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Citations for Polysciences :
1901 - 1950 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Each culture well was transfected with 1 μg of FLAG-LRRK1 construct and 500 ng of EGFP-Rab7 using polyethylenimine (Polysciences) and cultured at 37°C with 5% CO2 for 36 h before harvesting.
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bioRxiv - Biochemistry 2022Quote: ... dissected midguts were immediately placed in fixative (4% formaldehyde (Polysciences), 0.2% saponin (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... The expression vector was transfected into HEK293T cells using PEI’MAX’ (Polysciences Inc.). Cells were cultured for 40 h post-transfection and detached from the culture vessels using trypsin/EDTA for 5 min ...
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bioRxiv - Immunology 2022Quote: ... with equal amounts of Ig heavy and light chain expression vectors using polyethylenimine Max (PEI- MAX, Polysciences) as a transfection reagent ...
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bioRxiv - Neuroscience 2022Quote: ... was applied to reduce autofluorescence and slides were mounted with Aqua-Poly/Mount medium (Polysciences). Consistent regions of interest were identified based on gyri formation ...
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bioRxiv - Biophysics 2022Quote: ... 100-200 µg of purified bacmid in 1 mL water were incubated with 400 µg of 25000 MW polyethyleneimine (PEI; Polysciences Cat# 23966) in 1 mL water at 55 degC for 30-45 minutes to sterilize ...
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bioRxiv - Cell Biology 2022Quote: Transfection of HEK 293 cells with plasmid DNA was performed with PEI reagent (Polysciences) in a 1:1 (w/w ...
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bioRxiv - Cell Biology 2022Quote: ... The Transfection mix contained 1μl linear polyethylenimine (Polysciences) per 100 ng DNA ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected with a plasmid harboring an import signal of yeast mitochondrial outer membrane Tom70p fused to EGFP21 using pEGFP-N1 as a backbone (Mito-EGFP) using PEI Max (Polysciences) and cultured for 2 days ...
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bioRxiv - Cell Biology 2022Quote: ... were transiently co-transfected with 4xPylT/PylRS(Y306A, Y384F) and 4xPylT/EMC3(Amber[TAG])-GFP plasmids at a ratio of 4:1 using PEI “MAX” (Polysciences, USA). The cells were grown in the presence of 0.5 mM AbK (Iris Biotech ...
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bioRxiv - Cell Biology 2022Quote: Plasmids were transfected into HeLa Kyoto and HEK293 cells using linear polyethylenimine (PEI, MW 25,000; Polysciences). One day prior to transfection ...
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bioRxiv - Cell Biology 2022Quote: ... An EMC3 knockdown suspension cell line was generated by transient transfection of Expi293 cells with an EMC3 sgRNA cloned into lentiCRISPR v2 using PEI “MAX” (Polysciences, USA). Transfected cells were treated with 10 μg/ml puromycin for four consecutive days ...
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bioRxiv - Biophysics 2022Quote: ... 100 μg mL-1 streptomycin and 5% CO2 atmosphere at 370 C were transiently transfected with wild type human and rat NTSR1 in pcDNA3.1 (4 μg DNA per 75% confluent 10 cm dish) via hybridization with polyethylenimine (PEI, Polysciences, Inc.). The following day ...
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bioRxiv - Cancer Biology 2022Quote: ... and 7.5 µg of DNA expression vector) with 30 µg polyethylenimine (PEI, Polysciences, 23966, USA), and concentrated with Lenti-X concentrator (CloneTech ...
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bioRxiv - Cancer Biology 2022Quote: ... transfection of HEK293T cells was performed using poly(ethyleneimine) (PEI) (Polysciences, Inc). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... and mounted with 18.8 μm (cortex imaging) or 20 μm (midplane imaging) polystyrene beads (Polysciences, Inc.) between a slide and coverslip as in (Rodriguez et al. ...
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bioRxiv - Cell Biology 2022Quote: ... embryos were dissected in 8-10 μL of Shelton’s Growth Medium (Edgar and Goldstein, 2012) and mounted with 20 μm polystyrene beads (Polysciences, Inc.) between a slide and coverslip ...
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bioRxiv - Cell Biology 2022Quote: ... adult worms were treated with 0.0025 mM levamisole in polystyrene polybeads (Polysciences) and mounted on 5% agar pads ...
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bioRxiv - Cell Biology 2022Quote: ... pMlink-FAK was transiently transfected into the cell with polyethyleneimines (PEIs) (Polysciences). The transfected cells were cultured at 37°C for 48 hours before harvesting ...
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bioRxiv - Biophysics 2022Quote: ... the excess solvent was blotted and 1% uranyl acetate (Uranyl Acetate 98%, ACS Reagent, Polysciences, Inc.) was added on the grid ...
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bioRxiv - Biophysics 2022Quote: ... Cells were plated to 1.5 x 105 cells per well in 12-well plates 24 hours prior to transfection with polyethylenimine PEI (Polysciences). The primary transfection mixtures contained 125 ng pFLuc-TAR-140 reporter plasmid ...
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bioRxiv - Genomics 2022Quote: ... DNA was mixed with 4.25X polyethylenimine (PEI) (Polysciences #23966) in at total of 2 mL Dulbecco’s Phosphate-Buffered Saline (DPBS per plate) ...
