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Citations for Thermo Fisher :
4701 - 4750 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... extracted and ethanol precipitated DNA (20 μg) was run in 1% agarose and Southern transferred (standard procedures) onto Biodyne B nylon membranes (Thermo Scientific, MO). Blots were probed with a 753 bp PCR fragment spanning the AAVS1 site ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed twice with cold PBA and labeled for 20 min on ice with Alexa 488-conjugated anti-mouse IgG1 antibodies (1:400, ThermoFisher reference A21121). For the Refrex1/DC16HA ligand ...
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bioRxiv - Developmental Biology 2020Quote: ... After washing (x3, 20 minutes) embryos were incubated with a secondary antibody Alexa Flour 488 anti-mouse (A21202, Invitrogen, 1:1000, overnight) and washed again (x3 ...
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bioRxiv - Developmental Biology 2021Quote: ... PBS-0.1% Tween-20 washes removed excess primary antibody before application of pre-blocked Alexa 633 goat anti-mouse IgG (Life technologies, A21052) secondary antibody at 1:2000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Eyes were dissected from stage 27 anesthetized embryos and cultured at 20°C for 40 h in 60% L-15 and 1% antibiotic-antimycotic (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... membranes were blocked in 7% milk in 1xTBS and 0.01% Tween-20 and streptavidin-HRP immunostaining (1:5000, Invitrogen cat. #19534-050) was performed at room temperature for 1h in blocking solution ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant containing the secreted Fab was isolated by centrifugation (4000xg for 20 minutes) and applied to 1-2mL of IgG-CH1 affinity resin (Thermo Fisher Scientific) pre-equilibrated with 1xHBS ...
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bioRxiv - Immunology 2022Quote: ... Samples were denatured and loaded onto the Jess plate with appropriate reagents and primary antibodies: Anti-S100a8 Polyclonal Antibody – Rabbit (Fisher PIPA579948) diluted 1:20 and anti-S100a9 Polyclonal Antibody – Rabbit (Invitrogen PA5-79950) diluted 1:50 ...
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Brainstem modulation of nociception by periaqueductal gray neurons expressing the mu-opioid receptorbioRxiv - Neuroscience 2022Quote: ... Sections were subsequently washed three times for 20 min in wash buffer (0.2% triton, in PBS) and incubated in secondary antibodies (Life Technologies, 1:500) at room temperature for 2 h ...
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bioRxiv - Cell Biology 2022Quote: ... 40 μl of end-biotinylated λ-DNA (Davidson et al., 2016) was diluted to 1 pM in DNA buffer supplemented with 20 nM Sytox Orange (ThermoFisher Scientific; S11368) and introduced into flow cells at 5 μl/ min ...
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bioRxiv - Immunology 2024Quote: ... Slides were washed in PBS-Tween (0.5% Tween 20 in 1x PBS) and incubated in goat anti-rat IgG Alexa Fluor® 488 (1:200; A11006; Life Technologies) antibody for 45 min at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... get 20 μL of the target media in a 1-200 μL large orifice pipette tip (02-707-134, Thermo Fisher Scientific) and then drag the hanging microdrop at the end of the tip through the patterned surface ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were pre-extracted with 0.1% Tween-20 in PBS (PBST) for 1 min and then fixed with 4% methanol-free formaldehyde (Thermo Fisher Scientific, 28906) for 20 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... was used to amplify the genes of interest according to the primers mentioned in the Table 1 from 15-20 ng of cDNA in the QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher Scientific) machine ...
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bioRxiv - Biophysics 2023Quote: ... The cell pellet was resuspended in 2 mL of chilled 20 mM Tris 1 mM EDTA (referred to as HLM buffer) containing 1X Pierce protease inhibitor (Thermo Fisher Scientific) and incubated on ice for 10 min before homogenization using a Dounce homogenizer ...
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bioRxiv - Molecular Biology 2023Quote: The newly constructed pcDNA5-FRT-HaloTag7-Flag and pcDNA5-FRT-Rpn13(1–128)-HTP-FLAG-FLAG plasmids were co-transfected with the pOG44 plasmid (Invitrogen #V6005-20) containing Flp recombinase into Flp-In 293 cells (Invitrogen R750-07 ...
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bioRxiv - Neuroscience 2022Quote: ... the electrode area of all HD-MEAs was treated with poly-D-lysine (PDL, 20 μL, 0.1 mg mL-1; A3890401, Gibco, ThermoFisher Scientific, Waltham, USA) for 1 hour at room temperature and then rinsed three times with sterile water ...
