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Citations for Thermo Fisher :
4051 - 4100 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... the aRNA was treated with 6 μL EXO-SAP (ExoSAP-IT™ PCR Product Cleanup Reagent, Thermo Fisher Scientific, Cat. # 78200.200.UL) at 37°C for 15 min followed by fragmentation with 5.5 μL fragmentation buffer (200 mM Tris-acetate (pH 8.1) ...
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bioRxiv - Molecular Biology 2019Quote: ... 3 μl of FuGENE® 6 and 1.3 μg of plasmid were added to 100 μl of OPTI-MEM Medium (Thermo Fisher Scientific). The mixture was incubated at room temperature for 30 minutes prior to the addition to the coverslips.
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected with 4-6 μg linearized targeting vector DNA (ROSA26-SpCas9) using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, USA), selected with 1400-1600 μg/mL G418 (Genaxxon Bioscience ...
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bioRxiv - Biophysics 2019Quote: ... Cells were harvested by centrifugation at 7,800 g for 10 minutes in an F9 6×1000 LEX rotor (Thermo Scientific, Waltham MA), using a Lynx 6000 centrifuge (Thermo Scientific ...
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bioRxiv - Genetics 2020Quote: ... Primer pairs producing a single product band of expected size during PCR amplification were shortlisted for fluorescent dye labelling (forward primers) with one of four fluorescent dyes (6-FAM, VIC, NED, or PET, Invitrogen, South Korea) to perform fragment analysis using Applied Biosystems 3130 Genetic Analyser ...
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bioRxiv - Cell Biology 2019Quote: ... SYBR Green qPCR experiments were performed following the manufacturer’s protocol using a QuantStudio 6 Flex Real-time PCR system (Applied Biosystems, Waltham, MA). cDNA (30 ng ...
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bioRxiv - Developmental Biology 2019Quote: Samples were divided into two groups— peripheral blood- and cord blood-derived cells— such that each set could be separately labeled with TMT 6-plex isobaric mass tagging reagents (Thermo Fisher Scientific) as previously described (41) ...
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bioRxiv - Microbiology 2019Quote: ... medium supplemented with 10 µg/ml gentamicin was added to HIEC-6 cells grown to confluency on 12 mm coverslips (Thermo Fisher Scientific) in 24-well plates (Corning Inc. ...
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bioRxiv - Microbiology 2019Quote: ... isolated for each sediment-depth explored along SSK42/5 and 6 were shotgun sequenced individually on an Ion Proton sequencing platform (Thermo Fisher Scientific) using 200 nucleotide read-chemistry ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Colorimetric protein assays were conducted using commercial human IL-8 ELISA and mouse IL-6 ELISA kits (Invitrogen; 88-8086, 88-7064) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2019Quote: ... the MerMAID1 gene was subcloned with a C-terminal TEV protease restriction site and a 6× His-Tag into the pPiCZ vector (Invitrogen, Carlsbad, CA). Zeocin™-resistant positive clones were selected from electroporation-transformed yeast cells ...
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bioRxiv - Cell Biology 2020Quote: ... The dried peptides were dissolved with 100 mM triethylammonium bicarbonate and then labeled with 6-plex Tandem Mass Tags (TMT) reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... and cells were harvested 6 or 24 hours post-treatment by removal of the media and addition of TRIzol reagent (Life Technologies, UK). RNA was extracted using the RNA Purelink kit (Life Technologies ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from liver tissues of SHR and WKY (4-6 weeks old) using the TRIzol reagent (Invitrogen, Waltham, USA) followed by purification using Nucleospin columns (Machery-Nagel ...
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bioRxiv - Genomics 2019Quote: ... We incubated this mixture at 72 °C for 3 min for denaturing followed by a quick chill on ice and adding the remaining 6 µl reverse transcription mix that contained 2 µl of 5x First Strand Buffer (Thermo Fisher Scientific), 0.1 µl of 100 µM SMARTer II oligo (5’-AAGCAGTGGTATCAACGCAGAGTACATrGrG+G where “+” indicates a locked nucleic acid (LNA ...
