Labshake search
Citations for Thermo Fisher :
4251 - 4300 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... 250 ng/ml 4′,6-diamidino-2′-phenylindole dihydrochloride (DAPI) or 500 nM SYTOX Orange (Life Technologies, Thermo Fisher Scientific, Schwerte, Germany) were used as nucleic acid stain.
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bioRxiv - Bioengineering 2022Quote: ... Dam serum and placental tissue lysates (both prepared as described above) were assayed for IL-6 concentration using an ELISA per manufacturer instructions (Invitrogen, Waltham, MA). Sample absorbance values were compared to a standard curve to calculate IL-6 concentration.
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bioRxiv - Cell Biology 2022Quote: ... 2’,7’-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated goat anti-mouse and rabbit IgG antibodies were purchased from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Systems Biology 2023Quote: ... Macromolecule extractions performed on this cell line as described typically yielded between 450 - 500 µl of soluble extract at 6 - 8 mg/ml of protein as assessed by Bradford assay (Thermo Fisher, #23200). After target capture ...
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bioRxiv - Microbiology 2022Quote: ... in PBS prior to staining with DAPI (4=,6-diamidino-2-phenylindole) and mounted in Prolong Diamond antifade (Molecular Probes, Life Technologies). Images were captured using a Nikon Eclipse Ti confocal microscope (Nikon Instruments ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative PCR analysis was conducted on an Applied Biosystems QuantStudio™ 6 Flex Real-Time PCR System with Power SYBR Green PCR Master Mix (Applied Biosystems). Expression levels were determined with the delta Ct method and normalized to GAPDH mRNA ...
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bioRxiv - Cancer Biology 2022Quote: ... Culture medium was replaced with lentivirus-containing medium at the ratio of 100 μL virus-containing medium per 25,000 cells seeded in the presence of 6 μg/mL polybrene (Thermo Fisher Scientific #TR1003G), followed by centrifugation at 2,000 rpm in room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... and 60°C for 20 seconds) in a QuantStudio TM 6 Flex Fast Real-Time PCR System (Life Technologies, Grand Island, NY). Q- PCR was conducted in triplicate for each sample.
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bioRxiv - Cell Biology 2023Quote: ... Act5C-GS>Ras attP-GFP-LI-Clone 3 and Act5C-GS>Ras attP-GFP-LB-Clone 6 cultures were grown in the same basal media supplemented with 10 nM of Mifepristone (Thermofisher Cat# H11001). Cultures were allowed to grow in T-25 flasks to become confluent before treatment with trypsin (Gibco Cat# 12604013 ...
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bioRxiv - Cell Biology 2023Quote: ... coated 6-well plates at 5×105 cells/well in RPMI 1640 with B27 and 10% knockout serum replacement (Thermo Fisher Scientific). Cells were expanded by culturing in RPMI 1640 with B27 with 2 μM of CHIR99021 (Stemcell Technologies) ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from 6 pairs of P21 mouse testes (3 pairs of wild type and 3 pairs of Mov10-/-) using TRIzol reagents (Thermo Fisher Scientific). 1 μg of total RNA from each sample was used to generate RNA-seq libraries using TruSeq Stranded mRNA Library Preparation Kit Set A (Cat ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were transfected with 2ug per well (6 well plate) and 1ug per well (12 well plate) of plasmids concentration with the transfection reagent Lipofectamine 3000 (Invitrogen™, L3000001) and the protocol mentioned in the kit was followed ...
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bioRxiv - Biochemistry 2023Quote: ... Crosslinking was reversed for 6 h at 70 °C and samples were treated with 200 μg/ml RNase A (Thermo Scientific, EN0531) and 200 μg/ml proteinaseK (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Fixed cells were stained with 4’,6-diamidino-2-phenylindole (DAPI) for visualization of host-cell nuclei and anti-MOMP antibody conjugated to FITC (Thermo Scientific™) for visualization of Chlamydia ...
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bioRxiv - Biophysics 2022Quote: ... EVs were stained with 5-(and-6)-Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE, hereinafter referred as CFSE) (Thermo Fisher, Catalog No. C1157) and separated as described previously [10] ...
