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Citations for Leica :
1701 - 1750 of 2407 citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.05% uranyl acetate in acetone and placed into an AFS-2 Freeze Substitution Machine (Leica Microsystems, Austria). Samples were freeze-substituted at −90°C for 72 h ...
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bioRxiv - Physiology 2021Quote: ... CFP and DsRed fluorescence signal were detected after 2 days with a CLSM (Leica AF6000 modular microsystems). All experiments were biologically repeated for three times.
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bioRxiv - Neuroscience 2021Quote: ... or inferior colliculus were cut at 300 µm with a vibratome (VT1200S, Leica, or 7000smz-2, Campden) in warm aCSF ...
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bioRxiv - Neuroscience 2020Quote: ... Antigen retrieval was done by incubation with Bond Epitope Retrieval solution 2 (AR9640, Leica Biosystems, Wetzler, Germany) for 20 minutes and then sections were then washed with Bond Wash solution 3x ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 % glutaraldehyde and 0.25 % uranyl acetate for 60 h in a FS system (AFS-2, Leica Microsystems). Larvae were embedded in an Epon-Araldite mix (hard formula ...
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bioRxiv - Cancer Biology 2019Quote: ... Slides for CD14 and CD163 stains were retrieved for 20 minutes using Epitope Retrieval 2 (EDTA; Leica) incubated in protein block (Rodent Block M ...
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bioRxiv - Bioengineering 2022Quote: ... and counted for expression of opie-2-dsRed and 3xP3-tdTomato using a fluorescent stereoscope (Leica M165FC). The difference in total larval counts compared to total egg counts were considered to be dead during embryonic or early larval stages ...
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bioRxiv - Cell Biology 2022Quote: ... 0.05% uranyl acetate in acetone and placed in an AFS-2 Freeze-Substitution Machine (Leica Microsystems, Vienna). Samples were freeze-substituted at -90°C for 72 h ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5% glutaraldehyde and 0.25% uranyl acetate for 60 h in an FS system (AFS-2; Leica Microsystems). The embryos were embedded in an Epon-Araldite mix (EMS hard formula) ...
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bioRxiv - Immunology 2022Quote: ... Paraffin embedded kidney sections (2 µm) were cut on a Leica RM 2165 microtome (Leica, Wetzlar, Germany) and dried at 60°C for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... images were captured every 2 h for 72 h by the IncuCyte Live-Cell analysis system (Leica).
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bioRxiv - Developmental Biology 2023Quote: ... 0.5% glutaraldehyde and 0.25% uranyl acetate for 60 h in a FS system (AFS-2, Leica Microsystems). Larvae were embedded in Epon-Araldite mix (EMS hard formula) ...
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... 2 μm thin slides were then prepared and immunohistochemistry was performed on a Bond Rxm system (Leica) with primary antibodies against Hexokinase 1 (1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... Semi thin (2 μm) and ultra-thin (70 nm) sections were cut with an ultra-microtome (Leica Ultracut ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen was unmasked with Tris-EDTA pH 9 (Bond Epitope Retrieval Solution 2, Leica Biosystems, cat# AR9640).
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bioRxiv - Biophysics 2020Quote: ... the hind wings are dehydrated and cut into six pieces at the nanoindentation test points at the C+ScA after being embedded in paraffin using a paraffin embedding machine (Leica EG1150C, Leica Biosystems, Germany) to support the specimens in the slicing test ...
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bioRxiv - Developmental Biology 2021Quote: High-pressure frozen samples were vacuumed to 5 × 10−7 mbar at -120 °C and a fracture was conducted at the interface between the two HPF discs containing the sample (BAF 60, Leica Micro-systems, Vienna, Austria). One disc containing the exposed cryo-fixed ...
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bioRxiv - Neuroscience 2020Quote: ... Sagittal sections (25 μm thick) were cut using a Leica CM1900 cryostat at a chamber temperature of −20 °C (Leica Biosystems, Buffalo Grove, IL) and mounted onto Superfrost-Plus Gold microscope slides (Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Plants were watered and left at ambient conditions (24 °C) with lid on top and imaged two days past transformation with an SP8 confocal laser scanning microscope (CLSM) (Leica Microsystems GmbH, Wetzlar, Germany) with LAS AF and SymPhoTime software (Picoquant GmbH ...
