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Citations for Thermo Fisher :
4301 - 4350 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... which is based on the pcDNA3.1 plasmid (Thermo Fisher Scientific, Waltham, MA, USA). Clones with wild-type or mutant genomic inserts were selected and verified by sequencing the cloned DNA fragments ...
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bioRxiv - Immunology 2023Quote: ... IgH and IgL plasmids were co-transfected using polyethyleneimine into 293FreeStyle cells (Invitrogen). Supernatants were screened for anti-HLA reactivity using HLA cytometric bead arrays described below ...
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bioRxiv - Neuroscience 2023Quote: Plasmid transfection was performed using the Lipofectamine 3000 reagent (Invitrogen, ref. L3000-008) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were heat transformed into subcloning efficiency chemically competent DH5α cells (Thermofisher, USA) and purified using endotoxin-free maxiprep kits (Thermofisher ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were transfected with 500 ng plasmid DNA using Lipofectamine 2000 (Invitrogen) with a DNA:Lipofectamine ratio of 1:6 according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... DNA from colonies was isolated with the PureLink Quick Plasmid Miniprep Kit (Invitrogen). DNA was sequenced with the M13 Forward primer.
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bioRxiv - Cell Biology 2024Quote: ... 30-100 ng of plasmid DNA was transfected using Lipofectamine 3000 (ThermoFisher Scientific) according to the manufacturer’s instructions for 5-8 hours ...
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bioRxiv - Biophysics 2024Quote: ... MDCK cells were transfected with respective plasmid DNA using Lipofectamine 2000 (Invitrogen, 11668019). Appropriate selection for positives was done by supplementing culture medium with 800 μg ml−1 Geneticin (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... N2a cell transfections involved purified plasmids and lipofectamine 3000 (Invitrogen, Waltham, MA, USA), following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: GAF-DBD (Table 1) was cloned into pET SUMO plasmid (Invitrogen K300-01) using Gibson Assembly (NEB E5520S ...
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bioRxiv - Biophysics 2024Quote: ... samples were transfected with corresponding plasmids using Lipofectamine 3000 (Invitrogen, cat. no. L3000150) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... Transfection of synTF plasmid constructs was perfored using Lipofectamine 3000 (Thermo Fisher Scientific). 500,000 cells were plated in 6 cm culture plates and transfected the following day with the corresponding ng of synTF plus single stranded filler DNA (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Plasmid inserts were excised by restriction digest and recovered with SizeSelect gels (Invitrogen) before being used to template Poly A tail PCRs ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid constructs were diluted in OPTI-MEM medium (Life Technologies, Waltham, MA, US) and transfections were performed using Lipofectamine 3000 Transfection Reagent (Life Technologies ...
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bioRxiv - Genomics 2024Quote: ... We used packaging plasmids (pLP1, pLP2, and pLP/VSV-G; all from Invitrogen) and pLVX-Luc-IRES-ZsGreen1_ Cat ...
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bioRxiv - Microbiology 2024Quote: ... Huh7OK1 cells were transfected with these plasmids using Lipofectamine LTX (Thermo Fisher Scientific). GFP-positive cells were isolated using a FACSAriaTM cell sorter (BD Bioscience ...
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bioRxiv - Molecular Biology 2024Quote: ... both ATG-mutated plasmids were transformed into MAX Efficiency Stbl2 Competent Cells (Invitrogen). Clones were screened by restriction enzyme digestion and verified by Sanger sequencing using F primers in the pKL backbone upstream of RLBP1 and in exons 5 and 7 of RLBP1 ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... PCR products were cloned into the pCR8/GW/TOPO TA plasmid (Thermo Fisher) and then shuttled into a Gateway-converted pCDNA3.1 mammalian expression vector (Invitrogen).
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bioRxiv - Microbiology 2024Quote: ... competent cells and further recovered using GeneJET Plasmid Maxiprep Kit (ThermoFisher Scientific, Canada) as per manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... then the plasmid was digested by BamHI-NotI 10U/μL (Thermo Fisher Scientific) restriction enzymes in Fast Digest Buffer for 1hour at 37°C to purify the transgene of interest ...
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bioRxiv - Biochemistry 2024Quote: ... the successful plasmid DNA was transformed into the INVSc1 yeast (Thermo Fisher Scientific) using S.c ...
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bioRxiv - Cancer Biology 2021Quote: ... platform and 80 samples were analyzed using GPL570 (Affymetrix Human Genome U133 Plus 2.0 Array) platform ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-human CD117 known also as cKIT (12-1178-42, Thermo Fisher Scientific, 1:100).
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bioRxiv - Cell Biology 2020Quote: ... Human iPSC lines were maintained in 5% CO2 environment in Essential 8 Medium (Thermo Fisher). For cardiomyocyte differentiation ...
