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Citations for Akoya Biosciences :
351 - 400 of 1480 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... the slides were incubated with Opal 520 reagent (1:200, FP1488001KT, Akoya Biosciences) for 15 min ...
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bioRxiv - Pathology 2024Quote: ... slides were incubated with Opal fluorophore reagents (Akoya Biosciences, Marlborough, MA, US). Sections with the fibulin-1 antibody were incubated with Opal 650 reagent (1:200 ...
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bioRxiv - Pathology 2024Quote: ... slides were incubated with Opal 570 reagent (1:200, FP1488001KT, Akoya Biosciences) for 15 min ...
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bioRxiv - Pathology 2024Quote: ... Sections with the fibulin-1 antibody were incubated with Opal 650 reagent (1:200, FP1496001KT, Akoya Biosciences) diluted in 100mM borate buffer pH 8.5 containing 0.1% Tween-20 ...
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bioRxiv - Developmental Biology 2024Quote: ... OpalTM 570 (FP1488001KT, Akoya Biosciences) and OpalTM 690 (FP1497001KT ...
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bioRxiv - Developmental Biology 2024Quote: ... and OpalTM 690 (FP1497001KT, Akoya Biosciences) fluorophores were used.
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bioRxiv - Neuroscience 2024Quote: ... and the RNAscope LS multiplex fluorescent reagent kit (Advanced Cell Diagnostics, Bio-Techne) with Opal fluorophore reagent pack detection (Akoya BioSciences, Inc.). All slides were counterstained with DAPI and coverslipped with ProLong Diamond antifade mountant (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... and opal dyes (520 nm for channel 1; 570 nm for channel 2; 690 nm for channel 3; Akoya Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... and Opal™ 570 (Akoya Biosciences, #FP1488001KT). Immunohistochemistry Part B was then performed by incubation with ...
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bioRxiv - Neuroscience 2024Quote: ... A series of 3 amplification steps were performed along with the development of fluorescent signal using Opal™ 520 (Akoya Biosciences, #FP1487001KT), and Opal™ 570 (Akoya Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: ... Tyramide signal amplification (TSA) fluorophores (Akoya Bioscience ...
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bioRxiv - Neuroscience 2024Quote: ... antibodies to evaluate the co-expression of ELANE with microglia or neutrophil using an Opal 3-Plex Manual Detection kit (# NEL810001KT, Akoya Bioscience, Marlborough, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Acquired images were spectrally unmixed and image export was performed using the built-in synthetic spectral library of the inForm software (Akoya Biosciences). For the SPG panel ...
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bioRxiv - Molecular Biology 2024Quote: ... the scanned slides were batch exported using the Akoya Phenochart software (Akoya Biosciences) and using a synthetic library or panel-specific library ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were scanned using PhenoImager (Akoya Biosciences) according to the manufac-turer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 690 (Cat#OP-001006) from Akoya Biosciences (Marlborough ...
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bioRxiv - Molecular Biology 2024Quote: ... Fluorescent dyes Opal 690 (FP1497001KT) and Opal 620 (FP1495001KT) (Akoya Biosciences, Marlborough, MA) were used with each of these probes ...
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bioRxiv - Microbiology 2024Quote: ... and 1:750 dilution of OPAL 570 (AKOYA, Biosciences®). Immunohistochemistry for HHV8 LANA protein was performed after RNAScope using a mouse anti-human HHV8_LNA ...
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bioRxiv - Neuroscience 2024Quote: ... TSA Plus Fluorescein Systems from PerkinElmer (TSA plus Cyanine detection kit, Akoya) was used according to the manufacturer’s protocol for DIG detection ...
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bioRxiv - Immunology 2024Quote: ... Slides were imaged with PhenoImager™ HT instrument (Akoya Biosciences). Affected lung area was quantified in relation to the whole lung area and the number of the different cell subsets in relation to all cells using the software QuPath (freeware).
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bioRxiv - Immunology 2024Quote: ... Single stain and auto fluorescence (AF) controls were used for creating an acquisition protocol in InForm software (Akoya Biosciences).
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bioRxiv - Neuroscience 2024Quote: ... The fluorophores applied to detect the signal from the probes were Opal 690 Reagent (1:750; Akoya Biosciences, #FP1497001KT) or Opal 570 Reagent (1:750 ...
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bioRxiv - Neuroscience 2024Quote: ... or Opal 570 Reagent (1:750; Akoya Biosciences, #FP1488001KT). Slides were rinsed with PBS-tween ...
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bioRxiv - Immunology 2024Quote: ... Placental sections were dehydrated and enveloped in permanent mounting medium (VectaMount H-5000) before scanning using the PhenoImager (Akoya Biosciences).
