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Citations for Thermo Fisher :
4601 - 4650 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cells—FACS-purified from spleens of B6.Rag1−/− 2-3 weeks post naïve T cell transfer—were activated in round-bottom 96-well plates with mouse anti-CD3/anti-CD28 T cell expander beads (1 bead/cell; Life Technologies) in complete media containing 10 U/mL recombinant human (rh ...
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bioRxiv - Molecular Biology 2022Quote: ... Larger scale knockdown samples were produced by reverse transfecting 5 million cells with 35 μL of transfection reagent in 10 cm plates. The control siRNA (siNC; “Silencer® Select Negative Control No. 1 siRNA”) was purchased from Invitrogen™ (cat ...
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bioRxiv - Immunology 2022Quote: ... cells were plated at 1 × 106 cells per well in v-bottom Nunclon Delta treated 96-well plates (Thermo Fisher Scientific) and washed twice with 100 μl ice-cold 1X PBS to remove proteins in the supernatant before being pelleted by centrifugation at 500 × g for 2 min at 4 °C ...
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bioRxiv - Genetics 2022Quote: ... ~1 × 105 hTSC were seeded onto 6 well plates coated with either 5–10 μg/mL collagen IV (10376931, Fisher Scientific) or 0.5 μg/mL iMatrix 511 (NP892-011 ...
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bioRxiv - Microbiology 2022Quote: ... DF-1 cells were seeded on 6-well plates and transfected with two μg of expression vector using Lipofectamine 2000 (Invitrogen, USA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... and the first/last column of every plate contained final concentrations of 1% DMSO or 50 µM albendazole sulfoxide (Fisher Scientific). 50 μL of the titered L1 culture was added to each well followed by 50 μL of food solution ...
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bioRxiv - Microbiology 2023Quote: ... or 0.5 μl were mixed with 100 μl RPMI + 1% CA + 10 μM EDDHA per well in a 96 well microtiter plate (Falcon flat bottom, Fisher Scientific), respectively ...
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bioRxiv - Bioengineering 2022Quote: ... 150 mM NaCl and were allowed to incubate with the dye for 1 hour at 4 ºC before being loaded into a 384-well qPCR plate (ThermoFisher, BC2384). The plate was then placed into a CFX384 Touch Real-Time PCR Detection System (BioRad ...
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bioRxiv - Developmental Biology 2022Quote: ... containing 1% Matrigel and 3 µM CHIR in a 24-well plate with a super-low cell attachment surface (174930, Thermo Scientific) on an in-incubator orbital shaker ...
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bioRxiv - Developmental Biology 2022Quote: ... the samples were enriched using solid-phase micro-extraction (SOLAμ HRP 10 mg/1 mL 96 well plate, ThermoFisher Scientific, Germany) and reconstituted in 100 μL 5% methanol containing an instrument control standard (ICS ...
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bioRxiv - Microbiology 2024Quote: ... OD600 was adjusted to 1 and 2.5 μl were mixed with 500 μl of RPMI + 1% CA + 10 μM EDDHA per well (final OD600 of 0.005) in a 48 well microtiter plate (Nunc, Thermo Scientific). 200 ng/ml aerobactin (EMC Microcollections GmbH) ...
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bioRxiv - Biochemistry 2024Quote: ... 0.12 x 106 COS-7 cells were seeded into each well of a 6 well plate and transfected with 1 μg total DNA using Lipofectamine p3000 (ThermoFisher Scientific). Cells were rinsed in PBS 5 hours post transfection ...
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bioRxiv - Biochemistry 2023Quote: One hundred thousand UCP1-expressing C2C12 cells were seeded on fibronectin-coated glass coverslips in 12-well plates in 1 ml of DMEM [high glucose with pyruvate (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified antibodies were then neutralized with 14 μl of 1 M Tris-HCl pH 8.0 and buffer exchanged into PBS using Zeba Spin Desalting plates (Thermo Fisher Scientific). Purified antibodies were then stored at 4°C until use.
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bioRxiv - Pathology 2024Quote: ... 2 µl drops of yeast suspension were plated in five 1:5 serial dilutions on 100 mm square plates (Thermo Fisher) with selective SD medium and increasing concentrations (0.07–7 µg/ml ...
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bioRxiv - Immunology 2024Quote: ... The plates were washed one final time with PBST and were then developed by adding 1-step Ultra TMB (Fisher Scientific) for 60 seconds ...
