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Citations for Leica :
1401 - 1450 of 2407 citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... prior to subsequent freeze substitution in acetone containing 0.4% uranyl acetate at −85 °C in an AFS freeze-substitution unit (Leica, Wetzlar, Germany). After gradient infiltration with increasing concentration of HM20 ...
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bioRxiv - Immunology 2022Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems). The SARS-CoV-2 probe (catalog number 848568 ...
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bioRxiv - Cell Biology 2022Quote: ... Live-cell imaging was performed at 37°C with a Leica TCS SP8 confocal microscope with a 100×/1.40 Oil HC PL APO CS2 objective (Leica, Wetzlar, Germany) equipped with a white light laser ...
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bioRxiv - Cell Biology 2019Quote: ... The blotting was carried out on the opposite side from the liquid drop and plunge frozen in liquid ethane at −181°C using an automated freeze plunging apparatus (EMGP, Leica, Germany). The samples were kept in liquid nitrogen and imaged using three different microscopes ...
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bioRxiv - Neuroscience 2019Quote: ... brains were flash frozen using crushed dry ice and coronal sections (40 μm) containing the BNST were collected using a cryostat set to −20° C (Leica Microsystems). Sections were wet-mounted onto gelatin-subbed glass microscope slides ...
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bioRxiv - Microbiology 2019Quote: ... After 24h incubation at 37°C images of the halo zones were taken using a Leica S8APO stereomicroscope (Leica, Wetzlar, Germany). The area of halo zones was determined by image analysis in Inkscape (version 0.91 ...
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bioRxiv - Immunology 2021Quote: ... Samples were blotted for 3-4 s at 80% humidity and 20°C and subsequently plunge-frozen in liquid ethane using an EM GP blotter (Leica Microsystems). Grids containing NLRP3PYD filaments were imaged using a Krios Titan TEM (ThermoFisher) ...
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bioRxiv - Neuroscience 2020Quote: ... Motoneurons were patched at room temperature (approx 21°C) using infrared-differential interference contrast (IR-DIC) optics on a DMLFSA microscope (Leica DMLFSA; Leica Microsystems). All lumbar motoneurons were selected from the lateral motor nuclei of segments L1-6 and all cervical cells were selected from the lateral motor nuclei of slices made from C4-8 ...
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bioRxiv - Neuroscience 2021Quote: ... Confocal and STED images were acquired at 23°C with a modified TCS SP5 STED-CW gated (Leica Microsystems, Mannheim, Germany) operated with Leica’s microscope imaging software ...
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bioRxiv - Biophysics 2020Quote: ... the grids were lifted and held by tweezers inside the plunge freezer chamber at 90% relative humidity and 22 °C (Leica EM-GP plunger, Leica Microsystems). 1 μL drop of X5 concentrated gold nanoparticle (150 nm ...
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bioRxiv - Neuroscience 2020Quote: ... The fly head-embedded gelatin was subsequently cut with 12 μm thickness using a cryo-microtome at −20°C (Leica CM1520). Sections were thaw-mounted on indium tin oxide coated glass and freeze-dried overnight in the ToF-SIMS instrument for further analysis.
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bioRxiv - Plant Biology 2021Quote: ... The abaxial side of cotyledons were imaged using a 63x/1.2 water immersion objective lens (Leica C-APOCHROMAT 63×/1.2 water). GFP was excited at 488 nm and fluorescence emission collected between 500-540nm ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were embedded at 60°C for 48 h and cut into 70 nm sections with a microtome (EM UC7, Leica, Germany). After electron staining with uranyl acetate and lead citrate ...
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bioRxiv - Cell Biology 2022Quote: SMLM images were acquired at room temperature (24 °C) on a customized microscope (Mund et al., 2018) with a 160x NA 1.43 oil-immersion objective (Leica, Wetzlar, Germany). Illumination was done using a a LightHub laser combiner (Omicron-Laserage Laserprodukte ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were then frozen in −20°C isopentane and cut into 20 μm-thick coronal sections on a cryostat (Leica CM1900), collected on silane-coated slides and stored at −20°C ...
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bioRxiv - Systems Biology 2022Quote: FLIM of EGFR-mCitrine/PTB-mCherry or PTP-mCitrine/DyTo was performed at 37°C on the Leica SP8 laser-scanning microscope equipped with a fast lifetime contrast module (FALCON, Leica Microsystems) using the 63×/1.4NA oil objective ...
