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Citations for Thermo Fisher :
5101 - 5150 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Cells were again washed with Blocking Buffer 2X for 1 h and imaged in the Heart-Dyno or mounted on microscope slides in ProLong Glass (ThermoFisher Scientific). Low magnification images were taken on a Leica Thunder microscope ...
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bioRxiv - Bioengineering 2024Quote: ... and stained with 2 μg mL-1 Alexa Fluor Plus 647-conjugated donkey anti-rabbit IgG [H+L] and 2 μg mL-1 Hoechst 33342 dye (Invitrogen 62249) for 1 h to label non-internalized beads and DNA ...
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bioRxiv - Cell Biology 2024Quote: ... Tissue was blocked for 1 h using 10% normal goat serum (NGS) and 10% Fc receptor inhibitor (Invitrogen, 14-9161-73) in TBS ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were washed 3X for 10 min in PBST and incubated at RT for 1 h with Alexa- Fluor 546-conjugated donkey anti-rabbit antibody (1:1000; Invitrogen, A10040) in blocking solution ...
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bioRxiv - Biochemistry 2024Quote: 3 l of cells were harvested after 16-18 h of growth by centrifugation at 8000 x g for 10 min at 4°C (Sorvall LYNX 6000, Thermo Fisher) and resuspended in 100 ml of 50 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2024Quote: ... equipped with a heated electrospray probe (HESI II) coupled to a U-HPLC Vanquish H (Thermo Fisher Scientific, Hemel Hempstead, U.K.). Hilic separation mode was done using a Poroshell 120 Hilic-Z (2.7µm ...
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bioRxiv - Biophysics 2024Quote: ... The cells were allowed to attach to the grids for 4-5 h before vitrification in liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific). The following settings were used ...
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bioRxiv - Biochemistry 2024Quote: ... Western-blot membranes were blocked with 1% BSA in Tris-Buffered Saline-Tween (TBST) for 1 h and then incubated with monoclonal HRP anti-flag tag antibody (1:5000 dilution, Thermo Fisher) or with primary (rabbit polyclonal ...
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bioRxiv - Cell Biology 2024Quote: ... Incubate at 31°C for 20 min to 1 h with agitation at 1000 rpm using a Thermomixer (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2024Quote: ... in screening medium were added to the cells for 2 h before mounting the coverslips with ProLong Antifade Kit (ThermoFisher Scientific). Cell nuclei were visualized with Hoechst solution (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were seeded on the polystyrene 24-well trays (Alkali Scientific) for 16 h in the basal medium and transfected with 0.8 μg DNA/well using lipofectamine 2000 (Thermo Fisher Scientific) in DMEM with 10% FBS.
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bioRxiv - Bioengineering 2024Quote: ... incubated at 37°C for 48 h prior to CD3 isolation and activation with human T-Activator Dynabeads (Life Technologies 11131D) at a 3:1 bead:cell ratio for 24 h ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA conversion was conducted from 2 µg purified RNA using the Maxima H Minus first-strand cDNA synthesis kit (cat. no. K1652, ThermoFisher Scientific). Quantitative PCR analysis was done on 500 ng cDNA mixed with 1X Maxima SYBR Green/ROX qPCR Master Mix (cat ...
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bioRxiv - Cancer Biology 2024Quote: For transient siRNA transfection adherent cells were seeded at 6*104 or 8*104 per cm2 24 h prior to transfection and transfected using Lipofectamine RNAiMax transfection reagent (cat.#13778150, Thermo Scientific) or DharmaFect1 transfection reagent (Horizon ...
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bioRxiv - Cell Biology 2024Quote: ... that were previously coated for 1 h at room temperature with 6 mg/mL human fibronectin diluted in PBS (ThermoFisher Scientific).
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Malaria parasite HOPS/CORVET complexes are critical for endocytosis and invasion organelles functionbioRxiv - Cell Biology 2024Quote: ... gels were incubated for 2.5 h in 500 µl PBS containing 10 µg/ml NHS ester 594 (ThermoFisher Cat. No. A37572) protected from light at room temperature ...
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Berberine is a novel mitochondrial calcium uniporter (MCU) inhibitor that disrupts MCU-EMRE assemblybioRxiv - Cell Biology 2024Quote: The hypoxia/reoxygenation (H/R) injury of NRCMs or H9c2 cells were achieved in incubator (Thermo Fisher Scientific, Madison, WI, USA) filled with 94 % N2 ...
