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Citations for Leica :
1651 - 1700 of 2407 citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The lumbar region of spinal cord was embedded in low-melting point agarose (4% solution, in PBS) and sectioned into 40 µm thick coronal sections with a vibrating microtome (Leica VT1000S). For KCC2 staining ...
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bioRxiv - Neuroscience 2022Quote: ... The brain was dissected out followed by post-fixation in 4% paraformaldehyde overnight and sectioned at 50μm using a Leica vibratome (Leica VT1000S, Leica Inc., Germany). Serial floating sections (300μm apart ...
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bioRxiv - Neuroscience 2022Quote: ... mice were perfused with 4% PFA in PBS and brains were removed and sectioned coronally or sagittally on vibratome (Leica VT1000) at 50-75 μm and mounted on slides with ProLong(tm ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde in PBS and imaged using laser scanning confocal microscopy (LEICA DMi8, Leica Microsystems, Wetzlar, Germany).
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bioRxiv - Genetics 2022Quote: ... The left femur was decalcified after μCT scanning and were embedded in paraffin and cut into 4-μm sections using a microtome (Leica RM2016, Leica Microsystems). Hematoxylin & eosin (H&E ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed with 4% PFA in PB overnight and 50-µm coronal sections of the brainstem were obtained on a vibratome (Leica VT1200S). Expression of EGFP at the calyx of Held terminals was visualized using an epifluorescence inverted microscope (CKX41 ...
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bioRxiv - Pathology 2023Quote: ... the specimens were paraffin-embedded and subsequently sectioned into 4-μm-thick tissue slices by a microtome (RM2235, Leica Microsystems Inc.). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... Paraffin blocks were then sectioned at 4 μm thickness and processed for H&E staining and the 20X objective of the Aperio-XT slide scanner (Leica Biosystems) was used to scan slides ...
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bioRxiv - Immunology 2023Quote: ... from Apoe-/-LysMCre and Apoe-/- LysMCreCnr1flox/flox male mice collected after 4 weeks WD were mounted on RNase-free membrane metal frame slides (POL FrameSlide, Leica Microsystems). Following deparaffinization under RNase-free conditions ...
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bioRxiv - Neuroscience 2023Quote: ... After the brain samples were fixed in 4% paraformaldehyde overnight, they were dehydrated in 30% sucrose (Daejung, Korea) and embedded in frozen section compound (Leica, Germany). Coronal sections (10 µm ...
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bioRxiv - Cell Biology 2023Quote: ... confocal microscope in 3 Z-stacks at 20x (1024x1024 resolution) and further imaged at 63x with immersion oil in 4 Z-stacks to highlight regions of interest (Leica Sp8x).
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bioRxiv - Neuroscience 2023Quote: ... Half brains were fixed in 4% paraformaldehyde for 24h and sectioned at 40 μm thickness in the coronal plane on a vibratome (Leica VT1000S).
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bioRxiv - Microbiology 2024Quote: ... Grids were sputter-coated with a ∼4 nm thick layer of tungsten using an ACE600 cryo-sputter coater (Leica Microsystems GmbH) and afterwards transferred into a Crossbeam 550 FIB-SEM dual-beam instrument (Carl Zeiss Microscopy) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The other hemibrain was drop-fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) and sectioned (30 μm) with a sliding microtome (Leica SM2000R). All mouse experiments were performed in accordance with European Union Council Directive 86/609/EEC ...
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bioRxiv - Neuroscience 2021Quote: ... Spheres were allowed to grow for 5 weeks and were photographed using an inverted bright-field microscope (Leica DMIRBE). Viable spheres were recovered by centrifugation at 100g for 5min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 5-day old Arabidopsis seedling roots expressing AtC53-mCherry were cut and high-pressure frozen (EM PACT2, Leica, Germany), prior to subsequent freeze substitution in acetone containing 0.4% uranyl acetate at −85 °C in an AFS freeze-substitution unit (Leica ...
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bioRxiv - Developmental Biology 2021Quote: Embryos of targeted injections of DshDEP+ into the neural fold were imaged using the Stellaris 5 confocal system with the HC APO L 10×/0.30 NA water immersion objective (Leica). The sections of these embryos were imaged using the LSM 980 confocal system with the LD LCI Plan-Apochromat 25×/0.80 NA oil immersion objective (Zeiss).