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bioRxiv - Neuroscience 2024Quote: ... Fluorescein conjugated TSA reagent (Akoya Biosciences, NEL745001KT) or Cy3 reagent (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then imaged at 40X using the PhenoImager HT 2.0 (Akoya Biosciences) at 40X ...
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bioRxiv - Cancer Biology 2024Quote: ... and DAPI channels on a Vectra 3 whole slide scanning microscope (Akoya Biosciences), viewed using Phenochart software ...
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bioRxiv - Immunology 2024Quote: ... Slides were counterstained with spectral DAPI (Akoya Biosciences, FP1490) and mounted with ProLong Gold Antifade (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... Whole slide was scanned at 20x in the motif mode and the region of interest was selected for spectral unmixing and image processing using InForm v.2.4.10 software from Akoya Biosciences ...
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bioRxiv - Immunology 2024Quote: ... and processed with CODEX Processor v1.7 (Akoya Biosciences).
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bioRxiv - Immunology 2024Quote: ... Raw files were exported using the CODEX Instrument Manager (Akoya Biosciences, Marlborough, MA, USA) and processed with CODEX Processor v1.7 (Akoya Biosciences).
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bioRxiv - Immunology 2024Quote: ... A multicycle CODEX experiment was performed following manufacturer’s instructions (Akoya Biosciences). Images were acquired with a Zeiss Axio Observer widefield fluorescence microscope using a 20x objective (NA 0.85) ...
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bioRxiv - Immunology 2024Quote: Images were analyzed in the CODEX MAV software (Akoya Biosciences). First ...
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bioRxiv - Immunology 2024Quote: ... For immunofluorescence staining we followed the Opal IHC assay guide (Akoya Biosciences) and used the following primary antibodies ...
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bioRxiv - Immunology 2024Quote: ... Signal amplification was achieved using TSA working solution diluted at 1:150 in 1 × amplification diluent (Akoya Biosciences) and incubated at room temperature for 10 min ...
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bioRxiv - Immunology 2024Quote: ... A secondary HRP antibody (Akoya Biosciences) was added and incubated at room temperature for 10 min ...
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bioRxiv - Immunology 2024Quote: ... The staining for mIHC was carried out with the Opal Polaris 7 color IHC Detecton Kit (Akoya Biosciences, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... A microwave treatment in the AR6 buffer (Akoya Biosciences) was applied for antigen retrieval ...
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bioRxiv - Immunology 2024Quote: ... The multispectral imaging was collected by Vectra Polaris™ Automated Quantitative Pathology Imaging System (Akoya Biosciences) at 20× magnification and analyzed by HALO software (indica labs).
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bioRxiv - Bioengineering 2024Quote: Multiplex immunofluorescence staining was performed using the Opal 4-Color IHC Kit (Akoya Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... protein staining and image acquisition was performed on a PhenoCycler-Fusion system (Akoya Biosciences, Marlborough, MA, USA) according to manufacturer instructions before HE staining.
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bioRxiv - Immunology 2024Quote: ... RNAscope multiplex fluorescent kit V2 (Advanced Cell Diagnostics, UM 323100) with the opal fluorophores system (Akoya Bioscience) were used to simultaneously detect HIV vRNA+ and transduced CAR/CXCR5 cells according to the manufacturer’s instructions and as described previously (10 ...
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bioRxiv - Immunology 2024Quote: ... the PhenoImager™ HT (Akoya Biosciences) system at Birmingham Tissue Analytics was used ...
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bioRxiv - Immunology 2024Quote: ... version 2.4.8 (Akoya). Initially ...
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bioRxiv - Immunology 2024Quote: ... The detection of the target proteins was achieved using optimized fluorescent Opal tyramide signal amplification (TSA) dyes (Akoya), along with repeated cycles of antibody denaturation ...
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bioRxiv - Immunology 2024Quote: ... Multispectral images (MSI) were acquired using i) the Vectra Polaris 1.0 imaging system (Akoya) at a resolution of 5µm/pixel (20x ...
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bioRxiv - Immunology 2024Quote: Multiparametric immunofluorescence staining based on tyramide signal amplification (OPAL, Akoya, #OP7DS2001KT) was used to stain TAB sections with a set of different markers ...
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bioRxiv - Immunology 2024Quote: ... incubated with blocking antibody diluent (Akoya, ARD1001EA) for 10 minutes ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The fluorescence signal was developed using the fluorophore-conjugated tyramide technology using TSA Plus Cyanine 3 and 5 kits (Akoya Biosciences). Embryos were mounted and imaged using a Zeiss LSM 700 confocal microscope.
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bioRxiv - Neuroscience 2024Quote: ... Cyanine 3-conjugated tyramide (1:150, Akoya) was applied in the dark for 5-6 minutes ...