Labshake search
Citations for Zymo Research :
6451 - 6500 of 6633 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... pisum LSR1 RNA was extracted from a single aphid using the Zymo Quick RNA Miniprep Kit according to the manufacturer protocols (Zymo Research, CA, USA). The RNA was then reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... followed by (ii) RNA purification and on-column-digestion of genomic DNA using the Direct-zol RNA Miniprep kit (Zymo Research, Freiburg, Germany), and (iii ...
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bioRxiv - Genomics 2022Quote: Up to 75 ng of sheared gDNA was subjected to bisulfite conversion using the EZ-96 DNA Methylation Kit (Zymo Research; Irvine, CA), with liquid handling on a Hamilton MicroLab STAR (Hamilton ...
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bioRxiv - Genetics 2022Quote: ... 1 μg of plasmid DNA was digested with NotI and CIP overnight and then purified with the Zymo DNA Clean and Concentrator kit (Zymo Research cat. D4030). Next ...
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bioRxiv - Genomics 2022Quote: ... Genomic DNA (500□ng) from whole blood was bisulfite treated using the Zymo EZ DNA Methylation kit (Zymo Research Corp, Irvine, CA, USA), and 4□μl of bisulfite- converted DNA was measured on the Illumina 450k array[14] following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... and an aliquot of 500□ng of genomic DNA was treated with sodium bisulfite using the EZ DNA Methylation Kit according to the manufacturer’s instructions (Zymo Research, Irvine, CA, USA). The Infinium Methylation EPIC BeadChip technology (Illumina ...
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bioRxiv - Genomics 2022Quote: ... 750 ng of DNA was extracted from whole blood and bisulfite converted using the EZ DNA Methylation kit (Zymo Research, Irvine, CA, USA). Methylation was measured from 160 ng of bisulfite-converted DNA using the Illumina EPIC Beadchip (Illumina Inc ...
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bioRxiv - Plant Biology 2022Quote: ... including a DNase treatment according to manufacturer’s instructions and was further purified using the RNA Clean & Concentrator Kits (Zymo Research®, California, U.S.A.). RNA-seq libraries were constructed by the POPS platform (IPS2 ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from 50-100 mg seedlings using the Quick-RNA Miniprep Kit with on-column DNase I digestion (Zymo Research, Irvine, CA). cDNA was synthesized with 2–2.5 μg total RNA using the Invitrogen SuperScript III Reverse Transcriptase and the Oligo(dT)20 primer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... was cleaned and concentrated with either sparQ PureMag beads (QuantaBio, Beverly, MA) or the Genomic DNA Clean & Concentrator-10 Kit (Zymo Research, Irvine, CA), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: Yeast cells transformed with plasmids encoding the CDM library or control variant are harvested and the plasmid DNA extracted using the Zymoprep Yeast Plasmid Miniprep I Kit (Zymo Research, Irvine, CA). DNA sequences of the variant library are then PCR-amplified using primers with NGS adapters and gel-extracted from the reaction mixture by length of the PCR product ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cells were then harvested by centrifugation at 3500g for 4 min and prepared for transformation using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA). For plasmid transformations ...
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bioRxiv - Microbiology 2022Quote: ... samples were processed for RNA extraction and purification using the Zymo Direct-zol™ RNA Mini Prep kit (Zymo Research, Irvine, CA, USA) per manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were separated by electrophoresis on a 1% agarose gel and purified using the Zymoclean Gel DNA recovery Kit (Zymo Research, Irvine, CA). The purified DNA fragments were then submitted for Sanger sequencing (GENEWIZ ...
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bioRxiv - Pathology 2022Quote: Approximately 10 mg of each tissue was macerated in extraction buffer and RNA extracted using the ZYMO Research Quick DNA/RNA Pathogen Miniprep kit (ZYMO Research, Irvine, California) according to the manufacturer’s directions ...
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bioRxiv - Systems Biology 2022Quote: ... The renal tubules were washed in dishes containing ice-cold DPBS by pipetting to remove contaminants before RNA extraction using a Direct-zol RNA MicroPrep kit (Zymo Research, Irvine, CA). Four to eight tubules were collected for each sample for a total length of 2 – 4 mm.
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bioRxiv - Plant Biology 2022Quote: ... The sheared DNA was cleaned up and recovered by 1.2x AMPure XP beads followed one round of sodium bisulfite conversion using the EZ DNA Methylation-Lightning Kit (Zymo Research Corporation, Irvine, CA) as outlined in the manufacturer’s instruction with 80 min of conversion time ...
