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Citations for Polysciences :
1851 - 1900 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and 1% osmium tetroxide (Polysciences Inc.) in 50 mM phosphate buffer at 4ºC for 30 min ...
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bioRxiv - Microbiology 2022Quote: HIV virus was produced by transient transfection of HEK293T cells using Polyethyleneimine (PEI, 1 mg/ml, linear, MW 25000, Polysciences Inc.). Pseudotyping of GFP-HIV (HIV-1 NL4-3 ΔEnv EGFP Reporter Vector ...
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bioRxiv - Microbiology 2022Quote: Leishmania promastigotes were harvested in logarithmic growth phase and fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM phosphate buffer ...
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bioRxiv - Microbiology 2022Quote: ... Samples were washed in phosphate buffer and postfixed in 1% osmium tetroxide (Polysciences Inc., Warrington, PA) for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... pLV-EGFP-luciferase-N and psPAX2 plasmid using polyethyleneimine (PEI, Polysciences, USA). Supernatants were harvested at 48 h and 72 h ...
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated at 37°C for 3 days and later fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Microbiology 2022Quote: ... 200 μl of 4% PFA (Polysciences) was added to the upper chamber of the Transwells and incubated for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... Poly(ethylenimine) (PEI) (Polysciences) transfected cells were incubated for 48 h at 33 °C ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg pcDNA3.1- hsa_circ_0001400 of plasmids was transfected to 2×106 cells with 12 μl Transporter 5 (Polysciences) lipofection reagent and 1 ml Opti-MemI in 10 ml DMEM ...
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bioRxiv - Microbiology 2022Quote: Soluble PCDH1 variants cloned into pcDNA3.1 (see above) were expressed in 293F cells in shaker flasks by transient transfection with linear polyethyleneimine (Polysciences), as described previously (56) ...
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bioRxiv - Microbiology 2022Quote: ... using polyethylenimine (Polysciences). All downstream experiments were performed 24 h post-transfection ...
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bioRxiv - Pathology 2022Quote: ... and coverslipped with aqueous mounting medium (Polysciences, Inc.).
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bioRxiv - Systems Biology 2022Quote: ... pMDLg/pRRE) and 8 ug of rTetR-domain library vectors using 50 mL of polyethylenimine (PEI, Polysciences #23966). pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Systems Biology 2022Quote: ... 3ul of 1mg/ml PEI-MAX transfection reagent (Polysciences) was added per ug of total plasmids ...
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bioRxiv - Plant Biology 2022Quote: 2.5 % (v/v) glutaraldehyde (Polysciences, Inc., Warrington, USA), and were kept refrigerated until the start of embedding process on the ground (Fig ...
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bioRxiv - Plant Biology 2022Quote: ... 1.5 mg plasmids and 4 mg inear polyethylenimines (PEI) (Polysciences) were pre-incubated in 50 ml fresh medium for 30 min before adding into one litre cells at density of 3.0 × 106 cells ml−1 ...
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bioRxiv - Neuroscience 2022Quote: ... cell nuclei were stained by DAPI for 1 min at RT and tissues were mounted in Aqua-Poly/Mount (Polysciences, #18606). Images were acquired using Zeiss LSM 880 confocal microscope with a Zeiss 20x/0.8 Plan-Apochromat objective and later analyzed using ImageJ.
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bioRxiv - Neuroscience 2022Quote: ... pAAV2-SynTetOff-mRFFP1-BGHpA and helper plasmids were transfected into HEK293T cells (RCB2202, RIKEN BioResource Research Center) with polyethylenimine (23966, Polysciences). Virus particles collected from the cell lysate and supernatant were purified by ultracentrifugation with OptiPrep (1114542 ...
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bioRxiv - Neuroscience 2022Quote: ... using polyethylenimine (Polysciences). Cells were harvested and pelleted in a benchtop centrifuge 60 h after transfection and the supernatant discarded ...
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bioRxiv - Neuroscience 2022Quote: ... MW 25000 (‘PEI’, Polysciences) resuspended to 1mg/mL in H2O at a 3:1 (v/w ...
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bioRxiv - Plant Biology 2022Quote: ... The specimen was washed with acetone and embedded in Spurr resin (Spurr Low Viscosity Embedding Kit, Polysciences). Ultra-thin sections (70–80 nm ...
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bioRxiv - Neuroscience 2022Quote: ... and coverslipped using Aqua Polymont (Polysciences, Inc., Warrington, USA). The slides were stored protected from light at 4°C until analysis.
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bioRxiv - Neuroscience 2022Quote: ... Sections were then mounted on slides with Aqua-Poly/Mount (Polysciences, #18606). Images were acquired using a Zeiss LSM 800 confocal microscope with Zeiss 10x/0.45 Plan-Apochromat and Zeiss 20x/0.8 Plan-Apochromat objectives and analyzed using ImageJ (National Institute of Health) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were transfected using polyethyleneimine (PEI, Polysciences, Germany) and 150 mM NaCl using a 1:6 (w:v ...