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bioRxiv - Cell Biology 2020Quote: ... The fresh frozen brain samples were sectioned sagittally at 14 μm thickness and the frozen brain homogenates at 20 μm thickness with a Leica CM3050 cryostat at −20°C (Leica Microsystems GmbH, Wentzler, Germany). All brain specimens were cut at approximately the same Bregma in order to clearly delineate the hippocampus ...
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bioRxiv - Biophysics 2022Quote: ... Confocal images and super-resolution images (Figure 3A, C, D; Figure S2A) were acquired under 100× objectives on a STED confocal microscope (Leica TCS SP8 STED 3X). Images (Figure S2C ...
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bioRxiv - Microbiology 2021Quote: ... pre-warmed at 37°C and the channels were imaged at x64 magnification using a DM-IRE2 confocal laser scanning microscope (Leica Microsystems Heidelberg GmbH, Germany) and the accompanying Leica Microsystems Confocal Software (version 2.61 Build 1537) ...
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bioRxiv - Biophysics 2022Quote: ... nobilis prisms from the prismatic layer of the pinna nobilis shell were fixed along the c-axis of calcite with DI water under -20°C then cut into slices with a thickness of around 30 µm using a Leica Microtome (Leica CM3050 S, Leica Microsystems Nussloch GmbH, Germany). To separate the organic shields ...
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Decoupling cell size homeostasis in diatoms from the geometrical constraints of the silica cell-wallbioRxiv - Microbiology 2023Quote: ... Vitrified samples were kept in liquid nitrogen until freeze-substitution in a freeze-substitution device at −90 °C (EM ASF2, Leica Microsystems GmbH, Wetzlar, Germany). For 48 hours ...
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Decoupling cell size homeostasis in diatoms from the geometrical constraints of the silica cell-wallbioRxiv - Microbiology 2023Quote: ... the discs were loaded into a Leica ICE high pressure freezing machine and vitrified at liquid nitrogen temperature (−192 °C) at 210 MPa (Leica Microsystems GmbH, Wetzlar, Germany). Vitrified samples were kept in liquid nitrogen until freeze-substitution in a freeze-substitution device at −90 °C (EM ASF2 ...
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Decoupling cell size homeostasis in diatoms from the geometrical constraints of the silica cell-wallbioRxiv - Microbiology 2023Quote: ... Epon-embedded samples were cured at 70 °C for 72 and then sectioned to 70 nm thin sections using Ultracut UCT ultra-microtome (Leica Microsystems GmbH, Wetzlar, Germany) equipped with an Ultra 45° diamond knife (Diatome Ltd ...
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bioRxiv - Biochemistry 2023Quote: ... Rat hippocampus tissue punches and thin sections were prepared according to published protocols.33 Rat brain hemisphere slices 16 µm thick were cut at -16°C using a cryostat (Leica CM3050 S, Leica Biosystems GmbH, Wetzlar, Germany). Brain slices containing hippocampal areas of all three animals were deposited on the same slide ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was incubated for 30 s at 90 % relative humidity and 16°C inside Leica EM ACE 600 (Leica EM GP2 plunge freezer), blotted for 1.2 s and vitrified in liquid ethane at liquid nitrogen temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Tissues were cast into molds together with liquid paraffin and cooled to form a solid paraffin block with embedded tissue (Leica EG1150 H Paraffin Embedding Station and Leica EG1150 C Cold Plate). Paraffin-embedded WAT was sliced into 5-μm sections using a ThermoScientific HM 355S Automatic Microtome and mounted on Surgipath X-tra Microscope Slides (Leica Biosystems).
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bioRxiv - Cell Biology 2023Quote: ... for 4 hours and followed by Lowicryl HM20 for 24 hours with agitation and polymerized under UV exposure at −45 °C in an automated processor (AFS2, Leica Microsystem Inc., Vienna, Austria). Ultrathin sections (90 nm thickness ...
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bioRxiv - Developmental Biology 2021Quote: ... 1/1000 (Leica biosystems), Rabbit anti-PH3 ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were embedded in 4% low temperature gelling agarose and cut into 100-150 micrometer thick section using a vibratome (Leica VT1200 S). The Proximity Ligation Assay (PLA ...
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bioRxiv - Cancer Biology 2021Quote: Larvae fixed in 4% PFA overnight at 4°C were embedded in 4% low melting temperature agarose and transverse sections collected at 200 µm intervals using a vibrating microtome (Leica VT 1000S). Sections were blocked with 1% BSA in PBS/0.3% Triton X-100 and incubated with a 1:750 dilution of mAb414 (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... brains were post-fixed for 24 hours and then included in agar 4% and serial cuts of 40 µm thick were made using vibratome (Leica Biosystems, S2000).
