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Citations for Polysciences :
1551 - 1600 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Purified DNA was complexed with PEI-Max (Polysciences, 24765-1) and supplied to culture of the Freestyle human embryonic kidney cell-line (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... Beads were incubated for 2h at RT and then washed in PBS and further incubated for 30 minutes in 1 ml 50 mM ethanolamine (Polysciences). The beads were then washed 3 times in PBS and left in PBS on a cold room rotator.
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bioRxiv - Cancer Biology 2022Quote: ... and LS174T cells done with PEI MAX (Polysciences, 24765-1). DLD-1 cells were transfected with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Live animals were mounted on 10% agarose pads and immobilized using 0.1um polystyrene microspheres (Polysciences 00876-15) [45] ...
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bioRxiv - Cancer Biology 2022Quote: ... and polyethyleneimine polymer (Polysciences Inc; 23966-1), which was pre-incubated for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... or HEK293T cells were seeded for 24h and transiently transfected with the indicated vectors using either polyethyleneimine polymer (Polysciences Inc; 23966-1) TransIT-X2 Dynamic Delivery System (Mirus Bio ...
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KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cellsbioRxiv - Cell Biology 2022Quote: ... and PEI Max (Polysciences) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: Plasmid transfection into HEK293T cells was performed using PEI Max (Polysciences) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were transiently transfected with polyethyleneimine (Polysciences) with different StrepII-GFP-CSPP1 constructs ...
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bioRxiv - Cell Biology 2022Quote: Cell lines were generated by transfecting the SETD1A constructs using polyethylenimine (PEI; Polysciences Inc.) as described earlier (Zargar et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Cryosections were mounted for microscopy on glass slides using Aquamount (Polysciences, 18606), and imaged on a Leica TCS SP8 confocal microscope ...
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bioRxiv - Neuroscience 2022Quote: ... to label the nuclei and the samples were mounted with Aqua Poly/Mount (Polysciences). Primaries and their appropriate secondary antibodies are on supplementary table 1.
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bioRxiv - Neuroscience 2022Quote: ... Coverslips were mounted with Aqua-Poly/Mount (Polysciences, Inc.). We used the following primary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... or Fast Blue (Polysciences) at distinct stages ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μg DNA/ml transfection volume and 5 μg of 40 kDa PEI (Polysciences) were first diluted separately in 5% transfection volume in supplemented F17 media ...
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bioRxiv - Biophysics 2022Quote: ... Cell transfection was done with PEI reagent (24765-1, Polysciences) according to instructions provided by the manufacturers ...
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bioRxiv - Microbiology 2022Quote: ... was studied using 1 μm diameter polystyrene beads (Polybead®; Polysciences) as a standard for all the experiments ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were mounted on slides with Aqua-Polymount (Polysciences) using 1.5 coverslips (Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... then fixed overnight at 4°C in 4% methanol-free formaldehyde (Polysciences, # 040181). Wholemount in situ hybridization (WISH ...
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bioRxiv - Developmental Biology 2022Quote: ... Stained embryos were mounted using Aqua Poly/mount (Polysciences, Warrington, PA) on slides and imaged as described below ...
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bioRxiv - Developmental Biology 2022Quote: ... All samples were mounted using Aqua Poly/mount (Polysciences, Warrington, PA) on slides and imaged as described below ...
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bioRxiv - Cell Biology 2022Quote: ... COS-7 and HEK293 cells were transfected with DNA plasmids using PEI (Polysciences, Inc., Warrington, PA). At 24 hours post-transfection ...
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bioRxiv - Microbiology 2022Quote: ... 293F cells were transfected at a density of 106 cells/mL in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences, cat#24765). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... 293T cells were transfected using polyethylenimine (PEI; Linear, MW 25000 (Polysciences Inc., 23966) dissolved in water (pH 7.4) ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... 5 μg of an HIV-1 Gag-Pol expression plasmid (pCRV1/GagPol) and 1 μg of VSV-G expression plasmid in 293T cells in 10-cm dishes using polyethylenimine (PolySciences). Virus-containing supernatant was collected and filtered (0.2μm ...
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bioRxiv - Microbiology 2022Quote: ... The resulting plasmid was transfected with PEImax (24765-1) (Polysciences, Inc.) in EXPI-293F cells (A14527 ...
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bioRxiv - Pathology 2022Quote: ... Slides were placed in phosphomolybdic acid (Polysciences) for two minutes ...
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bioRxiv - Pathology 2022Quote: ... sections were then placed in Picrosirius Red F3BA stain (Polysciences) for 45 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... AAV7A2 and AAV9A2 caps were used in this study) and adenoviral helper plasmid at a 1:1:1 molar ratio using a polyethylenimine (PEI MAX; Polysciences, Warrington, USA, cat.no. 24765-1) as the transfection reagent ...
