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Citations for Thermo Fisher :
4101 - 4150 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Plasmid transfection into HEK293T cells was performed using Lipofectamine 2000 (Thermo Fisher Scientific) or polyethylenimine (PEI) ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfection of plasmids and siRNA was performed using Lipofectamine 3000 (Thermo Fisher Scientific) and Lipofectamine RNAiMAX (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cas9 and donor plasmids were co-transfected using Lipofectamine 2000 (Thermo Fisher Scientific) into HEK293E cells which were seeded in a 6-well plate one day before transfection ...
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bioRxiv - Immunology 2020Quote: ... Plasmids encoding heavy and light chains were co-transfected into Expi293F cells (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... along with 3μg pAX2 and 2μg VSVG packaging plasmids using Lipofectamine 3000 (Invitrogen). 48 hours later ...
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bioRxiv - Microbiology 2021Quote: ... PCR products and plasmids were digested with Eco52I and KpnI (Thermo Fisher Scientific), purified using QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: ... After 24 hours cells were transfected with indicated plasmids using Lipofectamine 3000 (Invitrogen) in Opti-MEM™ medium ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids were transiently transfected into CHO cells using ExpiFectamine CHO Transfection Kit (ThermoFisher), and protein was purified one the sixth day post-transfection ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were transiently transfected with EMTB-3XGFP plasmid using the Neon (ThermoFisher Scientific) electroporation system two days before imaging ...
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bioRxiv - Molecular Biology 2022Quote: ... Sense and antisense oligos were annealed and cloned into pENTR/U6 plasmid (Invitrogen). Using LR recombination (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... and plasmids encoding various S genes by using Lipofectamine 3000 (Invitrogen, L3000-015). The supernatants were harvested at 24 ± 2 h post transfection and centrifuged at 1500rpm for 5 min to remove cell debris and then stored at -80°C ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids containing Cas9 and single-guide RNAs were transfected (Lipofectamine 2000; 11668019; Invitrogen) into the LAMP1Δ-RUSH cell line according to manufacturers’ instructions ...
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bioRxiv - Microbiology 2022Quote: ... purified (reference) plasmids were quantified (individually) by fluorometry (Qubit™, Thermo Fisher Scientific), and copy number estimated ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids used for transfection were generated using the Gateway cloning system (Life technologies): transgenes were recombined with the Drosophila Gateway Vector Collection (DGVC ...
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bioRxiv - Biochemistry 2022Quote: ... 50μg of the LentiCRISPRv2 hygro plasmid was digested with BsmBI (ThermoFisher cat # FD0454) and dephosphorylated with FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher cat # EF0654 ...
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bioRxiv - Genomics 2022Quote: ... the plasmid was first digested and dephosphorylated with FastDigest BsmBI and FastAP (Thermofisher) at 37°C for 2 hrs ...
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bioRxiv - Cell Biology 2019Quote: ... The plasmids were introduced into the two cell types using Lipofectamine (Gibco-BRL) according to the manufacturer instruction ...
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bioRxiv - Biophysics 2019Quote: ... were transformed with 100 ng of pET24a His8–MBP plasmid (Thermo Fisher Scientific) encoding the desired HEV constructs and incubated overnight at 37 °C on LB Agar plates containing kanamycin (50 μg/mL) ...
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bioRxiv - Genetics 2020Quote: ... The plasmids were transformed to Stellar™ Competent Cells (Invitrogen, cat. no. 636766) using a standard heat shock protocol (30 min on ice ...
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bioRxiv - Microbiology 2019Quote: ... and transfected with plasmids encoding indicated Gag-YFP derivatives using Lipofectamine 2000 (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The plasmid was introduced into Escherichia coli BL21 Star (DE3) (Life Technologies, USA). The resulting expression strain was pre-grown for 16 hours in liquid culture at 37 °C in LB supplemented with 50 μg ml−1 ampicillin ...
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bioRxiv - Molecular Biology 2019Quote: ... pEGFP-ANK or pEGFP-SET plasmids using Lipofectamine 2000 (11668019, Thermo Fisher Scientific). HDFs were maintained in DMEM supplemented with 10% FBS ...
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bioRxiv - Microbiology 2019Quote: ... 500μL of DMEM containing 4μg plasmid mixed with 10μL Lipofectmine 2000 (Thermo Fisher) was added to each well of the 6-well culture plate when cells grew to 60-70% confluency ...