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bioRxiv - Neuroscience 2022Quote: Linear polyethylenimine (PEI, MW 25000; Polysciences Inc, #23966-2) was dissolved in 1× PBS (pH 4.5 ...
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bioRxiv - Neuroscience 2022Quote: ... Fluoresbrite YG Microspheres (0.20 μm size; Polysciences Inc.) were diluted in distilled water and pipetted on the coverslip ...
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bioRxiv - Neuroscience 2022Quote: ... mEGFP positive slices were identified and mounted on cover slips with Aqua Polymount (Polysciences, Inc., Warrington, PA, USA). Confocal images were acquired with a Zeiss LSM 700 or 880 confocal scanning microscope using a 63x/1.3 NA Apochromat multi-immersion objective ...
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bioRxiv - Microbiology 2022Quote: ... cells using polyethylenimine (PEI) (Polysciences, Inc.) to generate the lentiviral particles ...
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bioRxiv - Neuroscience 2022Quote: ... JetPEI (Polysciences) was used as transfection reagent ...
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bioRxiv - Neuroscience 2022Quote: ... Coverslips were then coated with yellow green fluorescent fiducial beads (Polysciences, 17149-10) in PBS for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue was cover slipped under Aqua-Poly Mount (Polysciences, Warrington, USA) and stored at 4°C in the dark.
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bioRxiv - Neuroscience 2022Quote: ... Slides were cover slipped using Aqua-Poly Mount (Polysciences).
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bioRxiv - Neuroscience 2022Quote: ... The seeded cells were then transfected with a total 2 μg of DNA using polyethylenimine 25 kDa (PEI, Polysciences Inc., Germany) at a 6:1 ratio of PEI to DNA ...
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bioRxiv - Physiology 2022Quote: ... C3H10T1/2 cells were transfected with Transporter 5® Transfection Reagent (Polysciences) following manufacture’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Slides were mounted with Aqua-Poly/Mount (Polysciences #18606-20) and scanned with a Pannoramic 250 slide scanner at 40 x magnification.
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bioRxiv - Microbiology 2022Quote: ... 4% (v/v) electron microscopy-grade formaldehyde (PFA, Polysciences) for 10 min on ice before and then incubated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... + 7μL 0.05um polybeads (2.5% solids w/v, Polysciences, Inc. #15913-10) on a 10% agarose pad.
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bioRxiv - Neuroscience 2022Quote: ... + 7μL 0.05um polybeads (2.5% solids w/v, Polysciences, Inc. #15913-10). Single-plane snapshots were collected at 1 fps using triggered acquisition ...
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bioRxiv - Neuroscience 2022Quote: ... and 7μL 0.05um polybeads (2.5% solids w/v, Polysciences, Inc. #15913-10). Single plane movies were captured at 0.5fps (frames per second ...
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bioRxiv - Neuroscience 2022Quote: ... + 7μL 0.05um polybeads (2.5% solids w/v, Polysciences, Inc. #15913-10) on a 10% agarose pad ...
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bioRxiv - Neuroscience 2022Quote: ... and 7μL 0.05um polybeads (2.5% solids w/v, Polysciences, Inc. #15913-10)] ...
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bioRxiv - Synthetic Biology 2022Quote: ... were transfected with pHelper, pRepCap and pTransgene (2:1:1 ratio, 2 μg DNA per million cells) using Transport 5 transfection reagent (Polysciences) with a 2:1 PEI:DNA ratio ...
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bioRxiv - Immunology 2022Quote: ... expi293F cells were transfected with antibody expression plasmids at a density of 2.5 million cells per/ml using 1 mg/ml PEI-Max transfection reagent (Polysciences). Supernatants were harvested 4-5 days post-transfection and tested for their SARS-CoV-2 RBD binding potential by enzyme-linked immunosorbent assay (ELISA) ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transfected with 100 ng/well of pcDNA 3.1 Myc/His empty vector or MT1-MMP plasmid DNA using PEI max transfection reagent (Polysciences, catalog no. 24765). After 6 hours of treatment ...
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bioRxiv - Microbiology 2022Quote: ... we used the ratio of the median forward scatter of each cell population to that of the median forward scatter of reference beads (Polysciences™ ...
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bioRxiv - Microbiology 2022Quote: ... the culture medium was removed and the cells transfected with pCMV-ORF45HA or pEGFP-C1 (Mock control) using Polyethylenimine (PEI) transfection reagent (Polysciences, Inc.). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... PLGA NPs were coated with branched PEI (Polysciences, #06090) by reaction with EDC (Thermo Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... or PEI (Polysciences PEImax) following manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: ... We then rinsed the samples with pure acetone before infiltration of the resin lowicryl HM20 (Polysciences). The resin was polymerized with UV at -25 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... Fixed samples were gradually dehydrated in an increasing series of ethanol concentrations and were embedded in a methacrylate resin (JB-4 kit, Polysciences, Warrington, PA) containing eosin and acridin orange as contrasts agents as previously described (Mohun and Weninger ...