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Citations for Akoya Biosciences :
201 - 250 of 1480 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... followed by horseradish peroxidase (HRP)-conjugated secondary antibody polymer (Akoya), and HRP-reactive OPAL fluorescent reagents (Akoya ...
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bioRxiv - Bioengineering 2024Quote: ... Akoya Polaris whole slide viewer software (Akoya Biosciences and The Spatial Biology Company ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and Opal 690 fluorophores (Akoya Biosciences). Fluorescent images were captured using a laser scanning confocal microscope (Leica Stellaris 8 Falcon) ...
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bioRxiv - Developmental Biology 2024Quote: ... TSA Cy3 (1:75, Akoya, NEL704A001KT), or TSA Cy5 (1:100 ...
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bioRxiv - Developmental Biology 2024Quote: ... or anti-DNP-HRP (1:200, Akoya, FP1129), respectively ...
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bioRxiv - Developmental Biology 2024Quote: ... or TSA Cy5 (1:100, Akoya, NEL705A001KT). The col1a1 riboprobe was generated by PCR amplification of cDNA with primers 5′-GTATTGTAGGTCTCCCTGGACAAA-3′ and 5′-TGTTCTTGCAGTGGTATGTAATGTT-3′ ...
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bioRxiv - Developmental Biology 2024Quote: ... Opal 570 dye (Akoya Biosciences) was used for channel development.
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bioRxiv - Developmental Biology 2024Quote: ... Opal-Tm 570 (Akoya Biosciences, FP1488001KT) fluorophore diluted 1:1500 in TSA plus buffer (ACD ...
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bioRxiv - Cancer Biology 2024Quote: We also performed multiplex immunofluorescence using The PhenoCycler-Fusion system (Akoya Biosciences) at the Spatial Proteomics Facility at SciLifeLab ...
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bioRxiv - Cancer Biology 2024Quote: Multiplex staining was performed using the Opal 6-Plex Manual Detection kit (Akoya Biosciences, NEL861001KT). Procedures were conducted according to the protocol with a deviation ...
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bioRxiv - Neuroscience 2024Quote: ... 3222401L13Rik was labeled using the RNAscope® Multiplex Fluorescent Reagent Kit v2 (Acd Bio) with probes designed to target the lncRNA and TSA Plus Cyanine 5 (Akoya Biosciences; 1:750) for detection ...
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bioRxiv - Immunology 2024Quote: ... Slides were scanned at 20x resolution using automated multispectral microscopy system Vectra 3.0 or PhenoImager HT (Akoya Biosciences) and the Vectra 3.0.5 or Vectra Polaris 1.0 software (Akoya Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... then blocked with tyramide signal amplification (TSA) blocking solution (Akoya Bioscience FP1012) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and Opal 690 (OP-001006) (Akoya, Marlborough, MA)] were diluted in TSA buffer (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... TSA-Cy3 Plus kit (Akoya #NEL744001KT) was used to detect anti-DIG antibody at 1:200 ...
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bioRxiv - Microbiology 2024Quote: RNAscope® ISH slides were scanned at 40X magnification using an Akoya PhenoImager HT whole slide scanner (Akoya Biosciences, Cambridge, MA, USA). Quantitative analysis was performed in QuPath 0.5.1 digital pathology image analysis software [37] ...
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bioRxiv - Neuroscience 2024Quote: ... and 690 (FP1497001KT, Akoya Bioscience) in a 1:1500 dilution were used to mark Channel 2-3 ...
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bioRxiv - Neuroscience 2024Quote: ... Opal dyes 570 (cat. no. FP1488001KT, Akoya Bioscience), and 690 (FP1497001KT ...
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bioRxiv - Neuroscience 2024Quote: ... and Opal 690 (Akoya Biosciences cat# NC1605064) dyes were used to fluorescently label the probes ...
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bioRxiv - Neuroscience 2024Quote: ... and Opal 520 (Akoya Biosciences cat# NC1601877), Opal 570 (Akoya Biosciences cat# NC1601878 ...
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bioRxiv - Neuroscience 2024Quote: ... Opal 570 (Akoya Biosciences cat# NC1601878) and Opal 690 (Akoya Biosciences cat# NC1605064 ...
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bioRxiv - Neuroscience 2024Quote: ... and blocked in blocking solution composed of 0.5% blocking reagent powder (AKOYA Biosciences, SKU FP1012) in TNB buffer (0.1M Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... Opal dyes (Akoya #FP1487001KT) at 1:750 dilution were used for signal detection ...
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bioRxiv - Neuroscience 2024Quote: ... Fluorescent visualization of hybridized probes was achieved using Opal 690 or Opal 570 (Akoya Biosciences). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... H&E and IHC slides were scanned using a Phenolmager whole slide scanner (Akoya Biosciences, Malborough, MA, USA) for figure preparation ...
