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Citations for Polysciences :
1701 - 1750 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The transfection solution was prepared by combining 25 µl (per dish hereafter) of polyethylenimine (PEI) Max solution (1 mg ml-1; Polysciences), 1 ml of Opti- MEM (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transiently transfected with GFP-tagged versions of the hnRNPDL-1/-2/-3 isoforms and linear polyethylenimine (PEI; Polysciences, Eppelheim, Germany) in a 1:3 DNA:PEI ratio ...
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bioRxiv - Microbiology 2022Quote: ... Samples were FS-processed and embedded in Lowicryl HM20 resin (Polysciences) according to a modified protocol of Kukulski et al ...
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bioRxiv - Bioengineering 2022Quote: Fluorescent polystyrene microbeads 500 nm in diameter (Polysciences Inc, USA) were embedded in polyacrylamide gels with a stiffness of 11 kPa ...
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bioRxiv - Biophysics 2022Quote: ... Plasmid constructs were transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was fixed with 4% paraformaldehyde (Polysciences, Cat# 18814-20) for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The circulating hemocytes were fixed with 4% paraformaldehyde (Polysciences, Cat# 18814-20) and centrifuged for 5 min at 1000 rpm before incubation for 10 min at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... with the density of about 2.5 million cell/ml were transfected using 3 mg plasmid by poly-ethylenimine (PEI, Polysciences) with a 1:3 plasmid to PEI weight ratio ...
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bioRxiv - Biophysics 2022Quote: ... and pCDH CMV calnexin-Skylan-S—using polyethylenimine (PEI, Polysciences, USA). At 48 h and 72 h post transfection ...
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bioRxiv - Cancer Biology 2022Quote: We transfected lentiviral plasmids together with gag-pol (pCMV Δ R8.91) and V-SVG (pMD.G VSVG) expressing vectors into HEK293T cells using PEI transfection reagent (Polysciences, #24765). We collected viral supernatants after 48 h and used them to infect the Jurkat human cell line by spinoculation with 4 μg ml-1 Polybrene Infection/Transfection Reagent (Fisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... we diluted a microsphere suspension (Polybead Sulfate Microspheres, 1 μm; Polysciences, Cat. No. 19404-15) by 1000-fold in the LB broth to a final concentration of 4.55×107 particles/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with either 1 mg/ml polyethylenimine (PEI; Polysciences, Inc.) or Effectene transfection reagent (Qiagen) ...
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bioRxiv - Cell Biology 2022Quote: All transfections were perfomed by mixing DNA and polyethylenimine (PEI, Polysciences, 23966) in a 1:3 ratio (μg DNA/μg PEI ...
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bioRxiv - Developmental Biology 2022Quote: ... and the AGM vector library were co-transfected using polyethyleneimine (PEI) (Polysciences, Warrington, PA, USA) as previously detailed (Petazzi et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... The free nuclei were then bound to 300 μL of activated concanavalin A beads (Polysciences #86057) at RT for 10mins ...
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bioRxiv - Cell Biology 2022Quote: ... encoding Cas9 and gRNAs for Cldn2 (sequences are shown in Fig. S3) using PEI-max (Polysciences, PA, USA). The next day ...
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bioRxiv - Cell Biology 2022Quote: ... 9 µg transfer vector and 2 µg pVSV-G using 60 µl linear PEI (Polysciences). Medium was exchanged 6-8 hours post-transfection and harvested after another 48 hours ...
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bioRxiv - Cell Biology 2022Quote: ... 300 (24321-15) and 100 nm (24041-10) were purchased from Polysciences. Wortmannin (HY-10197 ...
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bioRxiv - Cell Biology 2022Quote: ... packaging plasmid by using polyethylenimine (Xiang et al. 2012) (#23966-1, Polysciences) reagent as manufacturers’ instruction ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.1% uranyl acetate (Polysciences, USA). Freeze-substitution was done using an automatic freeze substitution machine (EM AFS ...
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bioRxiv - Cancer Biology 2022Quote: ... Transfection of packaging cells was performed using polyethyleneimine (Polysciences, 23966-2) by mixing with DNA in a 3:1 ratio ...
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bioRxiv - Microbiology 2022Quote: ... 6 μm polystyrene microspheres (Polysciences, USA) were used to calibrate the flow cytometer and evaluate cell size ...
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bioRxiv - Cell Biology 2022Quote: Plasmid transfections were performed with polyethylenimine “MAX” (Polysciences, Cat#24765) to cells at 70% confluency ...
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bioRxiv - Cell Biology 2022Quote: T24 cells were fixed in 2% glutaraldehyde and 2% paraformaldehyde at 4°C overnight and then postfixed in 2% osmium tetraoxide for 1.5 h at 4°C followed by dehydration with ethanol and embedding in Spurr resin (Polysciences Inc., Warrington, PA). Ultrathin sections of the samples were prepared using Ultramicrotome (Leica EM UC7 ...
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bioRxiv - Cell Biology 2022Quote: ... Polyethylenimine (PEI, Polysciences) was used to transfect HEK293T cells with plasmids for StrepTactin- and Streptavidin-based protein purification at 3:1 ratio of PEI:plasmid.
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bioRxiv - Cell Biology 2022Quote: In utero live imaging of oocytes was accomplished by mounting young adult worms with single row of embryos to a 5% agarose pad on a 24×40mm glass coverslip with 1.5 µl each of 15°C M9 buffer and 0.1 µm polystyrene microspheres (Polysciences Inc.), and were gently covered with a 18×18mm coverslip ...