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bioRxiv - Genomics 2022Quote: ... aligned nanofibrous dual-component scaffolds were developed with approximately a 50:50 ratio of PCL and water-soluble polyethylene oxide (PEO; 200 kDa, Polysciences, Inc., Warrington, PA). Briefly ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the cells were treated with glutaraldehyde and osmium tetroxide and then dehydrated through a graded series of ethanol before they were embedded in Spurr’s resin (Polysciences Inc., Warrington, PA). Rapid freezing and the freeze-substitution method were performed as previously described (36) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The specimens were returned to room temperature with a stepwise temperature rise before they were embedded in Spurr’s resin (Polysciences Inc.). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... 5×106 K562 cells were cross-linked with 0.1% formaldehyde (Polysciences, 04018) and slowly rotated for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... lentiCRISPR v2 plasmids [55] were transfected using polyethylenimine (Polysciences) into 293T cells along with psPAX and pMD2.G packaging plasmids (Addgene ...
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bioRxiv - Immunology 2022Quote: Secreted IgG was produced by co-transfecting 106 293F cells/mL with the paired heavy and light chain expression plasmids at a ratio of 1:1 in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Immunology 2022Quote: ... HEK293E cells were transfected at a density of 106 cells/mL using 0.5 mg/mL of pTT3-RBD-HISAVI DNA and 2 mg/mL filter sterilized pH 7.0 Polyethylenimine (PEI) MAX (40,000 MW, Polysciences). Six days later ...
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bioRxiv - Immunology 2022Quote: ... using polyethylenimine (Polysciences). Transfected cells were allowed to grow in 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... pTRIPZ for BirA*-ZNF524 constructs and pInducer20 for ZNF524-HA constructs) using linear polyethylenimine (PEI MW 25,000, Polysciences Inc.) and the medium was exchanged after 24 h ...
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bioRxiv - Biophysics 2021Quote: ... carboxylated 3 µm silica beads (Polysciences) were coated with a mixture of biotinylated pentameric FN7-10 (a four-domain segment of fibronectin responsible for cell binding and containing the RGD and PHSRN motifs70 ...
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bioRxiv - Biophysics 2021Quote: ... coated with polyethlenimine (100 µg/ml; Polysciences). Neurons were incubated in 5% CO2 at 37 °C ...
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bioRxiv - Biophysics 2021Quote: ... and 990 nm (30 nm, Polysciences, Inc.). Brain slices ...
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bioRxiv - Biophysics 2021Quote: ... 372 nm (10 nm, Polysciences, Inc.), 520 nm (16 nm ...
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Novel amiloride derivatives that inhibit bacterial motility across multiple strains and stator typesbioRxiv - Microbiology 2021Quote: ... and incubated for 10 mins and washed with motility buffer and flushed with 1 μm polystyrene bead (Polysciences, Inc) solution ...
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bioRxiv - Cancer Biology 2021Quote: ... tumors were stained using the picrosirius red stain kit (Polysciences Inc., Warrington, PA). For fibronectin staining ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissues were fixed in zinc formalin (Polysciences, 21516) overnight at room temperature and maintained in 70% EtOH before being processed for paraffin embedding.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were mounted with Aqua Poly/Mount (Polysciences) and imaged using a Zeiss LSM510 META or LSM880 laser confocal microscope under a 40x objective ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μL PEI (Polysciences, #23966-1) in 50 μL Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... tissues were dissected in PBS and immediately fixed in 4% paraformaldehyde (PFA, Polysciences Inc) at room temperature for a minimum of 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... All whole digestive tract was removed and fixed in PBS and 4% electron microscopic-grade paraformaldehyde (Polysciences, USA) for 35 min ...
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bioRxiv - Cancer Biology 2021Quote: ... and the envelope protein expressing plasmid (VSV-G) using polyethylenimine (PEI 25K™; Polysciences; Cat. No. 23966) transfection reagent ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with the corresponding plasmids using polyethylenimine (1 μg/μL [pH 7]; Polysciences) in a 1:3 ratio ...
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bioRxiv - Neuroscience 2021Quote: ... washed again three times for 10 min in 0.1 M PBS and coverslipped with Aqua-Poly/Mount (Polysciences).
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bioRxiv - Neuroscience 2021Quote: ... and embedded in PolyBed® 812 resin (Polysciences, Germany). Ultrathin sections (60-80 nm ...
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bioRxiv - Immunology 2021Quote: ... Tissues were washed in PBS and then fixed with 4% PFA (Polysciences). After 20 min ...
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bioRxiv - Immunology 2021Quote: ... Tissues were mounted with Aqua Polymount (Polysciences). Tissues were imaged using an Olympus FluoView 3000 confocal microscope (Olympus ...
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bioRxiv - Immunology 2021Quote: ... The tissues were then washed with 60% isopropanol and mounted in an Aqua Polymount (Polysciences). Tissues were imaged using an Olympus FluoView 3000 confocal microscope (Olympus) ...
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bioRxiv - Immunology 2021Quote: ... Aqua Polymount (Polysciences) was used to mount the sample ...