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bioRxiv - Genomics 2019Quote: ... Ampicillin resistant colonies were collected and extracted for plasmids using Maxiprep kit (Invitrogen). Ten sampling clones from the agar plate were analyzed by PCR and Sanger sequencing to verify successful cassette insertion and barcode diversity (Primer details in Table S1).
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bioRxiv - Bioengineering 2019Quote: ... plasmids were co-transfected into HEK293-T cells using Lipofectamine 2000 (Life Technologies). After 4 h ...
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bioRxiv - Microbiology 2019Quote: ... Constructs were TA-cloned into the gateway entry plasmid pCR8 (Invitrogen; K2500-20). An LR Clonase II reaction (Invitrogen ...
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bioRxiv - Pathology 2020Quote: ... The plasmids were transfected into the HEK-293T cells with Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Plasmids were linearized [75] and transcribed using the MEGAscript T7 Transcription Kit (ThermoFisher) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... or a Flag-control plasmid using Lipofectamine® 2000 Transfection Reagent (ThermoFisher SCIENTIFIC) and cultured for 48h ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were transfected with plasmids using lipofectamine 2000 reagent (Thermo Fisher Scientific #11668019) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... the TRBP ORF was cloned into the pcDNA3.1 and nV5-DEST plasmids (Invitrogen). For the LUMIER-IP and Luciferase assays ...
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bioRxiv - Molecular Biology 2021Quote: ... and 50 ng of mCherry plasmid was then transfected using Lipofectamine 3000 (Invitrogen). For PE3 experiments ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with expression plasmids using lipofectamine transfection reagent (Thermo Fisher Scientific), or infected at an MOI of 1 TCID50/cell ...
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bioRxiv - Microbiology 2020Quote: ... coli mlaF or mlaB strains were transformed with a pBAD-derived plasmid (Invitrogen) encoding WT mlaF or mlaB genes ...
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bioRxiv - Microbiology 2021Quote: HEK293T were transfected with the indicated mammalian expression plasmids using Lipofectamine 2000 (Invitrogen). Twenty-four hours post-transfection ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μg plasmids were mixed with transfection reagents using Lipofectamine 3000 (Life Technologies) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmid DNA was mixed with transfection reagent in Opti-MEM (Thermo Fisher Scientific) and incubated for 30 minutes before addition to cells ...
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bioRxiv - Neuroscience 2019Quote: ... Transfection of the HCN4 overexpression plasmid was done using Lipofectamine 3000 reagent (Invitrogen) and transfection of the Stealth RNAi siRNAs against HCN4 [Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... and transfection of plasmids was performed with Lipofectamine 2000 (Thermo Fisher Scientific, 11668019), both according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 2.5 µg of plasmid DNA were transfected per well using Lipofectamine 3000 (Invitrogen), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... were transfected with plasmid DNA using TurboFect Transfection reagent (ThermoFisher scientific, Courtabeuf, France) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... plasmids were transfected using JetPrime from Polyplus (U2OS) or 293fectin from Gibco (293F) as per manufacturer’s protocols ...
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bioRxiv - Genetics 2020Quote: Reporter plasmids were assembled using the 3-fragment MultiSite Gateway system (ThermoFisher Scientific). Promoters of candidate genes were PCR amplified from N2 genomic DNA ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmids were delivered at equal ratio by lipofection with Lipofectamin Stem (Invitrogen). After G418 selection (100 μg/ml ...
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jouvence, a new human H/ACA snoRNA involves in the control of cell proliferation and differentiationbioRxiv - Genetics 2020Quote: ... the h-jou was cloned in the pcDNA3.1 Zeo + plasmid (ThermoFisher Scientific, USA) between EcoRI sites ...
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bioRxiv - Developmental Biology 2020Quote: The following plasmids were generated by MultiSite Gateway Cloning (Invitrogen, Waltham, US-MA) based on the Tol2kit [Kwan et al. ...
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bioRxiv - Neuroscience 2020Quote: ... 0.7 μg of plasmid was diluted in 100 μL of OptiMEM medium (Gibco). In parallel ...
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bioRxiv - Evolutionary Biology 2021Quote: The backbone plasmid vector pUC19 (Norrander et al. 1983) (ATCC 37254) from ThermoFisher Scientific (SD0061 ...
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bioRxiv - Biochemistry 2021Quote: ... The pET-911 plasmid was transformed into BL21(DE3) codon plus cells (Invitrogen) and plated on LB plus 100 μg Amp ...
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bioRxiv - Immunology 2021Quote: ... and clone 2 hole light chain plasmid) with ExpiFectamine 293 transfection kit (Thermofisher). The expression and antibody purification protocol was similar to recombinant antibody expression described as above ...