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Citations for Polysciences :
1601 - 1650 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... RIKEN BRC) [22] were mixed with 111 µl of 1 mg/ml PEI MAX® (pH7.5) (Cat. No. 24765-1, Polysciences) in Opti-MEM™ I Reduced Serum Medium (Cat ...
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bioRxiv - Cell Biology 2022Quote: ... Larvae were fixed in 4% paraformaldehyde (Laborimpex, 15714) and 2.5% glutaraldehyde (Polysciences, Inc, 111-30-8) in 0.1 M sodium-cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Cell Biology 2022Quote: Adult fly heads were decapitated and immediately fixed in 4% paraformaldehyde (Laborimpex, 15714) and 2% glutaraldehyde (Polysciences, Inc, 111-30-8) in 0.1 M Na-Cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Plant Biology 2022Quote: ... and then embedded in Spurr’s resin (Polysciences). We cut the Spurr’s blocks into semi-thin sections (0.75–2 μm ...
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bioRxiv - Biochemistry 2022Quote: ... Transfection reactions were prepared by the addition of 2-10 ug of bacmid DNA to 100 μl 1xPBS and 12 μL polyethyleneimine (PEI) at 1 mg/mL (Polyethyleneimine “Max” MW 40.000, Polysciences #24765, USA) to 100 μL 1xPBS ...
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bioRxiv - Immunology 2022Quote: ... alone (control) or with Fluoresbrite® Yellow-Green carboxylate microspheres (1 μm) (Polysciences Inc., 15702) in a ratio of 3:1 beads-to-cells ...
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bioRxiv - Microbiology 2022Quote: ... Samples were washed in phosphate buffer and post-fixed in 1% osmium tetroxide (Polysciences Inc., Warrington, PA) for 1 hr ...
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bioRxiv - Microbiology 2022Quote: ... samples were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100mM PIPES/0.5mM MgCl2 ...
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bioRxiv - Microbiology 2022Quote: ... infected cells were fixed in 4% paraformaldehyde/0.05% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100mM PIPES/0.5mM MgCl2 ...
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bioRxiv - Developmental Biology 2022Quote: ... Exogenous plasmid DNA was transfected using Transporter™ 5 transfection reagent (Polysciences, PA, USA). Co-transfection of siRNA with plasmid DNA was performed using jetPRIME® transfection reagent ...
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bioRxiv - Developmental Biology 2022Quote: ... and plasmids were transfected using polyethylenimine (PEI; Polysciences, Warrington, PA, USA) or Fugene 6 (Promega ...
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bioRxiv - Developmental Biology 2022Quote: ... purified plasmid was mixed with linear 25 kDa PEI (Polysciences) at a ratio of 1 μg DNA:2.2 μg PEI (per 5 × 106 cells ...
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bioRxiv - Neuroscience 2022Quote: ... in 1μl polystyrene beads (100 nm diameter; Polysciences, Inc.) for their mechanical restraint ...
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bioRxiv - Developmental Biology 2022Quote: ... A drop of Halocarbon 200 Oil (Polysciences; for DIC experiments) or 1x phosphate buffered saline (prepared from Dulbecco’s phosphate buffered saline (eurobio) ...
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bioRxiv - Immunology 2022Quote: ... were diluted with 150 mM NaCl to a concentration of 40 μg/mL and mixed with 40-kDa polyethyleneimine (PEI, Polysciences, Warrington, PA, USA) (DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were washed 3x 20 minutes in 1% Triton X-100 PBS at room temperature and tissues preserved under coverslips using Aqua-Poly/Mount (Polysciences, 18606). The following primary and secondary antibodies at indicated concentrations were used ...
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bioRxiv - Microbiology 2022Quote: ... MW∼25,000 (Polysciences, Inc. Cat#23966) at a ratio of 1µg plasmid DNA ...
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bioRxiv - Bioengineering 2022Quote: Red and green fluorescent microspheres (Polysciences: YG Microspheres ...
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bioRxiv - Biochemistry 2022Quote: ... via Polyethylenimine PEI 25K (Polysciences, 23966-1) and V0 virus were subsequently amplified.
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bioRxiv - Biochemistry 2022Quote: ... purified plasmid DNA was mixed with PEI MAX (PolySciences Inc.) in a 1:2.5 (w/w ...
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bioRxiv - Biochemistry 2022Quote: HEK-293T cells were transiently transfected with plasmid DNA using polyethylenimine (Polysciences) at a ratio of DNA 1:3.4 polyethylenimine ...
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bioRxiv - Molecular Biology 2022Quote: L1 and L4 larvae were mounted on a 10% agarose pad and immobilized in a 1-2 μL suspension of 0.1 μm polystyrene beads (Polysciences), as described in (Kim et al ...
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bioRxiv - Microbiology 2022Quote: ... The cells were transfected by using polyethyleneimine (PEI) transfection reagent (Polysciences). Transfected cells were further incubated at constant shaking speed for 4 days before harvesting the culture supernatant ...
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bioRxiv - Microbiology 2022Quote: ... cells were transfected with 100 ng of human or rhesus ACE2 receptor plasmid DNA using polyethylenimine (Polysciences). After 24 h ...