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bioRxiv - Plant Biology 2022Quote: ... Fragments were resolved with gel electrophoresis (1% agarose in 1x TAE buffer) and excised using the Zymoclean Gel DNA Recovery Kit (Zymo Research; Ref.: D4008) for elution of high-concentration ultra-pure DNA ...
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bioRxiv - Plant Biology 2022Quote: ... 2.5 μg of CTAB extracted-DNA per biological replicate were first treated with sodium-bisulfite using the Zymo EZ DNA Methylation-Lightning™ Kit (Zymo Research Corp.) and then built into 150 bp PE read libraries with the TruSeq Nano DNA LT Sample Prep Kit (Illumina Inc. ...
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bioRxiv - Physiology 2022Quote: ... Samples thawed and then disrupted with an eppendorph tube pestle and total RNA purified using a Direct-zol plus RNA miniprep kit (Zymo Research, Irvine, CA) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Two-third of final flag elute was taken for DNase treatment using NEB DNase 1 (M0303) and RNA was purified using RNA Clean and Concentrator Kit (Zymo Research, R1015/R1017). The final RNA concentration was determined using Qubit RNA high Sensitivity Assay Kit and 100ng of RIP-purified RNA was used for cDNA library preparation using NEBNext Ultra II directional RNA library kit (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH5α λpir (Table 1) was prepared using the commercially available Mix and Go™ transformation kit (Zymo Research International, Irvin, CA, USA) according to the manufacturer’s indications ...
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bioRxiv - Neuroscience 2024Quote: ... Larval and adult samples were completely homogenized in RNA Lysis buffer from the Quick-RNA miniprep Kit (R1055, Zymo Research, Irvine, CA, USA) using a pestle and micro-tube homogenizer (for 30 sec ...
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bioRxiv - Developmental Biology 2024Quote: ... digested with RNase and Proteinase K and the DNA cleaned and concentrated using ChIP DNA cleanup and concentrator kit (Zymo research Catalog# D5201) according to manufacturer instructions ...
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bioRxiv - Zoology 2024Quote: ... and the supernatant was collected into a new tube where RNA was extracted using the Direct-zol RNA Miniprep kit (Zymo cat. no. R2050) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: For long read sequencing DNA extractions of Staphylococcus aureus ST188 and selected evolved isolates were performed using the Quick-DNA™ High Molecular Weight (HMW) MagBead Kit (Zymo Research, UK) with the addition of 100 µL of 0.5 mg/mL lysostaphin (from Staphylococcus staphylolyticus ...
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bioRxiv - Evolutionary Biology 2024Quote: ... High yield pure plasmid DNA preparations were subsequently obtained from 100 mL overnight LB broth cultures using the endo-free ZymoPURE™ II Plasmid Midiprep Kit (Zymo Research, USA).
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bioRxiv - Genetics 2024Quote: ... 2016) Genomic DNA (500ng) from whole blood underwent bisulfite treatment with the Zymo EZ DNA Methylation kit (Zymo Research Corp, Irvine, CA, USA), and 4 µl of bisulfite-converted DNA was measured on the Illumina 450k array following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 6-500 ng of DNA from each sample was bisulfite-converted using the Zymo EZ DNA Methylation Kit (Zymo Research, Irvine, CA USA) following the manufacturer’s protocol and the specified modifications for the Illumina Infinium methylation assays ...
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bioRxiv - Genetics 2024Quote: ... Five hundred nanograms of DNA per sample were bisulfite-treated using the Zymo EZ-96 DNA methylation kit (Zymo Research, Orange, CA, USA). A bisulfite sequencing PCR approach was applied to the promoters of 12 candidate genes (Supplementary Table S3) ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from the solid and liquid aliquots using the Qiagen AllPrep PowerViral DNA/RNA kit and further purified using the Zymo OneStep PCR inhibitor removal columns (Zymo Research, Irvine, CA) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... genomic DNA was extracted from 2-10 mL of stationary cultures with the Zymo Research Midiprep gDNA kit (Zymo Research EUROPE GmbH, Germany). Library preparation and sequencing were performed at Novogene (UK ...
