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Citations for Thermo Fisher :
4551 - 4600 of 10000+ citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Serum samples from dams were analyzed via enzyme linked immunosorbent assay (ELISA) for IL6 and TNFα (Thermo Fisher Scientific) following manufacturer’s recommendations.
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bioRxiv - Immunology 2022Quote: ... OSP and CT ELISAs were then blocked with warm 200 µl/well of R10 media (RPMI + L-Glutamate (Gibco), 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2019Quote: Serum IL-6 and IL-17A concentrations were assessed by ELISA according to the manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: The concentration of cytokines in the 24h and 48h supernatants from the T57 T cell activation assay was measured by ELISA according to the manufacturer’s instructions (IFNγ: Invitrogen eBioscience, cat#88731477, IL-2: Invitrogen eBioscience ...
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bioRxiv - Immunology 2020Quote: The concentration of cytokines in the 24h and 48h supernatants from the T57 T cell activation assay was measured by ELISA according to the manufacturer’s instructions (IFNγ: Invitrogen eBioscience ...
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bioRxiv - Cell Biology 2021Quote: ... The color reaction was started by adding 100 μL of One-step Ultra TMB ELISA substrate (Thermo Scientific #34028) was added to each well (100 μL) ...
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bioRxiv - Physiology 2021Quote: ... slgA concentrations in saliva were measured using the ready-set-go ELISA system for human IgA (Affymetrix: eBioscience, USA) with a sample dilution of 1:7500 at the University Hospital RWTH Aachen (SI ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected for cytokine profiling by quantitative ELISA for IFNγ and IL-17A as per manufacturer’s instructions (Invitrogen). Prior to flow cytometric analysis for cytokine production ...
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bioRxiv - Pathology 2023Quote: ... CCL2 levels were measured in conditioned media by ELISA (Invitrogen, cat nos. 88-7391-88 and 88-7399-88).
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bioRxiv - Biochemistry 2023Quote: ... followed by the development of signal by treating cells with 200 μl TMB-ELISA (Thermo Scientific, Cat no. 34028) until the light blue colour appeared ...
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bioRxiv - Cell Biology 2023Quote: Human ApoE protein was quantified using the Invitrogen Human ApoE ELISA following the manufacturers guidelines (Invitrogen. Cat no. EHAPOE).
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bioRxiv - Cancer Biology 2021Quote: Feeder-free iPS cell culture was maintained on matrigel coated 6-well plates and routinely passaged with 1 ml accutase or 0.5 mM EDTA (Thermo Fisher Scientific). To enhance single cell survival ...
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bioRxiv - Immunology 2021Quote: ... 1×105 cells per well were aliquoted into round-bottom 96-well plates and washed with 1X PBS (Thermo Fisher Scientific). Washing steps include centrifugation at 500g for 3 min at 4 °C to pellet cells before removal of supernatant ...
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bioRxiv - Developmental Biology 2021Quote: ... on tissue cell culture plates pre-coated with 1:100 dilution of Geltrex Basement Membrane Matrix in DMEM/F-12 (both from ThermoFisher Scientific).
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bioRxiv - Biochemistry 2019Quote: ... DSF was performed on human BACE1 (1 μg) using 96-well qPCR plates (AB Biosystems, UK) with 20x Sypro Orange (Invitrogen, UK) in 50 mM sodium acetate ...
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bioRxiv - Cell Biology 2020Quote: ... Cells seeded on 13 mm coverslips placed in 24 well plates were transfected twice (on day 1 and 2) with Oligofectamine or RNAi MAX (Thermo Fisher) complexed with 20pmol of each indicated siRNA and analyzed 3 to 4 days after the first transfection ...
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bioRxiv - Immunology 2021Quote: ... ROS production upon stimulation with or without 10 μM peptide S100A6-1 coated to a Microfluor® 2 white-bottom 96-well plate (ThermoFisher) was determined by AmplexRed assay [3] ...
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bioRxiv - Biochemistry 2021Quote: The cells were plated at 4 × 104 cells well-1 in a Nunclon Delta-Treated 96-well plate (Thermo Scientific 167008). After allowing an overnight growth for cells to adhere ...