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Presynaptic mitochondria volume and abundance increase during development of a high-fidelity synapsebioRxiv - Neuroscience 2019Quote: ... Wafers were coated with a 5 nm layer of carbon before imaging to limit charging (Leica, Wetzlar, Germany, ACE600). Image acquisition was performed semi-automatically using a Merlin VP Compact SEM equipped with Atlas 5 AT software (Carl Zeiss Microscopy LLC. ...
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bioRxiv - Pathology 2020Quote: Frozen tissue blocks were sectioned at 5 µm thickness in a Leica CM1850 cryostat (Leica Microsystems GmbH, Wetzlar, Germany) at -20C ...
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bioRxiv - Developmental Biology 2021Quote: Live imaging of Rx3::H2B-GFP-derived organoids (Video 5 and 6) was performed with Sp8 confocal microscope (Leica). Organoids were imaged from day 1 to day 2 at room temperature directly in low binding 96-well plate (Nunclon Sphera U-Shaped Bottom Microplate ...
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bioRxiv - Zoology 2021Quote: ... Tissue sections with 5 μm thickness were cut using a microtome (Leica RM2235, Leica Biosystems Inc., Buffalo Grove, USA), fixed on slides ...
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bioRxiv - Cancer Biology 2022Quote: ... Invading cells were imaged with a sCMOS Orca-Flash 4.0LT camera (Hamamatsu) coupled 5 to an inverted DMi8 microscope (Leica), capturing serial z-stacks every 10 µm with a 10× objective (dry ACS APO 10x/NA 0.3) ...
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bioRxiv - Neuroscience 2021Quote: ... bubbled with 95% O2-5% CO2 and sliced using a Vibratome (Leica VT1200, Leica Microsystems Inc, Buffalo Grove IL) except for experiments in which high Ca2+ concentrations were used ...
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bioRxiv - Neuroscience 2020Quote: ... Sections of 100µm were performed at medium speed (around 5) and amplitude (approx. 6) using a vibratome Leica VT1000S (Leica). Sections were placed on a glass slide for immunostaining.
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bioRxiv - Neuroscience 2021Quote: ... The paraffin-embedded brains were sectioned in the coronal plane at 5 µm using a microtome (Leica RM 2265). Sections were collected at 40 μm intervals ...
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bioRxiv - Cell Biology 2022Quote: ... Fluorescence images of stained myotubes were obtained in 5 different areas using a DMI 3000 B microscope (Leica, Germany) and analyzed using ImageJ 1.52 software (National institutes of Health ...
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bioRxiv - Cell Biology 2019Quote: ... right after the processing and then sectioned at 5 µm by using Leica RM2255 (Leica Biosystems, Buffalo Grove, IL) for histological staining.
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bioRxiv - Physiology 2021Quote: ... Undecalcified 5 μm and 10 μm thick longitudinal sections were obtained using a microtome (RM2255, Leica Biosystems., IL, USA). 5 μm sections were stained with Goldner Trichome and at least two nonconsecutive sections per sample were examined for measurement of cellular parameters ...
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bioRxiv - Immunology 2021Quote: ... Organs included in paraffin were cut to a thickness of 5 microns (Microtome Leica RM2245 of disposable metal blades) and placed in a flotation solution in a water bath and then fixed on a slide sheet ...
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bioRxiv - Plant Biology 2020Quote: ... The embedded blocks were transversely sectioned at 5 to μm thickness using a Leica RM2125RT rotary microtome (Leica Microsystems) on RNase-free polyethylene naphthalate PEN membrane slides (Leica) ...
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bioRxiv - Neuroscience 2020Quote: ... At least 5-10 flies per genotype were scanned using the Confocal Laser Scanning Platform Leica SP8 (Leica, Germany). Samples were excited with an argon laser at 488nm (20% tube current) ...
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bioRxiv - Physiology 2022Quote: ... The samples were imbedded in paraffin followed by serial sectioning at 5 µM thickness using the Leica microtome (Leica). The sections were deparaffinized ...
