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Citations for Thermo Fisher :
4201 - 4250 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... equal amounts of the 4 plasmid DNAs were transfected into Expi293 cells (ThermoFisher) using Expi293 transfection reagent (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids and RLuc-RNA reporters were transfected using the Lipofectamine 3000 (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... were transfected with a plasmid solution containing 1 ml of Opti-MEMTM (Gibco), 60 µl of 1mg ml-1 polyethylenimine (PEI ...
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bioRxiv - Cancer Biology 2024Quote: ... completed to 500 ng total DNA with pcDNA3.1 plasmid (V79020, Thermo Fisher Scientific) using CalFectinTM Cell Transfection Reagent (SL100478 ...
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bioRxiv - Microbiology 2024Quote: ... All plasmids were sequenced using a 3130XL or 3500XL Genetic Analyzer (Applied Biosystems) to confirm introduced mutations.
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bioRxiv - Cell Biology 2024Quote: ... pCS2-based expression plasmids were transfected into HeLaK cells with Lipofectamine 2000 (Invitrogen) according to manufacturer’s protocol and into Hek293T cells using a calcium phosphate-based method ...
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bioRxiv - Developmental Biology 2023Quote: ... the HEK293T cells were transfected with indicated plasmids using Lipofectamine 2000 (Life Technologies). After 48 h ...
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bioRxiv - Microbiology 2024Quote: ... coli strains were performed using the PureLink™ Quick Plasmid Miniprep Kit (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... was co-transfected with an L1 promoter reporter plasmid using Lipofectamine 3000 (Invitrogen). The target sequences for mouse Yy1-specific siRNAs are ...
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bioRxiv - Molecular Biology 2024Quote: ... The DSS1 gene was subcloned into a modified pcDNA5/FRT/TO plasmid (Invitrogen), resulting in an N-terminal 2x Strep and 3x FLAG tag ...
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bioRxiv - Pathology 2024Quote: ... and β-galactosidase (β-gal) plasmid using Lipofectamine 3000 Reagent (L3000015, Thermo Fisher). A total of 1200 ng of plasmid DNA was consistently transfected ...
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bioRxiv - Neuroscience 2024Quote: ... Linearised plasmid was transcribed to cRNA using the T7 mMessage mMachine kit (Ambion) and purified using RNeasy Mini Kit (QIAGEN) ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid concentration was measured using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific), and 20 μg of the plasmid was digested with the restriction enzyme PacI ...
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Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availabilitybioRxiv - Cell Biology 2023Quote: ... HEK293 Phoenix cells were transfected with the plasmid using Lipofectamine 3000 (ThermoFisher Scientific). The transfected cells were incubated at 37°C for 5 hr ...
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bioRxiv - Genetics 2024Quote: The following plasmids were purchased: pLenti6.2/V5-DEST (Thermo Fisher Scientific Cat. # V36820); pENTR2B (Thermo Fisher Scientific Cat ...
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bioRxiv - Evolutionary Biology 2024Quote: ... with plasmids expressing wildtype (WT) or mutant TRPM2 channel and pEGFP-N1 (Invitrogen) that expresses enhanced green fluorescent protein (GFP ...
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bioRxiv - Cell Biology 2024Quote: Full-length and truncated TFG-encoding plasmids were transfected into Expi293F cells (ThermoFisher) at 1 µg/ml culture using Expifectamine 293 Reagent per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... NES-GFP-NES-GFP-CL1 and GFPnonstop plasmids using Lipofectamine 3000 (Life Technologies) respectively ...
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bioRxiv - Immunology 2024Quote: ... and cells were transfected with DNA plasmids was performed using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The indicated plasmids were transfected into cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... DNA was extracted using the GeneJET Plasmid Maxiprep Kit (Thermo Fisher Scientific, K0492).
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bioRxiv - Immunology 2024Quote: ... mixed with the relevant plasmid and subsequent culturing under selection with G418 (Gibco) supplemented EX-CELL 420 serum-free media (Merck).
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bioRxiv - Immunology 2024Quote: ... The *192C*24 and K183S/K184S/*192C*24 plasmids were produced by ThermoFisher. The pmaxGFP vector was provided by Lonza ...
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bioRxiv - Biophysics 2024Quote: ... All plasmids were separately co-transfected into HEK293T cells using lipofectamine 2000 (Invitrogen). 100,000 cells were plated per well in media (10% FBS ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µg of plasmid was transfected via lipofection using Lipofectamine 2000 reagent (ThermoFisher), in medium free of antibiotics and antifungals ...
