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Citations for Thermo Fisher :
4151 - 4200 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... and transfected with plasmid DNA/ lipofectamine complexes (Lipofectamine LTX (Life Technologies, Carlsbad, CA), 2.5 ug total DNA ...
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bioRxiv - Synthetic Biology 2021Quote: ... DNA was extracted following standard maxiprep plasmid extraction protocol (ThermoFisher, GeneJet maxiprep kit) using 2-4 g of pooled colonies instead of liquid culture ...
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bioRxiv - Biochemistry 2021Quote: ... 3.75 µg psPAX and 1.5 µg spike containing plasmid using lipofectamine 3000 (Thermofisher). 4 x 106 cells were plated 16-24 h prior to transfection ...
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bioRxiv - Cell Biology 2019Quote: ... All plasmid sequences were verified using Sanger sequencing (3100 Genetic Analyzer, Applied Biosystems).
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bioRxiv - Genomics 2021Quote: ... and 40ng of PUF9R-iRFP670 fusion plasmid using 1.5 μl Lipofectamine LTX (Invitrogen). For competitor experiments ...
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bioRxiv - Cell Biology 2020Quote: ... The Entry plasmids were then recombined using the Gateway recombination cloning system (Invitrogen) into pPTAPmChW attB (described in Hudson & Cooley ...
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bioRxiv - Cell Biology 2020Quote: ... PShuttle-CMV plasmids were then digested overnight with MssI (Thermo Fisher Scientific, IVGN0244) and purified using E.N.Z.A.® Gel Extraction Kit (Omega Bio-Tek ...
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bioRxiv - Cell Biology 2020Quote: ... Viruses were made in HEK293T cells from a pLenti6/V5-DEST plasmid (Invitrogen) containing mCherry-H2B (gift from Torsten Wittmann ...
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bioRxiv - Microbiology 2021Quote: Cells were transfected with 2µg of indicated plasmid DNA using Lipofectamine 2000 (Invitrogen) following manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... the plasmids were transfected into ExpiCHO cells (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s recommendations and the supernatants were collected 6 days post-transfection ...
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bioRxiv - Microbiology 2021Quote: Lentivirus were produced in HEK293T cells transfected with the following plasmids (ThermoFisher, OHS4735): 6µg pLEX-MCS-1::NA-GFP WT/E229A ...
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bioRxiv - Microbiology 2021Quote: ... Transfection of oligonucleotides and/or plasmids were performed using Lipofectamine™ 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... plasmids and PEI were diluted with Opti-MEM media (Gibco, Gaithersburg, MD, USA) and incubated with HEK 293T cells ...
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bioRxiv - Microbiology 2020Quote: ... An L5attP-zeoR-cydABDC-operon complementing plasmid was assembled by Gateway reaction (Invitrogen) and transformed into the hygR cydA mutant to generate the cydABDC-complementing strain ...
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bioRxiv - Immunology 2020Quote: ... Genes of interest were subcloned into pcDNA3.1 Zeocin expression plasmids (Invitrogen V860-20) with an elongated CMV promoter using HindIII/XhoI restriction sites ...
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bioRxiv - Immunology 2020Quote: ... and N SARS-CoV-2 proteins were cloned into pVAX1 plasmid (Thermo Fisher), i.e. ...
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bioRxiv - Microbiology 2021Quote: Plasmids expressing the crRNA with flanking repeats were ordered (GeneArt, Thermo Fisher Scientific) as pMK-RQ-0582anti#1-anti#3 ...
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bioRxiv - Immunology 2020Quote: Plasmid DNA repair templates were designed using a pMK (Life Technologies, Carlsbad, CA) vector backbone ...
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bioRxiv - Immunology 2020Quote: ... 300 μg of the PB-CMV plasmid DNA and 400 μL 293fectin (ThermoFisher) were each added to separate tubes containing 15 mL of Opti-MEM medium (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... The plasmids were transiently or stably transfected into cells using Lipofectamine 2000 (Invitrogen).
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bioRxiv - Immunology 2020Quote: ... plasmid DNA (pVAX1-SARS-CoV-2 Spike) was transfected with Lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... were stably transfected with the BRE reporter plasmid using Lipofectamine 3000 (Invitrogen #L300015) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: C2C12 cells were transfected with plasmid pCMV6-ALK2R206H using Lipofectamine 3000 (Invitrogen #L300015) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... TMEM24 CRISPR/Cas9 plasmids were transfected into MIN6 by Lipofectamine 3000 (ThermoFisher, L3000015). After 24-hours ...