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bioRxiv - Immunology 2021Quote: The IL-2 ELISA was performed using the IL-2 Human ELISA Kit by ThermoFisher scientific according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... The cells were then stimulated with 20 ng/mL of recombinant human IL-4 (Gibco) and 20 ng/mL of recombinant human IL-13 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... T cells were stimulated for 2 days with anti-human CD3/CD28 magnetic Dynabeads (ThermoFisher) at a bead to cell concentration of 1:1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Primary human and mouse fibroblasts were cultured in Minimum Essential Media (MEM, Thermo Fisher Scientific) supplemented with 15% FBS ...
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bioRxiv - Microbiology 2022Quote: ... Epidermal sheets were cultured separately in RPMI with 10% human AB serum (Thermo Fisher Scientific) and 1% Penicillin/Streptomycin/Amphotericin B solution for 48 h ...
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bioRxiv - Neuroscience 2022Quote: Human α-syn fibrils were labelled with Alexa-488 succinimidyl esther (Thermo Fisher Scientific, A20000) following manufacturer’s instructions and the unbound fluorophore was removed with multiple dialysis steps in sterile PBS ...
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bioRxiv - Neuroscience 2019Quote: ... human iPSC-derived astrocytes were incubated with the calcium indicator Fluo-4 AM (1μM; ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... human fetal lung tissues were dissociated with Dispase (8 U/ml Thermo Fisher Scientific, 17105041) at room temperature (RT ...
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bioRxiv - Immunology 2021Quote: ... Anti-human TIGIT PE Cy 7 (1:50, clone MBSA43, Invitrogen, cat # 25-9500-42), Anti-human CD94 APC (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Secreted IL-1β was measured with the Human IL-1β cytoset ELISA (Invitrogen; Cat.# CHC1213) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Human embryonic kidney (HEK) 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) and supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2019Quote: ... and then stained with Alexa 647-conjugated anti-human IgG (H+L) antibody (Thermo Fisher) for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: The human keratinocytes N/TERT-1 cells (27) were cultured in keratinocyte SFM media (Thermofisher) at 37°C under 5% CO2 atmosphere ...
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bioRxiv - Immunology 2019Quote: ... and human/mouse TGF beta 1 ELISA Ready-SET-Go! Kit (2nd Generation; Affymetrix, eBioscience), respectively ...
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bioRxiv - Cell Biology 2019Quote: ... GSE41258 was generated using the GPL96 platform for transcriptome analysis (Affymetrix Human Genome U133A array), while the other datasets were generated using the GPL570 platform (Affymetrix Human Genome U133Plus 2.0 arrays) ...
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bioRxiv - Cell Biology 2019Quote: The human dynein complex was expressed and purified from insect cells (ExpiSf9 cells; Life Technologies) as previously described with minor modifications72 ...
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bioRxiv - Systems Biology 2019Quote: ... Biotinylated probes were hybridized to GeneChip Human Gene 1.0 ST Arrays (Affymetrix, Santa Clara, CA) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... human foetal lung tissues were dissociated using Dispase (8 U/ml Thermo Fisher Scientific, 17105041) at room temperature (RT ...
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bioRxiv - Cell Biology 2020Quote: ... was obtained from Abcam (Cat.# ab9498) (Cambridge, MA, USA) and anti-human ZO-1 antibody was obtained from Invitrogen / ThermoFisher (Carlsbad ...
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bioRxiv - Developmental Biology 2021Quote: ... Mouse and human sperm were loaded with Cal-520-AM (5 μM, Molecular Probes, USA) in the presence of Pluronic F-127 (0.02% v/v ...
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bioRxiv - Immunology 2021Quote: ... human cGAS was labelled with AlexaFluor-488 (AF488) carboxylic acid (succinimidyl ester) (Thermo Fisher Scientific) according to manufacturer’s manuals using a molar ratio of 1:10 at 4°C for 4 h ...
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bioRxiv - Cancer Biology 2020Quote: Lentiviral particles were produced in human 293T cultured in in Dulbecco’s Modified Eagle’s Medium (ThermoFisher), supplemented with 10% Fetal Bovine Serum (EuroBio ...
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bioRxiv - Bioengineering 2021Quote: ... We then stimulated T cells with Dynabeads™ Human T-Activator CD3/CD28 from ThermoFisher overnight with a ratio of beads to cells as 1:3 ...
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bioRxiv - Bioengineering 2020Quote: ... Human cell lines PC-3 (ATCC® CRL-1435™) were grown in RPMI1640 (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2020Quote: Relative quantitation of secreted Aβ40 was performed with commercial human Aβ40 ELISA kit (Invitrogen, #KHB3481) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... One ng/mL recombinant human interleukin (IL)-6 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), was added to the media of the IL-6 dependent myeloma cell lines INA-6 ...