Labshake search
Citations for Akoya Biosciences :
451 - 500 of 1480 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... 690 and 780 (Akoya Biosciences) and with the spectral DAPI (Akoya Biosciences ...
-
bioRxiv - Cancer Biology 2024Quote: ... The spectral unmixing of the images was performed with InForm 2.6 Image Analysis Software (Akoya Biosciences) and the analysis of cell density with QuPath v0.5.1 ...
-
bioRxiv - Cancer Biology 2024Quote: ... and imaged using a Mantra2 quantitative pathology workstation (Akoya Biosciences) at X20 magnification ...
-
bioRxiv - Cell Biology 2024Quote: ... was hybridized following manufacturer instructions using RNAscope Multiplex fluorescent v2 assay (ACD; #323110) followed by detection with TSA plus Fluorescein (Akoya) fluorophore ...
-
bioRxiv - Cancer Biology 2024Quote: ... Markers were prepared in DAKO antibody diluent followed by the polymeric HRP-conjugated secondary antibody (DS9800) and opal fluorophore-conjugated TSA (Akoya Bioscience) at 1:100 dilution before dispensing to the slide sequentially ...
-
bioRxiv - Biochemistry 2024Quote: ... and then incubated for 20-30 min in staining buffer (Akoya). Sections were then covered with 200 µL of antibody buffer composed of staining buffer supplemented with recommended concentrations of N ...
-
bioRxiv - Biochemistry 2024Quote: ... samples were placed in a storage buffer (Akoya Biosciences) and photobleached by illumination with a 200 mA ...
-
bioRxiv - Biochemistry 2024Quote: ... lung tissue was washed with hydration buffer (Akoya Biosciences) and then incubated for 20-30 min in staining buffer (Akoya) ...
-
bioRxiv - Biochemistry 2024Quote: ... & S blockers (Akoya Biosciences) and the specified dilutions of up to 34 antibody-barcode conjugates followed by incubation in a humidified chamber at room temperature for 3 h ...
-
bioRxiv - Cancer Biology 2024Quote: ... and denaturing steps were executed using the PhenoCycler-Fusion instrument from Akoya Biosciences ...
-
bioRxiv - Cancer Biology 2024Quote: ... and phenotyping using inForm software v3.0 (Akoya Biosciences). Exported data were compiled and summarized using PhenoptrReports (Akoya Biosciences) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Exported data were compiled and summarized using PhenoptrReports (Akoya Biosciences). Full antibody details are given in Supplementary Table ST6.
-
bioRxiv - Cancer Biology 2024Quote: A CODEX microfluidic instrument also known as Phenocycler Fusion (Akoya Biosciences Inc., CA, USA) integrated with an inverted fluorescence microscope through a custom stage insert was used to automate CODEX buffer exchange and image acquisition ...
-
bioRxiv - Cancer Biology 2024Quote: ... OPAL620 (AKOYA Biosciences Cat#FP1495001KT), OPAL650 (AKOYA Biosciences Cat#FP1496001KT ...
-
bioRxiv - Cancer Biology 2024Quote: ... OPAL650 (AKOYA Biosciences Cat#FP1496001KT) reagents with Tyramide Signal Amplification ...
-
bioRxiv - Cancer Biology 2024Quote: ... OPAL570 (AKOYA Biosciences Cat#FP1488001KT), OPAL620 (AKOYA Biosciences Cat#FP1495001KT) ...
-
bioRxiv - Cancer Biology 2024Quote: ... secondary antibodies were coupled to OPAL520 (AKOYA Biosciences Cat# FP1487001KT), OPAL570 (AKOYA Biosciences Cat#FP1488001KT) ...
-
bioRxiv - Cancer Biology 2024Quote: ... The images were acquired with Axioscan (Zeiss) (Akoya Biosciences, USA) and analyzed using open-source software QuPath.
-
bioRxiv - Cancer Biology 2024Quote: ... Identified antibody clones were then conjugated with oligonucleotide barcodes using Antibody Conjugation Kit (Akoya Biosciences). Prior to CODEX imaging ...
-
bioRxiv - Cancer Biology 2024Quote: ... fluorescent oligonucleotide-conjugated reporters were combined with Nuclear Stain and CODEX Assay Reagent (Akoya Biosciences) in sealed light-protected 96-well plates (Akoya Biosciences) ...
-
bioRxiv - Cancer Biology 2024Quote: ... InForm (Akoya Biosystems, Marlborough, USA) was used to carry out spectral unmixing and to eliminate autofluorescence from the images.
-
bioRxiv - Cancer Biology 2024Quote: Multiplex IHC was performed using an Opal 7-Color IHC Kit (Akoya Biosciences) on a Bond RX automated stainer (Leica Biosystems) ...
-
bioRxiv - Cell Biology 2024Quote: ... Each section was dewaxed and underwent heat-induced epitope retrieval (HIER) treatment using pH 9 EDTA buffer (Akoya Biosciences, AR900250ML) in a pressure cooker (Bio SB TintoRetriever ...
-
bioRxiv - Cell Biology 2024Quote: ... following a blocking step using TNB buffer containing 0.1 M Tris-HCl /0.15 M NaCl/0.5% blocking reagent pH 7.5 (Akoya, SKU FP1020) for 30 min ...
-
bioRxiv - Bioengineering 2024Quote: ... Multi-plex staining was performed according to the manufacturer’s specifications (Akoya Biosciences). Slides were imaged on an Akoya Phenocycler.