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bioRxiv - Neuroscience 2024Quote: ... then the appropriate Opal dye (520, 570, 620, or 690; Akoya Biosciences, Marlborough, MA) for 30 min ...
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bioRxiv - Systems Biology 2024Quote: ... and Opal 620 Reagent Pack (Akoya Biosciences, Cat. No. FP1495001KT) were used (1:500 dilution in TSA Buffer).
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bioRxiv - Systems Biology 2024Quote: ... the Opal 520 Reagent Pack (Akoya Biosciences, Cat. No. FP1487001KT) and Opal 620 Reagent Pack (Akoya Biosciences ...
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bioRxiv - Neuroscience 2024Quote: Opal 650 (Akoya Biosciences Cat No. FP1496A) detection at 1:750 dilution of Trpc5 or Fos ...
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bioRxiv - Neuroscience 2024Quote: ... and Opal 690 (Akoya Biosciences Cat No. OPI-001006) detection at 1:3000 dilution of POMC were performed on the Bond Rx according to the ACD protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Opal 570 (Akoya Biosciences Cat No. OPI-001003) detection at 1:750 of Cacna1g ...
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bioRxiv - Neuroscience 2024Quote: ... on sections and performed TSA Plus biotin system (catalog. no. NEL749A001KT, Akoya Biosciences). Then ...
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bioRxiv - Neuroscience 2024Quote: ... and TSA Plus Cy3 System (TSA-Plus Cyanine 3 system, catalog. no. NEL744001KT, Akoya Biosciences, Marlborough, MA). After inactivation of Cy3 system by 3% H2O2 ...
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bioRxiv - Neuroscience 2024Quote: ... Epitope retrieval/microwave treatment (MWT) for all antibodies was performed by boiling slides in Antigen Retrieval buffer 6 (AR6 pH6; Akoya, AR6001KT). Protein blocking was performed using antibody diluent/blocking buffer (Akoya ...
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bioRxiv - Neuroscience 2024Quote: Images were acquired on a Vectra Polaris Automated Quantitative Pathology Imaging System (Akoya Biosciences) at 20· magnification ...
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bioRxiv - Neuroscience 2024Quote: ... and image tiles were spectrally unmixed using inForm Tissue Analysis software (Akoya Biosciences). Stitching of spectrally unmixed image tiles and image analyis were performed in QuPath software95 ...
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bioRxiv - Neuroscience 2024Quote: ... Regions of interest (ROIs) were selected using Phenochart software (Akoya Biosciences) and image tiles were spectrally unmixed using inForm Tissue Analysis software (Akoya Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... followed by opal 570 signal amplification system (opal 570; Akoya, FP1488001KT). Nuclei were stained with DAPI (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Antigen retrieval was performed using AR9 (EDTA) buffer from Akoya Biosciences in a low-pressure cooker for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... the slides underwent a PBS wash and were promptly transferred to the FCAD Machine (Akoya Biosciences) for flow cell mounting ...
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bioRxiv - Cell Biology 2024Quote: ... A 250 microliters of each specific report stock solution were pipetted into a 96 well-plate that was sealed using an aluminum foil provided by Akoya Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... we used the PhenoCycler Fusion 2.0 from Akoya Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... The immunostained slides were scanned at (40X) using the PhenoImager HT instrument (Akoya Biosciences). For each slide ...
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bioRxiv - Developmental Biology 2024Quote: ... and Opal 650 (Akoya Biosciences) and TSA-biotin (TSA Plus Biotin Kit ...
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bioRxiv - Immunology 2024Quote: ... The Opal 3-Plex Anti-Rb Manual Detection Kit (Akoya Biosciences ...
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bioRxiv - Immunology 2024Quote: ... an analysis algorithm was built using InForm software 2.6.0 (Akoya Biosciences, Marlborough, USA) with representative regions across different samples ...
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bioRxiv - Immunology 2024Quote: ... An antigen-retrieval (AR) step by incubation in citric acid buffer pH6 (Akoya Biosciences, Marlborough, USA) for 1 min at 1000 W and 10 min at 100 W was used to remove primary antibodies and HRP polymer before entering the next staining cycle ...
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bioRxiv - Immunology 2024Quote: ... Primary antibodies were detected by incubation for 10 min with HRP-Polymer (Akoya Biosciences, Marlborough, USA) and subsequent TBST washing for 3x 3 min ...
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bioRxiv - Immunology 2024Quote: ... The image analysis algorithm consisted of several steps including spectral unmixing based on proprietary spectral libraries of designated fluorochromes as supplied by Akoya Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Primary antibodies were diluted using antibody diluent (Akoya Biosciences, Marlborough, USA) and incubated for 45 min at RT followed by 3x 3 min washing with 1x TBST ...