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Citations for Thermo Fisher :
4251 - 4300 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cloning vector pKNG101 plasmid was also amplified using Phusion Master Mix (Thermo Scientific). Afterwards ...
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bioRxiv - Microbiology 2023Quote: ... After mixing the plasmids in 200 μL of Opti-MEM (ThermoFisher, Woolwich, UK), Fugene HD (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... Capped sense RNA was synthesized from linearized plasmids using mMessage mMachine kit (Invitrogen) and RNA was purified using RNeasy Mini kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: All expression and rescue plasmids were generated via the multisite gateway system (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... and terminator part plasmids into a kanamycin-resistant entry vector using Esp3I (Thermofisher) (level 2) ...
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bioRxiv - Developmental Biology 2023Quote: ... Amplicons were introduced to pDONR221 entry plasmids using BP clonase (11789100, ThermoFisher Scientific), and subsequently to destination plasmids using LR clonase (11791020 ...
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bioRxiv - Immunology 2023Quote: ... Plasmids were transiently co-transfected in FreeStyle 293-F cells using 293Fectin (ThermoFisher). All cells were tested monthly for mycoplasma ...
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bioRxiv - Microbiology 2023Quote: ... and cloned in the plasmid pCR2.1 using the TOPO-TA CloningTM Kit (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... cDNA plasmids clones were transfected in mESCs with Lipofectamine LTX (Thermo Fisher Scientific) according to the manufacturer instructions.
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bioRxiv - Biochemistry 2022Quote: The EN WT plasmid was transformed into BL21 Star (DE3) competent cells (Invitrogen) for large-scale expression ...
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Docking Domain Engineering in a Modular Polyketide Synthase and its Impact on Structure and FunctionbioRxiv - Biochemistry 2023Quote: ... the GeneJET Plasmid Miniprep Kit and the GeneJET Gel Extraction Kit from ThermoFisher Scientific were used ...
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bioRxiv - Cell Biology 2023Quote: ... DNAJB1H32Q,D34N-HA in pcDNA5/FRT/TO mammalian expression plasmid (Thermo Fisher Scientific).69 Gene sequences were verified by sanger sequencing.
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bioRxiv - Developmental Biology 2023Quote: ... gRNAs were synthesized from plasmids using a MEGAshortscript T7 Transcription Kit (Invitrogen AM1354) and purified.
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bioRxiv - Immunology 2023Quote: ... Spike encoding plasmids were diluted in cold OptiPRO SFM (Life Technologies, 12309-050), mixed with ExpiFectamine CHO Reagent (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Transfection with plasmid DNA and siRNA was carried out using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2023Quote: ... Cells co-expressing two plasmids were transiently transfected with Lipofectamine 2000 (Invitrogen #11668019) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The plasmid expressing the PhiC31 integrase was transfected using Lipofectamine 3000 (Life Technologies), in combination with the Zdhhc20 plasmid containing a PGK promoter ...
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bioRxiv - Cell Biology 2023Quote: ... and pMD2G plasmids were transfected into HEK293-T cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... or G32R null biosensor plasmids using Lipofecatime 3000 or 293fectin (Thermo Fisher Scientific). Flow cytometry was used to select and enrich for the population of cells expressing respective biosensors ...
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bioRxiv - Developmental Biology 2023Quote: ... The promoter fragment was successfully cloned into an existing pJet1.2 plasmid backbone (ThermoFisher) containing an mOrange2 (mOr2 ...
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bioRxiv - Cell Biology 2023Quote: ... Subcloned plasmids were used to transform into Expi293F cells (Thermo Fisher Scientific, # A14527) using the Expi293 expression system (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Plasmids DNA repair templates were designed using a pMK (Life Technologies, Carlsbad, CA) vector backbone ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293FT cells were transfected with a plasmid encoding GFP-GPI using Turbofect (Invitrogen) while seeding and according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Ligated plasmid products were introduced by heat shock transformation into competent Stbl3 (Invitrogen). The cloning products were confirmed using Sanger sequencing ...
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bioRxiv - Developmental Biology 2023Quote: ... A plasmid was constructed utilizing the Gateway LR Clonease II Plus system (ThermoFisher). To create uas:cas9mkate-u6:piezo1gRNA ...