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bioRxiv - Cell Biology 2023Quote: ... using a wet transfer (1 x transfer buffer, IMP/IMBA/GMI Molecular Biology Service/ Media Kitchen, 20% EtOH or MeOH, or 1 x NuPage™ transfer buffer, Thermo Fisher, NP0006-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were washed three times with TBS-0.1% Tween 20 and incubated for 1 hour with anti-mouse/rabbit secondary antibodies (ThermoFisher Scientific, Waltham, MA). Subsequently ...
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bioRxiv - Cancer Biology 2022Quote: ... The oligos were annealed in a thermocycler with gradual T reduction from 95°C to 25°C at a rate of 5°C/min and subsequently diluted 1:20 into Nuclease free water (ThermoFisher Scientific AM9938). The pLentiCRISPRv2 plasmid was digested for 1 hr at 55°C with 1U per μg of DNA BsmBI-v2 (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Samples were first induced with 0.5% arabinose for 1.5 hours and were subsequently supplemented with anhydrotetracycline to a final concentration of 500 ng/mL and propidium iodide (1:1000 diluted from 20 mM stock solution, Thermo Fisher Scientific), and incubated further for 2 hours at 37°C without agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were blocked with 1% BSA for at least 20 min and treated with Avidin Alexa Fluor 350 (Invitrogen Molecular Probes) for 20 minutes ...
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bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplicons were resolved on 7.6% TBE-acrylamide gels (PanReac Applichem, A1672) at 210V for 20 min and stained with 1:10000 SBYR Safe in H2O (Life Technologies, S33102) for 3 min ...
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bioRxiv - Cancer Biology 2023Quote: ... each well was rinsed with PBS three times with 20 min incubation at room temperature and stained with secondary antibody goat anti-mouse Alexa Fluor 647 (1:250; Invitrogen #A-21235) at room temperature for 90 min ...
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bioRxiv - Immunology 2023Quote: Ex vivo isolated and rested CD8+ T cells or transduced T cells from splenocytes were incubated with R10 (RPMI 1640 + 10% FBS + 1% penicillin/streptomycin + L-glutamine + 20 U/ml of IL-2; Gibco, Thermo Fisher Scientific) alone or R10 containing PMA/ionomycin for two hours ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with PBS three times for 20 min each and counterstained with Hoechst 33342 (1:10,000, Life Technologies, Cat# H3570) for 20 min to visualize nuclei ...
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bioRxiv - Molecular Biology 2023Quote: ... the slides were blocked with 1% BSA for at least 20 min and treated with Avidin Alexa Fluor 350 (Invitrogen Molecular Probes) for 20 min ...
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bioRxiv - Neuroscience 2024Quote: ... mitotic SH-SY5Y cells were differentiated into non-mitotic neuronal cells by the addition of 20 µM all-trans retinoic acid on day 1 (Molecular Probes/Invitrogen, #207340010). From day 1 onwards ...
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bioRxiv - Microbiology 2024Quote: ... of skimmed milk powder and then incubated for 2 h at RT or overnight at 4 °C in 3 ml of PBS 1X + 0.1% (v/v) Tween-20 in presence of a 1,000x dilution of anti GFP antibody (Rabbit anti-GFP, Invitrogen Thermo Fisher Scientific). After washing ...
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bioRxiv - Molecular Biology 2024Quote: ... and non-specific IgG were incubated for 1 hour at room temperature with 20 µL aliquots of Protein A Dynabeads (Thermo Fisher, USA) mixed with 200 µL of PBST ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein samples were separated by SDS-PAGE ((4-20%; Criterion XT precast gel) with 1× MOPS SDS gel running buffer (NP0001, Invitrogen, Life Technologies Corp). Following electrophoresis ...
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bioRxiv - Cancer Biology 2024Quote: ... De-crosslinking was conducted using 4 µl of 5 M NaCl and 1 µl of 20 mg/ml proteinase K (Thermo Fisher Scientific) for 4 h at 60 °C ...
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bioRxiv - Cell Biology 2024Quote: Primary cells were obtained from muscle biopsies taken from the vastus lateralis of healthy female and male volunteers 43 Myoblasts were allowed to proliferate in growth medium (F12/DMEM, 25 mM glucose, 20% FBS, 1% penicillin-streptomycin (Antibiotic-antimyotic, Thermo Fisher Scientific). To induce differentiation ...