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bioRxiv - Genetics 2020Quote: ... Cells were transfected with 6 μg of each plasmid using Lipofectamine 2000 (Invitrogen), harvested 24 hours after transfection ...
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bioRxiv - Immunology 2021Quote: Plasmids were transfected to HeLa cells or HEK293T cells using Lipofectamine 2000 (Invitrogen) or PEI max (Polysciences ...
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bioRxiv - Microbiology 2021Quote: ... The plasmids were transfected into HEK Expi293F cells (Thermo Fisher Scientific, Cat# A14527) using polyethylenimine (PEI ...
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bioRxiv - Microbiology 2022Quote: ... The overlap extension product was cloned into plasmid pCR8/GW/TOPO (Life Technologies) following manufacturers instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... plasmids pCDF and pCOLA were cleaved with NheI and Eco81I (ThermoFisher cat#FD0374) and isolated using a runView electrophoresis system (Cleaver Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... cells were transfected with the respective plasmids using Lipofectamine 3000 reagent (Invitrogen, L3000008) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... putida cells with selected plasmids using 2 mm gap cuvette (Thermo Fisher Scientific) and GenePulser XcellTM (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... and transiently plasmid transfected using Lipofectamine® 3000 (Cat. no. L3000-015, Invitrogen) for 24h ...
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bioRxiv - Biophysics 2022Quote: ... 200 ng of DNA plasmid and 0.6 μL Lipofectamine 2000 reagent (ThermoFisher Scientific) were mixed into 60 μL OptiMEM (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and then transfected into HEK293T cells with packaging plasmids (psPAX2 and pMD2G, Invitrogen). The viral supernatant was collected after 48 hours and 72 hours and passed through a 0.45 µm filter ...
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bioRxiv - Immunology 2022Quote: ... coli cells containing a baculovirus shuttle vector and helper plasmid (DH10Bac cells; ThermoFisher) to obtain colonies containing recombinant bacmids ...
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bioRxiv - Microbiology 2022Quote: ... was used for plasmid propagation and was cultivated in LB Broth (ThermoFisher: 12795027), with the addition of 100 μg.mL−1 ampicillin or 50 μg.mL−1 kanamycin for selection.
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bioRxiv - Microbiology 2022Quote: ... Spike encoding plasmids were diluted in cold OptiPRO SFM (Life Technologies, 12309-050), mixed with ExpiFectamine CHO Reagent (Life Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... then transferred into expression plasmids by LR reactions (Invitrogen, Catalog Number 11791-020). The constructions for protein expression in Escherichia coli were ligated coding sequence into pETnT by in-fusion [22] ...
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bioRxiv - Immunology 2022Quote: ... 350 μg plasmids in 15ml Opti-MEM™ (Thermo Fisher Scientific cat.# 31985070) were filtered and mixed with 1.8 ml 40K PEI (1mg/ml ...
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bioRxiv - Microbiology 2022Quote: ... AGS cells were transiently transfected with tf-LC3B plasmid using Lipofectamine 2000 (Invitrogen). 48 h after transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... the ColE1 replication origin was first amplified from plasmid pTZ57R (Thermo Fisher Scientific), appended with the SmaI restriction site at one end ...
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bioRxiv - Molecular Biology 2022Quote: ... the emdyp reading frame was cloned into expression plasmid Pet151 D (Thermo Fisher) using the AQUA cloning procedure as described by Beyer et al ...
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bioRxiv - Neuroscience 2022Quote: ... All plasmid sequences were verified using Sanger sequencing (3100 Genetic Analyzer, Applied Biosystems).
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bioRxiv - Plant Biology 2022Quote: ... The genes/fragments were then cloned into a pDonr207 plasmid (Thermo Fisher Scientific) for future use.
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bioRxiv - Genetics 2022Quote: ... The digested plasmids were analyzed with 0.7% gel electrophoresis with SYBR-gold (Invitrogen) staining ...
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bioRxiv - Plant Biology 2022Quote: ... and finally recombined into the pKGWFS7 destiny plasmid using the Gateway® (Invitrogen) system.
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bioRxiv - Microbiology 2023Quote: ... coli strains were performed using the PureLink™ Quick Plasmid Miniprep Kit (Invitrogen). Hfx ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal neuronal cultures were transfected with plasmid cDNA using Lipofectamine2000 (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids were transfected into HEK293A cells using Lipofectamine 2000 (Life Technologies, CA, USA). Transfected cells were grown from single-cell clones and selected with G418 (2 mg/mL) ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were introduced by transfection with 60 μl Lipofectamine 2000 (ThermoFisher Scientific, #11668027) diluted in 2 ml Opti-MEM (Gibco cat ...