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bioRxiv - Biophysics 2019Quote: ... the final dye to protein ratio was typically 5-6 dye molecules per protein molecule as measured with a Nanodrop spectrophotometer (Thermo Fisher Scientific). Cells in suspension culture dishes were labeled with 0.5 nM fluorescent IgE for typically 12-20 hours before imaging.
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bioRxiv - Cell Biology 2019Quote: ... conjugated with GFP at the C-terminus (Par-6-GFP and aPKC-GFP) was inserted into pAc5.1/V5-His B plasmid (Invitrogen, Thermo Fisher Scientific). To construct an expression vector for Par-3 under control of the Gal4-UAS system ...
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bioRxiv - Synthetic Biology 2020Quote: ... cells from 2-D or 3-D cultures were harvested from one well of a 6-well plate and dissociated with Accutase (Thermo Fisher #A1110501), washed three times with 1x PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... which consisted of 2.6 μl NFW, 5 μl TATAA SYBR Green mix (TATAA, Sweden) and 0.4 μl of 10 μM primers (Thermo Fisher Scientific, USA). Cycling protocol consisted of initial enzyme activation at 95°C (t = 3 min) ...
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bioRxiv - Cancer Biology 2021Quote: ... Viral supernatants were derived by transient co-transfection of 293T (6×106 in 100 mm3 petri-plates) cells using lipofectamine 2000 (Thermo Fisher Scientific). A total of 19.5 μg of plasmid in the proportion of 12 μg of lentiviral plasmid carrying human/murine FAP-α cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 × 105 cells were seeded on Type I Collagen-coated 6-well dishes and cultured until day fourteen in DMEM/F12+GlutaMAX medium supplemented with 10% FBS (Life Technologies, #16140071). Vitamin D3 (100 nM ...
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bioRxiv - Cell Biology 2020Quote: ... The mEGFP-tagged Tom20 line was transfected with mCherry-Mito-7 construct (Michael Davidson, addgene #55102) using 6 μl per well of transfection mixture containing 25 μl Opti-MEM (ThermoFisher #31985-070), 1.5 μl GeneJuice (Millipore #70967 ...
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bioRxiv - Immunology 2021Quote: ... 500 ng of RNA was used for the RT-PCR and the qPCR was performed using QuantStudio 6 PCR System (Thermo Fisher Scientific). The primer sequences were as follows ...
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bioRxiv - Cell Biology 2020Quote: S1R was expressed with an N-terminal 6*His-tag fusion in Sf9 cells using Bac-to-Bac baculoviral expression system (Thermo Fisher Scientific) according to manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2020Quote: ... A 1:6 dilution of the PageRuler Plus pre-stained protein ladder (10 µl) was used as a molecular weight standard (ThermoFisher Scientific, Inc.). BSA and human plasma samples were included on each gel for comparison ...
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bioRxiv - Biochemistry 2020Quote: ... 6 to 8-week old male mice (C57BL/6J) were injected via tail vein with 29 G syringe (ThermoFisher 14-841-32) at a dose of 10e11 GC/mouse diluted in saline in a total volume of 100 μl/mouse ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) as counterstaining.
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bioRxiv - Cancer Biology 2021Quote: Cells were grown on 6-well plates until 80-90% confluency and cell surface proteins were biotinylated with EZ-Link NHS-PEG4-Biotin (ThermoFisher Scientific, #21330) at 0.5 mg/ml in HBSS (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR was performed using QuantStudio™ 6 Flex real-time PCR system with power SYBR® green PCR master mix (Applied Biosystems). All gene expression data were normalized by GAPDH using the comparative CT method (2-ΔΔCt) ...
