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Citations for Akoya Biosciences :
1 - 50 of 1480 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... tissue sections were incubated with 1X Antibody Diluent/Block (Akoya Biosciences) for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... InForm software (Akoya Biosciences) was used to remove autofluorescence and cleanly separate signals from each target into individual channels ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were scanned with the Vectra® 3 (Akoya Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... Spectral unmixing of images was performed using the inform software package (Akoya Biosciences).
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bioRxiv - Cancer Biology 2024Quote: ... Scanning of the full antibody panel was performed on PhenoImager (Akoya Biosciences). A spectral fluorophore for each primary antibody and autofluorescence library was made to enable optimal multispectral unmixing ...
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bioRxiv - Cancer Biology 2024Quote: ... Staining was performed on a Leica BOND RX autostainer according to the modified Opal 7-color (v5.2 plus) preset protocol using the Opal 6-Plex Detection kit for Whole Slide Imaging (Akoya Biosciences). Prior to the first staining round ...
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bioRxiv - Cancer Biology 2024Quote: ... All optimization images were unmixed and assessed in inForm V2.6 and Phenochart V1.1.0 (Akoya Biosciences).
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bioRxiv - Cancer Biology 2024Quote: Slides were scanned on PhenoImager HT (Akoya Biosciences) in 4-color motif mode ...
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bioRxiv - Cancer Biology 2024Quote: ... Images were processed with inForm software (Akoya Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: Stained FFPE slides were scanned on a Vectra® Polaris™ Imaging System (Akoya, Biosciences, Marlborought, MA, USA) at X20 magnification ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then visualized with Phenochart (1.0.12 version, AKOYA Biosciences) for multispectral imaging and were annotated and quantified using inForm Tissue Analysis Software (Akoya ...
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bioRxiv - Cancer Biology 2024Quote: ... for multispectral imaging and were annotated and quantified using inForm Tissue Analysis Software (Akoya, Biosciences, Marlborought, MA, USA). The whole tumor area including the invasive margin ...
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bioRxiv - Developmental Biology 2024Quote: ... and Cyanine 3 (Akoya Biosciences NEL744001KT) diluted 1:500 in RNAscope Multiplex TSA Buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... Signals for the transcripts were detected using fluorescein (Akoya Biosciences NEL741001KT) and Cyanine 3 (Akoya Biosciences NEL744001KT ...
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bioRxiv - Neuroscience 2024Quote: ... FITC detection was performed using TSA Plus fluorescein diluted 1:50 in amplification diluent (NEL741001KT, Akoya Biosciences). Samples were washed several times in TNT ...
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bioRxiv - Cell Biology 2024Quote: ... 15 minutes of Protease III (ACD) at 40°C and 1:750 dilution of OPAL570 or OPAL690 reagents (Akoya Biosciences, Marlborough, MA). Immunohistochemistry for claudin-5 co-localization was performed after RNAScope staining ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were stained with DAPI (Akoya Biosciences®). Slides were scanned using the “Vectra Polaris” platform (Akoya Biosciences®) ...
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bioRxiv - Cancer Biology 2024Quote: ... Spectral unmixing and further tissue /cell segmentation as well as phenotyping were performed using the inForm Advanced Image Analysis software (inForm v2.4.10, Akoya Biosciences®). Multiplex data analyses ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were scanned using the “Vectra Polaris” platform (Akoya Biosciences®). Spectral unmixing and further tissue /cell segmentation as well as phenotyping were performed using the inForm Advanced Image Analysis software (inForm v2.4.10 ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was washed several more times in TNT and DIG detection was performed using TSA Plus Cyanine diluted 1:50 in amplification diluent (NEL744001KT, Akoya Biosciences). Fluorescently labeled samples were imaged using confocal microscopy.
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bioRxiv - Cancer Biology 2024Quote: CODEX staining was done using the sample kit for PhenoCycler-Fusion (Akoya, 7000017) according to Akoya’s PhenoCycler-Fusion user guide with modifications to include a photobleaching step and overnight incubation with antibodies at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... sample slides were washed 3 times in PBS and incubated in final fixative solution (1000uL of PBS + 20uL of Akoya’s final fixation reagent) for 20 minutes at room temperature.
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bioRxiv - Cancer Biology 2024Quote: ... diluted 1:300 in 1X Plus Automation Amplification Diluent (Akoya Biosciences, #FP1609). All steps were carried out at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... a 5 min DAPI staining (Akoya Biosciences, #FP1490) was performed before mounting with ProLong Diamond Antifade media (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... the 6-plex TSA with Opal dyes (Akoya) 6-plex and the 8-plex Signal Star (CST) ...
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bioRxiv - Cancer Biology 2024Quote: mIHC staining was performed according to the manufacturer’s instructions (OpalTM 4-Color Anti-Rabbit Automation IHC; OpalTM Polymer Anti-Rabbit HRP Secondary Antibody Kit; Akoya Biosciences®). The following primary rabbit antibodies were used for staining ...
