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Citations for Akoya Biosciences :
151 - 200 of 1480 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... For the multiplex mIHC we used the Opal 6 plex detection kit (Akoya Biosciences, NEL871001KT) and staining was conducted according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Glun2c was assigned to Opal 570 (Akoya Bioscience, catalog # FP1488001KT), and Glun2d was assigned to Opal 520 (Akoya Bioscience ...
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bioRxiv - Neuroscience 2024Quote: ... the FITC-labeled probe was visualized using tyramide-FITC Plus (1/50 dilution, 15-minute incubation, Akoya Biosciences). Following washes in TNT ...
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bioRxiv - Neuroscience 2024Quote: ... The signal was developed using the HRP for each channel and then opal dyes diluted at 1:1000 were assigned for each channel: tdTomato was assigned to Opal 690 (Akoya Bioscience, catalog # FP1497001KT), Glun2c was assigned to Opal 570 (Akoya Bioscience ...
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bioRxiv - Neuroscience 2024Quote: ... and Glun2d was assigned to Opal 520 (Akoya Bioscience, catalog # FP1487001KT). Slices were counterstained with DAPI and coverslipped with ProLong Gold antifade mountant (Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed and incubated with TSA solution (#NEL744001KT, Akoya Biosciences) overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... After the described washes and blocking steps the DIG-labeled probes were visualized using tyramide-Cy3 Plus (1/75 dilution, 15-minute incubation, Akoya Biosciences). After washes in TNT ...
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bioRxiv - Neuroscience 2024Quote: ... probes were amplified and fluorescently labeled with Opal dyes as follows: PROX1 was labeled with 1:500 Opal 520 (catalog no. FP1487001KT, Akoya Biosciences [AB]), LAMP5 was labeled with 1:500 Opal 690 (catalog no ...
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bioRxiv - Cancer Biology 2024Quote: ... and spectrally unmixed using inForm software (Akoya). Unmixed image tiles were then stitched together and cell segmentation performed in QuPath44 ...
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bioRxiv - Cancer Biology 2024Quote: ... Regions of interest were selected in Phenochart (Akoya) and spectrally unmixed using inForm software (Akoya) ...
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bioRxiv - Cancer Biology 2024Quote: Multiplex immunofluorescence (mIF) analysis was performed with tyramide signal amplification using Opal fluorescent dyes (Akoya Biosciences). Three mIF panels were used in this study (IP1.2 ...
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bioRxiv - Developmental Biology 2024Quote: ... slides were incubated for 30 minutes at RT with primary Ab which was diluted in TNB buffer (Akoya Biosciences, FP1020), followed by incubation with secondary antibodies ImmPRESS-HRP anti mouse/rabbit (Akoya Biosciences ...
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bioRxiv - Developmental Biology 2024Quote: ... nuclei were counterstained with DAPI (Akoya Biosciences, FP1490) for 5 minutes at RT before mounting the stained tissue with mounting medium (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... a different blocking buffer was used (for mouse and rabbit primary Ab: Akoya Biosciences, ARD1001EA; for rat primary Ab: Akoya Bioscience, 30024). Next ...
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bioRxiv - Developmental Biology 2024Quote: ... Stained sections were imaged using a slide scanner (Akoya vector Polaris).
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bioRxiv - Developmental Biology 2024Quote: ... All Opal reagents were diluted 1:150 in an amplification buffer (Akoya Biosciences, FP1609) except Opal 780 ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by incubation with secondary antibodies ImmPRESS-HRP anti mouse/rabbit (Akoya Biosciences, ARH1001EA) or ImmPRESS-anti rat HRP (Akoya Biosciences ...
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bioRxiv - Developmental Biology 2024Quote: ... an Opal fluorophore working solution (Akoya Biosciences, NEL871001KT) was applied for 10 minutes at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... or ImmPRESS-anti rat HRP (Akoya Biosciences, 30032) for 10 minutes at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... a tyramide fluorescent substrate (OpalTM520, Akoya Biosciences) was used to incubate the embryos ...
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bioRxiv - Cancer Biology 2024Quote: We also performed multiplex immunofluorescence using The PhenoCycler-Fusion system (Akoya Biosciences) at the Spatial Proteomics Facility at SciLifeLab ...
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bioRxiv - Immunology 2024Quote: ... Slides were acquired on an automated slide scanning brightfield microscope (Vectra 3, Akoya Biosciences) and quantified using InForm software (Akoya Biosciences).
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bioRxiv - Microbiology 2024Quote: ... stained with hematoxylin and eosin (H&E) and scanned at 40X magnification using multispectral automated PhenoImager (Akoya Biosciences) for histopathological evaluation ...
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bioRxiv - Microbiology 2024Quote: ... Each antibody was optimized using Opal 3-Plex Anti-Rb Detection Kit (Akoya Biosciences Inc. cat# NEL830001KT) and stained with automated LabSatTM Research (Lunaphore Technologies SA ...
