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Citations for Thermo Fisher :
4051 - 4100 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... pcDNA3.1-Sox2 plasmids were constructed and transfected using Lipofectamine 3000 (Thermo Fisher Scientific).The expression levels of nestin (forward primer ...
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bioRxiv - Neuroscience 2020Quote: ... and 24h after plasmid transfection using Lipofectamine® LTX and Plus reagent (Invitrogen). siRNA sequences used are ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids were transformed and grown in subcloning efficiency DH5α competent cells (Invitrogen # 18265017) before extraction by Miniprep (QIAGEN #27104 ...
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bioRxiv - Cancer Biology 2021Quote: ... Coli competent cells and amplified using the GeneJET Plasmid Maxiprep Kit (Thermo Scientific). Diagnostic PCR with the MIGR1 primers set ...
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bioRxiv - Cancer Biology 2020Quote: ... Reporter plasmids were transfected at 200ng per 12-well with lipofectamine 3000 (Invitrogen), following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... Resulting plasmids were introduced into E.coli DH5α competent cells (ThermoFisher, Breda, the Netherlands) by heat shock transformation ...
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bioRxiv - Cell Biology 2021Quote: ... and the respective construct plasmid using TurboFect (Thermo Fisher Scientific, Waltham, MA, USA). After 48 hours ...
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bioRxiv - Synthetic Biology 2021Quote: ... Expression constructs were assembled from pENTR plasmids using Gateway cloning (Thermo Fisher Scientific). All plasmids are described in Supplementary Table 3.
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bioRxiv - Genetics 2020Quote: ... LentiCas9-puro-sGArid1a or LentiCas9-Blast plasmid by Lipofectamine 3000 (Life Technologies # L3000015). Virus containing medium was collected at 60h after transfection ...
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bioRxiv - Neuroscience 2020Quote: ... 0.25 μg of plasmid DNA and 0.375 μl Lipofectamine™ 2000 (#743517, Invitrogen) were mixed for one well of a 24-well plate ...
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bioRxiv - Microbiology 2020Quote: STm SL1344-GFP (with pFPV25.1 plasmid) was maintained under Ampicillin selection (11593027, Gibco). STm SL1344 wildtype strain was maintained in the presence of streptomycin (11860038 ...
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bioRxiv - Molecular Biology 2020Quote: ... The plasmid was transfected into the cells using Lipofectamine 3000 (Thermo Fisher Scientific) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: Plasmids were transfected into mammalian cells by using Lipofectamine 3000 (Invitrogen, cat# L3000150). After 24 h transfection ...
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bioRxiv - Synthetic Biology 2021Quote: ... Destination plasmids were grown in One Shot ccdB survival cells (Thermo Fisher Scientific). Transformations were performed in accordance with the manufacturer’s specifications ...
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bioRxiv - Cancer Biology 2019Quote: ... Parental 690.cl2 cells were transfected with that plasmid using Lipofectamine (Life Technologies) following manufacturer’s instructions and GFP positive cells were single sorted into 96 well plates after 24 h ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid DNA and siRNA transfections were performed using lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2021Quote: ... The lentiviral plasmid was transfected along with the ViraPower lentiviral expression system (ThermoFisher), following the recommended protocol from the vendor ...
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bioRxiv - Cell Biology 2021Quote: HeLa or HEK293T cells were transfected with plasmids using Lipofectamine 3000 (ThermoFisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Transfection of plasmids was performed by using lipofectamine 2000™ transfection reagent (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: PX459-sgRNA plasmid (2μg) was transfected using 10 μL of Lipofectamine 2000 (Invitrogen). After 4 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then transfected with the following plasmid concentrations using lipofectamine 2000 (Invitrogen): p1A1-FL (244 ng/well) ...
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bioRxiv - Molecular Biology 2022Quote: ... Transfections of plasmids were performed with Lipofectamine® LTX and Plus Reagent (Invitrogen), siRNAs were transfected using Lipofectamin RNAiMAX ...
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bioRxiv - Cell Biology 2022Quote: The pCMV-Gaussia-Dura Luc and pTK-Red Firefly Luc plasmids from ThermoFisher were used to construct the dual-luciferase reporter plasmid (pGL-RF) ...
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bioRxiv - Biochemistry 2022Quote: ... plasmids were transfected into HEK293 cells in triplicate using Lipofectamine 2000 (Thermofisher Scientific) in the presence of various amounts of DHT ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant human PRKACA cDNA was obtained from Addgene (Plasmid #23495) and cloned into between BamHI and KpnI in pcDNA5 vector (Invitrogen). All final clones were verified using full DNA sequence analysis.
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bioRxiv - Biochemistry 2022Quote: ... Each well was transfected with 360 ng of plasmid using Lipofectamine 3000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... pBv2-U6-6X and pBv1-U6-BB plasmids using Neon Transfection System (Invitrogen) under condition of 1050 V ...