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bioRxiv - Cell Biology 2024Quote: ... The plasmids were co-transfected into HeLa cells with LTX transfection reagent (Invitrogen). 5-7 days after transfection cells were fixed and immunostained with required antibodies.
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bioRxiv - Biochemistry 2024Quote: ... the cells were transfected with pcDNA3.1(+) or SIDT1 plasmids using Lipofectamine 3000 (Invitrogen). After 24 hours ...
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bioRxiv - Synthetic Biology 2023Quote: ... run on 1% agarose gels and recovered using GeneJET Plasmid miniprep kit (ThermoFisher). Recovered fragments were assembled using NEBuilder kit (NEB) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmids were cloned by Gibson isothermal assembly and propagated in DB3.1 cells (Invitrogen). We also generated a series of constitutive lentiviral plasmids as part of this work pLV-EgI-BlastR (EF1a-Gateway-IRES-BlastR) ...
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bioRxiv - Cell Biology 2023Quote: Stable lines were obtained by cotransfecting the plasmid of interest with pCoHygro (Invitrogen) or pCoPuro (Addgene plasmid #17533 ...
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bioRxiv - Microbiology 2023Quote: ... The amplified linear plasmid backbone was DpnI-digested with Fastdigest DpnI (Thermo Fisher) to remove residual template DNA prior to the SLiCE reaction ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid was transfected using Lipofectamine™ 2000 Transfection Reagent (Invitrogen™, 11668019) at a 1:1 µg DNA to µL Lipofectamine™ ratio ...
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bioRxiv - Biophysics 2024Quote: ... The mixture of expression plasmids was transfected using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... The mixture of expression plasmids was transfected using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids (3ug per well) were transfected into HEK293 cells using lipofectamine 2000 (Invitrogen), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR fragment was integrated into the pcDNA5/FRT/TO plasmid (Thermo Fisher) by InFusion Snap assembly (Takara) ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg of each plasmid was mixed with OPTI-MEM (Thermo Fisher Scientific) containing 10 µL of PEI reagent (2 mg/mL concentration of PEI MAX MW 40,000 ...
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bioRxiv - Immunology 2023Quote: ... and pRL-TK Renilla plasmid using lipofectamine 3000 reagent (Thermo Fisher, USA, No.L3000015). The concentrations of PD1 promoter region and control vector were optimized at same level before the transfection into HEK293T cells ...
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bioRxiv - Genomics 2022Quote: ... Recombinant plasmids were transfected into cell lines using Lipofectamine 2000 (Invitrogen; cat.no: 11668019). Vector backbone and unmethylated constructs were used as internal controls for comparison ...
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bioRxiv - Genetics 2023Quote: Purified PCR products or plasmids were sequenced by BigDye Terminator 3.1 (Applied Biosystems). Primers used for sequencing are listed in Table S1 ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid constructs were transiently co-transfected to COS7 cells using Lipofectamine2000 (Invitrogen) approximately 24 hours before the data acquisition ...
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bioRxiv - Neuroscience 2023Quote: Base-editor plasmids were codon optimized and synthesized by GeneArt (Thermo Fisher Scientific). The base editor was intein-split to two halves in the SpCas9 sequence at amino acid positions 310 ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid DNA or siRNA of phosphatases was transfected using Lipofectamine 2000 (Invitrogen; 52887) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and the respective construct plasmid using TurboFect (Thermo Fisher Scientific, Waltham, MA, USA). 48h post transfection ...
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bioRxiv - Cell Biology 2023Quote: ... Lentivirus was created by transduction of each plasmid with Lipofectamine 3000 (Invitrogen, L3000075) into HEK293T cells ...
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bioRxiv - Cell Biology 2023Quote: ... MCF-7 cells were transfected with MitoDendra2 plasmid DNA using Lipofectamine 2000 (Invitrogen). Selection pressure was provided by medium (DMEM-GlutaMax with 10% FBS ...
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bioRxiv - Biophysics 2023Quote: ... The plasmid (1.0 µg/dish) was transfected using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected 24 h later with plasmid DNA with Lipofectamine 2000 (Invitrogen). HEK cells were fixed in 4% PFA 24 h after transfection and washed three times with PBS ...
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bioRxiv - Molecular Biology 2023Quote: Transfection of expression plasmids (Supplemental Table S1) was performed with Lipofectamine 2000 (Invitrogen) and Opti-MEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... pfnr and parcA were purified (GeneJET plasmid miniprep kit, Thermo Fisher Scientific, Lithuania) and confirmed by visualization of insert release following restriction digestion and Sanger sequencing (Aggrigenome ...