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bioRxiv - Molecular Biology 2023Quote: ... 3D artificial muscles were fixed with buffered 1% paraformaldehyde (PFA) (ThermoScientific, JI9943.K2) overnight at 4°C followed by 6 hours of blocking at 4°C [10% FBS (Gibco, 10270-106), 1% BSA (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... qPCR was performed using the SYBR Green Master Mix (Quanta) and specific gene primers on QuantStudio 6 Flex system (Thermo Fisher Scientific). Relative mRNA levels were determined by normalized to Ribosomal Protein S18 (Rps18 ...
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bioRxiv - Genomics 2023Quote: ... nuclei were counterstained with ProLong Gold anti-fade reagent with 4’,6-diami-dino-2-phenylindole (DAPI) (Life Technologies, catalog no. P36935). Images were viewed on a Zeiss Axioskop 2 using AxioVision Rel ...
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bioRxiv - Cell Biology 2023Quote: ... qPCR was performed using a Quanstudio™ 6 as per the manufacturer’s instructions using TaqMan® gene expression assays (ThermoFisher Scientific, Australia) and normalized to the ribosomal RNA (18s ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 2 micrograms of either CELSR constructs or empty vector DNA in a 6-well plate (Fisher Scientific FB012927) using a 1:3 mixture of LipoD293 (SignaGen Laboratories SL100668) ...
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bioRxiv - Cell Biology 2023Quote: Spheroids in suspension at day 4 were labelled with 4uM EdU and incubated for 6 h using Click-iT EdU Alexa fluor 488 imaging kit (Thermo Fisher Scientific) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... Macromolecule extractions performed on these cell lines as described typically yielded between 450∼500 μl of soluble extract at 6∼8 mg/ml of protein as assessed by Bradford assay (Thermo Fisher, #23200). After target capture ...
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bioRxiv - Microbiology 2023Quote: ... qPCR was performed on a Thermo Fisher Scientific QuantStudio 6 machine using Fast SYBR™ Green Master Mix (Thermo Fisher Scientific 4385618). Expression data is presented after calculating the relative expression compared with the housekeeping gene RPLP0 ...
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bioRxiv - Cancer Biology 2023Quote: ... 6-well plates) siRNA and 10 μL (384-well plates) or 500 μL (6-well plates) Lipofectamine RNAiMAX in OptiMEM (Gibco, 51985-034) were incubated for 20 min at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... Relative enrichment levels were determined by ChIP-qPCR in an optical 384-well plate in the QuantStudio™ 6 Flex Real-Time PCR System (ThermoFisher Scientific), using FastStart Universal SYBR Green Master (Rox ...
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bioRxiv - Cell Biology 2023Quote: ... were treated with 10 µM EdU for 6 hours before dissociation and staining using the Click-It EdU Alexa Fluor 488 Flow Cytometry Assay kit (ThermoFisher, Cat# C10425).
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bioRxiv - Bioengineering 2023Quote: ... The slides were thoroughly rinsed and sealed utilizing a SlowFade Gold antifade reagent with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) and coverslips.44 The stained slides were imaged at 4x magnification with an EVOS fluorescence imaging system.
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bioRxiv - Neuroscience 2023Quote: ... Nuclei was then isolated using a sucrose gradient and ultracentrifugation at 13,000 rcf for 45 mins at 4°C (Sorvall WX-100, F65L-6 x 13.5 rotor, Thermo Fisher, Waltham, MA USA). Isolated nuclei were resuspended in Nuclei PURE Prep kit storage buffer and RNAase inhibitor ...
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bioRxiv - Microbiology 2023Quote: ... Cells were seeded at a density of 106 cells/well or 8×105 cells/well in a 6-well or 96-well tissue culture plate (Nunc; Thermo Fisher Scientific), respectively ...
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bioRxiv - Immunology 2023Quote: ... Quantitative analysis of IL-6 and IFNB expression in THP1 dual reporter cells was performed by StepOne Real-Time PCR Systems (Thermo Fisher Scientific) by using 18S rRNA for normalization ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylation of Htz1(V126C) was carried out using N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)propionamide (HPDP-Biotin) (Thermo Fisher cat. # 21341), which has a pyridyl disulfide moiety ...
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bioRxiv - Molecular Biology 2023Quote: The undifferentiated (Undiff.) and differentiated (Diff.) SH-SY5Y cells seeded in 6 well plates were homogenized in T-PER reagent (ThermoFisher, Cat# 78510), 1% Triton X-100 ...