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bioRxiv - Biochemistry 2022Quote: ... and the plasmid was introduced in BL21-star (DE3) cells (Thermo Fisher Scientific). GroEL expression was induced by adding 0.4 mM IPTG to the culture medium when the OD600 reached 0.65 ...
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bioRxiv - Biochemistry 2022Quote: ... DNA plasmids were introduced into cells using Lipofectamine 3000 (Life Technologies, Carlsbad, CA), at a concentration of 1 μg/well (3 μL of L-3000 and 2 μL P3000 ...
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bioRxiv - Cell Biology 2022Quote: ... coli DH5α and extracted by using PureLink™ HiPure Plasmid Miniprep Kit (Invitrogen). Antibodies were expressed using the ExpiCHO™ expression system (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids expressing fusion protein were transiently transfected into Expi293F cells (Thermo Fisher Scientific) using polyethyleneimine (Polysciences) ...
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bioRxiv - Microbiology 2022Quote: ... GFP plasmid is EGFP cloned into pcDNA DEST40 vector (Thermo Fisher Scientific 12274015). RIG-I plasmid DNA was generated by (Dittmann et al. ...
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bioRxiv - Microbiology 2022Quote: ... or N SARS-CoV-2 proteins were cloned into pVAX1 plasmid (Thermo Fisher) as previously described [18] ...
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bioRxiv - Cell Biology 2022Quote: ... MEF cells were transiently transfected with plasmids above using Lipofectamine 3000 (Invitrogen, USA) following the manufacturer’s protocol and grown overnight on a round glass-bottom dish (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... and c-Flag pcDNA3/ERα/ERβ plasmids using Lipofectamine 3000 (Thermo Fisher Scientific) following manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were transfected with 600ng total plasmid DNA using Lipofetamine 3000 reagent (Invitrogen). For experiment shown in Fig 1 ...
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bioRxiv - Bioengineering 2022Quote: ... cells were transfected with plasmid DNA indicated in Results using Lipofectamine 2000 (Invitrogen). On the day of imaging ...
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bioRxiv - Biophysics 2022Quote: ... The linearized plasmid was in vitro transcribed using MEGAscriptTM T7 Transcription Kit (Invitrogen). The CV-B3-RLuc RNA was then electroporated into H1-Hela+CDHR3 cells using the Gene Pulser XcellTM Electroporation System ...
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bioRxiv - Developmental Biology 2022Quote: ... The recombinant plasmids were introduced into HEK293-EBNA cells (Invitrogen, Carlsbad, CA, USA) using FuGENE 6 transfection reagents (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids were transiently transfected using the ExpiFectamine 293 Transfection Kit and protocol (GIBCO).
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bioRxiv - Biochemistry 2022Quote: The resulting pApoA1-ApoM plasmid was transfected into adherent CHO-S cells (InvitroGen) using PEI (Polyethylenimine ...
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bioRxiv - Immunology 2022Quote: The generated plasmids were transfected into FreeStyle 293-F cells (Thermo Fisher Scientific) using the FreeStyle MAX reagent ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and then purified using the Quick Plasmid Purification Kit (Thermo Fisher Scientific, USA). Antisense probes were synthesized by in vitro transcription (MEGAScript Kit ...
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bioRxiv - Cancer Biology 2022Quote: ... The plasmids were transfected using Lipofectamine™ 2000 Transfection Reagent (Thermo Fisher Scientific) according to manufacture’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Transfection of plasmid (10 µg) or 10-20 nM synthetic siRNA (Life Technologies) was performed using Lipofectamine® 2000 transfection reagent (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... the recombinant plasmids were linearized with PacI before transfection with Lipofectamine 2000 (ThermoFisher) into the adenovirus packaging cell lines HEK293 cells ...
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bioRxiv - Microbiology 2020Quote: ... Vero cells were transfected with 750 ng of plasmids using Lipofectamine 2000 (Invitrogen) in 24-well-plates according to the manufacturer’s recommendations and washed 5 h later.
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bioRxiv - Microbiology 2021Quote: ... and subsequently cloned into the pACYC177 plasmid using the Subcloning Service (Invitrogen, Germany). All plasmids were sequenced to check for the presence of the desired point mutations and to ensure there were no unwanted secondary mutations.
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant BACs were purified using PureLink™ HiPure Plasmid Midiprep Kit (ThermoFisher Scientific) and injected into 1-cell stage zebrafish embryos together with I-SceI (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... Next day 1 μl of reporter plasmid was transfected using Lipofectamine2000 (Life Technologies). After 48 h the cells were lysed and luminescence was measured with Dual-Glo Luciferase assay system (Promega ...