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bioRxiv - Cancer Biology 2021Quote: ... The specimens were embedded in paraffin blocks and serial sections of 4 μm thickness were cut using a microtome (Leica Biosystems, Germany). The sections were stained by Hematoxylin & Eosin (H&E)34 Histological photographs were obtained using a digital light microscope (Olympus ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were postfixed in 4 % PFA for 24 hours until they were sliced (70 µm thickness) using a Leica Vibratome (Leica, Wetzlar, Germany). Slices were directly mounted on an object slide (Gerhard Menzel ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were post-fixed overnight in 4% paraformaldehyde and then cryoprotected in a 30% sucrose and 0.1M PBS solution prior to sectioning (Leica CM3050S, Leica Biosystems). Three sets of immediately adjacent sections (40 μm ...
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bioRxiv - Molecular Biology 2022Quote: ... Green siliques were harvested 12 d after flowering from 4-6 week old plants and opened using fine needles under a dissecting microscope (Leica MZ9.5, Germany). Images of opened siliques were captured using a Leica DFC290 (Germany ...
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bioRxiv - Microbiology 2022Quote: ... Grids were sputter-coated with a ∼4 nm thick layer of tungsten using ACE600 cryo-sputter coater (Leica Microsystems GmbH, Vienna, Austria) and afterwards transferred into a Crossbeam 550 FIB-SEM dual-beam instrument (Carl Zeiss Microscopy ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were post-fixed overnight in 4% PFA and stored in PBS until histological slicing was performed using a vibratome (Leica VT1200 S) and the slices were mounted in DAPI-Fluoromount-G (70-100 µm slices ...
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bioRxiv - Plant Biology 2019Quote: ... A series of 4-μm thick longitudinal sections and transverse sections (rapidly elongating region) were made with a rotary microtome RM2265 (LEICA EM UC7). Fixed sections were stained with toluidine blue O for 30 min and photographed with an Olympus BX51 microscope equipped with a DP74 camera.
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bioRxiv - Cancer Biology 2021Quote: ... Formalin-fixed paraffin-embedded (FFPE) samples were cut to a thickness of 4 μm and stained on an automated stainer (BOND-MAX, Leica Biosystems, Germany) as part of the diagnostic routine in a clinically accredited histology lab ...
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bioRxiv - Neuroscience 2023Quote: ... Ribbons of 150-200 serial sections of thicknesses of approximately 55nm were cut with 4 drops of pure ethanol in the water boat and an ionizing instrument (Leica EM Crion) activated and oriented towards the cutting edge of the knife from above ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were dehydrated using an ascending ethanol series (30%-100%) at 4℃ and then critically dried using a low-speed critical point dryer (Leica EM CPD300). Subsequently ...
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bioRxiv - Developmental Biology 2023Quote: ... The oocytes were fixed in 4% PFA/PBS for 30 min and observed using the Leica M205FA stereomicroscope (Leica Microsystems, Wetzlar, Germany) or FV3000 Confocal Microscope.
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bioRxiv - Neuroscience 2023Quote: ... Images of cultured ependymocytes co-treated with Sir-Actin and calcein AM were captured at 10-s intervals for 12 minutes while that of co-treated with Sir-Actin and fluo-4 AM were captured at 10-s intervals for 6 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The intensity of F-actin area from all Sir-Actin positive cells in a field of recording were included in the statistics ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescence signals of Fluo-4 were monitored at 10-s intervals for 6 minutes by confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). As previously described 46 ...
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bioRxiv - Bioengineering 2024Quote: ... and fetal pig kidneys were fixed with 4% paraformaldehyde (FUJIFILM Wako Pure Chemical Corporation) and sectioned with a cryostat (Leica Microsystems GmbH) to prepare frozen sections ...
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bioRxiv - Bioengineering 2024Quote: ... The fixed constructs were embedded in low-melting point agarose 4% w/v in pre-warmed PBS and sliced to a thickness of 300 μm with a vibratome (Leica Biosystems, VT1000S) filled with PBS 1x ...
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bioRxiv - Molecular Biology 2021Quote: ... embedded in OCT tissue freezing medium and 5 μm cross-sectioned on a Cryostat (Leica CM3050 S, Leica Biosystems, USA). Cross-sections were placed in 1x TBS for 10 minutes ...