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bioRxiv - Genetics 2023Quote: Chemical selections were conducted as follows: G418 selection was conducted for 6 days in 150 μg/mL G418/culture medium (#11811023; Thermo Fisher Scientific) 2 days after transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... 6 μl of RNA from each fraction was converted to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher #4368814). The cDNA was diluted 10 times before qPCR analysis ...
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bioRxiv - Biochemistry 2023Quote: ... –6 blotting force and 15 s wait time and flash-frozen into liquid ethane with a Vitrobot Mark IV (Thermo Fisher Scientific). The data was collected on a Titan Krios at the Pacific Northwest Cryo-EM Center (PNCC ...
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bioRxiv - Molecular Biology 2023Quote: ... coated 6-well plates or 18-mm round coverslips in Minimum Essential Media (MEM) with L-glutamine (Thermo Fisher Scientific, cat# 11095080), 0.6% glucose (Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were blocked in 6% BSA and immunolabeled with anti-α3 antibody (mouse Anti-ATP1A3 xVIF 9-G10, MA3-915, Invitrogen, Thermo Fisher scientific) for 2 hours 200 times in PBS1x containing 1%BSA or at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... The coupon was incubated in a 300 nM (100 ng/mL) solution of DAPI (4’,6-diamidino-2-phenylindole, ThermoFisher, Waltham, MA) for 3 minutes as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and cytokine levels in the supernatant/PBS wash quantified using ELISA kits according to manufacturer’s instructions (murine IL-1β: Invitrogen 88-7013-22, murine IL-6: Invitrogen 88-7064-88) and a SpectraMax M2 plate reader ...
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bioRxiv - Physiology 2024Quote: ... sections were incubated for 5 min with 4’,6-diamidino-2-phenylindole, dihydrochloride (DAPI, Dojindo, D523) and then mounted with PermaFluor (Thermo Fisher Scientific). Images were acquired using a BC43 or LSM800 instrument equipped with a Zeiss Axio Observer Z1 and a LSM 800 confocal unit with Airyscan module ...
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bioRxiv - Cancer Biology 2024Quote: Twenty-five thousand MDA-231 or D2A1 cells were seeded in the wells of a 6-well plate and cultured in Phenol free DMEM media (ThermoFisher, catalog# 31053028) supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2024Quote: ... the cell debris was sedimented using ultracentrifugation at 100000xg (SorvallTM WX+, Thermo Scientific with A-27 fixed angle rotor 6 x 50mL). The supernatant was then filtered using 0.2 nm syringe cap filters ...
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bioRxiv - Cell Biology 2024Quote: ... and the same number of cells were re-plated in 6-well plates and selected with G418 (800 µg/ml) (11811031, Thermo Fisher scientific) for 8-14 days ...
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bioRxiv - Bioengineering 2024Quote: ... the supernatant was first processed by centrifugation at 500 and 2000 × g for 5 min each and 10,000 × g for 20 min using a SorvallTM LYNX 6000 centrifuge equipped with a FiberliteTM F12-6 × 500 LEX fixed-angle rotor (both Thermo Fisher Scientific). All centrifugation steps were performed at 4 °C ...
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bioRxiv - Biophysics 2024Quote: ... Neuro2A-Piezo1ko cells were transiently transfected 40-48 hours prior to recording in 6-well plates using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were co-stained with Draq5 (Cell Signal, 4048L) or nuclei stain 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI; Thermo Fisher, D1306). Stained samples were imaged using an Olympus FV3000 Confocal Laser Scanning Microscope.
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bioRxiv - Neuroscience 2024Quote: ... Brain extracts containing 60 ug of protein were loaded and separated on 6-10% denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride (PVDF) membrane (Cat#88518; Thermo Fisher Scientific). The membrane was blocked with 5% milk in Tris-buffered saline with 0.1% Tween (TBST ...
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bioRxiv - Neuroscience 2024Quote: ... iNeurons were resuspended and plated at 600K/well in PLO-lam coated 6-well plates in Neurobasal medium (NBM; Fisher Scientific, 11570556), supplemented with 200 mM GlutaMAX (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... Samples were concentrated onto the trap column at 5 µl/min for 6 min with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap 480™ (Thermo Fisher Scientific) at 300 nl/minute via the analytical column ...
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bioRxiv - Immunology 2024Quote: ... qPCR was performed using a PowerUp SYBR Green Master Mix and standard protocols on an Applied Biosystems QuantStudio 6 Real-Time PCR System (Thermo Fisher Scientific). RPL19 was used to normalize RNA isolated from mouse samples ...