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Citations for Thermo Fisher :
4451 - 4500 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... A total volume of 10µl per reaction was run on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems Thermo Fisher Scientific, 4485691) for 45 cycles (2 minutes at 50 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were blocked in 6% BSA and immunolabeled with anti-α3 antibody (mouse Anti-ATP1A3 xVIF 9-G10, MA3-915, Invitrogen, Thermo Fisher scientific) for 2 hours 200 times in PBS1x containing 1%BSA or at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... The concentrations of cdM2-1 and RNA were determined by absorbance at 280 and 260 nm using extinction coefficients of 5,960 M−1·cm−1 (Expasy-ProtParam) (14) and 8,050 M−1·cm−1·base−1 (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2019Quote: ... ZO-1 (1:100, ThermoFisher), vimentin (1:100 ...
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bioRxiv - Bioengineering 2020Quote: ... 1:1,(GIBCO, Life Technologies) medium supplemented with 10% fetal bovine serum(GIBCO) ...
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bioRxiv - Bioengineering 2020Quote: ... 1:1,(GIBCO, Life Technologies) medium supplemented with 10% fetal bovine serum(GIBCO) ...
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bioRxiv - Neuroscience 2019Quote: ... 1:1 Neurobasal (ThermoFisher; 21103049) with 1× Glutamax ...
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bioRxiv - Cell Biology 2023Quote: ... 1% HEPES (1 M, Gibco), 1% L-glutamine (200 mM ...
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bioRxiv - Cell Biology 2022Quote: ... 1% HEPES 1 M (Gibco), 1% Sodium Pyruvate 100 mM (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... ZO-1 (1:100, ThermoFisher), E-Cadherin (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... and 1:1 3-methyl-1-butanol (Thermo Fisher Scientific) in mineral oil were used as cues ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1% antibiotics in DMEM/F12 (1:1) media (Gibco).
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bioRxiv - Immunology 2022Quote: ... Zonula occludens-1 (ZO-1, 1:250 Invitrogen 33-9100), and Cav-1 (1:500 Invitrogen PA5-17447) ...
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bioRxiv - Neuroscience 2024Quote: ... cells were switched to neural induction media (DMEM/F12 and Neurobasal medium at 1:1 ratio, 1% B27, 0.5% N2, 1% PenStrep, 1% Glutamax; ThermoFisher Scientific) supplemented with ascorbic acid (0.1 mM ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR was performed with 2x PowerUP™ SYBR™Green Master Mix in the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems). GAPDH (AT1G13440 ...
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bioRxiv - Microbiology 2020Quote: Cells in 6- and 12-well format were washed with PBS and treated with 10 mM TrypLE™ Express Enzyme (Gibco; Cat. No. 12605028). Cells were then collected with PBS and added to 10% PFA to a final concentration of 4% ...
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bioRxiv - Cell Biology 2020Quote: ... Harvested equine explants were allowed to recover in 6-well plates for 3-7 days in the following control medium: DMEM/F12 medium (11330, Thermo Fisher Scientific, Waltham, MA), 10% fetal bovine serum (Seradigm 1500-500 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) reactions were carried out using the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, Life Technologies) with the SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... 105 rabbit Sf1 epithelial cells were plated into each well of a 6-well plate and incubated at 37°C overnight in a 5% CO2 atmosphere within a HeraCell 150 incubator (Thermo Fisher Scientific, Waltham, MA). At the same time ...
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bioRxiv - Immunology 2021Quote: Human T cells were differentiated for 6–7 days in a humidified 5% CO2 incubator at 37°C with IMDM (Thermo Fisher, Waltham, MA, USA) supplemented with 10% fetal bovine serum ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... The cells are then transferred with warm hNSC medium to a 6-well plate coated with Geltrex (Gibco, A1413202, incubate for 1hr at 37C). The day after transfection the medium was refreshed and 1µg/ml puromycin (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... ∼25000 astrocytes were plated per 30 mm glass coverslip in 6-well plates and allowed to form micro-dot islands in DMEM (Thermo Fisher Scientific, Cat #11965092) supplemented with 10% (vol/vol ...
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bioRxiv - Cancer Biology 2020Quote: ... MiRNAs or vectors were transfected in the amount of 2 μg for 6-well plate into indicated cells by Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, Waltham, USA) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: ... Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis of interleukin (IL)-6 was performed using TaqMan Gene Expression Assays (Rn01410330_m1; Applied Biosystems, Foster City, CA, USA) and 7900HT Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Biophysics 2019Quote: ... Bacteria were harvested by centrifugation for 10 minutes at 4800 rpm (Thermo Lynx 4000 F12-6 rotor, Thermo Fisher Scientific, Waltham, MA, United States). The obtained pellets were stored at −80 °C until needed ...
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bioRxiv - Biochemistry 2020Quote: ... Insoluble fraction was removed by high speed centrifugation at 4°C (25658 g for 30 minutes, Thermo Fisher SCIENTIFIC #A23-6 x 100 rotor). The supernatant was filtrated with 0.22 μm filter (Filtropur BT50 0.2 ...
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bioRxiv - Bioengineering 2020Quote: ... the coverslips were placed upside down on a glass slide with slow fade mounting medium containing 4’,6-diamidino-2- phenylindole (DAPI, Life Technologies, Carlsbad, CA, USA) and kept at 4 °C until observation ...
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bioRxiv - Cancer Biology 2021Quote: ... the 5’ end of the forward primer sequences for RheBAT26 and RheD18S58 MSI markers was labeled with a 6-FAM fluorescent dye (Thermo Fisher Scientific, Waltham, MA). A multiplex PCR was designed to amplify RheBAT26 and RheD18S58 MSI markers with labeled primer sequences ...
