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Citations for Polysciences :
1501 - 1550 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... fixed with 4% formaldehyde (FA; 18814; Polysciences) for 12 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... using PEI 25K transfection reagent (Polysciences Inc, Cat. 23966-1), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Transfection was carried out using preheated (70°C) 40 kDa polyethyleneimine (PEI-MAX) (Polysciences) at 1mg of plasmid DNA per 1 liter of culture once cell density has reached 1*106 cells/ml ...
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bioRxiv - Microbiology 2022Quote: ... Prochlorococcus cell sizes were normalized to 2 µm beads (Polysciences). Cells were excited with a blue 488 nm laser and analyzed for red fluorescence (692/40 nm) ...
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bioRxiv - Microbiology 2022Quote: ... washed in 0.1% saponin in PBS then mounted onto glass slides using Aqua-Poly/Mount (Polysciences, Inc.). See Table S3 for information on primary antibodies and dilutions used ...
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bioRxiv - Developmental Biology 2022Quote: ... The CSII-CMV-MCS-IRES2-Bsd vector (Fbln7 FL, dCC or control eGFP) was transfected into 293T cells using polyethylenimine reagent (PEI, Polysciences Inc.) together with lentivirus packaging vectors (pRSV-Rev ...
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bioRxiv - Microbiology 2022Quote: ... carrying defective envelope of VSV plasmid (trans form) and GFP reporter plasmid by using Transporter TM5 transfection reagent (PEI, 40,000Da, PolySciences), according to the provider guidelines ...
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bioRxiv - Molecular Biology 2022Quote: ... using PEI Max (Polysciences) and expressed at 37°C for 7 days ...
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bioRxiv - Physiology 2022Quote: ... animals were mounted on 2 % agarose dry pads with 4 % polystyrene beads (Polybeads, Polysciences) in M9 buffer ...
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bioRxiv - Neuroscience 2022Quote: ... At 18 DIV Cells were fixed using 4 % PFA for 15 min and mounted on glass slides using Aqua-Poly/Mount (Polysciences Inc., Eppelheim). The experimenter was blinded to all the conditions ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... fixed in 10% neutral buffered formalin (Polysciences) at room temperature for 48 h ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then stained with 5 µg/ml DAPI (4′,6-diamidino-2-phenylindole) for two minutes to visualize nuclei and mounted in Mowiol mounting medium (Polysciences) in glycerol containing 2.5% 1,4-diazabicyclo-(2,2,2)-octane (DABCO ...
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bioRxiv - Cell Biology 2022Quote: ... and polyethyleneimine (PEI; Polysciences, Warrington, Pennsylvania) were used for transient transfection of short interfering RNA (siRNA ...
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bioRxiv - Cell Biology 2022Quote: All transfections were done by incubating DNA with the transfection reagent polyethylenimine (PEI, 25K linear, Polysciences 23966) in a 1:3 ratio in serum free DMEM ...
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bioRxiv - Cancer Biology 2022Quote: ... Transfections were performed using PEI MAX (Polysciences) for all cell lines apart from Jurkat and RD for which Lipofectamine LTX was used ...
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bioRxiv - Cancer Biology 2022Quote: ... 15.12 µl of PEI Max Transfection Grade Linear Polyethylenimine Hydrochloride MW 40.000 (Polysciences) (1 mg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... HEK-293 cells were co- transfected with 3μg of either V2RWT or V2RT360A along with 2μg of βarr1-mYFP in presence or absence of 2μg of Ib30 with help of polyethylenimine (Polysciences; cat. no. 23966) reagent (21μl ...
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bioRxiv - Biochemistry 2022Quote: ... media was changed for DMEM high glucose supplemented with only 2.5% FBS and cells were transfected using PEI (Polysciences; cat. no. 23966) as follow ...
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bioRxiv - Cancer Biology 2022Quote: ... using polyethylenimine (Polysciences). Fresh media was added 24 hours later and viral supernatant harvested 24 hours after that ...
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bioRxiv - Cancer Biology 2022Quote: Calculation of absolute cell number was performed by adding to each vial a fixed number (10,000) of nonfluorescent 10 μm polybead® carboxylate microspheres (Polysciences, IL, USA) according to the formula ...
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bioRxiv - Microbiology 2022Quote: ... and 100 ng of the G2-tagged targets (CTD, RPB1 or RPB2, respectively) using polyethyleneimine (PEI-max, #24765-1 Polysciences Inc). When indicated ...
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bioRxiv - Developmental Biology 2022Quote: ... Live adults were placed in a drop of 1 uL H2O and 1 uL polystyrene microspheres (Polysciences, Inc., #00876) and then immobilized on 6% agarose pads ...
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bioRxiv - Microbiology 2022Quote: ... Samples were washed in sodium cacodylate buffer and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 hr ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
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bioRxiv - Immunology 2022Quote: ... Constructs were transfected into Expi293 cells (Thermo Fischer Scientific) using Polyethyleneimine “Max” (Polysciences). Cell cultures were maintained in Expi293 Media (Thermo Fischer Scientific ...
