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Citations for Akoya Biosciences :
301 - 350 of 1480 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Kir6.1, a component of an ATP-sensitive potassium channel, regulates natural killer cell developmentbioRxiv - Immunology 2024Quote: ... Opal dyes (Akoya Biosciences, MA) were used for secondary staining as follows ...
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bioRxiv - Immunology 2024Quote: ... Endogenous peroxidase activity was blocked using Antibody Diluent/Block (Akoya Bioscience, US) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: The slides were visualized using the Vectra Polaris system (Akoya Biosciences, US), and a multispectral image of the whole slide was scanned using a 20× objective lens ...
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bioRxiv - Immunology 2024Quote: ... Slides were scanned at 20× magnification using the Vectra Polaris system (Akoya Biosciences, US). Whole-tissue scans were analyzed with Qupath software (https://qupath.github.io ...
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bioRxiv - Immunology 2024Quote: ... Nuclear staining was performed using DAPI (Akoya Bioscience, US) for 5 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: Multiplex IHC staining was performed using an Opal 7-color kit (Akoya Bioscience, US). Briefly ...
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bioRxiv - Immunology 2024Quote: ... the Phenochart 1.0.12 software (Akoya), a whole-slide viewer for high-resolution multispectral acquisition and annotation capabilities ...
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bioRxiv - Immunology 2024Quote: ... incubated with Opal Polymer HRP (anti-mouse and anti-rabbit, Akoya, ARH1001EA) for 10 minutes and labeled with Opal dyes (Akoya ...
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bioRxiv - Immunology 2024Quote: ... for 10 minutes and labeled with Opal dyes (Akoya, OP-01000 ...
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bioRxiv - Immunology 2024Quote: ... and images were acquired by Vectra Polaris PhenoImager HT 2.0 slide scanner and software (Akoya Biosciences). Two male and one female mice from two from two separate experiments were sectioned and quantified ...
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bioRxiv - Developmental Biology 2024Quote: ... Fluorescence was revealed with the TSA+ Cyanin 3 or 5 system (AKOYA biosciences).
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bioRxiv - Evolutionary Biology 2024Quote: ... Prolactin signal was amplified with TSA-Cy3 (Akoya Biosciences NEL744001KT) for 6m (RT ...
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bioRxiv - Neuroscience 2024Quote: ... the FITC-labeled probe was visualized using tyramide-FITC Plus (1/50 dilution, 15-minute incubation, Akoya Biosciences). Following washes in TNT ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed and incubated with TSA solution (#NEL744001KT, Akoya Biosciences) overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... After the described washes and blocking steps the DIG-labeled probes were visualized using tyramide-Cy3 Plus (1/75 dilution, 15-minute incubation, Akoya Biosciences). After washes in TNT ...
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bioRxiv - Neuroscience 2024Quote: ... probes were amplified and fluorescently labeled with Opal dyes as follows: PROX1 was labeled with 1:500 Opal 520 (catalog no. FP1487001KT, Akoya Biosciences [AB]), LAMP5 was labeled with 1:500 Opal 690 (catalog no ...
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bioRxiv - Neuroscience 2024Quote: ... The signal was developed using the HRP for each channel and then opal dyes diluted at 1:1000 were assigned for each channel: tdTomato was assigned to Opal 690 (Akoya Bioscience, catalog # FP1497001KT), Glun2c was assigned to Opal 570 (Akoya Bioscience ...
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bioRxiv - Neuroscience 2024Quote: ... and Glun2d was assigned to Opal 520 (Akoya Bioscience, catalog # FP1487001KT). Slices were counterstained with DAPI and coverslipped with ProLong Gold antifade mountant (Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: Slides stained with mIF panels IP1.2 and IP2.2 were scanned using the Vectra Polaris multispectral imaging system (Akoya Biosciences). Regions of interest were selected in Phenochart (Akoya ...
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bioRxiv - Developmental Biology 2024Quote: ... a different blocking buffer was used (for mouse and rabbit primary Ab: Akoya Biosciences ...
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bioRxiv - Developmental Biology 2024Quote: ... For the multiplex mIHC we used the Opal 6 plex detection kit (Akoya Biosciences, NEL871001KT) and staining was conducted according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Whole slide images were collected at 10x magnification using the PhenoImager multispectral slide scanner (V1.0.13, Akoya Biosciences). A TMA map was applied using Phenochart (v1.1.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... A TMA map was applied using Phenochart (v1.1.0, Akoya Biosciences) and individual core images acquired at 20x magnification ...
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bioRxiv - Cancer Biology 2024Quote: FFPE tissue sections (4µm) were processed manually for mIHC using Opal reagents (Akoya Biosciences) for illustration purposes in four representative samples (2 in the No-NAT and TNT groups) ...
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bioRxiv - Cancer Biology 2024Quote: ... and unmixed using InForm 4.8 (Akoya Biosciences) software.