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bioRxiv - Cell Biology 2023Quote: Equal amounts of plasmid were transfected into the cells using Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... 0.5µg Plasmid DNA and 2µM siRNA were diluted in Optimem (3198-062, Gibco) and mixed ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cells were transfected with plasmids using Lipofectamine 3000 (Invitrogen cat no. 3000008) according to the manufacturer’s instructions with a slight modification ...
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bioRxiv - Genetics 2023Quote: ... Each enhancer candidate was TOPO-cloned into the pENTR5′-TOPO plasmid (K59120, Invitrogen) using halt-volume reactions according to the manufacturer’s instructions (half-volume reactions) ...
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bioRxiv - Developmental Biology 2023Quote: ... Assembled Cas9/sgRNA plasmids were transfected into XEN cells using Lipofectamine 3000 (Invitrogen). E2-Crimson-positive cells were assessed for successful knockout of gene expression and reprogramming potential (see Figure 5B-C and S6C) ...
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bioRxiv - Cell Biology 2023Quote: ... the plasmids were transfected into the cells using Opti-MEM (Thermo Fisher Scientific) and Viofectin Transfection Reagent (Viogene ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were transfected when needed with 15 μg plasmid using Lipofectamine 2000 (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: ... and the linear plasmid was ligated using T4 DNA ligase (Thermo Scientific™) following manufacturer protocols ...
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bioRxiv - Microbiology 2023Quote: ... A pCR 4-TOPO or pCR- XL-2-TOPO plasmid (ThermoFisher, Waltham, Massachusetts) with an insert spanning ITS1-5.8S rRNA-ITS2-D1/D2 region of 28S rRNA from a pure culture strain was used as a positive control ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were cotransfected at DIV14 with plasmids by using Lipofectamine 2000 reagent (Invitrogen). Cells were fixed at DIV21 with 4% (vol/vol ...
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bioRxiv - Microbiology 2023Quote: ... the cloning vector pKNG101 plasmid was amplified using Phusion Master Mix (Thermo Scientific) and digested by DpnI (NEB BioEngland ...
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bioRxiv - Plant Biology 2023Quote: ... The obtained plasmids were recombined by an LR clonase II (Thermo Fisher Scientific) into the pXCSG-mYFP vector with a C-terminally fused mYFP epitope tag ...
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bioRxiv - Microbiology 2023Quote: ... 62–64 by transient plasmid cotransfection in the Expi293 Expression System (Thermo Fisher) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: Plasmid pBH-vsx2-memCerulean-pA was made using the Gateway system (ThermoFisher, 12538120) with combinations of entry and destination plasmids as follows ...
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bioRxiv - Biophysics 2023Quote: ... cells were transfected with 500 ng of each plasmid via lipofectamine 2000 (Invitrogen) according to manufacturer’s protocol.
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The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretionbioRxiv - Neuroscience 2024Quote: All expression plasmids were generated using the Tol2kit (63) and Gateway system (Invitrogen). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... transfections were performed with purified plasmids using lipofectamine 3000 (Invitrogen, Waltham, MA, USA) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... The plasmid was transfected into cells using lipofectamine 2000 and Plus reagent (Invitrogen) and incubated for 6 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 1-2 µg plasmid DNA were added to 50 µl OptiMEM (Thermo Fisher) and another 50 µl PEI mix (OptiMEM with double the amount of PEI than DNA ...
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bioRxiv - Bioengineering 2023Quote: ... the lentiviral plasmids were added to the cells with Lipofectamine 2000 (ThermoFisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... and 1 µg of plasmid per well in Opti-MEM (Thermo Fisher Scientific), supplemented with 1 µM of all-trans-retinal (Sigma) ...
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bioRxiv - Bioengineering 2023Quote: ... and the mini-donor plasmid were combined with Lipofectamine Stem Transfection Reagent (ThermoFisher) (1:4 ratio ...
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bioRxiv - Biochemistry 2023Quote: ... plasmids were transfected into NIH-3T3 cells using Lipofectamine 3000 reagent (L3000015, Invitrogen). The final concentration of plasmids was 1 μg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... coli was purified using the GenJET Plasmid Miniprep kit (Thermo Scientific, Waltham, USA). Chromosomal DNA from Hm ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfection of plasmids and siRNA was performed using Lipofectamine LTX (Thermo Fisher Scientific) and Lipofectamine RNAiMAX (Thermo Fisher Scientific) ...