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bioRxiv - Biochemistry 2023Quote: ... The paraffin-embedded tissues were sectioned (5 µm thick) with a semi-automated rotary microtome (Leica Microsystems, Wetzlar, Germany) and placed on microscope glass slides coated with poly-L-lysine ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anaesthetized using 2.5% isoflurane and 1.5 l/min air for 5 min and impacted directly over their intact head using an electromagnetic impactor (Leica Impact One Stereotaxic impactor ...
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bioRxiv - Bioengineering 2023Quote: ... the grips were attached to a custom mechanical testing rig mounted on an upright confocal microscope (Leica STELLARIS 5) in a bath with 1× PBS (Fig ...
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bioRxiv - Bioengineering 2022Quote: ... and placed on a microscope slide to image with a confocal microscope (Leica Stellaris 5 confocal with DMI 8). The settings for fluorescent imaging were calibrated using a positive control ...
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bioRxiv - Bioengineering 2023Quote: ... Images (n = 5) of S-shape embedded filaments were taken with a confocal microscope (SPX8, Leica Microsystems, The Netherlands) and analyzed with ImageJ ...
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bioRxiv - Developmental Biology 2024Quote: Fixed samples were imaged in a Leica Stellaris 5 upright confocal microscope using a HC PL APO 40x/1,1 Water CORR CS2 objective (#506425, Leica). The microscope was operated by the LAS X (v4.5.0.25531 ...
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bioRxiv - Immunology 2024Quote: ... The stage-top chamber was set onto the stage of a Stellaris 5 confocal microscope (Leica Microsystems, Deerfield, IL). Transmitted (brightfield ...
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bioRxiv - Neuroscience 2024Quote: ... PL Apo 40x/0.85 CORR CS and PL APO 63x/1.40 oil CS2 and Leica Stellaris 5 confocal microscopes (Leica Microsystems) equipped with a HCPL FLUOTAR 10x/0.30 ...
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bioRxiv - Biophysics 2023Quote: ... Stellaris 5 up-right system using either a HC APO L U-V-I 10x/0.30 NA WATER (Leica) or a HC FLUOTAR L VISIR 25X/0.95 NA WATER (Leica ...
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bioRxiv - Neuroscience 2024Quote: ... saturated with 95 % O2 and 5 % CO2 to obtain 250 μm-thick coronal sections with a vibratome (Leica VT1200S). Slices were transferred and incubated in a holding chamber containing (ACSF ...
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bioRxiv - Biophysics 2023Quote: ... They were imaged with sequential z-stacks using a similar procedure as for the 3D colonies but with an 5× air objective (Nplan5×0.12PHO, Leica). The voxel size in (x,y,z ...
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bioRxiv - Cell Biology 2020Quote: Sample blocks were obtained by freeze substitution of the cell pellets in 0.5 % uranyl acetate diluted from a 20 % stock in methanol and 3 % double distilled H2O in acetone in a Leica AFS1 (Leica Microsystems GmbH, Wetzlar, Germany). Temperature was set to −130 °C for the first 2 hours and then raised to −90 °C with a 20 °C/hr gradient ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Samples were imaged in brightfield using a Point Grey Flea®3 color camera (Teledyne FLIR, Wilsonville, OR) mounted on a Leica S8 APO stereo microscope (Leica Camera, Wetzlar, Germany). Representative images are shown.
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bioRxiv - Pathology 2021Quote: Four micron thick sections of left and right ventricular FFPE tissue blocks per decedent were cut and labeled using an automated staining platform (Leica Bond 3, Leica Biosystems, Buffalo Grove, IL) with the following monoclonal antibodies against ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were rinsed 3 times in phosphate buffered saline and 50 μM coronal brain slices were obtained using a vibratome (Leica VT1000, Buffalo Grove, Il), without embedding the tissue ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were washed an additional 3 times in 100% ethanol for 15 minutes each and dried using a Leica EM CPD030 critical point dryer (Leica Microsystems, Buffalo Grove, IL) with CO2 as the transitional medium ...
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bioRxiv - Bioengineering 2023Quote: ... were acquired over a ∼30 mm x ∼3 mm rectangular area by using the tile scan feature of the LAS X software (Leica Microsystems, Buffalo Grove, IL). This involved frame by frame acquisition (36 × 5 = 180 frames ...
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bioRxiv - Cell Biology 2020Quote: ... The frozen samples were subsequently transferred into a Leica EM AFS-2 freeze substitution unit (Leica Microsystems). Over a period of five days ...