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bioRxiv - Microbiology 2024Quote: ... and analyzed for several immune cell populations using the inForm® tissue Finder (Version 2.4.8) and PhenochartTM (Version 1.0.12) software (Akoya Biosciences).
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bioRxiv - Microbiology 2024Quote: ... Slides were scanned using multispectral automated PhenoImagerTM (Akoya Biosciences) and analyzed for several immune cell populations using the inForm® tissue Finder (Version 2.4.8 ...
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bioRxiv - Neuroscience 2024Quote: ... and Opal 690 (OP-001006) (Akoya, Marlborough, MA)] were diluted in TSA buffer (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... then blocked with tyramide signal amplification (TSA) blocking solution (Akoya Bioscience FP1012) for 20 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Slides were scanned at 20x resolution using automated multispectral microscopy system Vectra 3.0 or PhenoImager HT (Akoya Biosciences) and the Vectra 3.0.5 or Vectra Polaris 1.0 software (Akoya Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... Advanced Cell Diagnostics) and labeled using 1:1500 diluted Opal 570 and 690 dyes respectively (Akoya Biosciences). Labeled sections were stained with DAPI ...
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bioRxiv - Neuroscience 2024Quote: ... Amplification steps were performed as instructed by manufacturer and signal was developed using HRP-C1 followed by incubation with Opal 570 (Akoya FP1488001KT) with the following dilutions ...
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bioRxiv - Neuroscience 2024Quote: ... the Opal™ 620 Reagent Pack (FP1495001KT, Akoya Biosciences Inc) for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... Opal dyes (Akoya #FP1487001KT) at 1:750 dilution were used for signal detection ...
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bioRxiv - Neuroscience 2024Quote: ... and Opal 690 (Akoya Biosciences cat# NC1605064) dyes were used to fluorescently label the probes ...
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bioRxiv - Neuroscience 2024Quote: ... and Opal 520 (Akoya Biosciences cat# NC1601877), Opal 570 (Akoya Biosciences cat# NC1601878 ...
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bioRxiv - Neuroscience 2024Quote: ... Opal 570 (Akoya Biosciences cat# NC1601878) and Opal 690 (Akoya Biosciences cat# NC1605064 ...
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bioRxiv - Neuroscience 2024Quote: ... and blocked in blocking solution composed of 0.5% blocking reagent powder (AKOYA Biosciences, SKU FP1012) in TNB buffer (0.1M Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... Fluorescent visualization of hybridized probes was achieved using Opal 690 or Opal 570 (Akoya Biosciences). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... and the RNAscope LS multiplex fluorescent reagent kit (Advanced Cell Diagnostics, Bio-Techne) with Opal fluorophore reagent pack detection (Akoya BioSciences, Inc.). All slides were counterstained with DAPI and coverslipped with ProLong Diamond antifade mountant (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... and opal dyes (520 nm for channel 1; 570 nm for channel 2; 690 nm for channel 3; Akoya Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... and Opal™ 570 (Akoya Biosciences, #FP1488001KT). Immunohistochemistry Part B was then performed by incubation with ...
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bioRxiv - Neuroscience 2024Quote: ... A series of 3 amplification steps were performed along with the development of fluorescent signal using Opal™ 520 (Akoya Biosciences, #FP1487001KT), and Opal™ 570 (Akoya Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: ... Tyramide signal amplification (TSA) fluorophores (Akoya Bioscience ...
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bioRxiv - Neuroscience 2024Quote: ... antibodies to evaluate the co-expression of ELANE with microglia or neutrophil using an Opal 3-Plex Manual Detection kit (# NEL810001KT, Akoya Bioscience, Marlborough, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... 3222401L13Rik was labeled using the RNAscope® Multiplex Fluorescent Reagent Kit v2 (Acd Bio) with probes designed to target the lncRNA and TSA Plus Cyanine 5 (Akoya Biosciences; 1:750) for detection ...
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bioRxiv - Neuroscience 2024Quote: ... TSA Plus Fluorescein Systems from PerkinElmer (TSA plus Cyanine detection kit, Akoya) was used according to the manufacturer’s protocol for DIG detection ...
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bioRxiv - Genomics 2024Quote: ... and Opal 650 dyes (Akoya Biosciences). Stained sections were imaged with a Perkin Elmer Opera Phenix High-Content Screening System ...
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bioRxiv - Immunology 2024Quote: ... The staining was performed either manually or on automated machine (Opal 6-Plex Detection Kit, Akoya Biosciences, Cat # NEL871001KT). Then the stained tissue sections were mounted with Diamond Anti-fade mounting media and were imaged as whole slides scans on the Vectra (Perkin Elmer) ...
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bioRxiv - Immunology 2024Quote: ... slides were blocked with 1X antibody diluent/block (Akoya Biosciences) for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... The tissues were subjected to HIER either in AR6 or AR9 citrate buffers (Akoya Biosciences). Post-antigen retrieval ...