-
bioRxiv - Cancer Biology 2024Quote: ... and stained with using CODEX Staining Kit (Akoya Biosciences). A cocktail of above-conjugated antibodies were incubated with tissue overnight at 4°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... Automated fluidics exchange and image acquisition were performed using the Akoya CODEX instrument integrated with a BZ-X810 epifluorescence microscope (Keyence) and CODEX Instrument Manager (CIM) v1.30 software (Akoya Biosciences). The exposure times were as follows ...
-
bioRxiv - Cancer Biology 2024Quote: ... and background subtraction were performed using the CODEX processor v1.7 (Akoya Biosciences) per manufacture instruction (https://help.codex.bio/codex/processor/technical-notes) ...
-
bioRxiv - Cancer Biology 2024Quote: ... in sealed light-protected 96-well plates (Akoya Biosciences). Automated fluidics exchange and image acquisition were performed using the Akoya CODEX instrument integrated with a BZ-X810 epifluorescence microscope (Keyence ...
-
bioRxiv - Cancer Biology 2024Quote: ... Five images were acquired for each tumor and images were processed using the Inform® 2.5 software from Akoya Biosciences® ...
-
bioRxiv - Cancer Biology 2024Quote: ... and with the spectral DAPI (Akoya Biosciences) as a nuclear counterstain ...
-
bioRxiv - Cancer Biology 2024Quote: The slides were then incubated for 10 minutes with the horseradish peroxidase-conjugated secondary antibodies Opal polymer HRP mouse-rabbit (Akoya Biosciences), ImmPRESS® HRP Goat Anti-Rat IgG HRP-polymer or ImmPRESS® HRP Goat Anti-Rabbit IgG HRP-polymer (Vector Laboratories) ...
-
bioRxiv - Cancer Biology 2024Quote: The staining of 4 µm-thick formalin-fixed paraffin-embedded (FFPE) tissue sections for mIHC analysis was carried out using the tyramide signal amplification-based Opal method (Akoya Biosciences) on a Leica BOND RX automated immunostainer (Leica Microsystems) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Images of tissue sections were acquired with the automated Vectra Polaris system (Akoya Biosciences, USA) and analyzed with the open-source software FIJI.
-
bioRxiv - Cancer Biology 2024Quote: ... The images were acquired with Vectra Polaris automated system (Akoya Biosciences, USA) and analyzed using open-source software QuPath.
-
bioRxiv - Cancer Biology 2024Quote: ... and multispectral immunofluorescence imaging (Vectra, Akoya Biosciences, Marlborough, MA, USA) data which were then further analysed at the Institute of Bioinformatics of the Medical University of Innsbruck ...
-
bioRxiv - Cancer Biology 2024Quote: ... and then scanned with the Vectra® 3 (Akoya Biosystems ...
-
bioRxiv - Cell Biology 2024Quote: ... Fixed islets were subsequently hybridized with custom-designed Mus musculus Cplx 2 (Mm-Cplx 2-C3) probe and developed with Opal-520 (FP1487001KT; 1:1000; Akoya Biosciences). Fixed islets were then immunostained with primary antibodies against either insulin (2D11-H5 ...
-
bioRxiv - Cancer Biology 2024Quote: ... For every cycle we blocked for non-specific binding for 10 minutes in primary diluent (Akoya), incubated the primary antibody for 1 hour ...
-
bioRxiv - Cancer Biology 2024Quote: ... the best focal plane of vertical image stacks collected at each acquisition were chosen for cell segmentation using a gradient-tracing watershed algorithm (Akoya built-in software) on the nuclear staining (radius set to 6 pixels) ...
-
bioRxiv - Cancer Biology 2024Quote: ... slides were stained on a Leica BOND Rx autostainer using the Akoya Opal Multiplex IHC assay (Akoya Biosciences). All primary antibodies were incubated for 1 hour at room temperature ...
-
bioRxiv - Cancer Biology 2024Quote: ... Spectral deconvolution was then performed using inForm 2.4.4 (Akoya Biosciences). High-resolution multispectral images were then fused and analyzed using the software HALO (Indica Labs) ...
-
bioRxiv - Cancer Biology 2024Quote: ... staining was performed with the Opal Kit for multiplex IHC (Akoya Bioscience). Briefly ...
-
bioRxiv - Cancer Biology 2024Quote: ... The Vectra Polaris Automated Quantitative Pathology Imaging System (Akoya Biosciences) was used for multispectral imaging at 20× magnification ...
-
bioRxiv - Cancer Biology 2024Quote: ... all the slide images were loaded into inForm® Tissue Analysis Software (Version 2.6.0, Akoya Bioscience) to perform batch processing and image analysis for cell density profiles ...
-
bioRxiv - Cell Biology 2024Quote: ... and all C3 probes with TSA-Opal 520 (all TSA dyes supplied by Akoya biosciences). Nuclear labelling used Hoechst 33342 (1:2000 dilution ...
-
bioRxiv - Cancer Biology 2024Quote: ... antigen retrieved (AR6 solution, Akoya Bioscience) and blocked with 10% human serum (ThermoFisher) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Slides were mounted and imaged with the PhenoImager HT instrument (Akoya Biosciences). Due to the variable staining CD4 stain across the patient dataset ...
-
bioRxiv - Cancer Biology 2024Quote: ... cell segmentation and positive channel marker thresholding were undertaken using the InForm (version 2.6 AKOYA BioSciences) program ...
-
bioRxiv - Cancer Biology 2024Quote: ... Slides were stained for markers using the 7-plex Opal Polaris kit (Akoya Biosciences) according to the manufacturer’s instructions ...