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Citations for Thermo Fisher :
4651 - 4700 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... HC-04 hepatocytes (1×105) were seeded onto the bottom of a 96-well plate using IMDM (Life Technologies, 11360-070). Sporozoites were added at an MOI of 0.3 and left to invade hepatocytes for either 5 or 18 hours before being trypsinized to obtain a single cell suspension and transferred to a 96 well round bottom plate ...
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bioRxiv - Immunology 2024Quote: ... as well as transmembrane serine protease 2 (TMPRSS2) were seeded at a density of 1 × 104 cells/well in white-walled 96-well plates (ThermoFisher Scientific) one day prior to usage (day 0) ...
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bioRxiv - Neuroscience 2024Quote: ... cochleae were plated in six-well plates (2-3 explants/well) containing 1 ml media (growth medium DMEM (12430-054,Gibco Life Technologies) combined with 1% FBS (16000-044 ...
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bioRxiv - Cell Biology 2024Quote: ... Intact spheres were subsequently diluted 1:10 in PBS and placed in a 6 well plate (Fisher Scientific, #07-000-646). Using a standard tissue culture microscope and a 20 uL pipet tip ...
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bioRxiv - Molecular Biology 2024Quote: ... HC-04 hepatocytes (1×105) were seeded onto the bottom of a 96-well plate using IMDM (Life Technologies, 11360-070). Sporozoites were added at an MOI of 0.3 and left to traverse hepatocytes for 2.5 hours in the presence of 1 mg ml-1 FITC-labelled dextran (10,000 MW ...
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bioRxiv - Microbiology 2024Quote: MDCK or MDCK SIAT1 cells were seeded in 96-well plates at 1-2 ξ 104 cells/well in DMEM (Gibco) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Systems Biology 2024Quote: ... Purified CD14+ monocytes were seeded at a density of 1 million cells/well in 6-well plates in RPMI 1640 (Life technologies) with 10% serum and 20μg/ml recombinant human MCSF (Peprotech ...
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bioRxiv - Microbiology 2024Quote: ... and added to glass coverslips in a 24-well plate along with 1 µl of Lipofectamine RNAiMAX (Thermo Fisher Scientific 13778030). After a 20 min incubation ...
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bioRxiv - Plant Biology 2019Quote: The DNA coding sequences for the 20 fluorescent protein (FP) genes listed in Table 1 were mobilized into Invitrogen pENTR /D-TOPO cloning vectors (Invitrogen, Carlsbad, CA). Following colony PCR and validation by sequencing ...
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bioRxiv - Biophysics 2019Quote: ... namely 1) epoxylated beads with a diameter of 4.5 μm and 20% iron oxide content (Dynabeads M-450, Invitrogen AG, Carlsbad, CA), and 2 ...
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bioRxiv - Microbiology 2019Quote: ... and cultured in Minimum Essential Medium Alpha Modification (MEM-α, HyClone) with 20% fetal bovine serum (FBS, HyClone) and 1% penicillin-streptomycin (P/S, Gibco Life Technologies). Goat adipose-derived mesenchymal stem cells (gASCs ...
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bioRxiv - Molecular Biology 2022Quote: ... coverslips were washed with PBS-Ca2+/Mg2+ containing 0.1% Tween 20 and incubated with Alexa Fluor 488 donkey anti-rabbit (Invitrogen, A21206; 1:1000) and Alexa Fluor 594 goat anti-mouse (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 2µl lysis buffer with 1U/µl Rnase Inhibitor (RRI) and ERCC spike in mix at a 1:20 dilution (Ambion Cat #4456740). Plates were promptly sealed (Thermo Fisher Scientific #AB0626) ...
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bioRxiv - Molecular Biology 2021Quote: ... the synthesized cDNA was diluted 1:20 and used as a template with Power SYBR Green PCR Master Mix (Applied Biosystems #4367659) on an ABI Prism 7500 detection system (Applied Biosystems) ...
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bioRxiv - Bioengineering 2019Quote: ... siRNA stored at a concentration of 50 μM and stored at −20°C and diluted 1:10 in Opti-MEM cell culture medium (ThermoFisher, Catalog #31985070) to reach a concentration of 5 μM prior to transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were washed three times with PBS supplemented with 0.2% tween-20 and were incubated fluorophore-conjugated secondary antibodies (1:1000 dilutions of mouse-Alexa 488 and rabbit-Alexa647 (Thermo Fisher Scientific)) in Blocking buffer for one hour at room temperature ...