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bioRxiv - Neuroscience 2024Quote: ... Detection of mRNA was achieved using Opal™ fluorophore dyes from the 520 (1:500) and 620 (1:750) reagent packs (Akoya Biosciences, USA). Full protocol details are available in Supplementary Methods ...
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bioRxiv - Cancer Biology 2024Quote: ... and spectrally unmixed using inForm software (Akoya). Unmixed image tiles were then stitched together and cell segmentation performed in QuPath44 ...
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bioRxiv - Cancer Biology 2024Quote: ... Regions of interest were selected in Phenochart (Akoya) and spectrally unmixed using inForm software (Akoya) ...
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bioRxiv - Cancer Biology 2024Quote: Multiplex immunofluorescence (mIF) analysis was performed with tyramide signal amplification using Opal fluorescent dyes (Akoya Biosciences). Three mIF panels were used in this study (IP1.2 ...
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bioRxiv - Developmental Biology 2024Quote: ... slides were incubated for 30 minutes at RT with primary Ab which was diluted in TNB buffer (Akoya Biosciences, FP1020), followed by incubation with secondary antibodies ImmPRESS-HRP anti mouse/rabbit (Akoya Biosciences ...
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bioRxiv - Developmental Biology 2024Quote: ... nuclei were counterstained with DAPI (Akoya Biosciences, FP1490) for 5 minutes at RT before mounting the stained tissue with mounting medium (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... a different blocking buffer was used (for mouse and rabbit primary Ab: Akoya Biosciences, ARD1001EA; for rat primary Ab: Akoya Bioscience, 30024). Next ...
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bioRxiv - Developmental Biology 2024Quote: ... Stained sections were imaged using a slide scanner (Akoya vector Polaris).
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bioRxiv - Developmental Biology 2024Quote: ... All Opal reagents were diluted 1:150 in an amplification buffer (Akoya Biosciences, FP1609) except Opal 780 ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by incubation with secondary antibodies ImmPRESS-HRP anti mouse/rabbit (Akoya Biosciences, ARH1001EA) or ImmPRESS-anti rat HRP (Akoya Biosciences ...
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bioRxiv - Developmental Biology 2024Quote: ... an Opal fluorophore working solution (Akoya Biosciences, NEL871001KT) was applied for 10 minutes at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... or ImmPRESS-anti rat HRP (Akoya Biosciences, 30032) for 10 minutes at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... a tyramide fluorescent substrate (OpalTM520, Akoya Biosciences) was used to incubate the embryos ...
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bioRxiv - Cancer Biology 2024Quote: ... Heat-induced antigen retrieval was achieved in Antigen Retrieval (AR) 9 buffer (Akoya Biosciences) using a microwave (Panasonic with Inverter technology) ...
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bioRxiv - Neuroscience 2024Quote: ... Glun2c was assigned to Opal 570 (Akoya Bioscience, catalog # FP1488001KT), and Glun2d was assigned to Opal 520 (Akoya Bioscience ...
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bioRxiv - Neuroscience 2024Quote: ... Advanced Cell Diagnostics) and labeled using 1:1500 diluted Opal 570 and 690 dyes respectively (Akoya Biosciences). Labeled sections were stained with DAPI ...
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bioRxiv - Neuroscience 2024Quote: ... were applied and amplified in sequence with TSA Vivid and Opal Polaris dyes (Advanced Cell Diagnostics, cat# 323271, 323272, 323273; Akoya Biosciences, cat# FP1501001KT). Cellular nuclei were counterstained with 1 µg/ml DAPI and mounted with Prolong Glass Antifade Mountant (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... or Cy3 reagent (Akoya Biosciences, NEL744001KT) were diluted as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Amplification steps were performed as instructed by manufacturer and signal was developed using HRP-C1 followed by incubation with Opal 570 (Akoya FP1488001KT) with the following dilutions ...
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bioRxiv - Neuroscience 2024Quote: ... the Opal™ 620 Reagent Pack (FP1495001KT, Akoya Biosciences Inc) for 30 min ...
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bioRxiv - Pathology 2024Quote: Slides were incubated with primary antibodies (Table 2) diluted in 1X Plus Automation Amplification Diluent (FP1609, Akoya Biosciences) in the following sequential order ...
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bioRxiv - Pathology 2024Quote: ... the respective Opal dye (Opal Polaris 480 reagent pack, FP1501001KT, Akoya Biosciences ...
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bioRxiv - Pathology 2024Quote: ... Multiplex IF stainings using the Opal dyes from Akoya were digitized with multispectral imaging by the Vectra® Polaris™ (PerkinElmer ...
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bioRxiv - Pathology 2024Quote: The acquired images were saved in TIFF format using Phenochart (Akoya Biosciences). Image processing was performed using the “Color Deconvolution” plugin in Fiji software (H DAB for αSMA IHC) ...
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bioRxiv - Pathology 2024Quote: ... Images were acquired using Vectra Polaris (Akoya Biosciences).