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bioRxiv - Cell Biology 2021Quote: ... with 0.4 µm pore size in 6 well plates with 1 ml Slice Culture Medium as follows: 50% MEM with GlutaMAX (Gibco, 41090036) 18% EBSS ...
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bioRxiv - Microbiology 2021Quote: ... wells of 96 wells plate were coated with 100 µL of 40 µg.mL-1 rat tail type I collagen in sterile PBS (Life Technologies, NY) at 4°C for 18 hours ...
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bioRxiv - Cell Biology 2021Quote: ... or IMS were plated in 6-wells plate (∼300000 cells/well) on day 0 and transfected on day 1 with 6µl of Lipofectamine 2000 (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: DNA transfections were performed by seeding HEK293T cells in 12 well plates at 25% confluency and adding 1 μg of each DNA construct and 4 μL of Lipofectamine 2000 (Thermo Fisher). RNA transfections were performed by adding 1 μg of each RNA construct and 3.5 μL of Lipofectamine MessengerMax (ThermoFisher) ...
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bioRxiv - Genomics 2022Quote: ... After 24 h the 4x 5ml input pellets were collected and 1 million cells/replicate were plated on-ADE-URA selection medium in 145 cm2 plates (Nunc, Thermo Scientific). Plates were incubated at 30 C for 7 days inside an incubator ...
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bioRxiv - Immunology 2022Quote: ... the cells were washed and plated at 1 million cells per well in a V-bottom 96 well plate in methionine-free R10 (Gibco #A1451701) supplemented with 10% FBS ...
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bioRxiv - Plant Biology 2022Quote: ... was used for floral dip transformation of pTAN1:CFP-TAN1 and selected on 1/2 MS plates containing 100 μg/mL gentamicin (Fisher Scientific). T1 seedlings were screened for mScarlet and CFP signal and then transferred to soil to self.
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bioRxiv - Cancer Biology 2022Quote: ... and cultured with PDAC tumor organoids in the lower compartment of the 24-well plate in DMEM containing 5% FBS and 1% penicillin/streptomycin (Thermo Fisher). For IL1α neutralization experiments ...
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bioRxiv - Immunology 2021Quote: ... yoelii AMA-1 or MSP-119 proteins in sodium bicarbonate buffer were used to coat high binding Immunlon HBX plates (Thermo Scientific) at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: Adenovirus containing the MCMV IE1 gene was generated by transfecting purified pAd5-MCMV_IE1 into 293A cells in 1 well of a 6 well plate using Lipofectamine 3000 (ThermoFisher, Waltham, MA) according to manufacturer’s instruction ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and fly incubation buffers were de-salted using a 96-well HyperSep C8 solid phase extraction (SPE) plate with 1 mL well volume and 100 mg bed weight (Thermo Scientific). The SPE plates were coupled with a vacuum manifold and vacuum pump ...
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bioRxiv - Genetics 2019Quote: ... qRT-PCR was performed with the cDNA template diluted 1:4 with nuclease-free water then pipetted into quadruplicate wells of a 384 well optical plate (Cat#4309849 Applied Biosystems). Thermo Maxima SYBR Green/Rox qPCR Master Mix 2X (Cat#K0221 ...
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bioRxiv - Cancer Biology 2019Quote: ... VM-CUB1 and RT-112 cells were seeded at 1 × 105 cells per well in 6-well plates along with siRNA duplexes or miRNAs using Lipofectamine RNAiMAX Reagent (Life Technologies). At 72 hours after transfection ...
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bioRxiv - Immunology 2019Quote: ... or 1×105 (96-well plate) naïve CD8+ T-cells were activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher, 11132D) in a 1:1 beat to T-cell ratio and cultured in 1 ml (48WP ...