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bioRxiv - Immunology 2023Quote: ... CAR-T cells were then activated for 2 days at 1 × 106 cells per 24-well flat-bottomed plates with an equivalent number of CD3/CD28 washed Dynabeads (Gibco, 11453D), 30U/ml hIL-2 (NIH ...
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bioRxiv - Synthetic Biology 2022Quote: ... HEK293T cells were plated in 5mL DMEM supplemented with 10% FBS and 1% PSG in one well of a 6-well plate (Fisher Scientific) for each validation ...
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bioRxiv - Microbiology 2023Quote: ... OD600 was adjusted to 1 and 2.5 μl were mixed with 500 μl of RPMI + 1% CA + 10 μM EDDHA per well (final OD600 of 0.005) in a 48 well microtiter plate (Nunc, Thermo Scientific) or 0.5 μl were mixed with 100 μl RPMI + 1% CA + 10 μM EDDHA per well in a 96 well microtiter plate (Falcon flat bottom ...
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bioRxiv - Microbiology 2023Quote: ... cells were plated at 650,000 cells per well of a 6 well plate and infected with 6 dilutions from a 10-fold dilution series in 1% FBS (Gibco, MA)/1X non-essential amino acids (Gibco ...
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bioRxiv - Immunology 2022Quote: ... Spike protein in the envelope were incubated with quadruplicates of two-fold serial dilutions of immune sera in 96-well plates prior to infection of Vero E6 cells (1×104 cells / well) in DMEM + 10% FBS (Life Technologies). At 18 hours post infection ...
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bioRxiv - Microbiology 2023Quote: ... The plates were then washed and incubated for 1 hour with horseradish peroxidase (HRP)-conjugated goat anti-human IgM (Invitrogen #A18835), goat anti-human IgA (Jackson #109-036-011) ...
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bioRxiv - Genetics 2023Quote: ... T1 Arabidopsis transgenic plants were selected on plates with MS medium containing 15 mg l−1 hygromycin B (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2023Quote: Purified CHIP (1 μM) or buffer-matched control was immobilized in 96-well plates (Fisher Scientific, non-sterile, clear, flat-bottom) in CHIP buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... Bacmid DNA containing the construct of interest was transfected into 2mL of Sf9 cells at a density of 1×106 cells/mL on a 6-well plate using ExpiFectamine Sf Transfection Reagent (Life Technologies) and following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Splenocytes from OT-I mice were cultured at about 1 × 106 cells/ml in 24 well-plates with RPMI (Thermo Fisher) along with 10% FBS (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... MaxiSorp plates were coated with 1 μg/mL of monoclonal mouse anti-pig IFN-γ antibody (clone P2F6, Thermo Fisher Scientific) in carbonate buffer ...
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bioRxiv - Plant Biology 2023Quote: ... and 40 μL of the resulting supernatant was transferred into the well of a 1 mL 96-well NUNC plate (ThermoFisher Scientific). After adding 10 μL internal mixture (0.5 μg/mL salicylic acid-d4 and 0.5 μg/mL pipecolic acid-d9) ...
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bioRxiv - Cell Biology 2023Quote: ... RAD52KO and RAD52WT cells were seeded at 5 x 104 cells per well in 12-well plates with 1 mL of antibiotic-free media and treated with Lipofectamine RNAiMAX (ThermoFisher #13778075) and 10 pmol of siRNA pools (pool of 4 siRNAs ...
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bioRxiv - Developmental Biology 2022Quote: ... was then transferred to a fresh six-well plate with 3mL of DMEM + 10% BCS + 1% penicillin/streptomycin (Gibco: 15070-063). Two recombinations were performed ...
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bioRxiv - Cell Biology 2023Quote: ... Cas9-transfected U-2 OS cells (1500 cells per well of the 384-well plate) were reverse transfected with crRNAs with 1% (v/v) RNAiMAX (Thermo Fisher) in serum-free media dispensed via a Multidrop combi ...
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bioRxiv - Developmental Biology 2023Quote: Stem cells were grown on culture plates coated with 5micro μg ml-1 recombinant human Vitronectin (rhVTN-N, Life Technologies, #A14700). HESCs were grown in mTeSR1 (StemCell Technologies ...
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bioRxiv - Synthetic Biology 2023Quote: ... 20 uL of cells were diluted in 1 mL of PBS within a clear 96-well microtiter plate and analyzed on the Attune NxT flow cytometer (Thermo Fisher).
