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Citations for Thermo Fisher :
4851 - 4900 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: Medium samples from cells and from the engrafted scaffolds were used to measure TGFβ using ELISA (88-8350-22, ThermoFisher), following manufacturer’s guidelines ...
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bioRxiv - Immunology 2020Quote: ... submandibular bleeding was performed to collect blood for measurement of serum IL-1β levels by ELISA (Invitrogen 88-7013-22). At 4 hours ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected and ELISA was performed to detect IL-6 (8 h post stimulation, Cat # 88-7066-22, Invitrogen) and Lcn2 (24 h post stimulation ...
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bioRxiv - Microbiology 2022Quote: ... To measure the protein concentration of IFN-λ1 and -λ2 present in the SN we performed an ELISA following the protocol from the manufacturer: IFN-λ1 (IL-29) from Invitrogen/Thermo Fisher Scientific cat ...
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bioRxiv - Bioengineering 2024Quote: ... the amount of IL-10 in the HDL-rich fraction and the (V)LDL-rich fraction was quantified with an IL-10 ELISA (Invitrogen) and used to calculate the fraction of bound IL-10 ...
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bioRxiv - Systems Biology 2024Quote: ... We detected proteins by chemiluminescence using the SuperSignal® ELISA femto maximum sensitivity substrate (Thermo Fisher Scientific™, Waltham, USA) according to the manufacturer’s instructions and the Amersham™ Imager 600 (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2024Quote: ... Triton- and Guanidine-soluble samples were subjected to analysis using species- and isoform-specific antibodies in Soluble and Insoluble Aβ ELISA (Invitrogen, Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... HMGB1 was measured in cell-free supernantants using an enzyme-linked immunosorbent assay (Tecan HMGB1 ELISA, cat# ST51011, Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The plates were washed 3 times with PBS/Tween 20 0.05% (PBS-T) and saturated for 2 h at room temperature (RT) with 300 µl per well ELISA/ELISPOT diluent 1X (Invitrogen 00-4202-56 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and the protein concentration was determined using the Pierce™ BCA Protein Assay Kit following the manufacturer’s instructions and a Varioskan Lux plate reader (Thermo Fisher Scientific, Waltham, MA, USA). Samples were separated using the Mini-PROTEAN® TGX™ Precast Gel 4-15% (Bio–Rad Laboratories ...
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bioRxiv - Plant Biology 2023Quote: ... Products from the second PCR were purified as described above and concentrations were normalized using SequalPrep Normalization Plate (96) Kit (Thermo Fisher Scientific, Waltham, MA, USA). We pooled and concentrated the libraries using DNA Clean and Concentrator-5 Kit (Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR was conducted in 384-well plates using the SensiFAST SYBR Lo-ROX Kit (Meridian Bioscience) in a QuantStudio 5 (384-well plate) qRT-PCR cycler (Applied Biosystems, Foster City, CA, USA). The qRT-PCR program ran according to enzyme requirements for 40 cycles ...
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bioRxiv - Microbiology 2024Quote: ... qPCR was conducted in 384 well plates using SensiFAST SYBR Lo-ROX Kit (Meridian Bioscience, Cincinnati, OH, USA) in a QuantStudio 5 qPCR cycler (Applied Biosystems, Foster City, CA, USA). The qPCR program ran according to enzyme requirements for 40 cycles ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were washed three times in PBS containing 0.05% Tween-20 and incubated with a secondary fluorescent antibody (all Invitrogen, concentration 1 μg/mL) for 1 hour in the dark at room temperature ...
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bioRxiv - Cell Biology 2020Quote: Fluorescence ratios of pHLARE were first acquired in cell culture medium and then cells were sequentially incubated in a potassium-phosphate buffer (50 mM potassium phosphate, 80 mM potassium chloride, 1 mM magnesium chloride) containing 20 μM nigericin free acid (Thermo Fisher Scientific; N1495) at pH 6.5 and 5.0 ...
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bioRxiv - Microbiology 2019Quote: ... and cultured in Minimum Essential Medium Alpha Modification (MEM-α, HyClone) with 20% fetal bovine serum (FBS, HyClone) and 1% penicillin-streptomycin (P/S, Gibco Life Technologies). Goat adipose-derived mesenchymal stem cells (gASCs ...
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bioRxiv - Developmental Biology 2020Quote: ... for the DMRT1 locus and two single-stranded donor oligonucleotides (ssODNs, 5 pmol of each, see Supplementary Table 1) using Lipofectamine 2000 (Thermo Fisher Scientific, 20). Twenty-four hours after transfection ...