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bioRxiv - Biochemistry 2021Quote: ... The mixture was left to equilibrate for 5 min at RT before 6 μL aliquots were placed in crystal-clear vials (Fisher Scientific UK) and snap-frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... one well of a 6 well plate was transfected with 2 μg of plasmid DNA with Lipofectamine® 2000 (Life Technologies™) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR experiment was performed on the QuantStudio 6 Flex Real-Time PCR Systems using the following Taqman probes: actin (Applied Biosystems, 4331182), GAPDH (Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were seeded and transfected with the sgMCL1-expressing plasmid in a 6 well plate using Lipofectamine LTX transfection reagent (Cat# 15338030, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The electrophoresis of amplified products was performed on 6% acrylamide gels at 160 Volts for 30 minutes (Thermo Electron Electrophoresis EC250-90, Thermo Fisher Scientific). The results were visualized under UV light using UVITEC Imaging Systems (Cleaver Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed three times with PBS and mounted on the slide with ProLong Diamond antifade with 4′,6-diamidino-2-phenylindole (DAPI; Life Technologies, #P36966). Confocal images were acquired on a Nikon Ti-Eclipse A1M microscope fitted with a 60× oil immersion objective using 488 nm ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were washed again in PBS for 3×15 min and mounted in DAPI (4-,6-diamidino-2-phenylindole; Life Technologies, D1306) for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... was introduced into 5 × 106 C6/36 mosquito cells per well of a 6-well plate via Lipofectamine 2000 reagent (Thermo Fisher Scientific), following the product’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were subsequently plated on islands of glial cells at a density of 1.25 × 104 cells/cm2 in 6-well plates in Neurobasal-A supplemented with 2% B27 and 0.2% penicillin/streptomycin (Invitrogen/ Thermo Fisher, Waltham, MA). Lentiviral particles encoding synaptoPAC were added 1–4 days after plating ...
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bioRxiv - Synthetic Biology 2021Quote: HEK293T cells cultured in 6-well plates were washed with PBS and harvested by adding 600 μl TRIzol (Life Technologies cat#15596026) directly to the cells ...
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bioRxiv - Physiology 2021Quote: ... U2OS cells were transfected in 6-well dishes with 2ug of pSpCas9(BB)-2A-GFP plasmid containing the respective sgRNA using Lipofectamine 2000 (Life Technologies, 11668027). After 24h incubation ...
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bioRxiv - Physiology 2021Quote: ... The cells were seeded onto one well of a Synthemax-treated 6-well plate and in StemFlex media containing 1X RevitaCell (ThermoFisher, Cat # A2644501), 30 mM HDR Enhancer (IDT) ...
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bioRxiv - Physiology 2021Quote: ... qPCR was performed in triplicate for each sample on the QuantStudio 6 Pro Real-Time PCR System (Applied Biosystems, Waltham, MA, USA) using TaqMan Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... ATPase activity was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside–purine nucleoside phosphorylase reaction to detect released inorganic phosphate (EnzChek kit; Thermo Fisher Scientific) (62) ...
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bioRxiv - Plant Biology 2021Quote: Initial validation of the flg22 treatment was assessed with real-time (RT) quantitative polymerase chain reactions (qPCR) on a QuantStudio 6 instrument (Thermo Fisher Scientific), using the total RNA extracted for the CAGE library preparations and the ΔΔCt method ...
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bioRxiv - Microbiology 2020Quote: ... sections of liver biopsies were performed as described previously (6) using a pan-genotype probeset (genotype A-D, VF6-20095, Affymetrix, CA. USA). Sections were stained with NBT (Nitroblue tetrazolium ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR conditions were applied according to the manufacturer’s instructions using QuantStudio 6 Flex Real-Time PCR System (ThermoFisher Scientific, Waltham, Massachusetts, MA). HPRT or Glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were renatured by dialyzing out urea in a step wise manner from 6 M in steps of two at room temperature using Slide–A-Lyzer MINI Dialysis units (Thermo Scientific, USA) with a 10,000 Dalton MWCO ...
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bioRxiv - Cancer Biology 2021Quote: ... 6 × 105 of isolated MLEC were cultured in a 4-well chamber slide (Nunc Lab-Tek Chamber Slide System, Thermo Fisher Scientific) precoated with Extracellular Matrix Attachment Solution provided in the kit ...
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bioRxiv - Cancer Biology 2021Quote: ... Approximately 1×106 of A375 and A375 IV cells were electroporated with 6 μg of plasmid DNA utilizing Neon Transfection System (Thermo Fisher Scientific) 1200V ...
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bioRxiv - Cancer Biology 2022Quote: ... 5x105 cells of the primary hepatocyte were seeded in a 6-well plate (matrigel coated) with 2mL Advanced DMEM/F-12 (Thermo Fisher, 12634010) supplemented with 10% fetal calf serum ...