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bioRxiv - Cell Biology 2022Quote: ... AAV-pro 293T cells were transfected with gRNA vectors along with AAV packaging vectors AAV2/9 and pAdDeltaF6 using the PEI MAX transfection reagent (Polysciences, 24765). 72 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Polyethylenimine (Polysciences, 23966-2), Opti-MEM™ (GIBCO ...
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bioRxiv - Developmental Biology 2022Quote: ... Dissected and stained ovarioles and egg chambers were mounted on slides with Aqua-PolyMount (Polysciences, Inc.) media and allowed to harden prior to imaging.
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bioRxiv - Developmental Biology 2022Quote: ... Cells were transiently transfected with three plasmids using Polyethylenimine (PEI, Polysciences Inc. 23966-100). These plasmids were variants of pAAV-MCS ...
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bioRxiv - Developmental Biology 2022Quote: ... Ovaries were either kept whole or dissected further into ovarioles and fixed for 10 mins using 16% methanol-free formaldehyde (Polysciences, Inc., Warrington, PA, USA) diluted to a final concentration of 4% in 1X Phosphate Buffered Saline (PBS) ...
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bioRxiv - Developmental Biology 2022Quote: ... left leg muscles were embedded in embedding cryo-mold (Polysciences, #18986-1) with minimum amount of Tissue-Tek O.C.T ...
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bioRxiv - Genomics 2022Quote: ... The size was defined with a linear model by acquiring time of fight (TOF) of standard sized beads (Megabead NIST Traceable Particles (Polysciences): 60 μm (#64200-15) ...
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bioRxiv - Immunology 2022Quote: Spleens and MLN were harvested from OT-II (Thy1.1) mice and T lymphocytes were enriched using nylon wool fiber columns (Polysciences, Inc., Warrington, PA). CD4+ T cells were positively selected with CD4 (L3T4 ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were transfected with the appropriate SARS-CoV-2 S gene expression vector (wild type or variant) in conjunction with p8.91 (46) and pCSFLW (47) using polyethylenimine (PEI, Polysciences, Warrington, USA). HIV (SARS-CoV-2 ...
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bioRxiv - Genomics 2022Quote: ... the samples were contrasted by 2% uranylacetate (Polysciences Inc, Cat No 21447-25) in water for 1 minute ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Transfection of HEK293T cells was carried out using polyethylenimine (Polysciences Inc.). We used a second-generation packaging system to generate the lentiviruses used in this study (gift from Connie Cepko Harvard Medical School) ...
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bioRxiv - Immunology 2022Quote: ... 100 μL of 2% paraformaldehyde solution (Polysciences, #18814-20 ...
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45-μM filter and stored at −80 °C ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were transfected with 5μg of SARS-CoV-2 spike plasmids using PEI-MAX 40,000 (Polysciences) and incubated for 2 days at 37°C ...
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bioRxiv - Immunology 2022Quote: ... and 6 µl of Fluoresbrite Polychromatic Red Microspheres (Polysciences Inc., Warrington, PA) and the mix was top-plated onto 6-cm NGM plates ...
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bioRxiv - Genetics 2022Quote: ... The animals were immobilized by placing on a 10 % agarose pad with polystyrene beads (Polysciences), which were then covered by a cover slip ...
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bioRxiv - Genomics 2022Quote: ... using polyethylenimine (Polysciences Inc.) at a 3:1 PEI:plasmid ratio ...
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bioRxiv - Immunology 2022Quote: ... p8.74 packaging vector, pseudotyping vector coding for Spike glycoproteins (Wuhan-Hu-1; B.1 Lineage, China) and pREV with PEI (Polysciences, Inc., Warrington, PA, USA) (1 mg/mL in PBS ...
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bioRxiv - Genomics 2022Quote: ... and 10µg of EF1ɑ-ligand IRES-EGFP lentiviral transfer plasmid using polyethylenimine (Polysciences Inc.) at a 3:1 PEI:plasmid ratio ...
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bioRxiv - Immunology 2022Quote: ... by pre-mixing C-terminal AVI-tagged ZM197-ZM233V1V2 or C-terminal AVI-tagged CAP256-wk34c80-RnS-3mut-2G with a furin expression plasmid using Polyethylenimine (PEI) 40K (Polysciences, Inc.) or Turbo293 (Speed BioSystems ...
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bioRxiv - Genetics 2022Quote: ... pCS2-3xFlag-SLP-L or pCS2-3xFlag-SLP-S was transfected into HEK293T cells by polyethylenimine (PEI) MAX (Polysciences, 24765). Cells were harvested 24-hour post-transfection and lysed by RIPA cell lysis buffer (Beyotime ...
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bioRxiv - Genetics 2022Quote: ... and transfer plasmids using polyethylenimine (PEI) MAX (Polysciences, 24765). Cells were harvested by cell lifter (Biologix ...
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bioRxiv - Developmental Biology 2022Quote: ... and then sealed using Aqua-PolyMount (Polysciences, Inc, #18606-20). Images were taken on a Zeiss 700 confocal microscope using a 20X objective ...
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bioRxiv - Plant Biology 2022Quote: ... The 2,000 Da branched polyethyleneimine (06089) was purchased from Polysciences (Germany) and the 5,000 Da branched polyethyleneimine (Lupasol G100 ...