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Citations for Akoya Biosciences :
601 - 650 of 1480 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and Opal 650 (FP1496001, Akoya Biosciences). Next ...
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bioRxiv - Neuroscience 2023Quote: ... Opal 570 (FP1488001, Akoya Biosciences) and Opal 650 (FP1496001 ...
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bioRxiv - Cell Biology 2023Quote: ... TSA fluorophores were diluted in multiplex TSA buffer (ACD 322809) Cy5 (Akoya TB-000203) at 1:2000 ...
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bioRxiv - Cancer Biology 2023Quote: Images were acquired using the Mantra™ Quantitative Pathology Work Station (Akoya Biosciences). A minimum of ten images were acquired from each tissue seciton ...
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bioRxiv - Cancer Biology 2023Quote: ... images were analyzed using inForm® Cell Analysis™ software (Akoya Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... and imaging were completed in accordance with Phenocycler manufacturer instructions (Akoya Biosciences User Manual, Revision-C)38 ...
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bioRxiv - Neuroscience 2023Quote: ... The signals were amplified by TSA Fluorescein system (Akoya Biosciences, Cat. no. NEL701A001KT) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The tissue sections were then fixed and stored at 4℃° C in Storage Buffer (Akoya Biosciences, MA) until image acquisition ...
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bioRxiv - Molecular Biology 2023Quote: ... Images from the stained tissue sections were acquired on the CODEX instrument (currently called Phenocycler, Akoya Biosciences, MA), attached to a Keyence BZ-X810 fluorescent microscope (Keyence ...
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bioRxiv - Molecular Biology 2023Quote: For antibodies purchased from Akoya Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... The tissue sections were then washed and equilibrated to room temperature in Staining Buffer (Akoya Biosciences, MA), and then incubated with the complete antibody cocktail overnight at 4° C ...
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bioRxiv - Molecular Biology 2023Quote: ... and sixteen were conjugated at the staining site (Enable Medicine Laboratory) following a protocol provided by Akoya Biosciences (https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf) ...
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bioRxiv - Molecular Biology 2023Quote: ... conjugates were purified by four rounds of centrifugation and buffer exchange with Purification Solution (Akoya) through 50 kDa molecular weight cutoff filters (MilliporeSigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... five conjugated antibodies were purchased from Akoya Biosciences ...
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bioRxiv - Genomics 2023Quote: ... The spatial proteomic datasets were generated by Akoya Biosciences and Nanostring Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... nuclei were stained with DAPI (Akoya Biosciences, NEL840001KT) and slides were mounted using ProLong Diamond Antifade Mountant (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections underwent rounds of antigen stripping via incubation at 100 °C in AR9 buffer (Akoya Biosciences, AR900250ML) for 25 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated with anti-rabbit HRP-conjugated secondary antibody (Akoya Biosciences, NEL840001KT). Sections underwent rounds of antigen stripping via incubation at 100 °C in AR9 buffer (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then cooked in a steam cooker with antigen retrieval buffer pH9 (Akoya Biosciences, AR900250ML) at 100 °C for 25 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated with blocking buffer (Akoya Biosciences, NEL840001KT) for 10 minutes at room temperature and incubated with the primary antibody for the indicated time and concentration (Key Resource Table) ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation times and appropriate antibody concentrations were optimized using the Opal™ 5-color kit (Akoya Biosciences, NEL840001KT). Epitope stability was assessed to determine the order of each antibody in sequence ...
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bioRxiv - Cancer Biology 2023Quote: ... each round with a different OpalTM dye (Akoya Biosciences, NEL840001KT). Finally ...
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bioRxiv - Cell Biology 2023Quote: Multispectral whole slide images were acquired on a Vectra Polaris imaging system (Akoya Biosystems) using a 40x objective ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorescent detection was performed using an Opal fluorophore tyramide-based signal amplification system (Akoya Biosystems) with the following combinations ...
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bioRxiv - Cancer Biology 2023Quote: ... required the tyramide signal amplification (TSA) biotin system following the manufacturer’s protocol (AKOYA Biosciences #NEL 700A001KT). Slides were counterstained with hematoxylin (Ventana 760-2021 ...
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bioRxiv - Developmental Biology 2023Quote: ... Fluorescent RNA in situ hybridization was done with DIG-labeled probes for white and yellow with TSA Plus Fluorescence Kits (Akoya Biosciences) and used for in situ hybridization to imaginal discs as described (Tian et al ...