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bioRxiv - Cancer Biology 2024Quote: ... The PVDF membrane was blocked for 1 h with 5% BSA in TBST buffer containing 0.1% Tween 20 followed by overnight incubation at 4 °C with primary antibody for: ERα (MA5-14104, dilution 1:400, Thermo Fisher Scientific) and PR (sc-7208 ...
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bioRxiv - Pathology 2022Quote: RNA extractions of swab material were performed in 96-well plates using Mag Max-96 AI/ND Viral RNA Isolation Kit (Applied Biosystems, Foster City, California) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 25 µl from prepared samples were added to one well of 96 well plate and Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) manual was followed to measure the protein content ...
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bioRxiv - Microbiology 2023Quote: ... then 50 μl of sample or standard was added per well (in duplicate) with premixed beads in a 96-well plate according to the manufacturer instructions (ThermoFisher Procartaplex Human Simplex kit). The plate was incubated overnight on a shaker at 4°C ...
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bioRxiv - Plant Biology 2020Quote: cDNA was synthesized from 2 μg of RNA using random primers in 20 μL reaction with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA).
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bioRxiv - Pharmacology and Toxicology 2022Quote: HBV DNA was extracted from 5 μL of serum using the SMITEST EX-R&D Nucleic Acid Extraction Kit (Medical & Biological Laboratories, CO. LTD, Nagoya, Japan) and dissolved in 20 μL nuclease-free water (Thermo Fisher Scientific Inc., Waltham, MA). Real-time PCR was used to measure the serum HBV DNA concentration using the TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: Young seedlings were incubated in 20 μM 5-ethynyl-2’-deoxyuridine (EdU) (Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit, ThermoFisher Scientific/Invitrogen, Waltham, Massachusetts, USA) made in ddH2O for 30 min at 24 °C ...
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bioRxiv - Plant Biology 2019Quote: ... 500 ng of total RNA was converted into cDNA in a 20 μl reaction using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Life technologies, Thermo Fisher Scientific) according to manufacturer's instructions and subsequently 1/5 diluted in sterile RNAse and DNAse free water ...
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bioRxiv - Immunology 2021Quote: ... washed twice in PBS and incubated for 20 minutes at 4°C with Live/Dead™ Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific, Cat. L34965) at a 1:50 dilution in a volume of 50 μL ...
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bioRxiv - Immunology 2022Quote: ... for at least 20 min and then stained with FluoroMyelin™ Green fluorescent myelin stain solution or BrainStain™ Imaging Kit (Molecular Probes, Invitrogen, Eugene, OR) diluted 1:300 in PBS for 20 min ...
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bioRxiv - Plant Biology 2023Quote: ... thaliana and 2500 ng RNA from Norway spruce in 20 µl reactions using SuperScript™ IV VILO™ cDNA Synthesis Kit (Invitrogen, Carlsbad, CA, US) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: The initial inoculum from the 1st cycle and harvested biomass from the 30th cycle of both evolution experiments was frozen at -20°C until DNA extraction with Invitrogen™ PureLink™ Microbiome DNA Purification Kit (Thermo Fisher Scientific; #A29790). DNA samples were sequenced by Novogene Co ...
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bioRxiv - Biophysics 2022Quote: ... 30,000 cells were seeded into a 24-well plate (NuncMicroWell Plates with Nunclon; Thermo Fisher Scientific, Waltham, MA, USA). At day 0 the transfection was done ...
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bioRxiv - Bioengineering 2021Quote: ... All experiments were performed in 384-well plates (Clear Flat-Bottom Immuno Nonsterile 384-Well Plates, MaxiSorp, Life Technologies) which were sealed (Empore Sealing Tape Pad ...
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bioRxiv - Neuroscience 2020Quote: ... Dissociated hippocampal or cortical neurons were plated at 17,000 cells/well (96-well plate) or 1,000,000 cells/well (6-well plate) in neuron media (Neurobasal medium (ThermoFisher 21103049) supplemented with B27 (ThermoFisher 17504044) ...
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bioRxiv - Cancer Biology 2021Quote: ... The 384-well plate was loaded on the the TaqMan Open Array Human MicroRNA Panel plates (Thermo Fisher, USA) and analyzed using QuantStudio™ 12K Flex (Thermo Fisher ...