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bioRxiv - Synthetic Biology 2019Quote: ... of each starting culture was diluted 1:50 in fresh M9c medium (100 µL) in a Nunc™ Edge 2.0 96-Well Plates (Thermo Fisher). These cultures were grown in the presence and absence of aTc (200 ng/mL ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was diluted 1:5 in nuclease-free water and qPCR reactions were performed in MicroAmp Fast Optical 96-Well Reaction Plates (Applied Biosystems) using PrimeTime qPCR assays (IDT ...
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bioRxiv - Microbiology 2019Quote: ... or dequalinium chloride (ranging 1 to 500 μg/ml) in Flat white 96-well plates with optical bottom (Thermo Scientific Nunc), at a final OD600nm of 0.01 ...
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bioRxiv - Systems Biology 2020Quote: 1×10^6 cells were seeded in wells of 6-well plates (FALCON # 353046) in MEM without phenol red (Gibco # 51200038) supplemented with 0.5% Fetal Calf Serum (VWR #89510-184) ...
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bioRxiv - Systems Biology 2020Quote: 1×10^6 cells were seeded in wells of 6-well plates (FALCON # 353046) in MEM without phenol red (Gibco # 51200038) supplemented with 0.5% Fetal Calf Serum (VWR #89510-184) ...
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bioRxiv - Systems Biology 2020Quote: 1×10^6 cells were seeded in wells of 6-well plates (FALCON # 353046) in MEM without phenol red (Gibco # 51200038) supplemented with 0.5% Fetal Calf Serum (VWR #89510-184) ...
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bioRxiv - Immunology 2020Quote: 1 μg of IgG1 (rituximab in PBS, 50 μl/well) per well was incubated on a 96well microtiter plate (NUNC Maxisorp) pre-treated (2h at RT ...
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bioRxiv - Microbiology 2021Quote: ... Aclar were washed 3 times in fresh KPBS and transferred to a 48-well plate (Costar 3548) with 1 mL 10% buffered formalin (Fisher Scientific) with gentle agitation and shielded from light for 12-16 hours ...
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bioRxiv - Genetics 2020Quote: 3×105 HeLa cells were seeded to each well of 6-well plates and cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Life Technologies) 24 hours before transfection ...
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bioRxiv - Genetics 2020Quote: ... cells were seeded on 48-well plates and transfected with Cas9 plasmid (300 ng) and gRNA plasmid (200 ng) using 1 ul of Lipofectamine 2000 (Life Technologies) in Opti-MEM according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Individual embryos were placed at the center of 15-cm plates and incubated for 45 min in 1 mL trypsin-EDTA 0.25% (Life Technologies 25200072). Excess trypsin was carefully aspirated ...
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bioRxiv - Bioengineering 2021Quote: ... 1:1000 mouse anti-his capture antibody in PBS was coated onto Immulon 2 HB 96-well plates (Thermo Fisher Scientific) at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... or 1×105 (96-well plate) naïve CD8+ T cells were activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher, 11132D) in a 1:1 bead to T cell ratio and cultured in 1 ml (48-well plate ...
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bioRxiv - Microbiology 2020Quote: ... and THP-1 cells (100 μl of 2 x 105 cells/mL) were seeded in 96 well tissue culture plate (Nunc™, Thermofisher) for 24 hours using DMEM and RPMI media respectively supplemented with 5% FBS in 5% CO2 incubator at 37°C ...
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bioRxiv - Immunology 2021Quote: ... three times and plates were incubated with secondary goat anti-mouse Alexa-Fluor-546-labeled antibody at 1:1,000 dilution (Thermo Fisher Scientific) for 2 h at room temperature in the dark ...
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bioRxiv - Immunology 2021Quote: ... DCs were then added to 96-well plates pre-cultured with bEnd.3 cells or embedded in bovine collagen I (1 mg/ml, Life Technologies). Live cell imaging was performed for 1 h ...
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bioRxiv - Immunology 2020Quote: ... Purified B cells were cultured in 24 well plates (TPP, Trasadingen, Switzerland) at 106 cells/well in 1 ml RPMI 1640 complete medium (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Secondary antibody was diluted to 1:3000 in blocking buffer and added to the plates followed by TMB substrate (Thermofisher Scientific). Upon stopping the reaction with 1M H2SO4 ...