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bioRxiv - Developmental Biology 2023Quote: ... and Tyramide signal amplification (Akoya Biosciences) was performed according to manufacturer’s instruction ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... diluted 1:50 in amplification buffer (1X Plus Amplification Diluent, Akoya Biosciences, FP1135). Next ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... the antibody solution was washed off using PBST and the antibody was developed in dark for 45 minutes without agitation using a tyramide staining solution (TSA PLUS Fluorescein Reagent, Akoya Biosciences, TS-000200) diluted 1:50 in amplification buffer (1X Plus Amplification Diluent ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... Digoxygenin staining was then developed using the Tyramide PLUS staining solution (TSA PLUS Cy3 reagent, Akoya Biosciences, TS-000202) diluted 1:50 in Amplification buffer for 45 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Mixed RNAscope (Advanced Cell Diagnostics)/antibody antigen retrieval and staining with Opal (Akoya Biosciences) fluorophores was performed manually following the RNAscope Multiplex Fluorescent v2 Assay combined with the immunofluorescence protocol (322818-TN ...
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bioRxiv - Immunology 2023Quote: ... Fluorescent multiplex IHC was achieved using Opal fluorochromes 570 and 690 (Akoya Biosciences ...
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bioRxiv - Immunology 2023Quote: ... (Akoya Biosciences, #NEL741001KT) and C3 oligos with TSA-Biotin (Akoya Biosciences ...
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bioRxiv - Immunology 2023Quote: ... and HRP-C4 with Opal 520 (Akoya, OP-001001) diluted 1:3000 ...
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bioRxiv - Immunology 2023Quote: ... The same steps were repeated for HRP-C2 with Opal 690 (Akoya, OP-001006) and HRP-C4 with Opal 520 (Akoya ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorophores used were Opal 520 reagent (Akoya Biosciences, #OP-001001), Opal 570 reagent (#OP-001003) ...
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bioRxiv - Cancer Biology 2023Quote: ... All antibodies not commercially available from Akoya and hence requiring custom-conjugation with DNA-barcodes were first validated with conventional immunofluorescence (Supplementary Note 2) ...
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bioRxiv - Neuroscience 2023Quote: ... An annotation was drawn to outline each entire slide in a given QPTIFF using Phenochart whole slide viewer (Akoya Biosciences). Spectral fingerprints ...
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bioRxiv - Neuroscience 2023Quote: ... and fused to recreate an entire slide image using Phenochart (Akoya Biosciences), inForm (Akoya Biosciences) ...
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bioRxiv - Neuroscience 2023Quote: ... and TSA plus Cy3 (Red; Akoya Bioscience, Marlborough, MA). Note that the first peroxidase label on the fluorescein probe was completely quenched with 3% H2O2 for 40 mins before the second antibody for the DIG probe was labeled ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with Tris-NaCl-Tween Buffer and incubated with Cy5-tyramide solution (TSA Plus Cyanine 5; AKOYA Biosciences, NEL745001KT) for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with Tris-NaCl-Tween Buffer after incubation with anti-DIG-POD antibodies and incubated with Cy3-tyramide solution (TSA Plus Cyanine 3; AKOYA Biosciences, NEL744001KT) for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Stained sections were scanned using the Vectra multispectral imaging system and viewed using PhenoChart 1.1.0 Software (Akoya BioSciences® ...
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bioRxiv - Cancer Biology 2023Quote: ... Images were then imported to Phenochart (11.0, Akoya Biosciences) and Inform Viewer (2.5.0 ...
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bioRxiv - Neuroscience 2023Quote: ... and RNAscope fluorophores (Opal dyes, Akoya Biosciences) used were 520 ...
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bioRxiv - Neuroscience 2023Quote: ... Cy3 or fluorescein at 1:1000 in TSA buffer (TSATM Plus System, Akoya Bioosciences). Following that slides were incubated in HRP blocker for 15 min and then rinsed 3 times with wash buffer reagent ...
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bioRxiv - Neuroscience 2023Quote: ... was carried out as described by the supplier using tshr2 (ENSORLG00000014222) and cyp19a1b (ENSORLG00000005548) probes and combined with opals 520 (tshr2) and 690 (cyp19a1b) (Akoya Bioscience) to avoid signal crosstalk during imaging.
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bioRxiv - Neuroscience 2023Quote: ... for C1 and Opal 570 Reagent (1:750; Akoya Biosciences, #FP1488001KT) for C3 ...
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bioRxiv - Immunology 2023Quote: ... signal amplification with Opal 650 Dye (Akoya Biosciences, OP-001005) in RNAscope Multiplex TSA Buffer ...
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bioRxiv - Neuroscience 2023Quote: ... and 520 (Akoya Biosciences) were used for fluorescence and after the final HRP block step ...