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bioRxiv - Immunology 2019Quote: ... was carried out in 10 mM Tris-HCl – 1 mM EDTA buffer (pH 9) in +99°C for 20 min (PT Module; Thermo Fisher Scientific). Tissue peroxide quenching with 0.9% H2O2 for 15 min was followed by protein blocking with 10% normal goat serum (TBS-NGS ...
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bioRxiv - Cell Biology 2020Quote: ... Flow settings were adjusted to allow individual cells to become encapsulated with single hydrogel beads presenting cell-specific barcoding oligonucleotide primers inside 3-5 nL encapsulation mixture droplets that contain 20 U μL−1 reverse transcriptase (SuperScript III, Thermo Fisher Scientific), First-Strand Buffer (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... was resuspended in 1 volume of NuPage NuPAGE©LDS 2X sample buffer supplemented with 20% NuPAGE© sample reducing agents (Invitrogen). The regular western-blot protocol was then used and 8 μL of crude ...
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bioRxiv - Biochemistry 2021Quote: ... 0.1 mg/ml BSA, 20 mM EDTA and a 1/250 dilution of Quant-iT™ RiboGreen® RNA reagent, ThermoFisher Scientific) after which fluorescence was read in black 384-well plates (GRE384fb 781076 ...
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bioRxiv - Genomics 2021Quote: ... then recirculized in a 20 µL reaction containing 60 ng of DNA and 1 µL of T4 DNA Ligase (Thermo Fisher Scientific) in buffer ...
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bioRxiv - Microbiology 2020Quote: ... Viral cDNA was amplified in a 20 µl reaction mixture containing 1 × Dream Taq Green PCR master mix (Thermo Fisher Scientific, Poland), and primers (500 nM each) ...
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bioRxiv - Microbiology 2020Quote: ... Insoluble material was removed by centrifugation (25,000 × g for 30 min at 20°C) prior to incubation in 1 ml of Ni-NTA agarose (ThermoFisher Scientific, #R901-01) for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... and was maintained in Dulbecco’s Modified Eagle Medium supplemented with 20% Fetal Bovine Serum and 1% Penicillin-Streptomycin solution (all from Gibco, Thermo Fisher Scientific). Primary cell cultures PCB-00007 ...
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bioRxiv - Cancer Biology 2021Quote: ... and was maintained in Dulbecco’s Modified Eagle Medium supplemented with 20% Fetal Bovine Serum and 1% Penicillin-Streptomycin solution (all from Gibco, Thermo Fisher Scientific). The IM-WT-1 ...
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bioRxiv - Cancer Biology 2020Quote: ... with serum-free DMEM supplemented with basic Fibroblast Growth Factor (20 ng/mL) and B-27 supplement (1:50; Thermo Fisher Scientific). Media was supplemented on Day 4 ...
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bioRxiv - Bioengineering 2022Quote: ... Grids were negatively stained with 1% uranyl acetate and observed in a viewed under a Tecnai 20 transmission electron microscope operating at 120 kV (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10 mM ATP in kinase buffer (20 mM Tris pH 8, 150 mM NaCl, 10 mM MgCl2, 1 mM DTT, 1X Phosphatase inhibitor (Thermo Scientific, #1862495)) at room temperature for 2 h ...
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bioRxiv - Genetics 2022Quote: ... and 5 µL of Tagment DNA enzyme diluted 1:20 in Tagment DNA buffer and incubating this mixture on a SimpliAmp thermal cycler using the following parameters (Thermo Fisher Scientific): 55 °C temperature ...
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bioRxiv - Biochemistry 2022Quote: ... the synthesized cDNA was diluted 1:20 and used as a template with Power SYBR Green PCR Master Mix (Applied Biosystems #4367659) on an ABI Prism 7500 detection system (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were subsequently washed three times for 20 min in wash buffer (0.2% triton, in PBS) and incubated in secondary antibodies (Life Technologies, 1:500) at room temperature for 2 h ...
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bioRxiv - Cell Biology 2022Quote: ... The culturing media was reintroduced and either the prepared PS-/PC-beads were added at a ratio of 20:1 (Beads: Cells) or apopHEK cells stained with 5 μM eBioscience CFSE (Invitrogen Thermo Fisher Scientific) were added at a ratio of 5:1 (HEK ...
