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Citations for Thermo Fisher :
4901 - 4950 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... protein fractions were mixed with SDS-free loading buffer in a 3:1 ratio and loaded on a Novex™ 4–20% Tris-Glycine Gels using NativeMark™ Unstained Protein Standards (Invitrogen™) (range from 20 to 1236 kDa ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein samples were separated by SDS-PAGE ((4-20%; Criterion XT precast gel) with 1× MOPS SDS gel running buffer (NP0001, Invitrogen, Life Technologies Corp). Following electrophoresis ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked in TBST (TBS + 0.1% Tween-20) containing 5% non-fat milk and then incubated with primary TRPV4 antibody (ThermoFisher Scientific PA541066; 1:1000) and GAPDH (Santa Cruz sc-32233 ...
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bioRxiv - Neuroscience 2024Quote: ... mitotic SH-SY5Y cells were differentiated into non-mitotic neuronal cells by the addition of 20 µM all-trans retinoic acid on day 1 (Molecular Probes/Invitrogen, #207340010). From day 1 onwards ...
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bioRxiv - Immunology 2021Quote: ... Secondary antibody was diluted to 1:3000 in blocking buffer and added to the plates for 60 minutes followed by TMB substrate (Thermo Fisher Scientific). Upon stopping the reaction with 1 M H2SO4 ...
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bioRxiv - Microbiology 2019Quote: ... using a Micro Amp Fast Optical 48-well reaction plate with 15 μ L reaction volume containing 1 x Power Sybr Green PCR Master Mix (Thermo Fisher), 0.2 μM of each primer and 1.5 μg of cDNA ...
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bioRxiv - Neuroscience 2022Quote: ... Isolated PBMCs were cultured at a density of 5×105 cells/1 ml CM on 24-well plates coated with Geltrex (Thermo Fisher Scientific). After 24 h of incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... in a 1:9 ratio (in 6-well plates at 60-70% confluency) using Lipofectamine®LTX with Plus™ Reagent (Invitrogen) in Opti-MEM according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: MONHAs were seeded at 1 × 104 cells/cm2 on sterile glass coverslips in 24-well culture plates (Thermo Fisher Scientific, Waltham, MA). After 48 h ...
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bioRxiv - Cell Biology 2019Quote: ... HACAT and KB 3-1 cells were seeded into white 1536-well plates using a Multidrop Combi peristaltic dispenser (ThermoFisher, Waltham, MA) at a density of 250 ...
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bioRxiv - Immunology 2019Quote: ... Embryo carcasses were placed into individual 6 cm2 tissue culture plates containing 1 mL of pre-warmed 37°C TrypLE (Thermo Fisher, 12604013) and were incubated at 37°C for 20 min ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: ... 6.104 HL-1 cells were plated into each well of a 6-well plate and transduced overnight in 1 mL of transduction medium (OptiMEM-GlutaMAX, Life Technologies SAS) containing 5 µg/mL protamine sulfate in the presence of lentivectors (MOI 2) ...
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A key interfacial residue identified with in-cell structure characterization of a class A GPCR dimerbioRxiv - Biochemistry 2019Quote: ... and pcDNA3.1/HA-GPR17 (50 ng) or the mutant GPR17 plasmids (50 ng) per well in 96-well white plate (Thermo Scientific, Denmark). One day after transfection ...
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bioRxiv - Biophysics 2021Quote: Kinetic measurements of human erthyrocyte lysis (1%, v/v in PBS) by all ClyA mutants (at 10μM) was performed using a plate reader (Thermo Fisher - Verioscan reader) at 37◦C in shaking mode (120 RPM) ...
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bioRxiv - Neuroscience 2020Quote: ... The day before transfection, 1× 106 HEK293T cells were seeded in 10cm plate (DMEM medium supplemented with FBS and L-Glutamine, (Gibco, Ottawa, ON)) ...
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bioRxiv - Microbiology 2021Quote: The processed splenocytes were distributed at 1 x 106 per well into Nunc 96-well round bottom microwell plates (Thermo Scientific, UK). The cells were blocked by adding 5µg/ml purified rat anti-mouse CD16/CD32 (mouse BD Fc Block ...
