatomy ...
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bioRxiv - Pathology 2022Quote: ... Slides were dried in a 60 °C oven for 30 min and mounted with a permanent mounting medium (Micromount®, Leica Biosystems). Lung sections from a SARS-CoV-2-infected hamster were used as positive assay controls.
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bioRxiv - Molecular Biology 2023Quote: ... Vitreous sectioning was performed on infected cells tubes containing the vitrified sample pre-cooled at −140°C on an EM UC6/ FC7 cryo-ultramicrotome (Leica Microsystems, Vienna). The sample was trimmed to a pyramidal shaped block of 140 μm base and approximately 50 μm height using a 45° cryo trimming diamond knife (CT441 ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then frozen in methylbutane solution (−50 to −60 °C) and sectioned into 40 μm serial sections with a freezing microtome (Leica SM 2010R). Serial sections spaced 240 μm apart representing the entire forebrain were collected in a 6-well tray using a freezing microtome (Stoyka et al. ...
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bioRxiv - Microbiology 2022Quote: ... Grids were blotted for 5 s and plunge-frozen in liquid ethane cooled by liquid nitrogen to approximately -195 °C using an EM GP Plunge Freezer (Leica, Wetzlar, Germany).
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bioRxiv - Neuroscience 2023Quote: ... The brains were frozen using dry ice to preserve at - 80°C and sectioned at a thickness of 40 μm using a cryostat (CM3050S, Leica Biosystems, Germany).