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bioRxiv - Neuroscience 2021Quote: ... Antibody-bound NLGNs were incubated for 1 hour with 20 µL of protein G beads (Dyna-beads Protein G, Thermo Fisher Scientific) precipitated and washed 4 times with lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... Viral cDNA was amplified in a 20 µl reaction mixture containing 1 × Dream Taq Green PCR master mix (Thermo Fisher Scientific, Poland), and primers (500 nM each) ...
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bioRxiv - Neuroscience 2021Quote: ... Larvae and juveniles were paralyzed by immersion in α-bungarotoxin (20 µl of 1 mg/ml solution in system water, B1601, ThermoFisher Scientific) (Duboué et al. ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then stained with 20 µg/mL biotinylated S protein (S1/S2) or 1:1000 Anti-HS (Clone F58-10E4) (Fisher Scientific, NC1183789) in PBS containing 0.5% BSA ...
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bioRxiv - Microbiology 2021Quote: ... 0.12 U μl−1 Turbo DNase (Albion) or 5 U RNase A and 20 U RNase T1 (RNase Cocktail enzyme mix, Thermo Fisher Scientific) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... blocked for 20 min at room temperature and stained overnight at 4 °C using an anti-V5 antibody (1/500; Invitrogen, Cat R96025) or anti-FLAG antibody (1:100 ...
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bioRxiv - Biochemistry 2020Quote: ... beads were washed three times with 0.5-1 ml of SA-RNP lysis buffer (20 mM Tris-HCl (pH 7.5, Ambion, AM9850G, and Ambion, AM9855G), 150 mM NaCl (Ambion ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated 20 min at 4°C with AF647-conjugated goat anti-human IgG antibodies (1:1000 dilution; Thermo Fisher Scientific) and LIVE/DEAD Fixable Viability dye Aqua (1:1000 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... corresponding to 1 µg was generated using oligo(dT)20 primers and the SuperScript™ III First-Strand Synthesis SuperMix (Invitrogen, 18080400) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... cells were washed 3 times in 0.2% Tween-20/1× PBS and twice in distilled water before mounting in Prolong Diamond (Thermo Fisher Scientific, P36961).
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bioRxiv - Cell Biology 2019Quote: ... with 0.1% Tween 20 to assess endothelial VCAM-1 and E-selectin protein expression using the SuperPicture 3rd Gen Immunohistochemistry detection system (Invitrogen, Waltham, MA). Endogenous peroxidases were quenched and endogenous mouse IgG in blood serum was blocked using the Mouse-on-Mouse blocking reagent (Vector Laboratories ...
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bioRxiv - Bioengineering 2019Quote: ... and the secondary antibody was applied for 20 minutes at room temperature (Alexa Fluor® 488 Donkey anti-mouse, Invitrogen; 1:800). All nuclei were counterstained with DAPI (Vector Labs) ...
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bioRxiv - Plant Biology 2019Quote: ... The run-on assay was performed in a reaction containing 10 µl 10X transcription assay buffer (500 mM pH7.5 Tris-HCl, 50 mM MgCl2, 1.5 M KCl, 1% sarkosyl, 20 U/ml RNase inhibitor [Invitrogen] and 100 mM DTT), 50 µl nuclei in storage buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Chromatin fragments were immunoprecipitated overnight using 1 μg antibody of SOX11-PAb antibody and 20 μl Protein A UltraLink® Resin (Thermo Scientific) beads per 10×106 cells ...
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bioRxiv - Genomics 2021Quote: ... Then these sliced samples were washed with 0.05 % Tween 20 (Nacalai) 3 times and incubated with secondary antibodies (Alexa Fluor 488- and 561-conjugated antibodies, Life Technologies, 1:500) diluted with 0.05 % Tween 20 for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... After 3 washes in wash buffer (0.1% Tween-20 in PBS), second antibodies (Alexa 488-conjugated IgG, Alexa 594-conjugated IgG) containing 1/5000 Hoechst 33342 (Thermo Fisher Scientific) were reacted at room temperature for 2 h ...
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bioRxiv - Developmental Biology 2021Quote: ... approximately 20 pairs of testes per genotype were dissected in TIB with protease inhibitor cocktail (1:100; Halt, Thermo Fisher Scientific, 87786), then lysed in radioimmunoprecipitation assay (RIPA ...
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bioRxiv - Cancer Biology 2020Quote: ... along with 20 nM siRNA (unless dose is otherwise indicated) in a 1:2 ratio with Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific) for 16-24 hours at 37°C in 5% CO2.