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Citations for Leica :
1201 - 1250 of 2407 citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... organs were frozen at −80 °C and then sectioned at 20-μm-thick with the freezing microtome (Leica CM1950) for immunofluorescence labeling ...
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bioRxiv - Neuroscience 2022Quote: ... and mounted onto Über plus printer slides (InstrumeC) in a water bath set to 42°C (Leica Biosystems H1210). Sections were left to dry for 72 hours at room temperature prior to immunohistochemical staining.
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bioRxiv - Molecular Biology 2023Quote: ... Each specimen was frozen at - 20 °C using a Leica CM1950 cryostat (Leica CM1950; Leica Biosystems Nussloch GmbH, Germany) and trimmed until the specimen appeared on the cutting surface ...
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bioRxiv - Plant Biology 2022Quote: ... followed by freeze substitution in dry acetone containing 0.4% uranyl acetate at −85°C in a freeze-substitution unit (EM AFS2, Leica). After three acetone rinses at −85°C ...
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bioRxiv - Genomics 2022Quote: ... tissue slides were baked overnight at 60°C to ensure tissue adherence to the positively charged glass slides (Leica Bond Plus Microscope slides ...
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bioRxiv - Neuroscience 2022Quote: ... frozen sapphire sandwiches were incubated acetone containing 0.1% tannic acid at –90 °C for 20 – 24 h inside an AFS machine (Leica) under constant agitation ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorescence lifetime imaging microscopy (FLIM) analysis was performed at 37°C using a confocal laser scanning microscope (SP8, Leica) equipped with a Fast Lifetime Contrast (FALCON ...
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bioRxiv - Neuroscience 2023Quote: ... The brains were then embedded in OCT and preserved at –80°C until cryosectioning at 60 µm (Leica CM3050). For free-floating immunostaining ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Twenty micrometer-thick coronal sections of mPFC were prepared at -15°C with the Leica CM1860 UV (Leica, Germany) and stored at -80°C until further processing ...
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bioRxiv - Neuroscience 2023Quote: ... samples were transferred under liquid nitrogen to an automated freeze substitution system at -90 °C (EM AFS2, Leica Microsystems). Using pre-cooled tweezers ...
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bioRxiv - Plant Biology 2023Quote: ... Grids were blotted for 2.5 s at 10 °C and 95 % humidity using a Leica EM GP plunger (Leica). Data for NCPs was acquired at the Cryo-EM facility of the Gene Centre in Munich ...
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bioRxiv - Microbiology 2023Quote: ... Trypanosomes at 1x106 parasites/mL were maintained in matrix-dependent HMI-11 medium containing 0,5% or 1.1% methylcellulose at 37°C and were observed under the 10x objective of an inverted DMI-4000B microscope (Leica) coupled to an ORCA-03G (Hamamatsu ...
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bioRxiv - Biophysics 2023Quote: ... We cut the frozen samples in 20 µm thick serial sections at -20 C using a cryostat (Leica Biosystems), and mounted them on poly-L-lysine coated slides ...
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bioRxiv - Cell Biology 2023Quote: ... the high-pressure-frozen samples were freeze-substituted in 0.2% glutaraldehyde with 0.2% uranyl acetate in acetone at -90°C in an automated freeze-substitution device (Leica AFS). After 3 days ...
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bioRxiv - Biochemistry 2023Quote: ... the grid was blotted for 1.5 s on the other side under 99% humidity and at 20 °C using EM GP2 plunge freezer (Leica) and immediately plunged into liquid ethane ...
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bioRxiv - Cell Biology 2024Quote: ... equipped with environmental chamber (Life Imaging Services) at 28°C using a HC PL APO 40x/1.10WCS2 objective (Leica). Whole embryo imaging was performed with a M205FCA stereomicroscope (Leica ...
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bioRxiv - Microbiology 2023Quote: ... and inhibitor-induced effects on worm motility assessed using a stereo microscope at 10x magnification (M125 C, Leica, Germany) using the following scores ...
