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Citations for Polysciences :
1401 - 1450 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Polyethylenimine (PEI, Polysciences) was used to transfect HEK293T cells for StrepTactin protein purification and streptavidin pull-down experiments ...
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bioRxiv - Immunology 2022Quote: ... the pCAGGS-RBD plasmid was transfected into ∼5×107 adherent HEK-293/T17 cells (ATCC CRL-11268) in a T-175 using PEI (Polyethylenimine, linear 25,000 m.w.(Polysciences, Warrington, PA, USA). Plasmid was mixed at a 1:3 ratio with PEI (20ug of plasmid ...
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bioRxiv - Microbiology 2022Quote: ... and the plasmid containing a gene of interest or hairpin using polyethylimine (PEI, Polysciences). psPAX2 was a gift from Didier Trono (Addgene plasmid #12260 ...
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bioRxiv - Developmental Biology 2022Quote: ... Fluorescent polychromatic beads (Polysciences, 19507-5) with a 0.5 μm particle size were added to each well at a final concentration of 3.64×108 beads/mL and incubated at 20°C for 10 minutes with shaking ...
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bioRxiv - Microbiology 2022Quote: ... and the lentiviral transfer plasmid containing a gene of interest using polyethylenimine (PEI, Polysciences). 6 hours after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... polyethylenimine (PEI; 24765, Polysciences), complete EDTA-free protease inhibitor cocktail (11697498001 ...
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bioRxiv - Bioengineering 2022Quote: ... linear polyethylenimine (PEI; Polysciences, Inc). In each plate ...
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bioRxiv - Bioengineering 2022Quote: ... 2 µg/ml linear 25 kDa polyethylenimine (Polysciences), and OptiPRO SFM medium (4% final concentration ...
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bioRxiv - Bioengineering 2022Quote: ... A concentration of 2 μg mL-1 plasmid DNA and 2 μg mL-1 plasmid DNA linear 25kDa polyethyleneimine (Polysciences) dissolved in OptiPRO serum free medium (Thermo Fisher ...
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bioRxiv - Biophysics 2022Quote: ... Carboxylated polystyrene microspheres (0.5 µm in diameter; 2.5% solids) were purchased from Polysciences.
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were cultured in T75 flasks and transfected at approximately 70% confluency with 7500 ng of the lentiviral vector of interest and packaging plasmids pCMV-dR8.91 (6750 ng) and pCMV-VSV-G (750 ng) with 75 µL of polyethyleneimine (PEI, 1 mg/mL; Polysciences). Approximately 72 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... 2 × 107 1µm YG-labelled polystyrene beads (Polysciences 15702-10) were then added ...
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bioRxiv - Biophysics 2022Quote: ... 0.2% glutaraldehyde (Polysciences)] in a gradient master (BioComp) ...
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bioRxiv - Cell Biology 2022Quote: ... 20µl of cell suspension was spotted onto each well of a Teflon coated 10-well slide (Polysciences, 18357) that had been pre-treated with 10 ng/µl poly-L-lysine ...
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bioRxiv - Cell Biology 2022Quote: ... After 6 h of LPS treatment 16% Formaldehyde (18814-20, Polysciences Inc.) was added to the medium in the dish to a concentration of 1% and incubated (gently mixing ...
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bioRxiv - Cell Biology 2022Quote: ... followed by fixation using 4% paraformaldehyde (18814-20, Polysciences Inc.) in 1X PBS for 20 min ...
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bioRxiv - Cell Biology 2022Quote: ... 150 mm dishes of HEK293T cells (∼80% confluent) were transfected with 10 μg of plasmid using 60 μg of linear Polyethylenimine 25 kDa (23966, Polysciences Inc) mixed in 2 mL Opti-MEM (31985070 ...
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bioRxiv - Cell Biology 2022Quote: ... worms were mounted on 8–10% agarose pads in 3 μL of a 0.5 μM polystyrene bead slurry (Polysciences, Warrington, PA) in PBS pH 7.4 (Kim et al. ...
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bioRxiv - Cell Biology 2022Quote: ... washed again and mounted with Aqua-Poly/Mount (Polysciences). The following antibodies were used ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3μl of 1.25% (w/v) polystyrene microspheres (Polybead Microspheres 0.05μm, Polysciences) and 0.2mM levamisole (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with 500 ng of GFP-LRRK2 construct and 500 ng of GFP-Rab10 using polyethylenimine (PEI, Polysciences). After 36 h ...
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bioRxiv - Cell Biology 2022Quote: ... the polystyrene beads containing surface primary amino groups (PolySciences, 17145-5, Diameter 3 μm) were incubated with 10 mM of Sulfo-NHS-LC-LC-Biotin (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were mounted using 10% (wt/vol) Mowiol 4-88 (Polysciences). Epifluorescent images were captured using a Nikon Eclipse NiE (40×/0.75 Plan Fluor Nikon objective ...