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bioRxiv - Immunology 2022Quote: ... 293T cells were seeded in T175 flasks and transfected using polyethylenimine (PEI, Polysciences) with F4.HSA proviral DNA (70 μg / flask ...
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bioRxiv - Microbiology 2022Quote: ... PVSA (poly(vinylsulfonic acid) was acquired from Polysciences. The following reagents were acquired from Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were transfected with the appropriate SARS-CoV-2 Spike gene expression vector (Wuhan, Alpha, Delta, or Omicron) together with p8.9171 and pCSFLW72 using polyethylenimine (PEI, Polysciences, Warrington, USA). HIV (SARS-CoV-2 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Sections were washed again 3 times for 20 minutes each time before mounting onto slides with AquaPoly mounting media (18606, Polysciences). Mounted sections were stored at 4°C in dark slide boxes until image processing.
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... a 20 μl droplet of 1 μm diameter Polybead® Carboxylate Red Dyed Microspheres (Polysciences Inc ...
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bioRxiv - Bioengineering 2022Quote: ... and 20 μm FITC (Polysciences Inc., Warrington, USA) particles were suspended in deionized water to make up a final concentration of 1 × 106 beads/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... All reagents were from Sigma-Aldrich except uranyl acetate (Polysciences, Philadelphia).
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bioRxiv - Bioengineering 2022Quote: ... fluorescent polystyrene beads (Polysciences) with 350 μL of the gelatin-mTG solution to aid in visualization and added them to dishes that had a 20 mm glass bottom (MatTek Corporation) ...
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bioRxiv - Bioengineering 2022Quote: ... Masson’s Trichrome (Polysciences, Warrington, PA) stain was used to identify collagen and muscle ...
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bioRxiv - Bioengineering 2022Quote: LPEI (molecular weight 250,000) was purchased from Polysciences Inc ...
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bioRxiv - Bioengineering 2022Quote: ... Defects were filled with 30 mg of (poly)caprolactone (particulate, Mn=50,000 g/mol, mean particle size < 600um, Polysciences), decellularized extracellular matrix (Matristem ...
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bioRxiv - Biochemistry 2022Quote: ... The two constructed pSpCas9(BB)-2A-Puro (PX459) vectors against individual genes were transiently transfected into HCT116 cells in a six-well plate using polyethyleneimine “Max” (Polysciences, Inc.). At 48 h post-transfection ...
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bioRxiv - Biochemistry 2022Quote: ... cells were grown overnight and transfected using polyethyleneimine “Max” (Polysciences, Inc.) as previously described [20].
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bioRxiv - Neuroscience 2022Quote: ... slices were mounted with Aqua-Poly/Mount (Polysciences) mounting media.
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bioRxiv - Cancer Biology 2022Quote: ... Addgene)[70] in a ratio of 5:3:2 using polyethylenimine (24765-2, Polysciences). Virus supernatant was harvested 30 h after transfection ...
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Methacrylic acid-based biomaterials promote peripheral innervation in the subcutaneous space of micebioRxiv - Bioengineering 2022Quote: ... and sodium polymethacrylate (PMAA-Na) (100 kDa; Polysciences) with dithiothreitol (DTT ...
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bioRxiv - Cell Biology 2022Quote: ... pFLAG-CMV5a-ABCF1-V5 were co-transfected with control empty pFLAG-CMV5a vector or pFLAG-CMV5a-SOX15 into 293T using polyethylenimine (PEI, Polysciences) at a DNA:PEI (μg ...
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bioRxiv - Neuroscience 2022Quote: ... Coverslips were finally mounted with 10% Mowiol 4-88 (Polysciences, Warrington, PA) dissolved in 0.1 M Tris-HCl (pH 8.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... and pLP/VSVG (2 µg) (clontech) using Polyethylenimine (PEI) Max (Polysciences). Virus containing supernatant was collected 48 h after transfection and cleared through a 0.45-µm PDF filter ...
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bioRxiv - Cancer Biology 2022Quote: ... Picrosirius Red stain Kit (Polysciences Inc., PA, USA) was used with a slight modification of the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... transfection reagent or Polyethylenemine (Polysciences Inc.) at a 3:1 transfection reagent to DNA ratio ...
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bioRxiv - Cell Biology 2022Quote: ... cells were pre-permeabilised in 0.5% Triton X-100 in cytoskeleton buffer for 15 s and then fixed in 0.5% glutaraldehyde (no.00216-30; Polysciences) in cytoskeleton buffer with 0.5% Triton X-100 and 10% sucrose for 15 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescent beads (1 μm, Polysciences) were added for internal calibration (105 mL−1) ...
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bioRxiv - Microbiology 2022Quote: ... An in-house calibration was applied to transform relative SSC values from Polysciences-bead-referenced to Molecular-Probes-bead-referenced (Relative SSCMP = 2.201 × Relative SSCPS) ...
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bioRxiv - Developmental Biology 2022Quote: ... the testes were embedded in JB4 embedding medium (Polysciences Inc., Warrington, PA, USA). Transverse sections through the testes were collected using Leica RM2235 microtome and stained with hematoxylin and eosin ...