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bioRxiv - Biophysics 2022Quote: ... The transfection solution was prepared by combining 5 μL of 1 mg/ml polyethylenimine Max solution (Polysciences) and a plasmid mixture consisting of 200 ng ssHA-FLAG-NTS1 and 500 ng alkaline phosphatase (AP)-tagged TGF-α (AP-TGFα ...
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bioRxiv - Biophysics 2022Quote: ... The cells in each well were transiently transfected with 1 μg HcKCR1/2-CGFP DNA plasmid using 3μg linear polyethylenimine (PEI, MW 25000, Polysciences) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and covers were mounted with Aqua-Poly/Mount solution (cat#18606-5 from Polysciences Europe) on glass slides ...
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bioRxiv - Cell Biology 2022Quote: All transfections were performed by mixing DNA and polyethylenimine (PEI, Polysciences, 23966) in a 1:3 ratio (w/w ...
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bioRxiv - Cell Biology 2022Quote: 200,000 HeLa cells/35-mm well were seeded onto coverslips (Marienfeld, 630-2190) and transfected using polyethylenimine transfection reagent (PEI, Polysciences, 23966) in a 1:6 (w/w ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were then washed 3 × 30 mins in PBS and then immersed through a gradient of sucrose solutions from 10%-50% at 4°C for > 4 hrs each before being embedded in cryomolds (Polysciences 18646A) using OCT (Sakura Finetek ...
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bioRxiv - Cancer Biology 2022Quote: ... rinsed and mounted with Aqua Polymount (Polysciences 18606).
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bioRxiv - Developmental Biology 2022Quote: ... and bound to activated Concanavalin A beads (Polysciences) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed with 4 % paraformaldehyde (Polysciences) and stained with the antibody anti-mouse KI67 ...
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bioRxiv - Cell Biology 2022Quote: ... animals were soaked in ScaleA2 (4M urea, 20% glycerol, 0.1% Triton X-100, 2.5% DABCO)38 and mounted in Aqua-Polymount (Polysciences Inc. 18606). Some in situ hybridizations were carried out in a CEM InSituPro Hybridization robot up to the development stage ...
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bioRxiv - Bioengineering 2022Quote: ... poly(ethylene glycol) diacrylate (PEGDA) with a molecular weight of 4000 Da (Polysciences), visible-light lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were mounted in Aqua-Poly/Mount (Polysciences, Hirschberg, Germany). Control experiments without primary antibodies indicated absence of unspecific labeling except for the goat-anti-mouse secondary antibody that labeled blood vessels (not shown) ...
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bioRxiv - Cell Biology 2022Quote: ... For large scale transfections of the adherent HEK293T cells for the ectopic expression of proteins to be used for the NB in vitro assembly experiments, we followed a transfection protocol (Reed et al, 2006) using linear 25 kDa polyethylenimine (PEI; Polysciences Europe GmbH, Hirschberg, Germany). We adjusted this protocol for 10 cm culture dishes with about 50-70% cell density ...
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bioRxiv - Neuroscience 2022Quote: ... and transfer plasmids using polyethylenimine MAX (Polysciences, 24765). The cell pellets were suspended in 1X Gradient Buffer (10mM Tris-HCl pH=7.6 ...
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bioRxiv - Neuroscience 2022Quote: ... cells at ∼70% confluency were transfected using Polyethylenimine (PEI, Polysciences. Warrington, PA, USA) with second-generation lentiviral packaging vectors (psPAX2 and 2MD2.G ...
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bioRxiv - Synthetic Biology 2022Quote: ... pMDLg/pRRE) and 8 ug of rTetR-concatenation library vectors using 50 mL of polyethylenimine (PEI, Polysciences #23966). Packaging plasmids were gifts from Didier Trono ...
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bioRxiv - Biophysics 2022Quote: ... using PEI (Polysciences). Viruses were collected at 48 hrs post-transfection ...
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bioRxiv - Microbiology 2022Quote: ... and 500 ng plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2022Quote: ... and 750ng of lentiviral transfer plasmid encoding the transgene of interest after a 15 minute incubation of these plasmids with 10μL of polyethylenimine (PEI, Polysciences #23966). Lentivirus-containing culture supernatant was harvested 72 hours after transfection ...
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bioRxiv - Neuroscience 2022Quote: ... Polyethylenimine (PEI MAX; Polysciences, Cat#24765), Fluoromount-G Mounting Medium (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... 170 ng of expression vectors encoding androgen receptor (AR) tagged with eGFP (eGFP-AR) or mutants were transfected per well using polyethylenimine (PEI) (Polysciences) at a ratio of 1 µg DNA to 3 µl PEI ...
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bioRxiv - Biochemistry 2022Quote: ... and the resulting plasmids were transfected into HEK293F cells using polyethylenimine (PEI, Polysciences) for expression.
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bioRxiv - Molecular Biology 2022Quote: ... each 6 cm dish of cells was transfected with 2-12 μg of poly(ethylenimine) (Polysciences, Eppelheim, Germany) and 1-6 μg of plasmid DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293T cells were transfected with plasmids using polyethylenimine (PEI; Polysciences Inc.) at PEI:DNA ratio 2.5:1 ...
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bioRxiv - Cell Biology 2022Quote: ... washed thrice with 1X PBS before mounting with Aqua-Poly/Mount (Polysciences).
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bioRxiv - Cell Biology 2022Quote: ... with polyethylenimine (Polysciences) for AD-293 or Lipofectamine 2000 (ThermoFisher Scientific ...