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bioRxiv - Developmental Biology 2022Quote: ... Plat-E cells33 seeded on a 10cm-culture dish were transfected with the vector plasmid using PEI Max (Polysciences, 24765) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... plasmids were transfected with polyethyleneimine (PEI; Polysciences, Cat# 23966) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2022Quote: ... 50% 0.1 micron polystyrene Microspheres (Polysciences Inc.), and covered with a coverslip ...
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bioRxiv - Developmental Biology 2022Quote: ... the testes were mounted on coverslip using Vectashield (VectorLabs) or Aquapolymount (Polysciences, Inc). The following are the concentration of primary antibodies used for immunolabeling in this study ...
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bioRxiv - Developmental Biology 2022Quote: ... and mounted in 50%PBST 50%Aqua-Poly/Mount (Polysciences) for confocal imaging.
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bioRxiv - Developmental Biology 2022Quote: ... TrpA1-KI-Flag and TrpA1 mutant (different days as specified in legend) were fixed in 4% Formaldehyde (Polysciences) in 1XPBS for 2 hours under room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Transfection was done with 3 μg polyethylenimine (Polysciences) per 3.5 cm dish 1 day after plating ...
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bioRxiv - Developmental Biology 2022Quote: Images recorded by the lightsheet microscope were registered based on the position of fiduciary beads embedded in the agarose (Fluoresbrite multifluorescent 0.5-μm beads 24054, Polysciences Inc., as described in Ref. [26]) using the Multiview reconstruction plugin [40] in Fiji [41] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... following by curing the resin overnight at 4°C in a plastic embedding mold (Cat no.-15899-50, Polysciences Europe GmbH, Germany). Polymerized sample blocks were further hardened by overnight baking at 70°C.
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bioRxiv - Immunology 2022Quote: ... and pMD2G-VSV-G (envelope) using PEI-MAX (Polysciences). Packaged lentivirus was used to transduce 293T cells in the presence of 5μg/mL polybrene ...
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bioRxiv - Microbiology 2022Quote: ... A coverslip was mounted with AquaPoly mounting solution (Polysciences). Confocal images were acquired using a Leica SP8 scanning confocal microscope controlled by Leica LAS-X software ...
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bioRxiv - Molecular Biology 2022Quote: ... and pVSVG using PEI MAX (Polysciences, Warrington, PA, USA) to prepare the lentiviral vectors ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected using 90 μg of PEI-Max (1 mg/mL, Polysciences) with ...
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bioRxiv - Biophysics 2022Quote: ... we mix into the digestion reaction a trace amount of polystyrene microspheres (Polysciences), of diameter a = 1 μm ...
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bioRxiv - Cell Biology 2022Quote: ... polyplexes were assembled by combining Cy5 labelled PEI (as above) and transfection grade PEI (PEI max linear, Polysciences, MW 40,000) at a weight ratio of 1:5 respectively ...
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bioRxiv - Bioengineering 2022Quote: ... infiltrated in JB4 medium (Polysciences, Warrington, PA) for several weeks and subsequently embedded in JB4 plastic ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... PEI MAX was purchased from Polysciences Inc ...
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bioRxiv - Biophysics 2022Quote: ... for 1 hour and finally mounted on glass slides with Mowiol 4-88 (Polysciences, Inc.) and kept at 4°C overnight.
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bioRxiv - Cancer Biology 2022Quote: ... 10 µl of activated Concanavalin A coated magnetic beads (Polysciences) were added per sample and incubated at room temperature (RT ...
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bioRxiv - Cell Biology 2022Quote: ... embryos were mounted onto glass slides using AquaPolymount (Polysciences).
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were mounted in Aqua-Poly/Mount (Polysciences, Inc) and imaged with a Leica TCS SP8 confocal microscope using 63x ...
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bioRxiv - Microbiology 2022Quote: ... Polystyrene particles (beads) with a diameter of 0.5 μm (Polysciences, USA), were then delivered into the flow-cell and allowed to attach to the filament stubs ...
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bioRxiv - Microbiology 2022Quote: ... 10 μl of a 1 μm polystyrene bead solution (Polysciences, USA), 100 μl QUEEN buffer wash ...
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bioRxiv - Neuroscience 2022Quote: ... Animals were transferred onto 10% agarose pads in M9 buffer and immobilized with Polybead polystyrene 0.1 mm microspheres (Polysciences Inc., Warrington, PA, USA).
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bioRxiv - Immunology 2022Quote: ... were transfected into the HEK293F cells using polyethylenimine (Polysciences). The conditioned media were harvested and concentrated using a Hydrosart ultrafilter (Sartorius) ...
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bioRxiv - Cell Biology 2022Quote: ... and mounted with 20 μm polystyrene beads (Polysciences, Inc.) between a slide and coverslip as in (Rodriguez et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Embryos were collected and mounted in 8-10 μL of egg buffer supplemented with 18.8μm polystyrene beads (Polysciences, Inc.).