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bioRxiv - Neuroscience 2023Quote: ... on 6-well plates pre-coated with Matrigel (1:15 diluted in DMEM/F12 medium; Matrigel: Corning, #356231; DMEM/F12 medium: Gibco, 11320074) at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... in a 96-well plate from 300 nm to 630 nm with 1 nm bandwidth using a Multiskan GO spectrophotometer (Thermo Scientific). An initial protein concentration of 100 μM and an initial volume of 200 μL were used for all spectrophotometric assays ...
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bioRxiv - Plant Biology 2023Quote: ... patens gametophores were grown on KNOP ME agar plates (12 g l−1 purified agar; Oxoid, Thermo Scientific, Waltham, MA, USA). Light intensity in growth cabinets was set to 70-100 µmol photons m−2 s−1 and 16:8 h light/dark cycle at 22 °C ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were seeded with 10 μl of 1×106 cells/ml in a Falcon 24-well cell culture plate (Cat# 353047, Fisher Scientific) and the plate was incubated in the cell-culture incubator (37 °C and 8% CO2 ...
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bioRxiv - Developmental Biology 2024Quote: Total protein was extracted from 1-2 wells of a 6-well plate using RIPA lysis buffer with protease inhibitor (ThermoFisher Scientific). Protein concentration was measured using Pierce BCA kit (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... stock was added to each sample directly before measurement of the melting temperature in a 96-well plate on a QuantStudio 1 Real-Time PCR System reader (Thermo Fisher) with a temperature increase of 0.05 °C/min ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were then diluted 1:1.000 in fresh medium and 200 μl were transferred into 96-well plates (Nunc, Thermo Scientific). OD600 was measured every 15 min for 16 h using a Biotek Synergy HTX plate reader (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... cholerae WT and ΔSI were diluted 1:1000 in the antimicrobial-containing MH media in 96-well plates (Nunc, Thermo Scientific). OD600 was measured every 20 min for 24 h using a Biotek Synergy HTX plate reader (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... cholerae WT and ΔSI were diluted 1:1000 in the antimicrobial-containing MH media in 96-well plates (Nunc, Thermo Scientific). OD600 was measured every 20 min for 24 h using a Biotek Synergy HTX plate reader (Agilent) ...
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bioRxiv - Neuroscience 2023Quote: ... into low passage HEK-293T cells plated at 1×106 cells/well of a poly-D-lysine-coated 6-well plate using Lipofectamine 3000 (Life Technologies). The next day ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 wells of a 6-well plate of mESC (corresponding to 6*10^6 cells approximately) were resuspended in 1 mL Trizol (Invitrogen,15596018) vortexed two times for 30 seconds and incubated 5 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... on confluent fibroblasts was assessed in a 96-well plate format using serial dilutions of 4EGI-1 and the alamarBlue assay as per manufacturer’s instructions (Thermo Fisher Scientific). The efficacy against Toxoplasma was determined using RH strain parasites expressing β-galactosidase in a 96-well format and measuring conversion the metabolism chlorophenol red-beta-D-galactopyranoside (as previously described (41) ...
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bioRxiv - Bioengineering 2023Quote: ... 2 x 107 HEK293T cells were seeded onto 15-cm plates in DMEM supplemented with 10% (v/v) FBS and 1% (v/v) antibiotic-antimycotic (ThermoFisher Scientific). At 16 hr after seeding ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... The reaction was stopped by addition of 10 µL 10% TFA and samples were desalted by stagetipping with C18 filter plugs (Solaµ HRP 2 mg/1 ml 96 well plate, Thermo Scientific). Peptide samples were stored in 40 µL of 0.1% Formic acid at 4 °C until LC-MS analysis.
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded at 100,000 cells per well in 12-well plates 24 h prior to transfecting 1 µg of each plasmid using Lipofectamine 3000 (Thermo Fisher) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... cell pellets corresponding to two wells of a 6-well plate (double-well pellet with same treatment conditions) were lysed in 1% (v/v) TritonX-100 buffer (Fisher Scientific) in DPBS supplemented with 1% (v/v ...
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bioRxiv - Plant Biology 2024Quote: ... 9-day-old seedlings were transferred onto 1% plant agar MS medium plates containing different concentrations of zeocin (0 μM, 10 μM, 50 μM, 170 μM) (Gibco, 10072492). Seedling roots were cut with a razor blade and collected after overnight zeocin exposure ...