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bioRxiv - Neuroscience 2021Quote: ... stock was made by dissolving 50 µg of dye in 20 µL Pluronic F-127:DMSO (1:4; Life Technologies, Grand Island, NY). Next ...
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bioRxiv - Cell Biology 2019Quote: ... Following washes by 0.1% Tween-20 in TBS the detection of the target proteins was carried out using Super Signal West Femto (Thermo Fisher Scientifics, 34095) and ChemiDoc imaging system (BioRad).
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bioRxiv - Cell Biology 2019Quote: ... Following washes by 0.1% Tween-20-1X TBS the detection of the target proteins was carried out using Super Signal West Femto (Thermo Fisher Scientifics, 34095) and ChemiDoc imaging system (BioRad).
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bioRxiv - Immunology 2019Quote: ... Electroporation was performed at 2150 V 20 ms 1 pulses settings for stimulated PBMC cells using Neon electroporation device (Thermo Fisher, Waltham, MA). Immediately after electroporation ...
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bioRxiv - Genomics 2019Quote: We passed each cell suspension through a 35 µm filter (Falcon) and added 20 µl of 1 µM TO-PRO®-3 Iodide (Thermo Fisher Scientific) per ml to stain dead cells ...
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bioRxiv - Biochemistry 2019Quote: ... cDNA of 1 μLfrom 20 μL reaction volume was amplified by real-time quantitative PCR (Applied Biosystems Quant Studio 3, Thermo Fisher Scientific) with Perfecta SYBR Green Master Fast Mix (VWR ...
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bioRxiv - Bioengineering 2019Quote: ... Hydrogels were subsequently washed in a 0.1% Tween 20 solution in PBS for 20 minutes 4 times and incubated in a 1:500 dilution of goat anti-mouse secondary antibody (Thermo Fisher A-11001) protected from light overnight at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... Slides were blocked in serum-based blocking buffer and incubated with primary antibody against MMP12 (MA5-32011, Invitrogen, 1:50, 20 μg/mL). Slides were washed in PBS (3×5 minutes) ...
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bioRxiv - Neuroscience 2020Quote: ... 20–40 g) and placed on ice in Petri dishes containing 10 mM HEPES (BP310-1, Thermo Fisher Scientific, Waltham, MA, United States) in 1X Hank’s Balanced Salt Solution (SH30588.02 ...
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bioRxiv - Bioengineering 2021Quote: ... Hydrogels were washed 4 × 20 minutes with PBST and then cultured in secondary antibody (Alexafluor 488 goat anti-rabbit; 1:500; Thermo Fisher A-11008) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight at 4 °C with PE-conjugated LAMP1 CD107a clone H4A3 antibody (Cat no. 527993; Molecular Probes; 1:20). After 3× washes ...
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bioRxiv - Cell Biology 2022Quote: ... The culturing media was reintroduced and either the prepared PS-/PC-beads were added at a ratio of 20:1 (Beads: Cells) or apopHEK cells stained with 5 μM eBioscience CFSE (Invitrogen Thermo Fisher Scientific) were added at a ratio of 5:1 (HEK ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed twice in PBS with 0.10% Tween-20 for 3 minutes each and treated with secondary antibodies conjugated with Alexa Fluor 488 (Invitrogen A-11039, 1:200) and/or Alexa Fluor 568 (Invitrogen A-11004 ...
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bioRxiv - Neuroscience 2019Quote: ... then washed in 1x PBS and incubated for 20 min at RT with fluorescent Nissl Stain (NeuroTrace 435/455, Thermo Fisher, 1:150). Afterwards ...
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bioRxiv - Bioengineering 2019Quote: ... microparticles were fabricated by dissolving PLGA (Lakeshore Biomaterials; cat. #5050 DLG4A) at 20 mg/mL in an organic solution containing 9:1 dichloromethane (DCM; Acros Organics, cat. #364230010) to trifluoroethanol (TFE ...
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bioRxiv - Synthetic Biology 2020Quote: ... dry milk and 0.1 % (v/v) Tween 20 and then incubated with mouse anti-6xHis tag monoclonal antibody-HRP conjugate (Clontech or Thermo Fisher Scientific, USA) in the same buffer for 2 h at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... prepared by mixing the stock solution in 0.1% Triton/DPBS at 1/100 and 0.2 mM Hoechst 33342 (ThermoFisher Scientific, stock concentration 20 mM) prepared by mixing the stock solution in 0.1% Triton/DPBS at 1/100 ...