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bioRxiv - Genomics 2022Quote: ... tissue slides were baked overnight at 60°C to ensure tissue adherence to the positively charged glass slides (Leica Bond Plus Microscope slides, Leica Biosystems). Then the tissue was deparaffinized and digested with Protease Plus (ACD Bio ...
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bioRxiv - Plant Biology 2019Quote: ... T1 plants were grown under long day conditions at 22 °C and phenotyped using the z-stack function of a Leica M205 FA microscope (Leica, www.leica.de).
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bioRxiv - Biochemistry 2020Quote: ... Grids were blotted in a climate chamber set to 20 °C and at 95% humidity before plunge frozen in liquid ethane set at −183 °C using a Leica EM GP system (Leica Microsystems). Images containing complexes were collected manually with a 120 kV TALOS transmission electron microscope (Thermo Fisher Scientific ...
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Computational toolbox for ultrastructural quantitative analysis of filament networks in cryo-ET databioRxiv - Cell Biology 2021Quote: ... and vitrification using a Leica GP2 plunger equipped with a blotting detection sensor and incubation chamber maintaining an environment of 21°C and 90% humidity (Leica Microsystems). Grids were placed into the GP2 incubation chamber and excessive liquid was manually removed with a piece of filter paper by gently touching the side of the grid ...
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bioRxiv - Biochemistry 2022Quote: ... The blotting was carried out on the opposite side from the liquid drop and plunge-frozen in liquid ethane at −181 °C using an automated freeze plunging apparatus (EMGP, Leica, Germany). The samples were kept in liquid nitrogen and imaged using a Tecnai G2 (FEI ...
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bioRxiv - Biophysics 2022Quote: ... The blotting was carried out on the opposite side from the liquid drop and plunge-frozen in liquid ethane at –181 °C using an automated freeze plunging apparatus (EMGP, Leica, Germany). The samples were kept in liquid nitrogen and imaged using a Tecnai G2 (FEI ...
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bioRxiv - Biochemistry 2022Quote: ... polymerized at 70°C for 24 hours and then cut to 70 nm ultra-thin sections with an Ultracut S ultramicrotome (Leica, Germany). After cutting ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 s followed by high-pressure freezing after 25 s at -180°C under 2100 bar pressure in an HPM100 machine (Leica Microsystems). After freezing ...
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bioRxiv - Molecular Biology 2022Quote: ... the grid was back-blotted and immediately plunge frozen in liquid ethane at −182°C (Leica Automatic Plunge Freezer EM GP2).
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bioRxiv - Neuroscience 2022Quote: C57Bl/6J wild-type mice were anesthetized with isoflurane and brains were rapidly dissected and frozen on dry ice in OCT embedding medium and stored at -80°C until being sectioned on a cryostat (Leica CM3050s). Ten-micron sections were collected at -20°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Mouse brain was embedded and frozen in optimal cut temperature (OCT) (SAKURA, USA) compound at - 80°C and 10 μm cryosection slices were prepared by using a Leica cryostat (Leica, Germany). The slices were warmed up to room temperature and fixed with 4% PFA for 5 min before used ...
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bioRxiv - Immunology 2022Quote: ... the tissue slides were heat pre-treated using a ER1 (pH6) target retrieval buffer for 15 minutes at 97°C (AR9961, Leica Biosystems). The slides were then stained for multiplex immunofluorescence using the BOND RX Opal multiplex procedure (v7.0.3.216 RX ...
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bioRxiv - Microbiology 2022Quote: ... Cryo-sections (70 nm thick) of the frozen material were obtained at −120°C using an Ultracut cryo-ultramicrotome (Leica Microsystems). The cryo-sections were collected on formvar-coated nickel grids ...
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bioRxiv - Plant Biology 2023Quote: ... Paraplast blocks were then prepared with half seed in each block using the Leica Heated Paraffin Embedding Module EG1150 H with the added Leica Cold Plate for Modular Tissue Embedding System EG1150 C (Leica Biosystems). with vacuum infiltration ...
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bioRxiv - Plant Biology 2023Quote: ... Paraplast blocks were then prepared with dozens of seeds in each block using the Leica Heated Paraffin Embedding Module EG1150 H with the added Leica Cold Plate for Modular Tissue Embedding System EG1150 C (Leica Biosystems) with vacuum infiltration ...
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bioRxiv - Neuroscience 2023Quote: ... thin coronal midbrain cryosections (12 µm) were cut at -20°C and transferred to RNaseZAP-treated PPS FrameSlides (Leica Microsystems, # 11600294). Six sections per FrameSlide ...