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bioRxiv - Genetics 2024Quote: ... Reverse transcription was performed using a Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase (Thermo Fisher Scientific, Massachusetts, USA). qRT-PCR was performed by a Bio-Rad CFX96 Real-Time PCR system (California ...
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bioRxiv - Developmental Biology 2024Quote: mRNA levels were determined by quantitative reverse transcription PCR (RT-qPCR) using the RevertAid H Minus First Strand cDNA Synthesis kit (Thermo Scientific) followed by quantification in technical triplicates with Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent ...
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bioRxiv - Biophysics 2021Quote: ... coated with poly-D-lysine and transiently transfected with the pIRES2-mCherry-YFPCFTR plasmid using Lipofectamine 2000 (Life Technologies), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... mCherry-actin / GFP-myosin strain (Klingner et al., 2014)) were cultured in Dulbecco’s modified Eagle medium (D-MEM) 1g/l glucose (Invitrogen), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... and the first exon and intron of MCAK genomic DNA were cloned into the pENTR/D-TOPO plasmid (Invitrogen) by overlap extension PCR ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C) and dialyzed using Slide-A-Lyzer Dialysis Cassettes (3,500-D protein molecular weight cutoff; Fisher Scientific, 68035) against buffer D (20mM Hepes-KOH pH 7.9 ...
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bioRxiv - Molecular Biology 2020Quote: ... pyruvate DMEM (4.5 g/L D-glucose, 110 mg/L sodium pyruvate; Gibco™ Thermo Fisher Scientific, Waltham, Massachusetts) supplemented with 10% heat inactivated FBS (Gibco™ Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... coliΔXbaI cell cultures are both induced with 0.5 mM (final concentration) of Isopropyl β-D-1-thiogalactopyranoside (IPTG; Invitrogen), and incubated at 37 °C in the dark for a given period of time before being disrupted ...
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bioRxiv - Cell Biology 2020Quote: ... Glucose uptake assay was measured as previously described using the fluorescent D-glucose analog 2NBDG (200 μM; Molecular Probes)35 ...
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bioRxiv - Bioengineering 2021Quote: ... nucleofected T cells were washed with D-PBS and stained with LIVE/DEAD Fixable Violet Dead Cell Stain (ThermoFisher) for 5 minutes at room temperature in the dark before proceeding with antibody stain.
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bioRxiv - Neuroscience 2021Quote: ... The cell suspension was plated in poly-D-lysine culture flasks containing Dulbecco’s modified Eagle’s medium/F-12 medium (Gibco BRL) and 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... D-001810-10-20) was used also at a concentration of 600 nM (both siRNAs from Dharmacon, Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: ... Purified PCR product was first cloned into an entry vector using the pENTR/D-TOPO Cloning Kit (Invitrogen, #K240020) and then into a destination vector from the Drosophila Gateway vector collection system (Murphy Laboratory ...
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bioRxiv - Microbiology 2020Quote: ... EVs were resuspended in D-MEM (0 % FCS) + fluorescent Bodipy Phosphocholine analogue at 2 μg/mL (D3793, Thermofisher Scientific) and incubated overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... and inserted into a pENTR vector (pENTR™/D-TOPO™ Cloning Kit, Thermo Fisher Scientific, San Jose, USA). The products were transformed into chemically competent TOP10 E ...
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bioRxiv - Neuroscience 2020Quote: Neuronal pathway imaging: The habenulo-interpeduncular projection in zebrafish was visualized using lipophilic tracer DiI (D-3911, Invitrogen, USA). DiI granule was applied to the right and left habenula by placing on the tip of a sharp glass needle followed by incubation at 37°C for 48 hours to allow the dye diffusion to occur15,16.
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bioRxiv - Genomics 2020Quote: ... cells were cultured in a 5% CO2-humidified chamber at 37°C in DMEM (1x, 4.5 g/L D-glucose with pyruvate; #D6429, Gibco, ThermoFisher Scientific), supplemented with 10% fetal bovine serum (#BCS0615 ...