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bioRxiv - Cell Biology 2020Quote: ... Following washes by 0.1% Tween-20-1X TBS the detection of the target proteins was carried out using Super Signal West Femto (Thermo Fisher Scientifics, 34095) and ChemiDoc imaging system (BioRad).
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bioRxiv - Neuroscience 2020Quote: Brain cortex samples were lysed in 1 mL lysis buffer (20 mM HEPES pH 7.9, 1% SDS, and protease inhibitor) with protein concentrations determined by BCA assay (Thermo Fisher Scientific, Cat # 23225). Reduction and alkylation were performed as previously described50 ...
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bioRxiv - Microbiology 2022Quote: ... lysates were resuspended in cleavage assay buffer (20 mM HEPES, 150 mM KoAC, 1 mM DTT) in the presence of protease inhibitors (ThermoFisher, cat. no. 78440). Lysates were incubated with wild type or catalytically inactive poliovirus 3Cpro (C147A ...
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bioRxiv - Microbiology 2022Quote: ... images were manually focused to -2.0um defocus and then 20 1-second frames were collected and drift-corrected using Velox software (Thermo Fisher Scientific, Eindhoven, NL). Final summed images were exported as 16-bit tif files and cropped using ImageJ.
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bioRxiv - Systems Biology 2022Quote: ... organoids from maxi-rings are released from Matrigel by incubating at 37 °C for 20 min in 1 ml/well of 5 mg/mL dispase (Life Technologies #17105-041). Samples are spun at 1000g for 5 min ...
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bioRxiv - Microbiology 2024Quote: ... diluted each to 1 µg/mL in blocking buffer (PBS containing 2% BSA [Sigma-Aldrich], 1% bovine gelatin [Sigma-Aldrich], and 0.05% Tween 20 [Thermo Fisher Scientific]) was added and plates incubated for 2 h at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and quantitative RT-PCR reactions were conducted with 20 ng of template using custom primers (Table 1) and SYBR Select master mix (Applied Biosystems, Waltham, MA) for 50 cycles ...
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bioRxiv - Neuroscience 2024Quote: ... washed with 1X PBS and incubated for 45 min at 20°C with 1 µg/mL of the fluorescent neutral lipid stain Bodipy 493/503 (Life Technologies, California, USA). After washing ...
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bioRxiv - Immunology 2023Quote: Ex vivo isolated and rested CD8+ T cells or transduced T cells from splenocytes were incubated with R10 (RPMI 1640 + 10% FBS + 1% penicillin/streptomycin + L-glutamine + 20 U/ml of IL-2; Gibco, Thermo Fisher Scientific) alone or R10 containing PMA/ionomycin for two hours ...
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bioRxiv - Biochemistry 2023Quote: ... 500 µL of perfusate was dried in 0.5 mL V-vials and reconstituted in 50 μL of 20 mg/mL hydroxylamine hydrochloride (CAS# 5470-11-1; Acros organics, New Jersey) in pyridine (CAS#25104 ...
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bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
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bioRxiv - Genomics 2023Quote: ... Aquarium ASW was supplemented three times per week with one hour of gentle bubbling of compressed carbon dioxide and dosing with 100 mL each of 20 g L-1 potassium nitrate (ThermoFisher, Waltham, MA, USA), 1.5 g L-1 potassium dihydrogen phosphate (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... the electrode area of all HD-MEAs was treated with poly-D-lysine (PDL, 20 μL, 0.1 mg mL-1; A3890401, Gibco, ThermoFisher Scientific, Waltham, USA) for 1 hour at room temperature and then rinsed three times with sterile water ...
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bioRxiv - Genetics 2023Quote: Genomic DNA from cells was isolated using a lysis buffer (10 mM Tris-HCl, 2 % Triton™ X-100, 1 mM EDTA, proteinase K [20 mg/mL]; Thermo Fisher Scientific). Cells were lysed at 60°C for 1 h ...
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bioRxiv - Plant Biology 2023Quote: ... The total RNA samples (1 µg each) were used for DNase I treatment and reverse transcription reactions (20 µl) using SuperScript (Thermo Fisher,Waltham, MA). The resulting cDNA samples (5 of 20 µl each ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed once in 1x PBS with 0.05% Tween- 20 (PBST solution) and then incubated in 1x PBS with a 1:250 dilution of GAR-AF647 (Thermo Fisher Scientific #A32733) secondary antibody for two hours at room temperature with rotation ...