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bioRxiv - Developmental Biology 2023Quote: ... and embedded tissue was frozen rapidly and either stored at − 80 °C or immediately sectioned into 20-μm thick frozen sections using a cryostat (Leica CM3050S). Histological sections were placed in Superfrost® slides and placed at -20°C ...
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bioRxiv - Pathology 2023Quote: Carriers containing high pressure frozen samples for cryoFIB-SEM liftout were transferred to a cryo-ultramicrotome (Leica EM FC7, −160°C) equipped with a Trim 45 T1865 diamond knife ...
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bioRxiv - Bioengineering 2023Quote: ... the tissue was placed in a cryostat chamber at -20°C and sliced into 15 µm thick sections (CM1850, Leica microsystems). The FBR to the polymer coatings was assessed by staining with haematoxylin and eosin (H&E ...
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bioRxiv - Cell Biology 2023Quote: The frozen blocks were removed from the-80°C freezer and allowed to sit in the cryostat (Leica CM 1950, #047742456) at-20°C for 1 hour to equilibrate ...
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bioRxiv - Neuroscience 2023Quote: The right hemisphere of each brain was sliced into 300μm coronal sections at −10°C using a cryostat (CM3050 S; Leica, Nuβloch, Germany). Punches of 0.75mm and 1.25mm were used to extract tissue from the frontal cortex ...
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bioRxiv - Molecular Biology 2023Quote: Adult flies containing P[GMR-Gal4] and four copies of P[UAS-arm*] or its variants were frozen at −20°C overnight and photographed with a Leica Stereo Dissecting Scope (Leica DMI6000B) attached to a digital camera ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue blocks were warmed to −20°C for at least 1h prior to sectioning and sectioned at 18 µm using a cryostat (Leica CM3050S) at −20°C ...
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bioRxiv - Bioengineering 2023Quote: ... samples were frozen at -20⍰C and cryosections with 30 μm thickness were prepared Leica CM1950 cryomicrotome (Leica BioSystems, Diegem, Belgium). Slides were then thawed on Superfrost glass slides (Epredia ...
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bioRxiv - Systems Biology 2023Quote: Frozen serial 8 μm sections of mice heart tissues were cut at −20°C with a Microtome (CM1950; Leica, Nussloch, Germany) and mounted on ITO-coated glass slides for MALDI-MSI and on coated glass slides for HE staining ...
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bioRxiv - Neuroscience 2023Quote: ... snap-frozen in powdered dry ice and stored at -80 °C until sectioned at 40 µm in the coronal plane with a Leica SM 2000R freezing microtome (Leica Microsystems). The sections were stored at -20 °C in 24-well tissue culture plates filled with antifreeze solution (30% ethylene glycol ...
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bioRxiv - Immunology 2024Quote: ... tissues were subsequently embedded in OCT and stored at –80 °C before sectioning (6 μm) on a Leica CM1850 Cryostat (Leica Biosystems). Sections were incubated for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... Cultures were inspected with the microscope Leica DM IL Led Fluo and pictures acquired with camera Leica DFC450 C (Leica Microsystem).
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bioRxiv - Neuroscience 2024Quote: ... brains were immediately frozen and stored at −80°C until sectioning at 12 μm on a cryostat (Leica Biosystems, Wetzlar, DE). PVH and ARC tissue was collected from brain sections using laser capture microdissection with a PALM Robot Microbean (Carl-Zeiss ...
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bioRxiv - Cell Biology 2024Quote: ... Microphotographs (N>=6 fish per genotype) were acquired in a Leica DM4000 B microscope coupled to a Leica DFC425 C camera (Leica Microsystems).
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bioRxiv - Neuroscience 2021Quote: ... contusion was induced using a 2 mm convex tip attached to an electromagnetic impactor (Leica). The contusion depth was set to 1.25 mm from dura with a velocity of 4.0 m/s sustained for 300 ms ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 internal Hybrid (HyD) detectors and Acusto Optical Tunable Filter (Leica microsystems CMS GmbH, Germany) was used and excitation was done by using 488nm by Ar laser and emission was collected using the internal HyD detector at 510-625nm with gain of 100.
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bioRxiv - Plant Biology 2021Quote: ... 9.3% water in water-free acetone in a Leica AFS 2 (Leica Microsystems, Wetzlar, Germany) as described previously (Flechsler et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Slides underwent heat-induced epitope retrieval with Epitope Retrieval Solution 2 (pH 9.0, Leica AR9640) at 95°C for 5 mins ...