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bioRxiv - Cancer Biology 2023Quote: ... equipped with Mai-Tai and Insight X3 tunable lasers (Spectral Physics) and a 37 °C preheated 40X objective (NA 1.10, HC PL IRAPO, Leica). The specimens were excited at 910 nm.
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bioRxiv - Molecular Biology 2023Quote: ... Inhibitor-induced effects on worm viability were assessed using a stereo microscope at 10× magnification (M125 C, Leica, Germany) and the following scores ...
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bioRxiv - Genetics 2023Quote: ... Sections were floated onto a 45 °C water bath and mounted on Leica “X-tra” adhesive slides (Leica biosytems), drained ...
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bioRxiv - Neuroscience 2024Quote: ?ssembloids were imaged under environmentally controlled conditions (37°C) using a 5x objective in a confocal microscope (Leica SP8). For glutamate uncaging experiments ...
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bioRxiv - Cell Biology 2024Quote: ... MDM cells were subsequently plunge frozen in liquid ethane at -183°C using a EM GP plunger (Leica Microsystems), with a set chamber humidity of 90% and temperature of 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... NKX2-1 (Leica TTF-1-L-CE), Ki67 (Abcam ab16667) ...
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bioRxiv - Plant Biology 2022Quote: ... and basal sections (1:1:1 ratio) using a stereo microscope (Leica MZ16). Sections were immediately placed into 96-well plates (on ice ...
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bioRxiv - Microbiology 2022Quote: ... A pellet volume of 1.4 μl was dispensed on the 200 μm side of a type A 3 mm gold platelet (Leica Microsystems), covered with the flat side of a type B 3 mm aluminum platelet (Leica Microsystems) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The embryos were cleared in xylene and processed for paraffin 7µm sections as described before [3] using microtome (RM2035, Leica, Germany). Following deparaffinization and rehydration ...
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bioRxiv - Cell Biology 2020Quote: ... elastic modulus and dissipation were performed on a Catalyst BioScope atomic force microscope (Bruker, Santa Barbara, CA) combined with a 3-channel confocal microscope TCS SP5 II (Leica; 20×/NA 0.70 or 40×/NA 0.85 air objectives) ...
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bioRxiv - Bioengineering 2020Quote: ... Briefly, mice were anesthetized with isoflurane (3% induction, 1.5% maintenance and secured on a stereotaxic frame (Leica Microsystems, Wetzlar, Germany). A 3 mm craniotomy (−1 AP mm bregma ...
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bioRxiv - Plant Biology 2021Quote: ... Cells cultured in M9 or LS medium containing 3% sucrose were added to flat specimen carriers and frozen using a high-pressure freezing machine (Leica EM PACT ...
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bioRxiv - Cell Biology 2021Quote: ... 7F and S5D are average projections of time-lapse movies acquired a maximum speed during 3 minutes using a spinning-disk microscope composed of an inverted microscope (DMI4000B; Leica) equipped with an HCX Plan Apochromat 100 × /1.46 NA oil objective (PerkinElmer ...
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bioRxiv - Neuroscience 2020Quote: ... whereas slices processed for immunofluorescence were embedded in 3% agarose solution and re-sliced at 40-µm thickness using a vibratome (Leica VT1200S ...
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bioRxiv - Cell Biology 2021Quote: ... Grids were blotted between 2.5 and 3 sec and plunged frozen in liquid ethane using a Leica vitrification plunger (EM GP2, Leica Microsystems). For RiboRad6mix ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were fixed with 3% formaldehyde and permeabilized with 0.1% Triton X-100 and imaged in Leica Fluorescence microscope (Leica Microsystems). Images were analyzed by ImageJ software.
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Targeting Noradrenergic Neurons of the Locus Coeruleus: A Comparison of Model Systems and StrategiesbioRxiv - Neuroscience 2022Quote: ... After fixation brains were embedded in 3% agarose (Genaxxon Bioscience) and coronal slices of ~50 μm thickness were obtained using a vibratome (VT 1000S, Leica). Slices were stored in PBS at 4°C until they were stained and mounted on microscope slides.