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bioRxiv - Neuroscience 2020Quote: ... and placed in a 6-well plate filled with 1 ml/well of culture media containing 50 % Minimum Essential Medium (MEM, Thermo Fisher Scientific), 23 % EBSS ...
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bioRxiv - Molecular Biology 2020Quote: ... 661W cells were seeded at a density of 1×105 cells/well in 6 well plates (Nunc, Thermo Fisher Scientific, MA, USA) and grown to 80% confluence in growth medium ...
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bioRxiv - Molecular Biology 2020Quote: ... 661W cells were seeded at a density of 1×105 cells/well in 6 well plates (Nunc, Thermo Fisher Scientific, MA, USA) and grown to 80% confluence in growth medium ...
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bioRxiv - Microbiology 2020Quote: ... and THP-1 cells (100 μl of 2 x 105 cells/mL) were seeded in 96 well tissue culture plate (Nunc™, Thermofisher) for 24 hours using DMEM and RPMI media respectively supplemented with 5% FBS in 5% CO2 incubator at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... The plates were then incubated with 100 µL concentration in each well mouse anti-alpaca monoclonal antibody (1:2000; Thermo Fisher Scientific) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: Wild-type HeLa cells were plated in 12-well plates (200’000 cells per well) for 16 hours in 1 ml RPMI (Invitrogen, ref. no. 61870), supplemented with 10 % heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... The transfected plates are rinsed in PBS and incubated in Fluorobrite medium containing NucBlue Hoescht 33342 stain (R37605, Invitrogen, 1 drop/ml). For Arl13b FRAP ...
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bioRxiv - Genomics 2022Quote: ... After 24 h the 4x 5ml input pellets were collected and 1 million cells/replicate were plated on-ADE-URA selection medium in 145 cm2 plates (Nunc, Thermo Scientific). Plates were incubated at 30 C for 7 days inside an incubator ...
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bioRxiv - Bioengineering 2022Quote: ... pools of 10 couples (n=8) were maintained up to 1 month in 6 well plates (9.6 cm2) using 3 mL of M199 (GIBCO, 41150-020, UK), DMEM (GIBCO ...
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bioRxiv - Neuroscience 2021Quote: ... respectively) into 24-well plates and maintained in 500 μl/well complete NDM medium (NDM + 1 μg/ml laminin (Gibco, 23017-015), 1μM cAMP (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: sg-mEX3 or sg-mEX5 and SpCas9-2A-GFP plasmids at 1:1 molar ratio were co-transfected into ~80% confluence OC-k1 cells in 6-well plate using Lipofectamine 3000 (Thermo Fisher Scientific). The cells were incubated for 48 hours and then submitted for fluorescence-activated cell sorting ...
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bioRxiv - Microbiology 2020Quote: ... plates were washed six times with PBS (200 μl) and then blocked for 1-2 hours with Goat Serum (ThermoFisher Scientific, 16210072) at 37°C ...
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bioRxiv - Immunology 2022Quote: ... 3 ×105 Aag2 cells were seeded on an 18 mm, #1 thickness, poly-D-lysine coverslip (Neuvitro, Vancouver, WA) in a 12-well plate (Thermo Fisher Scientific) and cultured at 28°C overnight ...
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bioRxiv - Cell Biology 2022Quote: 10 cm confluent plates of transiently transfected AD293 cells were washed well with PBS and then incubated in 1 mM disuccinimidyl suberate (Thermo Scientific, 21555) in PBS at 37°C for 10 min ...
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bioRxiv - Microbiology 2019Quote: ... procaine (ranging 1 to 8 mg/ml) or dequalinium chloride (ranging 1 to 500 μg/ml) in Flat white 96-well plates with optical bottom (Thermo Scientific Nunc), at a final OD600nm of 0.01 ...
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bioRxiv - Systems Biology 2020Quote: ... Clone cultures were concentrated to 20-fold in M9 buffer and 10 μL was plated onto a well of a 96 well plate containing NGM agar with 2 mM Isopropyl β-d-1-thiogalactopyranoside (IPTG, Fisher Scientific). Plates were dried and stored at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μL DNA sample was added to 14 μL TE diluted PicoGreen in MicroAmp optical 96-well plates (ThermoFisher Scientific, cat #N8010560) as per manufacturer directions ...