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bioRxiv - Cancer Biology 2024Quote: ... Each slide was scanned using the Vectra Polaris (Akoya Biosciences) imaging platform and the Phenochart software (PerkinElmer) ...
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bioRxiv - Cancer Biology 2024Quote: ... Images were taken on the Vectra Polaris Automated Quantitative Pathology Imaging System (Akoya Biosciences) at 20X magnification ...
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bioRxiv - Neuroscience 2024Quote: ... Whole slides were scanned using a Vectra Polaris System (Akoya Bio). After spectral unmixing ...
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bioRxiv - Developmental Biology 2024Quote: ... 620 and 690 (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1:750 dilution of TSA-Cyanine 3 Plus (AKOYA). The RNAscope® 3-plex LS Multiplex Negative Control Probe (Bacillus subtilis dihydrodipicolinate reductase (dapB ...
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bioRxiv - Neuroscience 2024Quote: ... The following Opal Dyes were used: Opal 520 reagent pack (FP1487001KT; Akoya Biosciences), Opal 570 reagent pack (FP1488001KT) ...
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bioRxiv - Neuroscience 2024Quote: ... Cdh22 (ACDBio #573541-C3) and Cntn6 (ACDBio #836461) mRNA was visualized using Opal 570 (Akoya BioScience, FP1488001KT) and Opal 650 (Akoya BioScience ...
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bioRxiv - Neuroscience 2024Quote: ... and Opal 650 (Akoya BioScience, FP1496001KT).
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bioRxiv - Neuroscience 2024Quote: ... mRNAs of interest were labeled with the following fluorophores (Akoya): Opal 520 (FP1487001KT) ...
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bioRxiv - Neuroscience 2024Quote: ... Final labeling was realized using 570 Opal fluorophore (FP1488001KT, Akoya Biosciences, Marlborough, MA, USA) for Ntrk2 mRNA and 690 Opal fluorophore ...
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bioRxiv - Immunology 2024Quote: Multiplexed immunohistochemistry (mIHC) assay was performed using the Opal 6-Plex Detection Kit (AKOYA #811001, USA) as described previously73 ...
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bioRxiv - Neuroscience 2024Quote: ... The signals were amplified by a 1:70 TSA-plus Cyanine 3 (#NEL744001KT; AKOYA Bioscience) for 25 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... HRP-conjugated anti-flu antibody (at a dilution of 1:250; #NEF710001EA, AKOYA Bioscience) was used to detect Flu-labeled RNA probes by a 1:70 TSA-plus biotin (#NEL749A001KT ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated in TSA-plus biotin (NEL749A001KT; AKOYA Bioscience) at a 1/1500 concentration for 30 minutes at 40 °C ...
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bioRxiv - Neuroscience 2024Quote: ... sections following HPR-C1 treatment were incubated with TSA-plus Cyanine 3 (#NEL744001KT; AKOYA Bioscience) at a 1/1500 concentration for 30 minutes at 40 °C ...
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bioRxiv - Neuroscience 2024Quote: ... was used to detect Flu-labeled RNA probes by a 1:70 TSA-plus biotin (#NEL749A001KT; AKOYA Bioscience), which was then visualized with streptavidin-AF488 (at a dilution of 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were subsequently incubated in TSA-plus Cyanine 3 (#NEL744001KT, AKOYA Bioscience) at a 1/1500 concentration for 30 minutes at 40 °C ...
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bioRxiv - Neuroscience 2024Quote: ... mRNA probes were detected using TSA®Plus detection kit (Akoya Biosciences, Marlborough, MA); eGFP was detected with Fluoroscein (1:2000) ...
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bioRxiv - Molecular Biology 2024Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
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bioRxiv - Molecular Biology 2024Quote: ... which generates a single unmixed image tiles using Phenochart (Version 1.2.0, Akoya Biosciences). Images were automatically stored on a secure networked server for further analysis.
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bioRxiv - Molecular Biology 2024Quote: ... Using an Opal 7-Color Automation IHC Kit (Akoya Biosciences), Opal singleplex and multiplex for initially-optimized antibodies AQP1 ...
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bioRxiv - Molecular Biology 2024Quote: ... All multiplex immunofluorescence slides were scanned on a Vectra Polaris Automated Quantitative Pathology Imaging System (Akoya Biosciences) at ×20 magnification ...
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bioRxiv - Neuroscience 2024Quote: ... and 620 fluorescent dyes (1:5000, Akoya Biosystems, FP1487001KT, FP1495001KT). All HRP solutions (C1-C2 ...
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bioRxiv - Neuroscience 2024Quote: ... mRNA was visualized with TSA Vivid Dyes (AKOYA Biosciences) targeting the vesicular GABA transporter (vGAT ...