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bioRxiv - Neuroscience 2021Quote: ... the pituitaries were embedded in 3% agarose (H2O) and para-sagittally sectioned with 60 μm thickness using a vibratome (Leica). From a single pituitary ...
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bioRxiv - Microbiology 2022Quote: ... the cell pellet was spread on the 200-μm side of a 3-mm type A gold plate (Leica Microsystems) covered with the flat side of a 3-mm type B aluminum plate (Leica Microsystems ...
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bioRxiv - Neuroscience 2019Quote: Mice were anesthetized with isoflurane inhalant gas (3%) in an induction chamber and positioned in a stereotaxic frame (myNeuroLab, Leica Microsystems Inc. ...
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bioRxiv - Physiology 2019Quote: ... IF was performed on free-floating sections obtained after the tissues were included in 3% agarose and parasagittaly sectioned (60 μm) with a vibratom (Leica). Because the fluorescence of the endogenous DsRed2 is quenched with the epitope retrieval treatments required for BrdU and PCNA staining ...
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bioRxiv - Cell Biology 2020Quote: ... embedded in paraffin and sectioned into 3-μm thick slices using a semi-automated rotary microtome (Leica Biosystems, Wetzlar, Germany). The slices were floated in a 37 °C water bath and mounted either on frosted-edge slides for histological staining (Hurst Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Images were acquired by confocal microscopy using a Leica SP5 system controlled by the LAS AF 3 software (Leica Microsystems). For semi-quantitative quantification of the fluorescence intensity ...
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bioRxiv - Neuroscience 2022Quote: ... embedded into 3% low melting point agarose and then sectioned into 50-60 um using a vibratome (Leica, cat# VT1000S), mounted for direct visualization of EGFP or processed for immunofluorescence as it follows ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the lamps were positioned above zebrafish embryos in 6-well plates such that the power of UV irradiation was ∼5 mW/ cm2 [as measured by a hand-held power sensor (Spectroline, XDS-1000] and ∼3 mW/cm2 for cells. For all imaging experiments, a Leica M205-FA microscope equipped with a stereomicroscope was used ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was embedded in 3% agarose (in H2O) and sectioned parasagitally with 70 μm thickness using a vibratome (Leica). The slices were mounted using Vectashield H-1000 Mounting Medium (Vector ...
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bioRxiv - Plant Biology 2022Quote: ... 5-day-old wild-type Arabidopsis roots or 3-week-old true leaves were frozen in a high-pressure freezer (EM ICE, Leica), followed by freeze substitution in dry acetone containing 0.4% uranyl acetate at −85°C in a freeze-substitution unit (EM AFS2 ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3-8 randomly chosen fields per view per Matrigel assay were acquired using confocal Z-stack imaging (TCS-SP8; Leica). Attached pericytes were counted by using the LASX Software.
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bioRxiv - Cell Biology 2023Quote: ... Tissues from at least 3 independent mice were imaged using an SP5X laser scanning confocal microscope (Leica Microsystems CMS GmbH) or an LSM980 microscope and ZEN Desk 3.4 software (Carl Zeiss GmbH) ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue blocks of the substantia nigra were then embedded in 3% agarose and sectioned using a vibratome (Leica, VT1000S, Germany).
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bioRxiv - Bioengineering 2023Quote: ... z-stacks were taken at a minimum of 3 places on the film and post-processed with Thunder image analysis (Leica) to remove background fluorescence.
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bioRxiv - Neuroscience 2023Quote: ... Brains were post-fixed 24h in 4% PFA before being cut coronally and serially (10 series of 40 µm sections or 3 series of 100 µm sections for morphological studies) with a vibratome (Leica).
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bioRxiv - Cell Biology 2023Quote: ... Zeiss) equipped with a 100× /1.4 NA objective lens and a super-resolution confocal microscope (Leica TCS SP8 STED × 3) equipped with a 100× /1.4 NA objective lens were used for cell observation ...