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bioRxiv - Microbiology 2023Quote: ... and DNA extraction from the 2017 and 2018 (30 May and 26 July) samples were performed using the ZymoBIOMICS™ DNA MiniPrep kit (Zymo Research Corp.). Simultaneous DNA and RNA extractions were performed on 29 September 2018 ...
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bioRxiv - Genomics 2023Quote: ... Total RNA of the parents and two bulks was isolated using the Direct-ZolTM RNA Kits (Zymo Research Co., Ltd., Irvine, CA, USA). RNA quality was determined using a NanoDrop OneC spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Purification was performed according to manufacturer’s instructions and the converted DNA was then eluted in 32.5 µl nuclease-free water (17 µl × 2 times) through a column from the DNA Clean & Concentrator kit (Zymo Research, cat. no. D4003).
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bioRxiv - Microbiology 2023Quote: ... Remaining material from the same DNA extract of subculture 19.9 was then concentrated using the DNA Clean and Concentrator-5 kit (Zymo Research; Irvine, California, U.S.A.) and used for long read sequencing ...
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bioRxiv - Bioengineering 2023Quote: ... Undigested bands corresponding to mutant PCR products were gel extracted with the Zymoclean Gel DNA Recovery Kit (Zymo Research, Cat. No./ID: D4007), then cloned into pJET vectors (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μL of each reaction product were pooled and purified using the Zymo DNA Clean and Concentrator kit (Zymo Research, Irvine, CA). Sequencing was performed at the Chan Zuckerberg BioHub facility on an Illumina NextSeq 550 platform in High Output mode.
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bioRxiv - Developmental Biology 2023Quote: ... and the aqueous phase was carefully transferred into new tubes and processed with the Direct-zol RNA Purification Kit (Zymo Research, cat. #R2051) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Five-hundred nanograms of DNA per sample underwent bisulfite conversion using the EZ-96 DNA Methylation kit (Shallow) (Zymo Research Corporation, Irvine, USA) and further processed with the Illumina Infinium HumanMethylation450 BeadChip (Illumina Inc. ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Total RNA was isolated from polarized murine bone marrow-derived macrophages using the Quick-RNA™ MiniPrep Kit (Zymo Research, Irvine, CA, USA). RNA quantity and quality was verified by two methods ...
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bioRxiv - Bioengineering 2023Quote: ... and total genomic DNA was extracted from algal cell pellets (∼50-100 mg) with a Zymo Quick-DNA fungal/bacterial extraction kit (Zymo Research group, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... was transformed with 2 μL of each ligation reaction and resulting colonies were selected for plasmid DNA isolation using the ZymoPure Plasmid miniprep kit (Zymo Research, Cat. #D4209). Each cloned gRNA sequence-containing PB- CRISPRia plasmid was verified by Sanger sequencing (Quintara Bio) ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from primary cardiac fibroblasts or primary lung fibroblasts from C57BL/6J mice using an RNA isolation kit (Zymo, cat. no. R1018) for RT-qPCR ...
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bioRxiv - Cell Biology 2023Quote: ... This integrated the DNA denaturation and bisulfite conversion processes into one-step by the EZ DNA Methylation-Gold™ Kit (ZYMO Research, USA). The eluted DNA sample was stored at 20°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The outer PCR products amplified using primer #1 and #2 were purified and concentrated using the DNA Clean and Concentrator 5 kit (Zymo Research, Orange, CA) and eluted in 20ul of TE buffer ...
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bioRxiv - Genomics 2022Quote: RNA was extracted from the nine individual tissue samples stored in RNAlater™ using the Direct-zol™ RNA Miniprep Plus kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... RNA from each individual wasp after chemical extraction (see below) was obtained using the Quick-RNA Tissue/Insect Kit (Zymo Research, Freiburg, Germany), and reversely transcribed into complemetary DNA (cDNA ...
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bioRxiv - Biochemistry 2023Quote: ... 1 volume of ethanol was added to the aqueous layer and the mixture was applied to and eluted from a Direct-zol column (Zymo RNA miniprep kit) yielding 25 µg of RNA.
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bioRxiv - Cancer Biology 2023Quote: ... We extracted gDNA and RNA from the tissue blocks simultaneously using the Quick-DNA/RNA Microprep Plus Kit (ZYMO Research, cat. no. D7005). We first homogenize the tissues that were stored in 1XDNA/RNA Shield following the instructions for Tough-to-Lyse samples using Biomedical FastPrep Homogenizer (MP Bio) ...