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bioRxiv - Plant Biology 2020Quote: ... 20 ng of reverse transcribed RNA was used as template in the real-time PCR using Applied Biosystems(tm) QuantStudio(tm) 6 Flex (Fisher Scientific GmbH, Schwerte, Germany). Specific primers binding the 3’-UTR of the NOS terminator were used for the detection of the transgene transcripts ...
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bioRxiv - Microbiology 2020Quote: ... were acid digested on a hot plate using 6 mL Primar Plus grade HNO3 (68%) and 2 mL H2O2 (Thermo Fisher Scientific, Loughborough, UK). Samples were diluted with Milli-Q water (18.2 MΩ cm ...
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bioRxiv - Molecular Biology 2020Quote: ... Real-time qPCR reactions were carried on using power up SYBR green master mix on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, Life Technologies Inc.). Ct (cycle threshold ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated at 37°C in normoxia (21% O2) for Balb/c MSC or hypoxia (5% O2) for C57BL/6 MSC in culture medium consisting of MEM-α (ThermoFisher Scientific, Dublin, Ireland) supplemented with 10% heat-inactivated foetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... wound lysate cell suspensions were sorted from Tie-2Cre/CXCL12loxP/loxP and CXCL12loxP/loxP transgenic mice as single progenitor cells into each well of a 96-well plate using a Becton Dickinson FACSAria flow cytometer (Franklin Lakes, NJ) into 6 μl of lysis buffer and SUPERase-In RNAse inhibitor (Applied Biosystems, Foster City, CA) (N=5 mice) ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative real-time PCR was performed in triplicates using the QuantStudio 6 Flex Real-Time PCR System and Taqman Universal PCR Master Mix (Applied Biosystems, Foster City, CA). Probe sets for Il6 (Rn01410330_m1) ...
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FGF10 triggers de novo alveologenesis in a BPD model: impact on the resident mesenchymal niche cellsbioRxiv - Cell Biology 2022Quote: ... at RT and washed three times with TBST before being mounted with Prolong Diamond Anti-fade Mountant with DAPI (Invitrogen 4’,6-diamidino2-phenylindole). Photomicrographs of immunofluorescence staining were taken using a Leica DMRA fluorescence microscope with a Leica DFC360 FX camera (Leica ...
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bioRxiv - Cancer Biology 2022Quote: ... 200’000 cells of selected monocolonies of either cell line were seeded in 6-well plates and transfected on next day with CRISPR plasmids using Lipofectamine 2000 (Invitrogen by Life Technologies #11668019) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was blotted for 25 s with a force of 6 and then flash-frozen in liquid ethane and stored in liquid nitrogen using a Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, USA) with the environmental chamber set at 100% humidity ...
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bioRxiv - Biophysics 2022Quote: Human adipose-derived stem cells (hASCs, passage p<=6) were obtained from the Radboud Biobank and cultured in Minimum Essential Medium Eagle (α-MEM) (Invitrogen, Thermo Fisher, USA). All media were supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... 1.6 μL of the RNA extracts were used for RNA measurement with the use of the NanoDrop™ 2000 Spectrophotometer (Thermo Fisher Scientific, UK). The RNA samples were then stored at -80 °C.
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bioRxiv - Microbiology 2020Quote: ... Then RNA was reverse transcribed into cDNA and the transcriptional levels of different genes were determined by qRT-PCR using SybrGreen Premix Low rox (MDbio, China) and the QuantStudio™ 6 Flex (Thermo Fisher Scientific, America). 16S rRNA was used as an internal reference and the merF gene expression was calculated using the 2−ΔΔCt method ...
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bioRxiv - Microbiology 2021Quote: After the skin flap surgery and 15 min prior to the tail-vein injection of GFP-expressing bacteria (107 CFU in 100 μl 1x PBS) and PE-conjugated anti-human CD62E (6 μg, Thermofisher Scientific, #12-0627-42), 100 μg Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293T cells were seeded into 6 well plates and transfected with 1ug plasmid mixture of pX459-gRNA1 and pX459-gRNA2 using Lipofectamine 2000 (Thermo Fisher Scientific, Cat. 11668019). On the next day ...
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bioRxiv - Cell Biology 2022Quote: ... Library quantification was performed using the Collibri™ Library Quantification Kit (Thermo Fischer Scientific, A38524500) in a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific).Both batches were sequenced in a NovaSeq6000 sequencer (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... Gene expression was measured using real time qPCR with PowerUp SYBR Green Master mix on a QuantStudio 6 instrument (Thermo Fisher Scientific, Waltham, MA). Results were normalized to RPS9 (ribosomal protein S9 ...
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bioRxiv - Microbiology 2019Quote: ... A total of 6 μg of total RNA was reverse-transcribed with random primers using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA). qRT-PCR was conducted using the Applied Biosystems One-Step Real-time PCR protocol using SYBR Green fluorescence (Applied Biosystems ...
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bioRxiv - Bioengineering 2020Quote: A single colony of the desired strain was inoculated in 25 mL pH 6 buffered Glycerol-complex (BMGY) medium (Pichia Expression Kit, Life Technologies, Carlsbad, CA, USA) and grown at 28 °C with 250 RPM shaking until saturation ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Pathology 2021Quote: ... the cells also were washed with PBS and transferred to either aIL-6 antibody (10 ug/ml) or IgG (10 ug/ml) in serum-free DMEM (Thermo Fisher Scientific, Gibco #11966025) supplemented with 4.5 g/L D-glucose ...
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bioRxiv - Bioengineering 2020Quote: ... of the paraffin-embedded spinal cord tissue blocks were cut at a thickness of 6-7 μm using semi-automatic motorized microtome (HM 355, Thermo Fisher Scientific, Kalamazoo, MI). The sections were stained Cresyl Violet (Nissl stain ...