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bioRxiv - Bioengineering 2021Quote: ... transfected with 1 μg plasmids at 1050v/20ms/2 pulses and cultured in a 24-well Nunc cell culture plate (Thermo Fisher Scientific) at the density of 200,000/well in PRMI 1640 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: Four to six-days old seedlings were incubated in 24-well cell-culture plates for 1 hour in 50 μM BFA (Invitrogen, Thermo Fisher Scientific) containing liquid growth medium (0.5x MS medium ...
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bioRxiv - Immunology 2019Quote: ... BM cells were plated at a density of 1×106 cells per well in 24-well plates and cultured in 1ml RPMI 1640 medium (Gibco, Paisley, UK) supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: Approximately 2 x 106 cells in 1 mL DMEM10 were seeded into wells of 12-well cell culture plate (Nunc, NY, USA). Two hours later after the cells had adhered ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... formation was induced by culturing cell aggregates in suspension from 1-well of a 6-well plate on a 35×10mm petri dish (Thermo Fisher Scientific) with mTeSR1 medium ...
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bioRxiv - Cell Biology 2022Quote: ... 25 CD45.2+ and 25 CD45.1+/CD45.2+ HSCs were sorted into a 96-well plate with Ham’s F12 media containing final concentrations of 1× Penicillin–streptomycin–glutamine (Gibco cat. # 10378-016), 10 mM HEPES (Gibco cat ...
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bioRxiv - Biochemistry 2022Quote: ... Pellets from six-well plates were resuspended in 50 μl of 1× DNase buffer and incubated with 2 units of DNase (TURBO; Thermo Fisher Scientific) at 37 °C for 1 h ...
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bioRxiv - Biophysics 2022Quote: ... 1 x 106 Sf9 cells were transferred to each well of a six-wells plate and subsequently transfected with 1 μg of bacmid DNA by 6 μl of Cell-fectin (Thermo Fisher Scientific). Baculovirus containing cell supernatants (P1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were quickly washed twice in ice-cold PBS 1x and lysed on the plate by addition of 1 mL of Trizol (Thermo Fisher Scientific). Total RNA was extracted according to manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... Plates were incubated at 4°C overnight and then blocked for 1 hour at 37°C using SuperBlock (ThermoFisher Scientific, Cat. # 37516), and then washed four times with PBS 0.05% Tween-20 (PBST) ...
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bioRxiv - Neuroscience 2024Quote: ... Plates were washed and incubated with 30 μl mouse anti-total-Ub detection antibody (1:500, P4D1, Thermo Fisher, 13-6078-82) for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... Ten L3 per well were cultured in triplicate at 37°C and 5% CO2 for 1 week in 96-well plates for cell culture (Thermo Fisher Scientific) with 200 µL of medium containing 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were diluted in 0.5x PBS + RI and 8 µL loaded into 12 µL of RT-mix in the 48-wells of the SPLiT-seq barcode-1 reverse transcription plate and reverse transcription performed with Maxima H Minus Reverse Transcriptase (Fisher Scientific #EP0753). Cells were then pooled and resuspended into NEB 3.1 Buffer (New England Bio-Labs #B7203S) ...
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bioRxiv - Developmental Biology 2023Quote: ... mESCs were dissociated as mentioned before and seeded at 1.8*104 cells/cm2 density on new 24-well tissue-culture plates (Falcon® 353047) coated with 1% Geltrex (Gibco™ A1413302) in DMEM-F12 (Gibco™ 11320033 ...
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bioRxiv - Microbiology 2024Quote: The sera were prediluted with phosphate buffered saline (PBS) to 1:100 in a polystyrene microtiter plate (Nunc F96 MicroWell flat bottom) and mixed well by shaking ...
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bioRxiv - Cancer Biology 2024Quote: ... CD45.2 BM cells were plated at 1×106 cells/ml in a 10 cm plate in 10 ml of complete StemPro-34 SFM (Thermo Fisher #10639011) containing recombinant mouse cytokines (20 ng/ml IL-3 ...
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bioRxiv - Bioengineering 2023Quote: ... PCs were seeded into 6-well plates coated with collagen IV (100 μg/mL) and were cultured in DMEM (1 g/L glucose, Gibco, cat# 11885084) supplemented with 10% FBS (Sigma-Aldrich ...