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bioRxiv - Physiology 2022Quote: ... Slides were first stained in Weigert’s Iron Hematoxylin solution for 10 min at room temperature (Polysciences Inc., Warrington, PA). This was followed by 2 min wash in tap water ...
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bioRxiv - Immunology 2022Quote: ... The light and heavy chain expression plasmids of LH01 or anti-PD-L1 were mixed at 2:1 and co-transfected using liner polyethylenimine (PEI) with a molecular weight of 25 kDa (Polysciences, Warrington, PA, USA). LH02 was produced by transfecting HEK293E cells with Fc-Sushi-IL15-expression plasmid alone ...
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bioRxiv - Immunology 2022Quote: ... 6 µg pMSCV retroviral plasmid and 3.5 µg pCL-Eco packaging vector were preincubated in 500 µl of complete DMEM at room temperature for 15 min before mixing with 500 µl of 80 µg/ml PEI max (Polysciences, 24765) in complete DMEM ...
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bioRxiv - Developmental Biology 2022Quote: ... brains were mounted in Aqua Polymount (Polysciences, Inc).
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bioRxiv - Genetics 2022Quote: Circulating blood monocytes were labeled in vivo by retroorbital intravenous injection of 1 μm Flouresbrite YG microspheres (Polysciences Inc, PA) diluted 1:4 in sterile PBS in mice undergoing regression 3 days prior to harvest ...
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bioRxiv - Immunology 2022Quote: ... and packaging plasmid psPax2 and envelope plasmid pMD.2G with polyethylenimine (PEI) (at a ratio of 1:2.3 DNA:PEI) (Polysciences, catalog no. 24765-2) in Optimem (Life Technologies Europe BV ...
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bioRxiv - Immunology 2022Quote: ... using polyethylenimine (PEI, Polysciences). The supernatant was harvested 5 days after transfection ...
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bioRxiv - Immunology 2022Quote: ... The S2P or S6P pCMV plasmids were used to transiently transfect HEK293F cells with polyethylenimine (PEI) (Polysciences, #24765). The recombinant S6P was affinity purified from the cell supernatant by using StrepTactin resin (IBA) ...
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bioRxiv - Immunology 2022Quote: ... and mixed with 20 μl IMDM medium containing 1.2 μl of 1 mg/mL polyethylenimine (PEI, Polysciences). Following a 20 min incubation step at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Vero/TMPRSS2 cells were transfected with an ACE2-targeting plasmid using PEI MAX (Polysciences). At 24 h posttransfection ...
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bioRxiv - Microbiology 2022Quote: ... Vero/TMPRSS2 cells were transfected with pCAGGS-blast-RcACE2 or pCAGGS-blast-hACE2 plasmids using the PEI MAX transfection reagent (Polysciences, Warrington, PA, USA). Transfected cells were treated with 10 μg/mL blasticidin S (Kaken Pharmaceutical ...
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bioRxiv - Developmental Biology 2022Quote: ... with PEI reagent (Polysciences, 23966-1). Lentiviral particles were collected 48 h and 72 h after transfection and filtered.
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bioRxiv - Immunology 2022Quote: ... pH 7 (Polysciences) to transfect 293Freestyle cells ...
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bioRxiv - Immunology 2022Quote: ... A549-ACE2-A2402 cells (3 × 105 cells) were transfected with 2 μg of plasmids expressing prototype spike or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Expression and production of nanobodies were conducted by transfecting the expression vectors into the HEK293F cells using polyethyleneimine (PEI) (Polysciences). After approximately 96 h ...
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bioRxiv - Cell Biology 2022Quote: ... the displacement of 0.5 µm fluorescent beads in the SPP growth media (Polysciences Inc; 18339) was imaged using a 20x objective lens (Ti Eclipse ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were maintained in FreeStyle 293 medium and transfected using the PEI Max reagent (Polysciences Inc.) with a 1:3 ratio (w/w ...
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bioRxiv - Cell Biology 2022Quote: Cells were fixed with 4% paraformaldehyde (PFA) (Polysciences, Warrington, United States). Unspecific binding sites were blocked by incubation with blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... we used Aquapolymount (Polysciences) to mount onto glass slides ...
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bioRxiv - Cancer Biology 2022Quote: ... using PEI (Polysciences, Inc; 23966100) in a 1:3 ratio (total DNA:PEI) ...
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bioRxiv - Developmental Biology 2022Quote: ... and Pico-Sirius Red solution (Polysciences, 24901) as previously described[19].