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bioRxiv - Microbiology 2021Quote: A549-ACE2 cells were reverse transfected using 20pmol of Non-targeting siRNA (D-001206-13-20) or IFITM2 siRNA (M-020103-02-0010) and 1μL of RNAi max (Invitrogen). Cells were incubated for 24h prior to a second round of reverse transfection ...
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bioRxiv - Neuroscience 2020Quote: ... cell membranes were loaded with the voltage-sensitive dye D-2- ANEPEQ (JPW1114, 0.2 mg/mL, Invitrogen, Carlsbad, CA) for 30 minutes using a first patch clamp recording and then re-patched a second time with dye free solution as previously described (Canepari et al ...
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bioRxiv - Molecular Biology 2020Quote: ... fixed iAT2s were either stained for cell surface expression of ACE2 (R&D, #AF933, 4-8μg/2.5×106 cells) followed by donkey anti-goat IgG-AF647 (Invitrogen, #A21447) or were permeabilized with saponin buffer (Biolegend ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended in fresh DMEM-10 (Gibco DMEM #11995-065 with 4.5 g/L D-Glucose and 110 mg/L Sodium Pyruvate, 10% Fetal Bovine Serum [HyClone], 1% HEPES [Gibco 1M 15630-080] ...
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bioRxiv - Biophysics 2020Quote: Cells were grown in DMEM growth medium ([+] 4.5 g/L D-Glucose, [+] L-Glutamine, [+] 110 mg/L Sodium Pyruvate, Gibco, supplemented with 10 % FBS ...
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bioRxiv - Biophysics 2020Quote: ... they were keep in DMEM growth medium ([+] 4.5 g/L D-Glucose, [+] L-Glutamine, [+] 110 mg/L Sodium Pyruvate, Gibco, supplemented with 10 % FBS ...
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bioRxiv - Biophysics 2020Quote: MCF7-p95ErbB2 cells described previously1 were grown in DMEM growth medium ([+] 4.5 g/L D-Glucose, [+] L-Glutamine, [+] 110 mg/L Sodium Pyruvate, Gibco, supplemented with 10 % FBS ...
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bioRxiv - Cell Biology 2020Quote: ... referred to herein as INS-1 832/13) were cultured in RPMI 1640 medium with 11.1 mM D-glucose (Invitrogen) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2022Quote: Prion-infected brain homogenate was prepared by homogenizing 30 brains from female C57Bl/6 mice terminally-infected with the ME7 prion strain in Dulbecco’s phosphate buffered saline lacking Ca2+ or Mg2+ ions (D-PBS; Gibco) to produce a pool of 130 ml 10% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... were cultured adherently in DMEM (4.5 g/L D-Glucose) supplemented with 1× MEM NEAA (catalog no. 11140-035, Gibco), 1× GlutaMAX (catalog no ...
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bioRxiv - Neuroscience 2022Quote: ... Cell counts were estimated using a haemocytometer and seeded onto 13mm diameter coverslips in 24 well plates (Trajan) pre-coated with 50μg/ml poly-d-lysine (Gibco) and 40μg/ml mouse type 1 laminin (Gibco ...
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bioRxiv - Plant Biology 2022Quote: ... and terminator (503 bp downstream of stop codon) of H4 (At3g53730) into pENTR/D (ThermoFisher Scientific, Waltham, MA, USA). Site-directed mutagenesis of pH4::H4 in pENTR/D using QuikChange II XL (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... The spectra of the samples were recorded in (400 μl) deuterated chloroform (CDCl3, Acros Organics, 99.5% D, Waltham, MA) with an average of 16 scans ...
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bioRxiv - Neuroscience 2022Quote: ... Poly-D-ly-sine beads are prepared as follows: 30 million 4.5 µm aliphatic amine latex beads (Thermo Fisher) were incubated with poly-D-lysine (P7405 ...
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bioRxiv - Molecular Biology 2022Quote: ... which was rotated at 37 °C for 45 min in 1 mg/mL papain suspended in digestion buffer (5% w/v D-trehalose, 0.05 mM APV, 0.0125 mg/mL DNAse, in HibernateTM-A (Thermofisher, A1247501)) ...
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bioRxiv - Neuroscience 2021Quote: ... a staining solution of 1 mg/mL 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (X-gal, Invitrogen), 1× citratesodium phosphate buffer (pH 6.0) ...